NSD3促进组蛋白H3K36双甲基化抑制巨噬细胞中TNF-α的产生
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摘要
目的主要研究巨噬细胞中核受体结合SET结构域蛋白3(NSD3)对脂多糖(LPS)触发的肿瘤坏死因子α(TNF-α)的调控作用,并探讨其调控机制。方法以小鼠腹腔巨噬细胞和RAW264. 7细胞作为细胞模型。100 ng/m L LPS刺激小鼠腹腔巨噬细胞,采用实时定量PCR检测NSD3 mRNA水平,Western blot法检测NSD3蛋白水平;在RAW264. 7细胞中过表达NSD3或采用RNA干扰技术敲低腹腔巨噬细胞中NSD3的表达,ELISA检测NSD3对LPS触发的TNF-α分泌的影响; Western blot法检测敲低NSD3对LPS触发的核因子κB p65(NF-κBp65)信号通路的影响;荧光素酶法检测NSD3对NF-κBp65介导的TNF-α基因转录活性的影响;染色质免疫沉淀实验检测TNF-α基因启动子区组蛋白H3的36位赖氨酸(H3K36)甲基化的募集。结果 LPS抑制巨噬细胞中NSD3的表达;过表达NSD3抑制LPS触发的TNF-α的产生,敲低NSD3则促进LPS触发的TNF-α的产生,但对NF-κB活化无影响; NSD3抑制NF-κBp65介导的TNF-α基因的转录活化,促进TNF-α基因启动子区的H3K36二甲基化。结论 NSD3促进TNF-α基因启动子区H3K36的双甲基化,抑制TNF-α的表达。
Objective To investigate the role of nuclear receptor-binding SET domain protein 3( NSD3) in lipopolysaccharide( LPS)-triggered tumor necrosis factor α( TNF-α) production in macrophages and the underlying epigenetic mechanism. Methods The experiment used murine peritoneal macrophages and RAW264. 7 cells as cell models. The mRNA and protein level of NSD3 were detected by real-time quantitative PCR and Western blot analysis in mouse peritoneal macrophages stimulated with LPS( 100 ng/m L). ELISA was used to detect the production of TNF-α in NSD3-overexpressing RAW264. 7 cells or NSD3-silencing peritoneal macrophages. Western blot analysis was performed to test the activation of LPS-triggered NF-κBp65 in NSD3-silencing macrophages. Luciferase assay was used to assess NF-κBp65-mediated transcriptional activation of TNF-α gene. Ch IP assay was used to detect the recruitment of H3 K36 methylation to TNF-α gene promoter. Results LPS inhibited the expression of NSD3 in the macrophages. Over-expression of NSD3 suppressed LPS-triggered TNF-α production,and silencing NSD3 promoted LPS-triggered TNF-α production. However,NSD3 had no effect on the activation of LPS-triggered NF-κBp65. NSD3 enhanced NF-κBp65-mediated transcriptional activation of TNF-αgene. NSD3 enhanced the dimethylation of H3 K36 of TNF-α gene promoter. Conclusion NSD3 can promote the dimethylation of H3 K36 of TNF-α gene promoter and suppress TNF-α transcription and production.
Objective To investigate the role of nuclear receptor-binding SET domain protein 3( NSD3) in lipopolysaccharide( LPS)-triggered tumor necrosis factor α( TNF-α) production in macrophages and the underlying epigenetic mechanism. Methods The experiment used murine peritoneal macrophages and RAW264. 7 cells as cell models. The mRNA and protein level of NSD3 were detected by real-time quantitative PCR and Western blot analysis in mouse peritoneal macrophages stimulated with LPS( 100 ng/m L). ELISA was used to detect the production of TNF-α in NSD3-overexpressing RAW264. 7 cells or NSD3-silencing peritoneal macrophages. Western blot analysis was performed to test the activation of LPS-triggered NF-κBp65 in NSD3-silencing macrophages. Luciferase assay was used to assess NF-κBp65-mediated transcriptional activation of TNF-α gene. Ch IP assay was used to detect the recruitment of H3 K36 methylation to TNF-α gene promoter. Results LPS inhibited the expression of NSD3 in the macrophages. Over-expression of NSD3 suppressed LPS-triggered TNF-α production,and silencing NSD3 promoted LPS-triggered TNF-α production. However,NSD3 had no effect on the activation of LPS-triggered NF-κBp65. NSD3 enhanced NF-κBp65-mediated transcriptional activation of TNF-αgene. NSD3 enhanced the dimethylation of H3 K36 of TNF-α gene promoter. Conclusion NSD3 can promote the dimethylation of H3 K36 of TNF-α gene promoter and suppress TNF-α transcription and production.
引文
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[2]Higgs M R,Sato K,Reynolds J J,et al.Histone methylation by SETD1A protects nascent DNA through the nucleosome chaperone activity of FANCD2[J/OL].Mol Cell,2018,71(1):25-41.e6.DOI:10.1016/j.molcel.2018.05.018.Epub 2018 Jun 21.
[3]Yu R,Wang X,Moazed D.Epigenetic inheritance mediated by coupling of RNAi and histone H3K9 methylation[J].Nature,2018,558(7711):615-619.
[4]Ryan J,Pilkington L,Neuhaus K,et al.Investigating the epigenetic profile of the inflammatory gene IL-6 in late-life depression[J/OL].BMC Psychiatry,2017,17(1):354.DOI:10.1186/s12888-017-1515-8.
[5]Hu Y,Suliman B A.Roles of HDACs in the responses of innate immune cells and as targets in inflammatory diseases[J].Adv Exp Med Biol,2017,1024:91-110.
[6]Shen Q,Zhang Q,Shi Y,et al.Tet2 promotes pathogen infectioninduced myelopoiesis through mRNA oxidation[J].Nature,2018,554(7690):123-127.
[7]Zhang Q,Zhao K,Shen Q,et al.Tet2 is required to resolve inflammation by recruiting HDAC2 to specifically repress IL-6[J].Nature,2015,525(7569):389-393.
[8]Li X,Zhang Q,Ding Y,et al.Methyltransferase Dnmt3a upregulates HDAC9 to deacetylate the kinase TBK1 for activation of antiviral innate immunity[J].Nat Immunol,2016,17(7):806-815.
[9]Vougiouklakis T,Hamamoto R,Nakamura Y,et al.The NSD family of protein methyltransferases in human cancer[J].Epigenomics,2015,7(5):863-874.
[10]Hudlebusch H R,Santoni-Rugiu E,Simon R,et al.The histone methyltransferase and putative oncoprotein MMSET is overexpressed in a large variety of human tumors[J].Clin Cancer Res,2011,17(9):2919-2933.
[11]Kuo A J,Cheung P,Chen K,et al.NSD2 links dimethylation of histone H3 at lysine 36 to oncogenic programming[J].Mol Cell,2011,44(4):609-620.
[12]Yang P,Guo L,Duan Z J,et al.Histone methyltransferase NSD2/MMSET mediates constitutive NF-kappaB signaling for cancer cell proliferation,survival,and tumor growth via a feed-forward loop[J].Mol Cell Biol,2012,32(15):3121-3131.
[13]Wang G G,Cai L,Pasillas M P,et al.NUP98-NSD1 links H3K36methylation to Hox-A gene activation and leukaemogenesis[J].Nat Cell Biol,2007,9(7):804-812.
[14]Papillon-Cavanagh S,Lu C,Gayden T,et al.Impaired H3K36methylation defines a subset of head and neck squamous cell carcinomas[J].Nat Genet,2017,49(2):180-185.
[15]Lu T,Jackson M W,Wang B,et al.Regulation of NF-kappaB by NSD1/FBXL11-dependent reversible lysine methylation of p65[J].Proc Natl Acad Sci U S A,2010,107(1):46-51.
[16]Wang C,Wang Q,Xu X,et al.The methyltransferase NSD3promotes antiviral innate immunity via direct lysine methylation of IRF3[J].J Exp Med,2017,214(12):3597-3610.
[17]Zhang Q,Wang C,Liu Z,et al.Notch signal suppresses Toll-like receptor-triggered inflammatory responses in macrophages by inhibiting extracellular signal-regulated kinase 1/2-mediated nuclear factorκB activation[J].J Biol Chem,2012,287(9):6208-6217.
[18]Sun D,Wen Q,Wang C.KAT6B promotes LPS-triggered IL-6 production via enhancing recruitment of H3K23 acetylation to IL-6 promoter region[J].Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi,2017,33(11):1441-1447.
[19]Ren W,Sun D,Wang C,et al.Brd3 promotes IL-6 production via enhancing acetylase CBP recruitment and histone 3 acetylation within IL6 promoter[J].Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi,2016,32(10):1301-1305.
[20]Song M,Fang F,Dai X,et al.MKL1 is an epigenetic mediator of TNF-α-induced proinflammatory transcription in macrophages by interacting with ASH2[J].FEBS Lett,2017,591(6):934-945.
[21]Zhao S,Zhong Y,Fu X,et al.H3K4 methylation regulates LPS-induced proinflammatory cytokine expression and release in macrophages[J/OL].Shock,2018 Mar 21.DOI:10.1097/SHK.0000000000001141.[Epub ahead of print].
[22]Liu Y,Zhang Q,Ding Y,et al.Histone lysine methyltransferase Ezh1 promotes TLR-triggered inflammatory cytokine production by suppressing Tollip[J].J Immunol,2015,194(6):2838-2846.
[23]Stender J D,Pascual G,Liu W,et al.Control of proinflammatory gene programs by regulated trimethylation and demethylation of histone H4K20[J].Mol Cell,2012,48(1):28-38.
[24]Xu G,Liu G,Xiong S,et al.The histone methyltransferase Smyd2is a negative regulator of macrophage activation by suppressing interleukin 6(IL-6)and tumor necrosis factorα(TNF-α)production[J].J Biol Chem,2015,290(9):5414-5423.
[25]Biggar K K,Li S S.Non-histone protein methylation as a regulator of cellular signalling and function[J].Nat Rev Mol Cell Biol,2015,16(1):5-17.
[26]Biggar K K,Wang Z,Li S S.Lysine methylation beyond histones[J].Mol Cell,2017,68(5):1016-1016.
[27]Park I Y,Powell R T,Tripathi,D N,et al.Dual chromatin and cytoskeletal remodeling by SETD2[J].Cell,2016,166(4):950-962.
[28]Dasgupta M,Dermawan J K,Willard B,et al.STAT3-driven transcription depends upon the dimethylation of K49 by EZH2[J].Proc Natl Acad Sci U S A,2015,112(13):3985-3990.