不同发育时期小鼠心肌MyoG基因的表达分析
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摘要
肌细胞生成素MyoG基因一直是肌肉研究中的一个热点基因,其在肌肉中的表达水平对该肌肉的生长发育至关重要。试验通过HE染色、免疫组织化学和q PCR技术研究了不同发育阶段小鼠心肌中MyoG的表达差异。结果表明,MyoG在幼年、性成熟、体成熟和老年4个时期的小鼠心肌胞质中均有表达,且其随着年龄增长逐渐下降,尤其在老年鼠中表达显著降低。结果提示,MyoG基因在心脏中的表达与心肌生长发育密切相关。
MyoG has always been a hot gene in muscle research, and its expression level in muscle is crucial for the growth and development of muscle. In this study, the expression of MyoG in cardiac muscle of different developmental stages was studied by HE staining, immunohistochemistry and qPCR. The results showed that MyoG was expressed in the myocardial cytoplasm of mice in the four stages of juvenile, sexual maturity, body maturation and old age, and its expression decreased gradually with age, especially in the aged rats. The results suggest that the expression of MyoG gene in the heart is closely related to myocardial growth and development.
MyoG has always been a hot gene in muscle research, and its expression level in muscle is crucial for the growth and development of muscle. In this study, the expression of MyoG in cardiac muscle of different developmental stages was studied by HE staining, immunohistochemistry and qPCR. The results showed that MyoG was expressed in the myocardial cytoplasm of mice in the four stages of juvenile, sexual maturity, body maturation and old age, and its expression decreased gradually with age, especially in the aged rats. The results suggest that the expression of MyoG gene in the heart is closely related to myocardial growth and development.
引文
[1]张涛,张跟喜,张向前,等.京海黄鸡肌细胞生成素基因(MyoG)组织表达谱及表达规律[J].农业生物技术学报,2015,23(5):617-622.
[2] NABESHIMA Y,HANAOKA K,HAYASAKA M,et al. Myogenin gene disruption results in perinatal lethality because of severe muscle defect[J]. Nature(London),1993,364:532-535.
[3]柴志欣,信金伟,王会,等. MyoG基因多态性与牦牛生长性能的关联分析[J].华北农学报,2018,33(6):108-115.
[4] FUJISAWASEHARA A. Development and regeneration of skeletal muscle[J]. Tanpakushitsu Kakusan Koso,2000,45(S1):2228-2234.
[5]白俊艳,杨又兵,王玉琴,等.绵羊MyoG基因外显子1的多态性及其与肉质性状的关联分析[J].中国畜牧兽医,2017,44(6):1778-1783.
[6] WEINTRAUB H,DAVIS R,TAPSCOTT S,et al. The myoD gene family:nodal point during specification of the muscle cell lineage[J].Science,1991,251:761-766.
[7] YABLONKA-REUVENI Z,SEGER R,RIVERA A J. Fibroblast growth factor promotes recruitment of skeletal muscle satellite cells in young and old rats[J]. Journal of Histochemistry&Cytochemistry,1999,47(1):23-42.
[8]赵青,钟土木,徐宁迎.金华猪MyoG基因多态性与部分生长性能的关系[J].华南农业大学学报,2010,31(2):121-124.
[9]贾径,刘贺贺,司建民,等.鸭MyoG基因编码区的克隆及其在鸭心肌组织发育中的表达研究[J].中国畜牧杂志,2010,46(21):1-4.
[10]刘丑生,赵兴波,李宁,等.动物肌肉生长发育调控的功能基因研究进展[J].中国畜牧杂志,2003,39(5):48-49.
[11]栾兆进,贺建宁,程明,等.绵羊肌肉组织MyoG和PID1基因的表达及其与肌内脂肪含量的关系[J].畜牧兽医学报,2016,47(10):1986-1994.
[12]赵青.金华猪生长激素(pGH)与肌细胞生成素基因(MyoG)多态性及其对生长性状影响的研究[D].杭州:浙江大学,2009.
[13] TEPAS M F W,SOUMILLION A,RETTENBERGER G. Characterization of the porcine myogenin gene locus and association between polymorphism and growth traits[J]. Animal Genetics,1996,27(S2):117.
[14]李景芬,刘娣,于浩. 7个猪种MyoD基因家族中3个基因外显子的SNPs检测分析[J].中国畜枚杂志,2005,41(5):21-24.
[15] ERNST C W,MENDEZ E A,ROBIC A,et al. Rapid communication:myogenin(MyoG)physically maps to porcine chromosome9q2.1-q2.6.[J]. Journal of Animal Science,1998,76(1):328.
[16]栾兆进,曲绪仙,贺建宁,等.家畜MyoG基因结构和功能研究进展[J].家畜生态学报,2015,36(7):1-4.
[17]李硕.山羊、牛Myo G启动子序列克隆及其活性分析[D].呼和浩特:内蒙古大学,2013.
[18]刘铮铸,巩元芳,张文香,等.山羊MyoG基因启动子区序列分析与结构预测[J].畜牧兽医学报,2012,43(1):29-36.
[19]王颖薇,秦子夕,武征.心肌补片的制备与结构功能特点[J].中国组织工程研究,2015,19(38):6211-6216.
[2] NABESHIMA Y,HANAOKA K,HAYASAKA M,et al. Myogenin gene disruption results in perinatal lethality because of severe muscle defect[J]. Nature(London),1993,364:532-535.
[3]柴志欣,信金伟,王会,等. MyoG基因多态性与牦牛生长性能的关联分析[J].华北农学报,2018,33(6):108-115.
[4] FUJISAWASEHARA A. Development and regeneration of skeletal muscle[J]. Tanpakushitsu Kakusan Koso,2000,45(S1):2228-2234.
[5]白俊艳,杨又兵,王玉琴,等.绵羊MyoG基因外显子1的多态性及其与肉质性状的关联分析[J].中国畜牧兽医,2017,44(6):1778-1783.
[6] WEINTRAUB H,DAVIS R,TAPSCOTT S,et al. The myoD gene family:nodal point during specification of the muscle cell lineage[J].Science,1991,251:761-766.
[7] YABLONKA-REUVENI Z,SEGER R,RIVERA A J. Fibroblast growth factor promotes recruitment of skeletal muscle satellite cells in young and old rats[J]. Journal of Histochemistry&Cytochemistry,1999,47(1):23-42.
[8]赵青,钟土木,徐宁迎.金华猪MyoG基因多态性与部分生长性能的关系[J].华南农业大学学报,2010,31(2):121-124.
[9]贾径,刘贺贺,司建民,等.鸭MyoG基因编码区的克隆及其在鸭心肌组织发育中的表达研究[J].中国畜牧杂志,2010,46(21):1-4.
[10]刘丑生,赵兴波,李宁,等.动物肌肉生长发育调控的功能基因研究进展[J].中国畜牧杂志,2003,39(5):48-49.
[11]栾兆进,贺建宁,程明,等.绵羊肌肉组织MyoG和PID1基因的表达及其与肌内脂肪含量的关系[J].畜牧兽医学报,2016,47(10):1986-1994.
[12]赵青.金华猪生长激素(pGH)与肌细胞生成素基因(MyoG)多态性及其对生长性状影响的研究[D].杭州:浙江大学,2009.
[13] TEPAS M F W,SOUMILLION A,RETTENBERGER G. Characterization of the porcine myogenin gene locus and association between polymorphism and growth traits[J]. Animal Genetics,1996,27(S2):117.
[14]李景芬,刘娣,于浩. 7个猪种MyoD基因家族中3个基因外显子的SNPs检测分析[J].中国畜枚杂志,2005,41(5):21-24.
[15] ERNST C W,MENDEZ E A,ROBIC A,et al. Rapid communication:myogenin(MyoG)physically maps to porcine chromosome9q2.1-q2.6.[J]. Journal of Animal Science,1998,76(1):328.
[16]栾兆进,曲绪仙,贺建宁,等.家畜MyoG基因结构和功能研究进展[J].家畜生态学报,2015,36(7):1-4.
[17]李硕.山羊、牛Myo G启动子序列克隆及其活性分析[D].呼和浩特:内蒙古大学,2013.
[18]刘铮铸,巩元芳,张文香,等.山羊MyoG基因启动子区序列分析与结构预测[J].畜牧兽医学报,2012,43(1):29-36.
[19]王颖薇,秦子夕,武征.心肌补片的制备与结构功能特点[J].中国组织工程研究,2015,19(38):6211-6216.