水稻镉安全材料的镉吸收动力学特征
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摘要
采用水培试验,以前期筛选出的水稻Cd安全材料D62B为试验材料,普通材料泸恢17为对照材料,在不同Cd浓度和时间处理下,研究水稻Cd安全材料的Cd吸收动力学特性.结果表明:在不同Cd处理时间下,D62B根系对Cd的吸收总量均低于泸恢17,且随吸收时间的延长,差异逐渐增大.当吸收时间达到72 h,泸恢17体内积累的Cd总量为D62B的1.3倍.两种水稻材料Cd吸收动力学符合米氏方程,米氏方程常数(K_m)值差异不大,但泸恢17的最大吸收速率(V_(max))是D62B的2倍.当Cd处理时间大于48 h,D62B根系对Cd的转移系数低于泸恢17,且其根部Cd分配比例明显大于泸恢17,即D62B根系对Cd的固持能力大于泸恢17.D62B的Cd吸收能力较弱,且其向地上部转移Cd的能力显著低于泸恢17.
The characteristics of cadmium(Cd) uptake kinetics in a pollution-safe rice variety of D62B were studied in a hydroponic experiment under different Cd levels and stress time,with a common variety Luhui17 as the control. The results showed that Cd uptake in root of D62B was s ignificantly lower than that of Luhui17 under different stress times. The differences in Cd uptake of the two rice varieties increased with the extension of absorption time. Total Cd amount of Luhui17 was 1.3 times as much as that of D62B when the absorption time was 72 h. Meanwhile,the Cd uptake kinetic of the two varieties accorded to Michaelis-Menten equation,and little difference in Michaelis constants(Km) was observed in the two varieties. However,the maximum uptake rate(Vmax) of Luhui17 was 2 times as much as that of D62B. Once the stress time was more than 48 h,the transfer coefficients of D62B was lower than that of Luhui17,and the Cd distribution ratio in root of D62B was much higher,indicating that D62B had greater accumulation ability in root compared with Luhui17. In conclusion,the Cd uptake and transfer ability of D62B were lower than those of Luhui17.
The characteristics of cadmium(Cd) uptake kinetics in a pollution-safe rice variety of D62B were studied in a hydroponic experiment under different Cd levels and stress time,with a common variety Luhui17 as the control. The results showed that Cd uptake in root of D62B was s ignificantly lower than that of Luhui17 under different stress times. The differences in Cd uptake of the two rice varieties increased with the extension of absorption time. Total Cd amount of Luhui17 was 1.3 times as much as that of D62B when the absorption time was 72 h. Meanwhile,the Cd uptake kinetic of the two varieties accorded to Michaelis-Menten equation,and little difference in Michaelis constants(Km) was observed in the two varieties. However,the maximum uptake rate(Vmax) of Luhui17 was 2 times as much as that of D62B. Once the stress time was more than 48 h,the transfer coefficients of D62B was lower than that of Luhui17,and the Cd distribution ratio in root of D62B was much higher,indicating that D62B had greater accumulation ability in root compared with Luhui17. In conclusion,the Cd uptake and transfer ability of D62B were lower than those of Luhui17.
引文
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[2]Ying R-R(应蓉蓉),Du S-J(杜锁军),Hu P-J(胡鹏杰),et al.Kinetic characteristics of Zn uptake by Potentilla griffithii Hook.f.var.velutina Card.Chinese Journal of Applied Ecology(应用生态学报),2008,19(6):1349-1354(in Chinese)
[3]Epstein E,Hagen CE.A kinetic study of the absorption of alkali cations by barley roots.Plant Physiology,1952,27:457-474
[4]Sterckeman T,Redjala T,Morel JL.Influence of exposure solution composition and of plant cadmium content on root cadmium short-term uptake.Environmental and Experimental Botany,2011,74:131-139
[5]Liu S-Y(刘双营),Li Y-E(李彦娥),Zhao X-L(赵秀兰).Kinetic characteristics of cadmium uptake by different tobacco cultivars.Chinese Agricultural Science Bulletin(中国农学通报),2010,26(5):257-261(in Chinese)
[6]Wang L(王龙),Gao Z-P(高子平),Li W-H(李文华),et al.Genetic diversity of cadmium uptake kinetics in rice seedlings.Plant Physiology Journal(植物生理学报),2016,52(1):125-133(in Chinese)
[7]Li H,Luo N,Zhang LJ,et al.Do arbuscular mycorrhizal fungi affect cadmium uptake kinetics,subcellular distribution and chemical forms in rice?Science of the Total Environment,2016,571:1183-1190
[8]He JY,Zhu C,Ren YF,et al.Root morphology and cadmium uptake kinetics of the cadmium-sensitive rice mutant.Biologia Plantarum,2007,51:791-794
[9]Yoshibumi H,Hiroshi H.Kinetics of cadmium and zinc absorption by rice seedling roots.Soil Science and Plant Nutrition,1984,30:527-532
[10]Zhan J(詹杰),Wei S-H(魏树和),Niu R-C(牛荣成).Advances of cadmium contaminated paddy soil research and new measure of its safe production in China:A review.Journal of Agro-Environment Science(农业环境科学学报),2012,31(7):1257-1263(in Chinese)
[11]Xie LH,Tang SQ,Wei XJ,et al.The cadmium and lead content of the grain produced by leading Chinese rice cultivars.Food Chemistry,2017,217:217-224
[12]Zhang L(张路),Zhang X-Z(张锡洲),Li T-X(李廷轩),et al.Cd uptake and distribution characteristics of Cd pollution-safe rice materials.Scientia Agricultura Sinica(中国农业科学),2015,48(1):174-184(in Chinese)
[13]Zhang L(张路),Zhang X-Z(张锡洲),Li T-X(李廷轩),et al.Effects of cadmium stress on uptake and distribution of cadmium in different rice varieties.Journal of Agro-Environment Science(农业环境科学学报),2014,33(12):2288-2295(in Chinese)
[14]Zhang HJ,Zhang XZ,Li TX,et al.Variation of cadmium uptake,translocation among rice lines and detecting for potential cadmium-safe cultivars.Environmental Earth Sciences,2014,71:277-286
[15]Yu H(于辉),Yang Z-Y(杨中艺),Yang Z-J(杨知建),et al.Chemical forms and subcellular and molecular distribution of Cd in two Cd-accumulation rice genotypes.Chinese Journal of Applied Ecology(应用生态学报),2008,19(10):2221-2226(in Chinese)
[16]Liu H(刘辉),Zhang J(张静),Du Y-X(杜彦修),et al.Responses of rice genotypes with different silicon uptake efficiency to different silicon supply.Chinese Journal of Applied Ecology(应用生态学报),2009,20(2):320-324(in Chinese)
[17]Liu Y(刘媛),Ma W-C(马文超),Zhang W(张雯),et al.,Effect of cadmium stress on root vigor and accumulation of elements Ca,Mg,Mn,Zn,Fe in Salix variegate.Chinese Journal of Applied Ecology(应用生态学报),2016,27(4):1109-1115(in Chinese)
[18]Zhao FJ,Zhao FJ,Jiang RF,et al.Cadmium uptake,translocation and tolerance in the hyperaccumulator Arabidopsis halleri.New Phytologist,2006,172:646-654
[19]Grant CA,Clarke JM,Duguid S,et al.Selection and breeding of plant cultivars to minimize cadmium accumulation.Science of the Total Environment,2008,390:301-310
[20]Li P(李鹏),Ge Y(葛滢),Wu L-H(吴龙华),et al.Uptake and translocation of cadmium and its physiological effects in two rice cultivars differed in grain cadmium concentration.Chinese Journal of Rice Science(中国水稻科学),2011,25(3):291-296(in Chinese)
[21]Liu Z.Research advance on the mechanism of cadmium transport in rice.Meteorological and Environmental Research,2014,5:48-52
[22]Redjala T,Zelko I,Sterckeman T,et al.Relationship between root structure and root cadmium uptake in maize.Environmental and Experimental Botany,2011,71:241-248
[23]Qiu Q,Wang Y,Yang Z,et al.Effects of phosphorus supplied in soil on subcellular distribution and chemical forms of cadmium in two Chinese flowering cabbage(Brassica parachinensis L.)cultivars differing in cadmium accumulation.Food and Chemical Toxicology,2011,49:2260-2267
[24]Wang X,Liu Y,Zeng G,et al.Subcellular distribution and chemical forms of cadmium in Bechmeria nivea(L.)Gaud.Environmental and Experimental Botany,2008,62:389-395
[25]Wu Z,Zhao X,Sun X,et al.Xylem transport and gene expression play decisive roles in cadmium accumulation in shoots of two oilseed rape cultivars(Brassica napus).Chemosphere,2015,119:1217-1223
[26]Luo J-W(罗洁文),Huang M-Y(黄玫英),Yin D-Y(殷丹阳),et al.Uptake kinetic characteristics and subcellular distribution of Pb2+and Cd2+in Neyraudia reynaudiana.Journal of Agro-Environment Science(农业环境科学学报),2016,35(8):1451-1457(in Chinese)
[27]Lu L-L(卢玲丽).Cadmium Uptake and Translocation in the Hyperaccumulator Sedum alfredii H.Ph D Thesis.Hangzhou:Zhejiang University,2009(in Chinese)
[28]Zhao FJ,Hamon RE,Lombi E,et al.Characteristics of cadmium uptake in two contrasting ecotypes of the hyperaccumulator Thlaspi caerulescens.Journal of Experimental Botany,2002,53:535-543
[29]Yamaguchi N,Mori S,Baba K,et al.Cadmium distribution in the root tissues of solanaceous plants with contrasting root-to-shoot Cd translocation efficiencies.Environmental and Experimental Botany,2011,71:198-206
[30]Mori S,Uraguchi S,Ishikawa S,et al.Xylem loading process is a critical factor in determining Cd accumulation in the shoots of Solanum melongena and Solanum torvum.Environmental and Experimental Botany,2009,67:127-132
[31]Lux A,Martinka M,Vaculík M,et al.Root responses to cadmium in the rhizosphere:A review.Journal of Experimental Botany,2011,62:21-37
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