羊全脂乳粉对小鼠肠道菌群的影响
|
摘要
中医书籍中有多处羊乳有益肠道健康的记载,这可能与羊乳能够影响肠道菌群有关。为研究羊全脂乳粉对小鼠肠道菌群的影响,本研究收集饲喂羊全脂乳粉小鼠的盲肠内容物,采用CTAB法提取肠道菌群总DNA,经PCR扩增16s rDNA V4区后,用Illumina高通量测序技术测定小鼠肠道中菌群的分布和丰度状况,并重点分析小鼠肠道中乳杆菌属、双歧杆菌属、拟杆菌属、梭菌属的分布,并结合小鼠血液生化指标分析来研究羊全脂乳粉对小鼠肠道菌群和生理生化指标的影响。
According to the record of Traditional Chinese Medicine,goat milk was beneficial to the health which may be related to its effect on the intestinal microbiota.The influence of goat milk powder on intestinal microbiota in mice was studied by CTAB extracting DNA,PCR amplification of 16s rDNA V4 region,then Illumina high-throughput sequencing in this investigation.The distribution of Bacteroides,Clostridium,Lactobacillus,Bifidobacterium was the focus.The blood physiological and biochemical parameters were also been analyzed to obtain the influence of goat milk powder.
According to the record of Traditional Chinese Medicine,goat milk was beneficial to the health which may be related to its effect on the intestinal microbiota.The influence of goat milk powder on intestinal microbiota in mice was studied by CTAB extracting DNA,PCR amplification of 16s rDNA V4 region,then Illumina high-throughput sequencing in this investigation.The distribution of Bacteroides,Clostridium,Lactobacillus,Bifidobacterium was the focus.The blood physiological and biochemical parameters were also been analyzed to obtain the influence of goat milk powder.
引文
[1]MARTINEZ-FEREZ A,RUDLOFF S,GUADIX A,et al.Goats’milk as a natural source of lactosederived oligosaccharides:Isolation by membrane technology[J].International Dairy Journal,2006,16(2):173-181.
[2]孙常文,周清涛,王超,等.低聚半乳糖概述及其功能效果原理分析[J].食品安全导刊,2015,10(4):57-58.
[3]周达,范建高.肠道菌群-SCFAs在代谢性疾病中的作用研究[J].胃肠病学和肝病学杂志,2016,25(3):330-332.
[4]徐万里,陆高,梁世杰,等.短链脂肪酸介导的菌群-宿主互动与肠易激综合征的研究进展[J].世界华人消化杂志,2015,23(36):5815-5822.
[5]WHISNER C M,MARTIN B R,SCHOTERMAN MH C,et al.Galacto-oligosaccharides increase calcium absorption and gut bifidobacteria in young girls:a double-blind cross-over trial[J].British Journal of Nutrition,2013,110(7):1292-1303.
[6]WATSON D,O′CONNELL MOTHERWAY M,SCHOTERMAN M H C,et al.Selective carbohydrate utilization by lactobacilli and bifidobacteria[J].Journal of Applied Microbiology,2013,114(4):1132-1146.
[7]谢婷,夏金荣,高峻,等.低聚半乳糖对应激大鼠肠黏膜屏障功能的影响[J].第二军医大学学报,2014,35(1):55-60.
[8]DROR T,DICKSTEIN Y,DUBOURG G,et al Microbiota manipulation for weight change[J].Microbial Pathogenesis,2017,106:146-161.
[9]CHEN F,WEN Q,JIANG J,et al.Could the gut microbiota reconcile the oral bioavailability conundrum of traditional herbs[J].Journal of Ethnopharmacology,2016,179:253-264.
[10]TSUKUMO D M,CARVALHO B M,CARVALHOFILHO M A,et al.Translational research into gut microbiota:new horizons on obesity treatment:up dated 2014[J].Archives of Endocrinology and Metabolism,2015,59(2):154-160.
[11]YAMAMOTO H,KAIDA S,YAMAGUCHI T,et al.Potential mechanisms mediating improved glycemic control after bariatric/metabolic surgery[J].Surgery Today,2016,46(3):268-274.
[12]WIRTH R,B魷DI N,MAR魷TI G,et al.Regionally distinct alterations in the composition of the gut microbiota in rats with streptozotocin-induced diabetes[J].PloS One,2014,9(12):e110440.
[13]ZHANG Z,LIU Z.Paneth cells:the hub for sensing and regulating intestinal flora[J].Science China Life Sciences,2016,59(5):463-467.
[14]OWCZAREK D,RODACKI T,DOMAGA覵A-RO-DACKA R,et al.Diet and nutritional factors in inflammatory bowel diseases[J].World Journal of Gastroenterology,2016,22(3):895.
[15]吴海,周赛男,郭纯,等.一种基于PCR分析多样性的小鼠肠道微生物宏基因组提取方法[J].中国微生态学杂志,2012,24(7):648-651.
[16]李丽婷,许文涛,郭星,等.提取鼠肠道内微生物基因组DNA的方法研究[J].中国粮油学报,2010,25(1):122-127.
[17]YANG Y,YANG S C,ZHAO J,et al.Microbial diversity in Paris polyphylla var.yunnanensis rhizomes of varying ages[J].Genetics and Molecular Research,2015,14(4):17612-17621.
[18]LI L T,YAN B L,LI S H,et al.A comparison of bacterial community structure in seawater pond with shrimp,crab,and shellfish cultures and in non-cultured pond in Ganyu,Eastern China[J].Annals of Microbiology,2016,66(1):317-328.
[19]BELOBRAJDIC D P,MCINTOSH G H,OWENS JA.A high-whey-protein diet reduces body weight gain and alters insulin sensitivity relative to red meat in wistar rats[J].The Journal of Nutrition,2004,134(6):1454-1458.
[20]ZEMEL M B.Role of calcium and dairy products in energy partitioning and weight management[J].The American Journal of Clinical Nutrition,2004,79(5):907S-912S.
[21]秦立强,王培玉,李伟,等.牛奶及其制品与2型糖尿病关系的研究进展[J].卫生研究,2009,4(38):499-501.
[22]张奇男.喝牛羊奶不会增加胆固醇[J].当代畜禽养殖业,1994,(5):30.
[23]ALFREZ M J,BARRIONUEVO M,L魷PEZALIAGA I,et al.Digestive utilization of goat and cow milk fat in malabsorption syndrome[J].Journal of Dairy Research,2001,68(3):451-461.
[24]LPEZ-ALIAGA I,ALF魪REZ M J M,NESTARESM T,et al.Goat milk feeding causes an increase in biliary secretion of cholesterol and a decrease in plasma cholesterol levels in rats[J].Journal of Dairy Science,2005,88(3):1024-1030.
[25]DAZ-CASTRO J,ALF魪REZ M J M,L魷PEZ-ALIAGA I,et al.Bile composition,plasma lipids and oxidative hepatic damage induced by calcium supplementation;effects of goat or cow milk consumption[J].Journal of Dairy Research,2013,80(2):246-254.
[2]孙常文,周清涛,王超,等.低聚半乳糖概述及其功能效果原理分析[J].食品安全导刊,2015,10(4):57-58.
[3]周达,范建高.肠道菌群-SCFAs在代谢性疾病中的作用研究[J].胃肠病学和肝病学杂志,2016,25(3):330-332.
[4]徐万里,陆高,梁世杰,等.短链脂肪酸介导的菌群-宿主互动与肠易激综合征的研究进展[J].世界华人消化杂志,2015,23(36):5815-5822.
[5]WHISNER C M,MARTIN B R,SCHOTERMAN MH C,et al.Galacto-oligosaccharides increase calcium absorption and gut bifidobacteria in young girls:a double-blind cross-over trial[J].British Journal of Nutrition,2013,110(7):1292-1303.
[6]WATSON D,O′CONNELL MOTHERWAY M,SCHOTERMAN M H C,et al.Selective carbohydrate utilization by lactobacilli and bifidobacteria[J].Journal of Applied Microbiology,2013,114(4):1132-1146.
[7]谢婷,夏金荣,高峻,等.低聚半乳糖对应激大鼠肠黏膜屏障功能的影响[J].第二军医大学学报,2014,35(1):55-60.
[8]DROR T,DICKSTEIN Y,DUBOURG G,et al Microbiota manipulation for weight change[J].Microbial Pathogenesis,2017,106:146-161.
[9]CHEN F,WEN Q,JIANG J,et al.Could the gut microbiota reconcile the oral bioavailability conundrum of traditional herbs[J].Journal of Ethnopharmacology,2016,179:253-264.
[10]TSUKUMO D M,CARVALHO B M,CARVALHOFILHO M A,et al.Translational research into gut microbiota:new horizons on obesity treatment:up dated 2014[J].Archives of Endocrinology and Metabolism,2015,59(2):154-160.
[11]YAMAMOTO H,KAIDA S,YAMAGUCHI T,et al.Potential mechanisms mediating improved glycemic control after bariatric/metabolic surgery[J].Surgery Today,2016,46(3):268-274.
[12]WIRTH R,B魷DI N,MAR魷TI G,et al.Regionally distinct alterations in the composition of the gut microbiota in rats with streptozotocin-induced diabetes[J].PloS One,2014,9(12):e110440.
[13]ZHANG Z,LIU Z.Paneth cells:the hub for sensing and regulating intestinal flora[J].Science China Life Sciences,2016,59(5):463-467.
[14]OWCZAREK D,RODACKI T,DOMAGA覵A-RO-DACKA R,et al.Diet and nutritional factors in inflammatory bowel diseases[J].World Journal of Gastroenterology,2016,22(3):895.
[15]吴海,周赛男,郭纯,等.一种基于PCR分析多样性的小鼠肠道微生物宏基因组提取方法[J].中国微生态学杂志,2012,24(7):648-651.
[16]李丽婷,许文涛,郭星,等.提取鼠肠道内微生物基因组DNA的方法研究[J].中国粮油学报,2010,25(1):122-127.
[17]YANG Y,YANG S C,ZHAO J,et al.Microbial diversity in Paris polyphylla var.yunnanensis rhizomes of varying ages[J].Genetics and Molecular Research,2015,14(4):17612-17621.
[18]LI L T,YAN B L,LI S H,et al.A comparison of bacterial community structure in seawater pond with shrimp,crab,and shellfish cultures and in non-cultured pond in Ganyu,Eastern China[J].Annals of Microbiology,2016,66(1):317-328.
[19]BELOBRAJDIC D P,MCINTOSH G H,OWENS JA.A high-whey-protein diet reduces body weight gain and alters insulin sensitivity relative to red meat in wistar rats[J].The Journal of Nutrition,2004,134(6):1454-1458.
[20]ZEMEL M B.Role of calcium and dairy products in energy partitioning and weight management[J].The American Journal of Clinical Nutrition,2004,79(5):907S-912S.
[21]秦立强,王培玉,李伟,等.牛奶及其制品与2型糖尿病关系的研究进展[J].卫生研究,2009,4(38):499-501.
[22]张奇男.喝牛羊奶不会增加胆固醇[J].当代畜禽养殖业,1994,(5):30.
[23]ALFREZ M J,BARRIONUEVO M,L魷PEZALIAGA I,et al.Digestive utilization of goat and cow milk fat in malabsorption syndrome[J].Journal of Dairy Research,2001,68(3):451-461.
[24]LPEZ-ALIAGA I,ALF魪REZ M J M,NESTARESM T,et al.Goat milk feeding causes an increase in biliary secretion of cholesterol and a decrease in plasma cholesterol levels in rats[J].Journal of Dairy Science,2005,88(3):1024-1030.
[25]DAZ-CASTRO J,ALF魪REZ M J M,L魷PEZ-ALIAGA I,et al.Bile composition,plasma lipids and oxidative hepatic damage induced by calcium supplementation;effects of goat or cow milk consumption[J].Journal of Dairy Research,2013,80(2):246-254.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |