HPLC法同时测定涤痰丸中7种成分
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摘要
目的建立HPLC法同时测定涤痰丸(牵牛子、大黄、黄芩)中没食子酸、儿茶素、咖啡酸、大黄酸、大黄素、阿魏酸、黄芩苷的含有量。方法该药物甲醇提取液的分析采用Agilent ZORBAX SB-C_(18)色谱柱(250 mm×4. 6 mm,5μm);流动相甲醇-0. 1%磷酸,梯度洗脱;体积流量1. 0 mL/min;柱温30℃;检测波长254、278、320 nm。结果 7种成分在各自范围内线性关系良好(r> 0. 999 5),平均加样回收率98. 74%～100. 95%,RSD0. 86%～1. 84%。结论该方法简便准确,重复性好,可用于涤痰丸的质量控制。
AIM To establish an HPLC method for the simultaneous content determination of gallic acid,catechin,caffeic acid,rhein,emodin,ferulic acid and baicalin in Ditan Pills( Pharbitidis Semen,Rhei Radix et Rhizoma,Scutellariae Radix). METHODS The analysis of methanol extract of this drug was performed on a 30 ℃thermostatic Agilent ZORBAX SB-C_(18)column( 250 mm × 4. 6 mm,5 μm),with the mobile phase comprising of methanol-0. 1% phosphoric acid flowing at 1. 0 mL/min in a gradient elution manner, and the detection wavelengths were set at 245,320,278 nm. RESULTS Seven constituents showed good linear relationships within their own ranges( r > 0. 999 5),whose average recoveries were 98. 74%-100. 95% with the RSDs of0. 86%-1. 84%. CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Ditan Pills.
AIM To establish an HPLC method for the simultaneous content determination of gallic acid,catechin,caffeic acid,rhein,emodin,ferulic acid and baicalin in Ditan Pills( Pharbitidis Semen,Rhei Radix et Rhizoma,Scutellariae Radix). METHODS The analysis of methanol extract of this drug was performed on a 30 ℃thermostatic Agilent ZORBAX SB-C_(18)column( 250 mm × 4. 6 mm,5 μm),with the mobile phase comprising of methanol-0. 1% phosphoric acid flowing at 1. 0 mL/min in a gradient elution manner, and the detection wavelengths were set at 245,320,278 nm. RESULTS Seven constituents showed good linear relationships within their own ranges( r > 0. 999 5),whose average recoveries were 98. 74%-100. 95% with the RSDs of0. 86%-1. 84%. CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Ditan Pills.
引文
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[11]罗文华,田莹莹,李新辉,等. HPLC法同时测定健儿消食口服液中毛蕊异黄酮苷、橙皮苷和黄芩苷的含量[J].药物分析杂志,2015,35(11):1919-1923.
[12]赵亮,王新霞,吕磊,等. HPLC法测定清肝散结颗粒中黄芩苷、汉黄芩苷、野黄芩苷、黄芩素及汉黄芩素[J].中成药,2014,36(2):313-318.
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[2]程玉英.中西医结合治疗冠心病稳定性心绞痛临床观察[J].山西职工医学院学报,2012,22(3):49-51.
[3]梁淋渊,甘麦邻,罗燕.大黄化学成分与药理活性研究进展[J].中兽医医药杂志,2017,36(1):80-83.
[4]傅兴圣,陈菲,刘训红,等.大黄化学成分与药理作用研究新进展[J].中国新药杂志,2011,20(16):1534-1538,1568.
[5]吉晓丽.黄芩的化学成分与药理作用研究进展[J].中医临床研究,2017,9(9):128-129.
[6]郑勇凤,王佳婧,傅超美,等.黄芩的化学成分与药理作用研究进展[J].中成药,2016,38(1):141-147.
[7]张晶.牵牛子药理、毒副作用及临床应用研究探讨[J].中外医疗,2009,28(12):176.
[8]华之卉,刘栋,李明春. HPLC法同时测定麻杏抗感颗粒中5种成分[J].中成药,2017,39(12):2525-2528.
[9]颜永刚,尹立敏,王红艳,等. HPLC法同时测定大黄叶中9种成分[J].中草药,2016,47(13):2360-2364.
[10]许乾丽,茅向军,宋晓宁,等. HPLC法同时测定六味安消胶囊中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的含量[J].药物分析杂志, 2010, 30(10):1841-1844.
[11]罗文华,田莹莹,李新辉,等. HPLC法同时测定健儿消食口服液中毛蕊异黄酮苷、橙皮苷和黄芩苷的含量[J].药物分析杂志,2015,35(11):1919-1923.
[12]赵亮,王新霞,吕磊,等. HPLC法测定清肝散结颗粒中黄芩苷、汉黄芩苷、野黄芩苷、黄芩素及汉黄芩素[J].中成药,2014,36(2):313-318.
[13]侯学智,张振秋,尤春雪,等. HPLC测定黄芩中6个成分的含量[J].中国现代应用药学, 2012,29(11):1010-1014.