基于荧光纳米颗粒免疫层析技术的毒死蜱快筛试纸的研制
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摘要
目的研制快速检测中药材中毒死蜱的快筛试纸。方法采用免疫层析技术,以CdTe@SiO2荧光纳米颗粒为标记物制备毒死蜱单抗-荧光纳米颗粒偶联物,以竞争抑制模式制备毒死蜱荧光免疫层析试纸条。结果该试纸条对中药中毒死蜱的检出限为25 ng·g~(-1)。从开始制样到检测出结果仅需15～20 min。结论该法操作简便,具有较高的灵敏度和特异性,可广泛应用于中药材中毒死蜱残留的现场筛查和检测。
OBJECTIVE To make chlorpyrifos test strip for on-site rapid screening and identification of chlorpyrifos in traditional Chinese medicine. METHODS CdTe@SiO2 fluorescent nanoparticle conjugated covalently with monoclonal antibody against chlorpyrifos was employed in the development of lateral flow strip assay by using the immunofluorescence technique. RESULTS This rapid test strip had a detection limit as low as 0.25 ng·kg~(-1). It was easy of use and only takes15-20 min to finish the test. CONCLUSION It is very simple in operation, high in sensitivity and specificity, and can be widely used in Chinese medicine screening and detection of chlorpyrifos residue.
OBJECTIVE To make chlorpyrifos test strip for on-site rapid screening and identification of chlorpyrifos in traditional Chinese medicine. METHODS CdTe@SiO2 fluorescent nanoparticle conjugated covalently with monoclonal antibody against chlorpyrifos was employed in the development of lateral flow strip assay by using the immunofluorescence technique. RESULTS This rapid test strip had a detection limit as low as 0.25 ng·kg~(-1). It was easy of use and only takes15-20 min to finish the test. CONCLUSION It is very simple in operation, high in sensitivity and specificity, and can be widely used in Chinese medicine screening and detection of chlorpyrifos residue.
引文
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[3]XIA G F.Apolication of QuEchERS in pesticide residue analysis of traditional Chinese medicine[D].Guangzhou:South China Agricultural University,2016.
[4]HU L,LI Y H,ZHOU M H.Determination of organophosphorus pesticide residue in health food with plant or fungus as materials by GC[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(4):563-566.
[5]何晖,王炳志,毕思远,等.一种快速筛查志贺氏菌的荧光纳米免疫层析方法的建立[J].食品安全导刊,2016(9):115-119.
[6]YU J,SU J,ZHANG J,et al.CdTe/CdS quantum dot-labeled fluorescent immunochromatography test strips for rapid detection of Escherichia coli O157:H7[J].RSC Advances,2017,7(29):17819-17823.
[7]XIANG H,ZHU H,SUN S H,et al.Experimental study of fluorescent magnetic nanoparticles labeled immunological assay for Vibrio parahaemolyticus thermostable direct heamolysin[J].Chin J Food Hygiene(中国食品卫生杂志),2017,29(3):302-306.
[8]ZOU Y H,QIU Y L,YU Y Y,et al.Study on gold immunochromatography assay for rapid detection of huperzine A[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,11(31):1370-1373.