黄粉虫资源化处理畜禽粪便的可行性研究
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摘要
为探索利用黄粉虫资源化处理畜禽粪便的可行性,研究了在黄粉虫幼虫的常规饲料中添加不同比例的猪粪对其生长发育和抗氧化酶系统(SOD,POD,CAT)的影响。结果表明:随着饲料中猪粪含量的增加,虫体的生物量增长率、对饲料的转化率和利用率均逐渐下降,含水率逐渐升高;可溶性蛋白含量总体上也呈逐渐降低的趋势;在饲喂添加猪粪饲料的初期(第5 d,第10 d时),黄粉虫幼虫体内的抗氧化酶活性升高,后期(第20 d时)又有所降低,但在猪粪添加量为10%时,虫体抗氧化酶的活性与空白组比差异均不显著。研究表明,利用黄粉虫处理畜禽粪便是可行的,为畜禽粪便的生物质资源化利用提供了参考。
In this paper, the larvae of yellow mealworm was fed by conventional feed which was mixed with definite proportions of swine manure, and explored the effects of mixed feed on its growth and the antioxidant enzyme system(SOD、POD、CAT), discussed the feasibility of using yellow mealworm to digest and utilize swine manure. The results showed that: with the increase of swine manure in the feed, the biomass growth rate, the conversion rate and utilization rate of the feed, and the content of soluble protein all decreased gradually, On the contrary, the moisture content increased; the activity of larvae's antioxidant enzymes improved at first and decreased later when fed by the mixed feed,but at the 10% of swine manure in the mixed feed, the activity of larvae's antioxidant enzymes showed no significant difference to the control group, which suggested that the addition of a little of swine manure in the conventional feed of yellow mealworm is feasible.
In this paper, the larvae of yellow mealworm was fed by conventional feed which was mixed with definite proportions of swine manure, and explored the effects of mixed feed on its growth and the antioxidant enzyme system(SOD、POD、CAT), discussed the feasibility of using yellow mealworm to digest and utilize swine manure. The results showed that: with the increase of swine manure in the feed, the biomass growth rate, the conversion rate and utilization rate of the feed, and the content of soluble protein all decreased gradually, On the contrary, the moisture content increased; the activity of larvae's antioxidant enzymes improved at first and decreased later when fed by the mixed feed,but at the 10% of swine manure in the mixed feed, the activity of larvae's antioxidant enzymes showed no significant difference to the control group, which suggested that the addition of a little of swine manure in the conventional feed of yellow mealworm is feasible.
引文
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[2]黄敬光,吕玉新,纪黔生,等.标准化规模养殖场发展现状,存在的问题及对策[J].畜禽业,2017(6):68-69.
[3]陶秀萍,董红敏.畜禽废弃物无害化处理与资源化利用技术研究进展[J].中国农业科技导报,2017,19(1):37-42.
[4]程文定,郜敏,吴天德,等.畜禽粪便等有机废弃物饲料资源化开发应用研究[J].中国畜牧兽医,2006,33(7):24-26.
[5]杭柏林,郑子勤,黄威.EM发酵畜禽粪便再生饲料技术[J].科学种养,2009(2):41.
[6]杨俊,邱美珍,段洪锋,等.牛粪生物饲料饲养番鸭试验及其最佳添加量的研究[J].饲料博览,2007(11):10-12.
[7]秦广军,张隐,霍宝怀.鸡粪再生饲料的制作与利用[J].现代畜牧兽医,2009(5):30.
[8]陈黎.猪粪资源化利用技术研究[D].杭州:浙江大学,2012.
[9]刘玉升,王付彬,崔俊霞,等.黄粉虫资源研究利用现状与进展[J].环境昆虫学报,32(1):106-114.
[10]高红莉,张硌,李洪涛,等.黄粉虫幼虫对城市污泥重金属的积累作用[J].中国生态农业学报,2011,19(1):150-154.
[11]孙国峰,戎安江,向钊.发酵鹌鹑粪便饲养黄粉虫的研究[J].畜禽业,2010(2):40-42.
[12]BRADFORD MM.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principal of protein-dye binding[J].Analytical biochemistry,1976(72):248-254.
[13]BEAUCHAMP C.Superoxide dismutase:Improved assays and applicable to acrylamide gels[J].Analytical biochemistry,1971,44(1):276-287.
[14]徐镜波,袁晓凡,郎佩珍.过氧化氢酶活性及活性抑制的紫外分光光度测定[J].环境化学,1997,16(1):73-76.
[15]SIMON LM,FATRAI Z,JONAS DE,etal.Study of peroxide metabolism enzymes during the development of phaseolus vulgris[J].Biochemie and physiologie der fplanzen,1974,166:388-392.
[16]Rowe C L,Hopkins W A,Zehnder C,et al.Metabolic costs incurred by crayfish(Procambarus acutus)in a trace element-polluted habitat:Further evidence of similar responses among diverse taxonomic groups[J].Comparative Biochemistry and Physiology D-Genomics&Proteomics,2001,129(3):275-283.
[17]Ribeiro S,Sousa J P,Nogueira A J,et al.Effect of endosulfan and parathion on energy reserves and physiological parameters of the terrestrial isopod Porcellio dilatatus[J].Ecotoxicology and Environmental Safety,2001,49(2):131-138.
[18]Papadimitriou E,Loumbourdis N S.Exposure of the frog Rana ridibunda to copper impact on two biomarkers,lipid peroxidation,and glutathione[J].Bulletin of Environment Contamination and Toxicology,2002,69(6):885-891.