邻苯二甲酸二丁酯污染对老鼠簕幼苗抗逆性生理特性的影响
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摘要
以红树植物老鼠簕作为实验材料,通过模拟海水实验,研究了不同质量浓度(0、1、2、5、10、20mg/L)的邻苯二甲酸二丁酯(DBP)污染暴露对老鼠簕幼苗叶绿素含量、比值及抗氧化酶系统(SOD,POD,CAT)等生理指标的影响。结果表明:(1)当DBP浓度为5mg/L时,DBP污染对老鼠簕幼苗叶绿素含量具有一定促进作用,其余浓度DBP污染均会使老鼠簕幼苗叶绿素含量显著下降; DBP污染对老鼠簕幼苗叶绿素a/b比值基本无影响;(2)随着DBP浓度升高,老鼠簕幼苗抗氧化酶活性均呈现先上升后下降的变化。说明DBP污染会导致老鼠簕幼苗叶片衰老;低浓度DBP暴露(≤10mg/L)可以诱导老鼠簕幼苗抗氧化酶系统活性增加,并保持一定时间细胞内活性氧平衡,高浓度DBP暴露(>10mg/L)会对老鼠簕幼苗的抗氧化酶系统产生不可逆的损伤。研究结果也为红树林湿地的保护,DBP的生态风险评价及污染控制提供了科学参考。
In the simulated pot experiments, the effects of different exposure of dibutyl phthalate(DBP) mass concentrations(0, 1, 2, 5, 10, 20 mg/L) on contents of chlorophyll, ratio of chlorophyll a/b, antioxidant enzymes(superoxide dismutase, peroxidase and catalase) et al were studied, taking the mangrove Acanthus ilicifolius L. seedlings as experimental materials. The results showed that the 5 mg/L DBP exposure would have a positive effect on chlorophyll content of the seedlings, while others reduced remarkably. The DBP exposure has hardly effect on the ratio of chlorophyll a/b in the seedlings. The results also showed the activity of antioxidant enzymes in Acanthus ilicifolius L. seedlings first increased then declined with the improve of DBP concentration. It is suggested that the DBP pollution could lead to leaf senescence. Antioxidant enzyme system could be induced to increase its activity with a low-concertation DBP exposure(≤10 mg/L) and maintain the metabolic balance of active oxygen in cells within a certain time, while could get irreversible damage with a high one(>10 mg/L). The conclusions of this study also provide a scientific reference for the protection of mangrove wetlands, as well as the ecological risk assessment and pollution control of DBP.
In the simulated pot experiments, the effects of different exposure of dibutyl phthalate(DBP) mass concentrations(0, 1, 2, 5, 10, 20 mg/L) on contents of chlorophyll, ratio of chlorophyll a/b, antioxidant enzymes(superoxide dismutase, peroxidase and catalase) et al were studied, taking the mangrove Acanthus ilicifolius L. seedlings as experimental materials. The results showed that the 5 mg/L DBP exposure would have a positive effect on chlorophyll content of the seedlings, while others reduced remarkably. The DBP exposure has hardly effect on the ratio of chlorophyll a/b in the seedlings. The results also showed the activity of antioxidant enzymes in Acanthus ilicifolius L. seedlings first increased then declined with the improve of DBP concentration. It is suggested that the DBP pollution could lead to leaf senescence. Antioxidant enzyme system could be induced to increase its activity with a low-concertation DBP exposure(≤10 mg/L) and maintain the metabolic balance of active oxygen in cells within a certain time, while could get irreversible damage with a high one(>10 mg/L). The conclusions of this study also provide a scientific reference for the protection of mangrove wetlands, as well as the ecological risk assessment and pollution control of DBP.
引文
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[16]杜娜.黄瓜对邻苯二甲酸二丁酯(DBP)的毒性响应[D].哈尔滨:东北农业大学,2014.Du Na.Toxicity Response of Cucumber to Dibutyl Phthalate[D].Haerbin:Northeast Agricultural University,2014.
[17]戚云.DBP、DEHP对小麦的毒理学效应研究[D].天津:天津工业大学,2016.Qi Yun.Toxicological Effects of DBP and DEHP on Wheat[D].Tianjin:Tianjin Polytechnic University,2016.
[18]曾晨,李元跃,陈融斌,等.DBP对秋茄幼苗光合作用和抗氧化酶活性的影响[J].集美大学学报(自然科学版),2014(4):253-258.Zeng Chen,Li Yuanyue,Chen Rongbin,et al.Physiological and biochemical properties of Kandelia candel seedlings under dibutyl phthalate(DBP)stress[J].Journal of Jimei University(Natural Science),2014(4):253-258.
[19]袁方,李鑫,余君萍,等.分光光度法测定叶绿素含量及其比值问题的探讨[J].植物生理学报,2009,45(1):63-66.Yuan Fang,Li Xin,Yu Junping,et al.Methodological Study on Determination of Chlorophyll Contents and Ratio of Chl a/Chl b by Spectrophotometry[J].Plant Physiology Journal,2009,45(1):63-66.
[20]Yang S,Jin W,Wang S,et al.Chlorophyll ratio analysis of the responses of algae communities to light intensity in spring and summer in Lake Erhai[J].Environmental Earth Sciences,2015,74(5):3877-3885.
[21]伍泽堂.超氧自由基与叶片衰老时叶绿素破坏的关系[J].植物生理学报,1991(4):277-279.Wu Zetang.Relationship between Superoxide Dismutase and Chlorophyll Degradation in Leaf Senescence[J].Plant Physiology Journal,1991(4):277-279.
[22]Zhang M,Tan F.Relationship Between Ratio of Chlorophyll a and b Under Water Stress and Drought Resistance of Different Sweet Potato Varieties[J].Seed,2001,18(11):12-16.
[23]Wang S,He Y,Ye S,et al.Effects of Di-(2-ethylhexyl)Phthalate(DEHP)on POD Activities and MDA Contents in Stem and Leaves of Vicia faba Seedlings[J].Asian Journal of Ecotoxicology,2010,5(4):587-591.
[24]Yin L,Huang J,Huang W,et al.Microcystin-RR-induced accumulation of reactive oxygen species and alteration of antioxidant systems in tobacco BY-2 cells[J].Toxicon Official Journal of the International Society on Toxinology,2005,46(5):507-512.
[25]杨舒贻,陈晓阳,惠文凯,等.逆境胁迫下植物抗氧化酶系统响应研究进展[J].福建农林大学学报(自然版),2016,45(5):481-489.Yang Shuyi,Chen Xiaoyang,Hui Wenkai,et al.Progress in responses of antioxidant enzyme systems in plant to environmental stresses[J].Journal of Fujian Agriculture and Forestry University(Natural Science Edition),2016,45(5):481-489.
[26]吴桂容.重金属Cd对桐花树幼苗生理生态效应及土壤酶的影响研究[D].厦门:厦门大学,2007.Wu Guirong.The Ecophysiological and Soil Enzymes Effects Research of heavy metal Cd on aegiceras corniculatum seedlings[D].Xiamen:Xiamen University,2007.
[27]万永吉,郑文教,方煜,等.重金属铬(Ⅲ)胁迫对红树植物秋茄幼苗SOD、POD活性及其同工酶的影响[J].厦门大学学报(自然科学版),2008(4):571-574.Wan Yongji,Zheng Wenjiao,Fang Yu,et al.Effects of Cr(III)Stress on ActiVities and Isozymes of SOD and POD of Kandelia candel Mangrove Seedlings[J].Journal of Xiamen University(Natural Science),2008(4):571-574.
[28]Demirevska-Kepova K,Simova-Stoilova L,Stoyanova Z,et al.Biochemical changes in barley plants after excessive supply of copper and manganese[J].Environmental&Experimental Botany,2004,52(3):253-266.
[29]吴桂容,严重玲.镉对桐花树幼苗生长及渗透调节的影响[J].生态环境,2006(5):1003-1008.Wu Guirong,Yan Chongling.Effects of Cd on the growth and osmotic adjustment regulation contents of Aegiceras conrniculatum seedlings[J].Ecology and Environment,2006(5):1003-1008.
[30]杨淑慎,高俊凤.活性氧、自由基与植物的衰老[J].西北植物学报,2001,21(2):36-41.Yang Shushen,Gao Junfeng.Influence of active oxygen and free radicals on plant senescence[J].Acta Botanica Boreali-Occidentalia Sinica,2001,21(2):36-41.
[31]林植芳,刘楠.活性氧调控植物生长发育的研究进展[J].植物学报,2012,47(1):74-86.Lin Zhifang,Liu Nan.Research Progress in the control and regulation of plant growth and development by reactive oxygen species[J].Chinese Bulletin of Botany,2012,47(1):74-86.
[32]赵胡,唐俊,郑文教.重金属Cu(2+)胁迫对红树植物秋茄幼苗生长及某些生理特性的影响[J].海洋科学,2016(4):65-72.Zhao Hu,Tang Jun,Zheng Wenjiao.Growth and physiological characteristics of Kandelia obovata seedlings under Cu2+stress[J].Marine Sciences,2016(4):65-72.
[2]van Wezel A P,Van V P,Posthumus R,et al.Environmental risk limits for two phthalates,with special emphasis on endocrine disruptive properties[J].Ecotoxicology&Environmental Safety,2000,46(3):305-321.
[3]Li K,Gu J.Biodegradation of di-n-butyl phthalate by mangrove microorganism Rhodococcus ruber 1K.[J].Chinese Journal of Applied Ecology,2005,16(8):1566-1568.
[4]Suttee A.Acanthus ilicifolius[J].Indian Journal of Pharmaceutical Sciences,2009,70(6):821-823.
[5]邓业成,骆海玉,张丽珍,等.14种红树植物对动物病原菌的抑菌活性[J].海洋科学,2012(3):37-41.Deng Yecheng,Luo Haiyu,Zhang Lizhen,et al.Inhibitory activity of fourteen mangrove plants against animal pathogens[J].Marine Sciences,2012(3):37-41.
[6]林鹏,林益明,杨志伟,等.中国海洋红树林药物的研究现状、民间利用及展望[J].海洋科学,2005(9):78-81.Lin Peng,Lin Yiming,Yang Zhiwei,et al.Reesarch status,civil utilization and ProsPect on marine mangrove drug in China-a review[J].Marine Sciences,2005(9):78-81.
[7]Lu Y,Lin M,Aitken R J.Exposure of spermatozoa to dibutyl phthalate induces abnormal embryonic development in a marine invertebrate Galeolaria caespitosa(Polychaeta:Serpulidae)[J].Aquatic Toxicology,2017,191.
[8]穆希岩,李成龙,黄瑛,等.两种邻苯二甲酸酯类污染物对斑马鱼胚胎发育的影响[J].中国环境科学,2017(9):3566-3575.Mu Xiyan,Li Chenglong,Huang Ying,et al.Effects of two phthalate esters on zebrafish embryos[J].China Environmental Science,2017(9):3566-3575.
[9]刘春晓,王平,李海燕,等.DBP对铜绿微囊藻生长和抗氧化酶的影响[J].环境科学与技术,2015,38(2):7-12.Liu Chunxiao,Wang Ping,Li Haiyan,et al.Effects of dibutyl phthalate on growth and enzymatic antioxidants of Microcytis aeruginosa[J].Environmental Science&Technology,2015,38(2):7-12.
[10]尹素真,李光德,刘明明,等.DBP和Cd复合污染对油菜的毒性作用研究[J].水土保持学报,2015(2):294-298.Yin Suzhen,Li Guangde,Liu Mingming,et al.Toxic effects of combined pollution DBP and Cd on rape[J].Journal of Soil and Water Conservation,2015(2):294-298.
[11]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2011:74-77,211-219.Gao Junfeng.Plant Physiology Experiment Guide[M].Beijing:Higher Education Press,2011:74-77,211-219.
[12]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:134-137.Li Hesheng.Plant physiology and biochemistry experiment principle and technology[M].Beijing:Higher Education Press,2000:134-137.
[13]中国科学院上海植物生理研究所.现代植物生理学实验指南[M].上海:科学出版社,1999:305-306.Science of Plant Physiology and Ecology,SIBS,CAS.Modern Plant Physiology Experiment Guide[M].Shanghai:Science Press,1999:305-306.
[14]杨兰芳,庞静,彭小兰,等.紫外分光光度法测定植物过氧化氢酶活性[J].现代农业科技,2009(20):364-366.Yang Lanfang,Pang Jing,Peng Xiaolan,et al.Measurement of catalase activity in plants by ultraviolet spectrophotometry[J].Modern Agricultural Science and Technology,2009(20):364-366.
[15]冯建灿,胡秀丽,毛训甲.叶绿素荧光动力学在研究植物逆境生理中的应用[J].经济林研究,2002(4):14-18.Feng Jiancan,Hu Xiuli,Mao Xunjia.Application of chlorophyll fluorescence dynamics to plant physiology in adverse circumstance[J].Economic Forest Researchers,2002(4):14-18.
[16]杜娜.黄瓜对邻苯二甲酸二丁酯(DBP)的毒性响应[D].哈尔滨:东北农业大学,2014.Du Na.Toxicity Response of Cucumber to Dibutyl Phthalate[D].Haerbin:Northeast Agricultural University,2014.
[17]戚云.DBP、DEHP对小麦的毒理学效应研究[D].天津:天津工业大学,2016.Qi Yun.Toxicological Effects of DBP and DEHP on Wheat[D].Tianjin:Tianjin Polytechnic University,2016.
[18]曾晨,李元跃,陈融斌,等.DBP对秋茄幼苗光合作用和抗氧化酶活性的影响[J].集美大学学报(自然科学版),2014(4):253-258.Zeng Chen,Li Yuanyue,Chen Rongbin,et al.Physiological and biochemical properties of Kandelia candel seedlings under dibutyl phthalate(DBP)stress[J].Journal of Jimei University(Natural Science),2014(4):253-258.
[19]袁方,李鑫,余君萍,等.分光光度法测定叶绿素含量及其比值问题的探讨[J].植物生理学报,2009,45(1):63-66.Yuan Fang,Li Xin,Yu Junping,et al.Methodological Study on Determination of Chlorophyll Contents and Ratio of Chl a/Chl b by Spectrophotometry[J].Plant Physiology Journal,2009,45(1):63-66.
[20]Yang S,Jin W,Wang S,et al.Chlorophyll ratio analysis of the responses of algae communities to light intensity in spring and summer in Lake Erhai[J].Environmental Earth Sciences,2015,74(5):3877-3885.
[21]伍泽堂.超氧自由基与叶片衰老时叶绿素破坏的关系[J].植物生理学报,1991(4):277-279.Wu Zetang.Relationship between Superoxide Dismutase and Chlorophyll Degradation in Leaf Senescence[J].Plant Physiology Journal,1991(4):277-279.
[22]Zhang M,Tan F.Relationship Between Ratio of Chlorophyll a and b Under Water Stress and Drought Resistance of Different Sweet Potato Varieties[J].Seed,2001,18(11):12-16.
[23]Wang S,He Y,Ye S,et al.Effects of Di-(2-ethylhexyl)Phthalate(DEHP)on POD Activities and MDA Contents in Stem and Leaves of Vicia faba Seedlings[J].Asian Journal of Ecotoxicology,2010,5(4):587-591.
[24]Yin L,Huang J,Huang W,et al.Microcystin-RR-induced accumulation of reactive oxygen species and alteration of antioxidant systems in tobacco BY-2 cells[J].Toxicon Official Journal of the International Society on Toxinology,2005,46(5):507-512.
[25]杨舒贻,陈晓阳,惠文凯,等.逆境胁迫下植物抗氧化酶系统响应研究进展[J].福建农林大学学报(自然版),2016,45(5):481-489.Yang Shuyi,Chen Xiaoyang,Hui Wenkai,et al.Progress in responses of antioxidant enzyme systems in plant to environmental stresses[J].Journal of Fujian Agriculture and Forestry University(Natural Science Edition),2016,45(5):481-489.
[26]吴桂容.重金属Cd对桐花树幼苗生理生态效应及土壤酶的影响研究[D].厦门:厦门大学,2007.Wu Guirong.The Ecophysiological and Soil Enzymes Effects Research of heavy metal Cd on aegiceras corniculatum seedlings[D].Xiamen:Xiamen University,2007.
[27]万永吉,郑文教,方煜,等.重金属铬(Ⅲ)胁迫对红树植物秋茄幼苗SOD、POD活性及其同工酶的影响[J].厦门大学学报(自然科学版),2008(4):571-574.Wan Yongji,Zheng Wenjiao,Fang Yu,et al.Effects of Cr(III)Stress on ActiVities and Isozymes of SOD and POD of Kandelia candel Mangrove Seedlings[J].Journal of Xiamen University(Natural Science),2008(4):571-574.
[28]Demirevska-Kepova K,Simova-Stoilova L,Stoyanova Z,et al.Biochemical changes in barley plants after excessive supply of copper and manganese[J].Environmental&Experimental Botany,2004,52(3):253-266.
[29]吴桂容,严重玲.镉对桐花树幼苗生长及渗透调节的影响[J].生态环境,2006(5):1003-1008.Wu Guirong,Yan Chongling.Effects of Cd on the growth and osmotic adjustment regulation contents of Aegiceras conrniculatum seedlings[J].Ecology and Environment,2006(5):1003-1008.
[30]杨淑慎,高俊凤.活性氧、自由基与植物的衰老[J].西北植物学报,2001,21(2):36-41.Yang Shushen,Gao Junfeng.Influence of active oxygen and free radicals on plant senescence[J].Acta Botanica Boreali-Occidentalia Sinica,2001,21(2):36-41.
[31]林植芳,刘楠.活性氧调控植物生长发育的研究进展[J].植物学报,2012,47(1):74-86.Lin Zhifang,Liu Nan.Research Progress in the control and regulation of plant growth and development by reactive oxygen species[J].Chinese Bulletin of Botany,2012,47(1):74-86.
[32]赵胡,唐俊,郑文教.重金属Cu(2+)胁迫对红树植物秋茄幼苗生长及某些生理特性的影响[J].海洋科学,2016(4):65-72.Zhao Hu,Tang Jun,Zheng Wenjiao.Growth and physiological characteristics of Kandelia obovata seedlings under Cu2+stress[J].Marine Sciences,2016(4):65-72.