美洲黑杨与山杨远缘杂交技术的研究
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摘要
用5种不同处理方法对克服辽宁杨×山杨(正交)和山杨×辽宁杨(反交)的杂交障碍进行研究,并用形态学标记法对杂种进行亲子鉴定。研究结果表明:正交杂种子代表现出偏母本型、中间型和偏父本型3种类型,而反交杂种子代表现出偏母本型和偏父本型两种类型,证明杂种子代是其真正的杂种,辽宁杨与山杨远缘杂交取得了成功,用正己烷熏蒸柱头后再用花粉提取液处理柱头是克服其正、反交杂交障碍的方法之一。
The technology of intersectional crossing between P. deltoides and P. davidiana was studied by five different treatments. And the hybrids and their parents were identified using morphological marker.The results showed that hybrids offspring of P. liaoningensis × P. davidiana were inclined to partial parent type,intermediate type and paternal parent type,while hybrids offspring of P. davidiana × P. liaoningensis were inclined to partial parent type and paternal parent type,which meant the hybrid progeny was true hybrids. The effect was better to break incompatible barriers by fumigating stigma with n-hexane followed by brushing stigma with the fluid extracted from pollen.
The technology of intersectional crossing between P. deltoides and P. davidiana was studied by five different treatments. And the hybrids and their parents were identified using morphological marker.The results showed that hybrids offspring of P. liaoningensis × P. davidiana were inclined to partial parent type,intermediate type and paternal parent type,while hybrids offspring of P. davidiana × P. liaoningensis were inclined to partial parent type and paternal parent type,which meant the hybrid progeny was true hybrids. The effect was better to break incompatible barriers by fumigating stigma with n-hexane followed by brushing stigma with the fluid extracted from pollen.
引文
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[2]李志新,戴福.杨树杂交育种研究进展[J].林业科技情报,2015,27(3):27~31.
[3]Willing R R,Pryor L D.Interspecific hybridization in poplar[J].Theoretical and Applied Genetics,1976(47):141~151.
[4]Knox R B,Willing R R,Ashford A E.Pollen wall proteins:role as recognition substances in interspecific incompatibility in poplars[J].Nature,1972,237:381~383.
[5]Guries R P,Stettler R F.Pre-fertilization barriers to hybridzation in the poplars[J].Silvae Genet,1976,25:37~44.
[6]斯塔罗娃H B.杨柳科育种[M].马常耕,译.北京:科学技术出版社,1984.
[7]Ronald W G.Intersectional hybridization of populus sections,leuce-aigeiros and leuce tacamahaca[J].Silvae Genet,1982,31:94~99.
[8]徐纬英.杨树[M].哈尔滨:黑龙江人民出版社,1988.
[9]张绮纹,苏晓华.克服杨树远缘杂交受精前障碍的研究[J].林业科学研究,1988(2):201~205.
[10]吴鸿锦,刘志光,韩克展,等.新杂交种沙毛杨的选育[J].北京林业大学学报,1996,18(3):48~53.
[11]张金凤,朱之悌,张志毅.黑白杨派间杂交试验研究[J].北京林业大学学报,1999,21(1):6~10.
[12]张金凤,朱之悌,张志毅等.中介亲本在黑白杨派间杂交中的应用[J].北京林业大学学报,2000,22(6):35~38.
[13]庞金宣,郑世锴,刘国兴等.窄冠型杨树新品种的选育[J].林业科学通讯,2001(4):8~9.
[14]高建社.黑杨与白杨远缘杂交技术的研究[D].西北农林科技大学,2005.
[15]葛颂,王海群,张灿明,等.八面山银杉林的遗传多样性与群体分化[J].植物学报,1997,39(3):266~271.
[16]彭儒胜,赵鑫闻,王胜东,等.美洲黑杨与胡杨杂交及其杂种子代亲子鉴定[J].辽宁林业科技,2013(5):1~4.
[17]杨成超.银白杨×白榆亲子鉴定及子代利用研究[D].中国林业科学研究院,2005.
[18]张妍,杨成超,冯连荣,等.杨树远缘杂交障碍克服及亲子鉴定技术研究进展[J].辽宁林业科技,2015(3):55~58.
[19]赵鑫闻.利用花粉管通道技术奖外源银白杨DNA导入黑杨[J].植物学报,2016,51(4):533~541.