三倍体欧洲山杨组织培养快繁技术研究

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英文篇名：Study on Technology of Tissue Culture and Rapid Propagation of Triploid Populus Tremula 作者：汪清锐 ; 吕林芳 英文作者：Wang Qingrui;Lv Linfang;Science and technology Service Center,Shanxi Province Poplar High-yield Forest Bureau of Sanggan River; 关键词：三倍体 ; 欧洲山杨 ; 组织培养 ; 增殖率 英文关键词：Triploid;;Populus tremula;;Tissue culture;;Multiplication rate 中文刊名：SXLK 英文刊名：Shanxi Forestry Science and Technology 机构：山西省桑干河杨树丰产林实验局科技服务中心; 出版日期：2018-06-15 出版单位：山西林业科技 年：2018 期：v.47;No.186 语种：中文; 页：SXLK201802011 页数：2 CN：02 ISSN：14-1098/S 分类号：30-31

摘要

笔者通过对比研究,建立了三倍体欧洲山杨组织培养系统。茎段外植体用0.1%Hg CI2或2.5%Na CIO溶液消毒后,接种到MS+0.5 mg/L 6-BA+0.05 mg/L NAA培养基上诱导芽。试管苗采用MS+1.0 mg/L 6-BA+0.5 mg/L KT+0.3 mg/L GA培养基进行增殖,增殖系数为15,生根采用MS+0.1 mg/L IBA培养基,生根率可达90%.
        Tissue culture system of triploid Populus tremula was set up through comparative study. The surface of stem explants was sterilized with 0. 1% Hg CI2 or 2. 5% Na CIO,and then the stem explants were inoculated on the culture medium which was MS + 0. 5 mg/L 6-BA + 0. 05 mg/L NAA to induce bud. Multiplication medium was MS + 1. 0 mg/L 6-BA +0. 5 mg/L KT + 0. 3 mg/L GA and the multiplication coefficient was 15. Rooting medium was MS + 0. 1 mg/L IBA and the rooting rate could reach 90%.

引文

[1]张彦文,刘丹梅.三倍体欧洲山杨组织培养再生系统的建立[J].丹东纺专学报,2002(3):33.
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    [3]郑洪.三倍体欧洲山杨快速繁殖体系的建立[D].北京:中国农业科学研究院,2006.