三桠乌药天然群体种子表型多样性研究
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摘要
为提高三桠乌药种质资源保护与利用成效,以河南、山东天然分布的7个三桠乌药群体的种子为材料,对其5个表型性状进行方差分析、相关分析及聚类分析。方差分析结果表明,三桠乌药种子表型性状在群体间和群体内均存在丰富变异,MP1天然群体种子的长度、宽度、体积和表面积与其它6个天然群体的相应性状均存在差异,且达到极显著水平;LS群体与MP1、MP2群体,MP1群体与其它6个群体的种子体积也均存在极显著差异。相关性分析结果表明,7个三桠乌药天然群体种子的5个表型性状与其采集地的海拔、经纬度、年均温和年降水量均存在一定相关性。聚类分析结果表明,在距离为2时,可以明显将7个群体分成4组:第一组包括MP1,第二组包括LS,第三组包括QX、MP2和MP3,第四组包括ZY和LC。昆嵛山MP1和崂山LS天然群体资源对整个三桠乌药种质资源的保护和创新利用具有重要价值。
To improve the efficiency of conservation and utilization of the germplasm resources of Lindera obtusiloba,the variance analysis,correlation analysis and cluster analysis were carried out on five phenotypic traits of the seeds of 7 L. obtusilobanatural populations distributed in Henan and Shandong provinces. The analysis of variance showed that there was abundant variation in the phenotypic traits of seeds between and within populations. The length,width,volume and surface area of MP1 seeds were extremely significantly different from those of the other six natural populations. The seed volume of LS was significantly different from that of MP1 and MP2,and that of MP1 was also significantly different from that of the other six natural populations.The correlation analysis showed that the five phenotypic traits of the seven natural populations of L. obtusiloba had certain correlations with altitude,latitude and longitude,annual mean temperature and annual precipitation. The cluster analysis showed that the seven populations could be divided into four groups at the distance value of 2: the first one included MP1,the second one included LS,the third one consisted of QX,MP2 and MP3,and the fourth one included ZY and LC. The natural populations of MP1 from Kunyu Mountain and LS from Laoshan Mountain have important values for conservation and innovation of L. obtusiloba germplasm resources.
To improve the efficiency of conservation and utilization of the germplasm resources of Lindera obtusiloba,the variance analysis,correlation analysis and cluster analysis were carried out on five phenotypic traits of the seeds of 7 L. obtusilobanatural populations distributed in Henan and Shandong provinces. The analysis of variance showed that there was abundant variation in the phenotypic traits of seeds between and within populations. The length,width,volume and surface area of MP1 seeds were extremely significantly different from those of the other six natural populations. The seed volume of LS was significantly different from that of MP1 and MP2,and that of MP1 was also significantly different from that of the other six natural populations.The correlation analysis showed that the five phenotypic traits of the seven natural populations of L. obtusiloba had certain correlations with altitude,latitude and longitude,annual mean temperature and annual precipitation. The cluster analysis showed that the seven populations could be divided into four groups at the distance value of 2: the first one included MP1,the second one included LS,the third one consisted of QX,MP2 and MP3,and the fourth one included ZY and LC. The natural populations of MP1 from Kunyu Mountain and LS from Laoshan Mountain have important values for conservation and innovation of L. obtusiloba germplasm resources.
引文
[1]李法曾,李文清,樊守金.山东木本植物志(上卷)[M].北京:科学出版社,2016:274.
[2]李文清,臧德奎,解孝满,等.山东珍稀濒危保护树种[M].北京:科学出版社,2016:61.
[3]臧德奎.山东珍稀濒危植物[M].北京:中国林业出版社,2017:166-167.
[4]Ye J W,Bai W N,Bao L,et al.Sharp genetic discontinuity in the aridity-sensitive Lindera obtusiloba(Lauraceae):solid evidence supporting the Tertiary floral subdivision in East Asia[J].Journal of Biogeography,2017,44(9):2082-2085.
[5]贺怡娴.5种山胡椒属植物的地理分布模拟及主导气候因子分析[D].杨凌:西北农林科技大学,2015.
[6]张勇杰.山东省三桠乌药种质资源调查与评价[D].泰安:山东农业大学,2014.
[7]陈云,王婷,李培坤,等.河南木札岭温带落叶阔叶林群落特征及主要乔木空间分布格局[J].植物生态学报,2016,40(11):1179-1188.
[8]藏亚静.宝天曼国家级自然保护区固定样地森林群落点格局分析[D].福州:福建农林大学,2016.
[9]张勇杰,梁婷婷,臧德奎,等.崂山三桠乌药群落组成和结构分析[J].农学学报,2014,4(4):60-63,74.
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[11]刘庆超,刘庆华,马宗骧,等.三桠乌药耐阴性研究[J].草业学报,2013,22(6):93-99.
[12]张勇杰,朱鸿菊,任莹,等.基于SRAP分子标记的三桠乌药遗传多样性分析[J].林业科技开发,2013,27(6):17-20.
[13]马宗骧,刘庆超,王奎玲,等.三桠乌药种子休眠及萌发特性[J].西北农业学报,2011,20(8):139-141.
[14]穆艳娟,刘丹,王磊,等.关于青岛市三桠乌药与法国冬青温室育苗发芽时间的研究[J].山东林业科技,2014,44(1):25-27.
[15]刘泽坤,陈海霞.三桠乌药叶片中挥发油成分及抑菌活性研究[J].中国实验方剂学杂志,2011,17(22):164-167.
[16]刘泽坤,陈海霞.三桠乌药树皮及茎枝中挥发油成分GCMS分析[J].安徽农业科学,2011,39(24):14639-14641.
[17]党东雨.三桠乌药的特性与其开发利用[J].北京林业大学学报,2001,23(增刊):134.
[18]彭强.肉桂混淆品——三桠乌药树皮的生药鉴定[J].中药材,1991,14(12):15-17.
[19]Choi H G,Choi Y H,Kim J H,et al.A new neolignan and lignans from the stems of Lindera obtusiloba Blume and their anti-allergic inflammatory effects[J].Archives of Pharmacal Research,2014,37(4):467-472.
[20]Lim D W,Lee M S,Her S,et al.Antidepressant-like effects of Lindera obtusiloba extracts on the immobility behavior of rats in the forced swim test[J].Molecules,2016,21(3):277-286.
[21]陈志成.不同条件下树木死亡的水力失衡和碳饥饿机制[D].北京:中国林业科学研究院,2016.
[22]周淑香,符亚茹,王超,等.西藏光核桃果实及种子表型性状变异研究[J].北方园艺,2013(23):38-40.
[23]张亚琦,王宁,赵越,等.青海高原西伯利亚白刺天然居群表型多样性研究[J].北方园艺,2013(9):64-67.
[24]魏海斌,朱春云,刘小利,等.青海核桃种质资源表型多样性研究[J].北方园艺,2015(12):20-23.
[25]谭好国,鲁仪增,刘立江,等.毒豆半同胞家系种子表型特征研究[J].山东林业科技,2016,46(5):59-61.
[2]李文清,臧德奎,解孝满,等.山东珍稀濒危保护树种[M].北京:科学出版社,2016:61.
[3]臧德奎.山东珍稀濒危植物[M].北京:中国林业出版社,2017:166-167.
[4]Ye J W,Bai W N,Bao L,et al.Sharp genetic discontinuity in the aridity-sensitive Lindera obtusiloba(Lauraceae):solid evidence supporting the Tertiary floral subdivision in East Asia[J].Journal of Biogeography,2017,44(9):2082-2085.
[5]贺怡娴.5种山胡椒属植物的地理分布模拟及主导气候因子分析[D].杨凌:西北农林科技大学,2015.
[6]张勇杰.山东省三桠乌药种质资源调查与评价[D].泰安:山东农业大学,2014.
[7]陈云,王婷,李培坤,等.河南木札岭温带落叶阔叶林群落特征及主要乔木空间分布格局[J].植物生态学报,2016,40(11):1179-1188.
[8]藏亚静.宝天曼国家级自然保护区固定样地森林群落点格局分析[D].福州:福建农林大学,2016.
[9]张勇杰,梁婷婷,臧德奎,等.崂山三桠乌药群落组成和结构分析[J].农学学报,2014,4(4):60-63,74.
[10]孟艳,赖雨,杨文嘉,等.江苏云台山三桠乌药群落研究[J].生物学杂志,2016,33(6):68-72.
[11]刘庆超,刘庆华,马宗骧,等.三桠乌药耐阴性研究[J].草业学报,2013,22(6):93-99.
[12]张勇杰,朱鸿菊,任莹,等.基于SRAP分子标记的三桠乌药遗传多样性分析[J].林业科技开发,2013,27(6):17-20.
[13]马宗骧,刘庆超,王奎玲,等.三桠乌药种子休眠及萌发特性[J].西北农业学报,2011,20(8):139-141.
[14]穆艳娟,刘丹,王磊,等.关于青岛市三桠乌药与法国冬青温室育苗发芽时间的研究[J].山东林业科技,2014,44(1):25-27.
[15]刘泽坤,陈海霞.三桠乌药叶片中挥发油成分及抑菌活性研究[J].中国实验方剂学杂志,2011,17(22):164-167.
[16]刘泽坤,陈海霞.三桠乌药树皮及茎枝中挥发油成分GCMS分析[J].安徽农业科学,2011,39(24):14639-14641.
[17]党东雨.三桠乌药的特性与其开发利用[J].北京林业大学学报,2001,23(增刊):134.
[18]彭强.肉桂混淆品——三桠乌药树皮的生药鉴定[J].中药材,1991,14(12):15-17.
[19]Choi H G,Choi Y H,Kim J H,et al.A new neolignan and lignans from the stems of Lindera obtusiloba Blume and their anti-allergic inflammatory effects[J].Archives of Pharmacal Research,2014,37(4):467-472.
[20]Lim D W,Lee M S,Her S,et al.Antidepressant-like effects of Lindera obtusiloba extracts on the immobility behavior of rats in the forced swim test[J].Molecules,2016,21(3):277-286.
[21]陈志成.不同条件下树木死亡的水力失衡和碳饥饿机制[D].北京:中国林业科学研究院,2016.
[22]周淑香,符亚茹,王超,等.西藏光核桃果实及种子表型性状变异研究[J].北方园艺,2013(23):38-40.
[23]张亚琦,王宁,赵越,等.青海高原西伯利亚白刺天然居群表型多样性研究[J].北方园艺,2013(9):64-67.
[24]魏海斌,朱春云,刘小利,等.青海核桃种质资源表型多样性研究[J].北方园艺,2015(12):20-23.
[25]谭好国,鲁仪增,刘立江,等.毒豆半同胞家系种子表型特征研究[J].山东林业科技,2016,46(5):59-61.