链霉菌Streptomyces sp.FJS31-2产卤化二型聚酮类化合物的发酵条件优化
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摘要
以产zunyimycin A及BE-24566B类卤化天然化合物的Streptomyces sp.FJS31-2为研究对象,分别利用高分辨质谱(HRMS)和高效液相色谱(HPLC)为检测手段,对不同的碳源、氮源组合的培养基在不同的培养时间和萃取条件下获得的目标化合物进行定性和产量的分析。在基础培养基内添加10 g/L无水乙醇浸泡24 h后的腐殖酸,培养17 d,采取乙酸乙酯萃取可以使目标化合物产量提高。
Taking Streptomyces sp.FJS31-2 which produces zunyimycin A and BE-24566 B halogenated natural compound as research object,the target compound obtained from the fermentation medium with different carbon and nitrogen sources at different culture time and extraction conditions were qualitatively and quantitatively analyzed by high resolution mass spectrum(HRMS) and high performance liquid chromatography(HPLC).Results showed when adding humic acid which soaked in the basic medium with 10 g/L absolute ethyl alcohol for 24 h,the target compound yield could be improved by ethyl acetate extraction at 17 d.
Taking Streptomyces sp.FJS31-2 which produces zunyimycin A and BE-24566 B halogenated natural compound as research object,the target compound obtained from the fermentation medium with different carbon and nitrogen sources at different culture time and extraction conditions were qualitatively and quantitatively analyzed by high resolution mass spectrum(HRMS) and high performance liquid chromatography(HPLC).Results showed when adding humic acid which soaked in the basic medium with 10 g/L absolute ethyl alcohol for 24 h,the target compound yield could be improved by ethyl acetate extraction at 17 d.
引文
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[15]LU Y,YUE C,SHAO M,et al.Molecular genetic characterization of an anthrabenzoxocinones gene cluster in Streptomyces sp.FJS31-2 for the biosynthesis of BE-24566B and zunyimycin ale[J].Molecules,2016,21(6):771-779.
[2]NEWMAN DJ,CRAGG GM,SNADER KM.Natural Products as sources of new drugs over the period 1981-2002[J].J Nat Prod,2003,66(7):1022-1037.
[3]TSUKAMOTO M,NAKAJIMA S,ARAKAWA H,et al.A new antitumor antibiotic BE-19412A produced by a Streptomcete[J].J Anantibiotics,1998,51(10):908-914.
[4]RENNER M K,JENSEN P K,FENICAL W.Neomangicols:structure and absolute stereochemistries of unprecedented halogenated sesterterpenes from a marine fungus of the gene Fusarium[J].Org Chem,1998,63(23):6346-6354.
[5]ODEH R A,AI-QAWASMEH R A,SINNOKROT M O,et al.Synthesis and qualitative structure activity relationship evaluations of quinoline-based bisarylimidazoles as antibacterial motifs[J].Med Chem,2016,12(6):563-573.
[6]岳昌武.卤化酶阳性链霉菌天然产物发现及生物合成基因簇鉴定[M].北京:金琅学术出版社,2016:14-21.
[7]王荫荫,邵美云,岳昌武.微生物来源卤化天然产物研究进展[J].遵义医学院学报,2016,39(4):439-444.
[8]李园园,保玉心,吕玉红,等.梵净山保护区土壤放线菌分离及其次级代谢产物初步研究[J].长江大学学报:自科版,2013(8):60-65.
[9]YUE C,NIU J,LIU N,et al.Cloning and identification of the lobophorin biosynthetic gene cluster from marine Streptomyces olivaceus strain FXJ7.023[J].Pakistan J Pharm Sci,2016,29(1):287-293.
[10]LIAO L,CHEN R,JIANG M,et al.Bioprospecting potential of halogenases from Arctic marine actinomycetes[J].BMC Microbiol,2016,16:34-43.
[11]YIN X,CHEN Y,ZHANG L,et al.Enduracidin analogues with altered halogenation patterns produced by genetically engineered strains of Streptomyces fungicidicus[J].J Nat Pro,2010,73(4):583-589.
[12]KOJIRI K,NAKAJIMA S,FUSA A,et al.BE-24566B,a new antibiotic produced by Streptomyces violaceusniger[J].J Antibiot,1995,48(12):1506-1508.
[13]田延军,张家祥,杨丽萍,等.黑曲霉SFGT601产葡萄糖酸钠发酵培养基及工艺条件的研究[J].中国酿造,2015,34(1):89-93.
[14]孙健,马吉胜,金瑾,等.黄连解毒汤各成分的HPLC-UV/MS定性与定量测定方法研究[J].药学学报,2006,41(4):380-384.
[15]LU Y,YUE C,SHAO M,et al.Molecular genetic characterization of an anthrabenzoxocinones gene cluster in Streptomyces sp.FJS31-2 for the biosynthesis of BE-24566B and zunyimycin ale[J].Molecules,2016,21(6):771-779.