宜春温泉水体与泉底沉积物细菌多样性分析
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摘要
研究宜春富硒温泉水体与泉底沉积物的细菌群落多样性。利用高通量测序技术分析泉水与沉积物中细菌群落结构与多样性。温泉水中主要的细菌类群为变形菌门和拟杆菌门,而在沉积物样品中的主要优势菌群为OP1、蓝细菌、浮霉菌门和绿弯菌门。细菌在属分类水平上,温泉水中优势菌群为不动杆菌属、假单胞菌属、水栖菌属、Thermosynechococcus、鞘脂杆菌属和金黄杆菌属等。沉积物样品细菌中优势菌群属于未知物种,在数据库中并没有相关的注释信息;其中已知的优势菌属为Candidatus acetothermum、Thermosynechococcus、亚热栖菌属、不动杆菌属。宜春温汤富硒温泉水体与沉积物中存在着丰富的微生物群落且组成差异性很大,该研究为了解与发掘温泉微生物菌种资源具有重要价值。
The purpose of this study was to investigate the bacterial community diversity of Yichun selenium-rich hot spring water body and its bottom sediment. The high throughput sequencing technology was used to analyze the bacterial community structure and diversity of hot spring water and its sediment. The main bacterial groups in hot spring water were Proteobacteria and Bacteroidetes. However, OP1, Cyanobacteria, Planctomycetes and Chloroflexi were the main dominant bacterial groups in sediment samples. On the level of genus classification, the dominant flora in hot spring water were Acinetobacter, Pseudomonas, Enhydrobacter, Thermosynechococcus, Sphingobacterium,and Chryseobacterium, etc. The dominant flora in spring sediment belonged to unknown species, and there was no relevant annotated information in the database. The known dominant genera were Candidatus acetothermum,Thermosynechococcus, Meiothermus and Acinetobacter. The microbial communities in selenium-rich hot spring water and its sediment in Wentang of Yichun were abundant, and the composition was very different. The study would be of great value for understanding and excavating microorganism strain resources in the hot spring.
The purpose of this study was to investigate the bacterial community diversity of Yichun selenium-rich hot spring water body and its bottom sediment. The high throughput sequencing technology was used to analyze the bacterial community structure and diversity of hot spring water and its sediment. The main bacterial groups in hot spring water were Proteobacteria and Bacteroidetes. However, OP1, Cyanobacteria, Planctomycetes and Chloroflexi were the main dominant bacterial groups in sediment samples. On the level of genus classification, the dominant flora in hot spring water were Acinetobacter, Pseudomonas, Enhydrobacter, Thermosynechococcus, Sphingobacterium,and Chryseobacterium, etc. The dominant flora in spring sediment belonged to unknown species, and there was no relevant annotated information in the database. The known dominant genera were Candidatus acetothermum,Thermosynechococcus, Meiothermus and Acinetobacter. The microbial communities in selenium-rich hot spring water and its sediment in Wentang of Yichun were abundant, and the composition was very different. The study would be of great value for understanding and excavating microorganism strain resources in the hot spring.
引文
Amin A.,Ahmed I.,Salam N.,Kim B.Y.,Singh D.,Zhi X.Y.,Xiao M.,and Li W.J.,2017,Diversity and distribution of Thermophilic bacteria in hot springs of Pakistan,Microbial Ecology,74(1):1-12
Angenent L.T.,Kelley S.T.,St A.A.,Amand A.S.,Pace N.R.,and Hernandez M.T.,2005,Molecular identification of potential pathogens in water and air of a hospital therapy pool,PNAS,USA,102(13):4860
Caporaso J.G.,Kuczynski J.,Stombaugh J.,Bittinger K.,Bushman F.D.,Costello E.K.,Fierer N.,Pe觡a A.G.,Goodrich J.K.,Gordon J.I.,Huttley G.A.,Kelley S.T.,Knights D.,Koenig J.E.,Ley R.E.,Lozupone C.A.,Mcdonald D.,Muegge B.D.,Pirrung M.,Reeder J.,Sevinsky J.R.,Turnbaugh P.J.,Walters W.A.,Widmann J.,Yatsunenko T.,Zaneveld J.,and Knight R.,2010,QIIME allows analysis of high-throughput community sequencing data,Nature Methods,7(5):335-336
Cheng Z.Y.,Li M.,Shi Y.,He P.,He L.L.,and Li F.S.,2015,Research of drought-response mechanism in sugarcane by high through-put sequencing-based digital gene expression profiling,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(9):2018-2028(程志远,李穆,石莹,何平,何丽莲,李富生,2015,利用高通量测序分析甘蔗的抗旱响应机制,分子植物育种,13(9):2018-2028)
Edgar R.C.,2013,UPARSE:highly accurate OTU sequences from microbial ampliconreads,Nature Methods,10(10):996
Irfan M.,Tayyab A.,Hasan F.,Khan S.,Badshah M.,and Shah A.A.,2017,Production and characterization of organic solvent-tolerant cellulase from Bacillus amylolique faciens AK9 Isolated from Hot Spring,Applied Biochemistry&Biotechnology,182(4):1390-1402
Khalaf M.A.,and Kochzius M.,2002,Community structure and biogeography of shore fishes in the Gulf of Aqaba,Red Sea,Helgoland Marine Research,55(4):252-284
Khalil A.,2011,Isolation and characterization of three thermophilic bacterial strains(lipase,cellulose and amylase producers)from hot springs in Saudi Arabia,African Journal of Biotechnology,10(44):8834-8839
Liao B.R.,Xiong X.J.,and Wu X.C.,2014,Analysis of effect of natural mineral water on average life expectancy of residents in Wentang Town,Jiangxi,Yichun Xueyuan Xuebao(Journal of Yichun College),36(12):62-64(廖兵荣,熊筱娟,伍晓春,2014,江西省温汤天然矿泉水对期望寿命的影响分析,宜春学院学报,36(12):62-64)
Liu H.T.,2016,The research on water resources of Selenium-rich hot spring and environmental protection in Wentang Town of Yichun,Nengyuan Yu Huanjing(Energy and Environment),(5):48-50(刘慧婷,宜春温汤富硒温泉水资源与环境保护研究,能源与环境,(5):48-50)
Magoc T.,and Salzberg S.L.,2011,FLASH:fast length adjustment of short reads to improve genome assemblies,Bioinformatics,27(21):2957
Mo Q.,Li T.Y.,Shen W.,Fan Y.,Chen X.Z.,Wang Z.X.,and Shi G.Y.,2014,Screening and identification of starch degradable bacteria from small hot spring and initial enzymatic property analysis of its amylase,Weishengwuxue Zazhi(Journal of Microbiology),34(1):28-32(莫芹,李腾云,沈微,樊游,陈献忠,王正祥,石贵阳,2014,一小型温泉中降解淀粉细菌的筛选、鉴定与淀粉酶性质初步分析,微生物学杂志,34(1):28-32)
Ruckmani A.,and Chakrabarti T.,2011,Analysis of bacterial community composition of a spring water from the Western Ghats,India using culture dependent and molecular approaches,Current Microbiology,62(1):7-15
Wang T.,Chai L.H.,Guo C.L.,Cui X.L.,and Xu L.H.,2003,Study on nine 16S r DNA closes came from two hot springs in the west Yunnan,Weishengwuxue Tongbao(Microbial China),30(5):38-42(王涛,柴丽红,郭春雷,崔晓龙,徐丽华,2003,滇西热泉中9个16S r DNA克隆的分析,微生物学通报,30(5):38-42)
Yang B.,and Chen X.H.,2007,Isolation and identification of thermophilic bacteria in hot springs of Xiamen coast,Yiny ong Haiyangxue Xuebao(Journal of Applied Oceanography),26(1):61-70(杨波,陈新华,2007,厦门地区近海温泉嗜热菌的分离、鉴定,应用海洋学学报,26(1):61-70)
Zhu H.D.,Chang F.,Luo J.C.,Hu C.,and Wang Z.Y.,2005,Screen of an Abundant-se-containing lactobacillus strain and its abundant-se-containing capability,Niangjiu Keji(Liquormaking Science&Technology),134(8):29-31(朱何东,常峰,罗俊成,胡承,王忠彦,2005,富硒乳酸菌的筛选及其富硒能力的初步研究,酿酒科技,134(8):29-31)
Angenent L.T.,Kelley S.T.,St A.A.,Amand A.S.,Pace N.R.,and Hernandez M.T.,2005,Molecular identification of potential pathogens in water and air of a hospital therapy pool,PNAS,USA,102(13):4860
Caporaso J.G.,Kuczynski J.,Stombaugh J.,Bittinger K.,Bushman F.D.,Costello E.K.,Fierer N.,Pe觡a A.G.,Goodrich J.K.,Gordon J.I.,Huttley G.A.,Kelley S.T.,Knights D.,Koenig J.E.,Ley R.E.,Lozupone C.A.,Mcdonald D.,Muegge B.D.,Pirrung M.,Reeder J.,Sevinsky J.R.,Turnbaugh P.J.,Walters W.A.,Widmann J.,Yatsunenko T.,Zaneveld J.,and Knight R.,2010,QIIME allows analysis of high-throughput community sequencing data,Nature Methods,7(5):335-336
Cheng Z.Y.,Li M.,Shi Y.,He P.,He L.L.,and Li F.S.,2015,Research of drought-response mechanism in sugarcane by high through-put sequencing-based digital gene expression profiling,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(9):2018-2028(程志远,李穆,石莹,何平,何丽莲,李富生,2015,利用高通量测序分析甘蔗的抗旱响应机制,分子植物育种,13(9):2018-2028)
Edgar R.C.,2013,UPARSE:highly accurate OTU sequences from microbial ampliconreads,Nature Methods,10(10):996
Irfan M.,Tayyab A.,Hasan F.,Khan S.,Badshah M.,and Shah A.A.,2017,Production and characterization of organic solvent-tolerant cellulase from Bacillus amylolique faciens AK9 Isolated from Hot Spring,Applied Biochemistry&Biotechnology,182(4):1390-1402
Khalaf M.A.,and Kochzius M.,2002,Community structure and biogeography of shore fishes in the Gulf of Aqaba,Red Sea,Helgoland Marine Research,55(4):252-284
Khalil A.,2011,Isolation and characterization of three thermophilic bacterial strains(lipase,cellulose and amylase producers)from hot springs in Saudi Arabia,African Journal of Biotechnology,10(44):8834-8839
Liao B.R.,Xiong X.J.,and Wu X.C.,2014,Analysis of effect of natural mineral water on average life expectancy of residents in Wentang Town,Jiangxi,Yichun Xueyuan Xuebao(Journal of Yichun College),36(12):62-64(廖兵荣,熊筱娟,伍晓春,2014,江西省温汤天然矿泉水对期望寿命的影响分析,宜春学院学报,36(12):62-64)
Liu H.T.,2016,The research on water resources of Selenium-rich hot spring and environmental protection in Wentang Town of Yichun,Nengyuan Yu Huanjing(Energy and Environment),(5):48-50(刘慧婷,宜春温汤富硒温泉水资源与环境保护研究,能源与环境,(5):48-50)
Magoc T.,and Salzberg S.L.,2011,FLASH:fast length adjustment of short reads to improve genome assemblies,Bioinformatics,27(21):2957
Mo Q.,Li T.Y.,Shen W.,Fan Y.,Chen X.Z.,Wang Z.X.,and Shi G.Y.,2014,Screening and identification of starch degradable bacteria from small hot spring and initial enzymatic property analysis of its amylase,Weishengwuxue Zazhi(Journal of Microbiology),34(1):28-32(莫芹,李腾云,沈微,樊游,陈献忠,王正祥,石贵阳,2014,一小型温泉中降解淀粉细菌的筛选、鉴定与淀粉酶性质初步分析,微生物学杂志,34(1):28-32)
Ruckmani A.,and Chakrabarti T.,2011,Analysis of bacterial community composition of a spring water from the Western Ghats,India using culture dependent and molecular approaches,Current Microbiology,62(1):7-15
Wang T.,Chai L.H.,Guo C.L.,Cui X.L.,and Xu L.H.,2003,Study on nine 16S r DNA closes came from two hot springs in the west Yunnan,Weishengwuxue Tongbao(Microbial China),30(5):38-42(王涛,柴丽红,郭春雷,崔晓龙,徐丽华,2003,滇西热泉中9个16S r DNA克隆的分析,微生物学通报,30(5):38-42)
Yang B.,and Chen X.H.,2007,Isolation and identification of thermophilic bacteria in hot springs of Xiamen coast,Yiny ong Haiyangxue Xuebao(Journal of Applied Oceanography),26(1):61-70(杨波,陈新华,2007,厦门地区近海温泉嗜热菌的分离、鉴定,应用海洋学学报,26(1):61-70)
Zhu H.D.,Chang F.,Luo J.C.,Hu C.,and Wang Z.Y.,2005,Screen of an Abundant-se-containing lactobacillus strain and its abundant-se-containing capability,Niangjiu Keji(Liquormaking Science&Technology),134(8):29-31(朱何东,常峰,罗俊成,胡承,王忠彦,2005,富硒乳酸菌的筛选及其富硒能力的初步研究,酿酒科技,134(8):29-31)
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