针刺嗅三针对帕金森病小鼠嗅球病理及黑质TH表达的影响
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摘要
目的观察"嗅三针"干预脂多糖(LPS)经鼻诱导的帕金森病小鼠行为学、嗅球病理改变以及酪氨酸羟化酶(TH)在黑质中的表达,阐明针刺"嗅三针"对帕金森病早期的干预效应是通过改善大鼠嗅觉障碍而发挥作用。方法取C57BL/6雄性小鼠40只,随机分为空白组(Sham),帕金森模型组(Model),帕金森模型+嗅三针干预组(XSZ),左旋多巴组(L-DOPA),每组10只。除空白组外,其余各组均用经鼻灌注LPS的方法建立帕金森模型,造模同时进行电针干预10 d,用HE染色法观察嗅球病理变化以及免疫组织化学法测定组织中TH的表达水平。结果嗅三针有保护帕金森模型嗅球病理改变的作用,也可以显著提高黑质TH的表达水平(P<0.05),与左旋多巴组疗效相当。结论 "嗅三针"干预可以改善LPS诱导的小鼠帕金森病行为学及病理学改变,其机制可能与降低TH在小鼠大脑黑质的表达有关。
Objective To observe the effect of Xiusanzhen on the behavior, pathological changes of olfactory bulb and the expression of tyrosine hydroxylase(TH) in substantia nigra induced by lipopolysaccharide(LPS) in mice with Parkinson's disease. The purpose of this study is to elucidate that the intervention effect of acupuncture Xiusanzhen on Parkinson's disease in the early stage may play a role by improving olfactory disorders in rats. Methods 40 C57 BL/6 male mice were randomly divided into blank group(Sham), Parkinson's model group(Model), Parkinson's model and Xiusanzhen intervention group(XSZ), L-DOPA group(10 rats in each group). In addition to the blank group, the other groups were established by nasal perfusion LPS model, modeling and electroacupuncture intervention for 10 days at the same time, the pathological changes of olfactory bulb and the expression of TH in tissues were observed by HE staining and immunohistochemistry. Results Xiusanzhen could protect the pathological changes of olfactory bulb in Parkinson's model and also significantly increase the expression of TH in substantia nigra(P < 0.05), which was equivalent to that in levodopa group. Conclusion Xiusanzhen intervention can improve the behavioral and pathological changes of Parkinson's disease induced by LPS in mice, and the mechanism might be related to the decrease of TH expression in the substantia nigra of mice brain.
Objective To observe the effect of Xiusanzhen on the behavior, pathological changes of olfactory bulb and the expression of tyrosine hydroxylase(TH) in substantia nigra induced by lipopolysaccharide(LPS) in mice with Parkinson's disease. The purpose of this study is to elucidate that the intervention effect of acupuncture Xiusanzhen on Parkinson's disease in the early stage may play a role by improving olfactory disorders in rats. Methods 40 C57 BL/6 male mice were randomly divided into blank group(Sham), Parkinson's model group(Model), Parkinson's model and Xiusanzhen intervention group(XSZ), L-DOPA group(10 rats in each group). In addition to the blank group, the other groups were established by nasal perfusion LPS model, modeling and electroacupuncture intervention for 10 days at the same time, the pathological changes of olfactory bulb and the expression of TH in tissues were observed by HE staining and immunohistochemistry. Results Xiusanzhen could protect the pathological changes of olfactory bulb in Parkinson's model and also significantly increase the expression of TH in substantia nigra(P < 0.05), which was equivalent to that in levodopa group. Conclusion Xiusanzhen intervention can improve the behavioral and pathological changes of Parkinson's disease induced by LPS in mice, and the mechanism might be related to the decrease of TH expression in the substantia nigra of mice brain.
引文
[1]陈梅玲,彭天婵.帕金森病的研究进展[J].华夏医学,2017,30(3):153-156.
[2]廉龙江,汪瀚,杨文明.帕金森病非运动症状中医药治疗研究进展[J].吉林中医药,2016,36(6):643-645.
[3]王慧新.帕金森病的中医治疗[J].吉林中医药,2015,35(8):791-793.
[4]DAUER W,PRZEDBORSKI S.Parkinson’s disease:mechanisms and models[J].Neuron,2003,39(6):889-909.
[5]GOETZ C G.The history of Parkinson’s disease:early clinical descriptions and neurological therapies[J].Cold Spring Harb Perspect Med,2011,1(1):a008862.
[6]陈泽颉,王瑞丹,张巍.帕金森病嗅觉障碍机制的研究进展[J].中国神经免疫学和神经病学杂志,2017,24(1):48-52.
[7]KHOO TK,YARNALL AJ,DUNCAN GW,et al.The spectrum of non-motor symptoms in early Parkinson’s disease[J].Neurology,2013,80(3):276-281.
[8]卞育婕,王莹雪,崔逸爽,等.脂多糖诱导帕金森病大鼠模型及行为学评估[J].华北理工大学学报(医学版),2017,19(6):426-429.
[9]葛波波,屈洪党,陈育华,等.脂多糖诱导的慢性帕金森病小鼠模型α-突触核蛋白的出现和转移变化[J].中国临床药理学与治疗学,2017,22(3):241-247.
[10]HE Q,YU W,WU J,et al.Intranasal LPS-mediated Parkinson’s model challenges the pathogenesis of nasal cavity and environmental toxins[J].PLo S One,2013,8(11):e78418.
[11]DUTTA G,ZHANG P,LIU B.The lipopolysaccharide Parkinson’s disease animal model:mechanistic studies and drug discovery[J].Fundam Clin Pharmacol,2008,22(5):453-464.
[12]张国柱,盛守权.帕金森病的治疗研究进展[J].基层医学论坛,2011,15(4):353-355.
[13]LIU M,BING G.Lipopolysaccharide animal models for Parkinson’s disease[J].Parkinsons Dis,2011,2011:327089.
[14]马骏,王华.电针帕金森模型大鼠对黑质TH含量的影响[J].针刺研究,2001,26(4):288-291.
[15]LI H,PARK G,BAEN,et al.Anti-apoptotic effect of modified Chun-simyeolda-tang,a traditional Koerean herbal formula,on MPTP-in-duce dneuronal cell death in a Parkinson’s disease mouse model[J].Ethnopharmacol,2015,24(176):336-344.
[16]NASKAR A,PRABHAKAR V,SINGHR,et al.Melatonin enhances LODPA therapeutic effects,helps to reduce its dose,and protects dopaminergic neuron sinl-methy1-4-pheny1-1,-2,3,6-tetrahy-dropyridine-induced Parkinsonism in mice[J].Pineal Res,2015,58(3):262-274.
[2]廉龙江,汪瀚,杨文明.帕金森病非运动症状中医药治疗研究进展[J].吉林中医药,2016,36(6):643-645.
[3]王慧新.帕金森病的中医治疗[J].吉林中医药,2015,35(8):791-793.
[4]DAUER W,PRZEDBORSKI S.Parkinson’s disease:mechanisms and models[J].Neuron,2003,39(6):889-909.
[5]GOETZ C G.The history of Parkinson’s disease:early clinical descriptions and neurological therapies[J].Cold Spring Harb Perspect Med,2011,1(1):a008862.
[6]陈泽颉,王瑞丹,张巍.帕金森病嗅觉障碍机制的研究进展[J].中国神经免疫学和神经病学杂志,2017,24(1):48-52.
[7]KHOO TK,YARNALL AJ,DUNCAN GW,et al.The spectrum of non-motor symptoms in early Parkinson’s disease[J].Neurology,2013,80(3):276-281.
[8]卞育婕,王莹雪,崔逸爽,等.脂多糖诱导帕金森病大鼠模型及行为学评估[J].华北理工大学学报(医学版),2017,19(6):426-429.
[9]葛波波,屈洪党,陈育华,等.脂多糖诱导的慢性帕金森病小鼠模型α-突触核蛋白的出现和转移变化[J].中国临床药理学与治疗学,2017,22(3):241-247.
[10]HE Q,YU W,WU J,et al.Intranasal LPS-mediated Parkinson’s model challenges the pathogenesis of nasal cavity and environmental toxins[J].PLo S One,2013,8(11):e78418.
[11]DUTTA G,ZHANG P,LIU B.The lipopolysaccharide Parkinson’s disease animal model:mechanistic studies and drug discovery[J].Fundam Clin Pharmacol,2008,22(5):453-464.
[12]张国柱,盛守权.帕金森病的治疗研究进展[J].基层医学论坛,2011,15(4):353-355.
[13]LIU M,BING G.Lipopolysaccharide animal models for Parkinson’s disease[J].Parkinsons Dis,2011,2011:327089.
[14]马骏,王华.电针帕金森模型大鼠对黑质TH含量的影响[J].针刺研究,2001,26(4):288-291.
[15]LI H,PARK G,BAEN,et al.Anti-apoptotic effect of modified Chun-simyeolda-tang,a traditional Koerean herbal formula,on MPTP-in-duce dneuronal cell death in a Parkinson’s disease mouse model[J].Ethnopharmacol,2015,24(176):336-344.
[16]NASKAR A,PRABHAKAR V,SINGHR,et al.Melatonin enhances LODPA therapeutic effects,helps to reduce its dose,and protects dopaminergic neuron sinl-methy1-4-pheny1-1,-2,3,6-tetrahy-dropyridine-induced Parkinsonism in mice[J].Pineal Res,2015,58(3):262-274.
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