倒心盾翅藤中的1个新鼠李糖苷
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摘要
目的研究傣药倒心盾翅藤Aspidopterys obcordate的化学成分。方法采用硅胶柱色谱、Sephadex LH-20柱色谱、ODS等色谱分离技术,对倒心盾翅藤茎醇提物的醋酸乙酯相和正丁醇相进行分离纯化,经现代波谱技术及物理常数测定等方法对所分离的化学成分进行结构鉴定。结果从倒心盾翅藤95%乙醇提取物中分离得到8个化合物,分别鉴定为3,4-二羟基苯甲酸-3-O-α-L-鼠李糖苷(1)、3,4-二羟基苯酚乙酸酯(2)、3,4-二羟基苯甲酸(3)、儿茶素(4)、β-谷甾醇(5)、胡萝卜苷(6)、cinnacasolide C(7)、尿囊素(8)。结论所有化合物均为首次从倒心盾翅藤中分离。其中,化合物1为新化合物,命名为倒心盾翅藤苷。
Objective To study the chemical constituents of Aspidopterys obcordate. Methods The chemical constituents from 95%EtOH extract of the stem of A. obcordate were isolated by repeated chromatograph with silica gel, Sephadex LH-20, and ODS. The structures were elucidated by spectroscopic analysis. Results Eight compounds were isolated and identified as 3,4-dihydroxybenzoic acid-3-O-α-L-rhamnoside(1), 3,4-dihydroxyphenyl acetate(2), 3,4-dihydroxybenzoic acid(3), catechin(4), β-sitosterol(5),daucosterol(6), cinnacasolide C(7), and alantan(8). Conclusion Compound 1 is a new compound, named as apobcoroside. All compounds are isolated from the plant of A. obcordate for the first time.
Objective To study the chemical constituents of Aspidopterys obcordate. Methods The chemical constituents from 95%EtOH extract of the stem of A. obcordate were isolated by repeated chromatograph with silica gel, Sephadex LH-20, and ODS. The structures were elucidated by spectroscopic analysis. Results Eight compounds were isolated and identified as 3,4-dihydroxybenzoic acid-3-O-α-L-rhamnoside(1), 3,4-dihydroxyphenyl acetate(2), 3,4-dihydroxybenzoic acid(3), catechin(4), β-sitosterol(5),daucosterol(6), cinnacasolide C(7), and alantan(8). Conclusion Compound 1 is a new compound, named as apobcoroside. All compounds are isolated from the plant of A. obcordate for the first time.
引文
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[2]李海涛,彭朝忠,管艳红,等.傣药倒心盾翅藤资源调查[J].时珍国医国药,2011,22(12):2999-3000.
[3]李晓花,牛迎凤,宋美芳,等.傣药倒心盾翅藤化学成分研究[J].亚太传统医药,2016,12(14):30-32.
[4]Hu M G,Li Y H,Sun Z C,et al.New polyoxypregnane glycosides from Aspidopterys obcordata vines with antitumor activity[J].Fitoterapia,2018,129:203-209.
[5]Bock K,Lundt I,Pedersen C.Assignment of anomeric structure to carbohydrates through geminal C-H coupling constants[J].Tetrahedron Lett,1973,14(13):1037-1040.
[6]Zhang C X,Zhang D M,Chen M F,et al.Antiproliferative triterpenoid saponins from the stem of Psychotria sp[J].Planta Med,2013,79(11):978-986.
[7]马娟,赵沛基,沈月毛,等.珍贵橙色束丝放线菌中新颖香草酰胺鼠李糖苷化合物[J].天然产物研究与开发,2006,18(5):721-722.
[8]Tsuda T,Watanabe M,Ohshima K,et al.Antioxidative components isolated from the seed of tamarind(Tamarindus indica L.)[J].J Agric Food Chem,1994,42(12):2671-2674.
[9]MiyazawaM,Oshima T,Tokura M,et al.Suppression of chemical mutagens-induced SOS response by phenolic acids from black rice bran using Salmonella typhimurium TA1535/p SK1002 umu test[J].J Oleo Sci,2003.52(9):471-481.
[10]Ana L Q,Galotta A,Maria A D,et al.Antioxidant and cytotoxic activities of‘A?Aí’(Euterpe precatoria Mart.)[J].Quim Nova,2008,31(6):1427-1430.
[11]刘兆花,王艳婷,钟惠民,等.长毛风车子的化学成分研究[J].天然产物研究与开发,2014,26(3):348-350.
[12]冯卫生.中药化学成分结构解析[M].北京:科学出版社,2008.
[13]Ngoc T M,Khoi N M,Ha D T,et al.Xanthine oxidase inhibitory activity of constituents of Cinnamomum cassia twigs[J].Bioorg Med Chem Lett,2015,43(46):4625-4628.
[14]陈路,刘远俊,黄菲菲,等.国产巴西人参醋酸乙酯部位化学成分研究[J].中草药,2018,49(6):1255-1260.