Pseudomonas sp.HS-2降解双酚F及脱氮的特性研究
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摘要
Pseudomonas sp.HS-2能以双酚F为唯一碳源、NH_4~+-N为唯一氮源进行生长,并通过异养硝化好氧反硝化过程脱氮。在35℃,150 r/min和pH=7.0条件下,考察了菌株HS-2降解双酚F的能力及其脱氮性能。结果表明,菌株HS-2能够降解500 mg/L双酚F。并且在最适碳氮比为20的条件下,双酚F和NH_4~+-N的去除率可分别达到99%和96%。并且发现氨单加氧酶参与了双酚F的降解。氮平衡分析表明,47.7%的初始NH_4~+-N转化为N_2。外加柠檬酸钠或硝态氮对双酚F和NH_4~+-N的去除有明显的促进作用。
Pseudomonas sp. HS-2 can grow by using bisphenol F as sole carbon source and NH_4~+-N as sole nitrogen source,and denitrify via the process of heterotrophic nitrification-aerobic denitrification(HN-AD). Bisphenol F degradation and denitrification capabilities of strain HS-2 have been investigated under the following conditions:35 ℃,150 r/min and pH 7.0. The results show that strain HS-2 could efficiently degrade 500 mg/L of bisphenol F. Under the optimal C/N ratio of 20,the removing rates of bisphenol F and NH_4~+-N can reach 99% and 96% in 48 h,respectively. In addition,it is found that during this process,ammonia monooxygenase has participated in BPF degradation.Nitrogen balance analysis demonstrates that 47.7% of initial NH_4~+-N is converted into N_2. The additional sodium citrate and NO_3~--N have remarkable facilitating effects on the removal of bisphenol F and NH_4~+-N.
Pseudomonas sp. HS-2 can grow by using bisphenol F as sole carbon source and NH_4~+-N as sole nitrogen source,and denitrify via the process of heterotrophic nitrification-aerobic denitrification(HN-AD). Bisphenol F degradation and denitrification capabilities of strain HS-2 have been investigated under the following conditions:35 ℃,150 r/min and pH 7.0. The results show that strain HS-2 could efficiently degrade 500 mg/L of bisphenol F. Under the optimal C/N ratio of 20,the removing rates of bisphenol F and NH_4~+-N can reach 99% and 96% in 48 h,respectively. In addition,it is found that during this process,ammonia monooxygenase has participated in BPF degradation.Nitrogen balance analysis demonstrates that 47.7% of initial NH_4~+-N is converted into N_2. The additional sodium citrate and NO_3~--N have remarkable facilitating effects on the removal of bisphenol F and NH_4~+-N.
引文
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[2]Bai Yaohui,Sun Qinghua,Zhao Cui,et al.Quinoline biodegradation and its nitrogen transformation pathway by a Pseudomonas sp.strain[J].Biodegradation,2010,21(3):335-344.
[3]Lu Hong,Weng Zihang,Wei Hao,et al.Simultaneous bisphenol F degradation,heterotrophic nitrification and aerobic denitrification by a bacterial consortium[J].Journal of Chemical Technology&Biotechnology,2016,92(4):854-860.
[4]Li Chune,Yang Jinshui,Wang Xin,et al.Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri,YG-24[J].Bioresource Technology,2015,182:18-25.
[5]Yao Shuo,Ni Jinren,Ma Tao,et al.Heterotrophic nitrification and aerobic denitrification at low temperature by a newly isolated bacterium,Acinetobacter sp.HA-2[J].Bioresource Technology,2013,139(13):80-86.
[6]Yang Lei,Ren Yongxiang,Liang Xian,et al.Nitrogen removal characteristics of a heterotrophic nitrifier Acinetobacter junii YB and its potential application for the treatment of high-strength nitrogenous wastewater[J].Bioresource Technology,2015,193:227-233.
[7]Ren Yongxiang,Yang Lei,Liang Xian.The characteristics of a novel heterotrophic nitrifying and aerobic denitrifying bacterium,Acinetobacter junii YB[J].Bioresource Technology,2014,171:1-9.
[8]黄廷林,白士远,张海涵,等.一株贫营养异养硝化-好氧反硝化细菌的分离鉴定及脱氮特性[J].环境工程学报,2015,9(12):5665-5671.
[9]魏复盛.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002:10-20.
[10]张庆军.纳氏试剂光度法测定水和废水中氨氮关键问题的研究[J].环境工程,2009,27(1):85-88.
[11]Cataldo D A,Maroon M,Schrader L E,et al.Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid 1[J].Communications in Soil Science and Plant Analysis,1975,6(1):71-80.
[12]Roh H,Subramanya N,Zhao F,et al.Biodegradation potential of wastewater micropollutants by ammonia-oxidizing bacteria[J].Chemosphere,2009,77(8):1084-1089.
[13]Ren L,Jia Y,Ruth N,et al.Biotransformations of bisphenols mediated by a novel Arthrobacter sp.strain YC-RL1[J].Applied Microbiology and Biotechnology,2016,100(4):1967-1976.
[14]万博,聂麦茜,齐慧霞,等.铜绿假单胞菌NY3好氧降解四溴双酚A的特性研究[J].安全与环境学报,2016(5):196-200.
[15]左薇.一株好氧反硝化菌的筛选鉴定及其脱氮特性分析[D].哈尔滨:哈尔滨工业大学,2006.
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