一种Off/On型荧光光度法检测鱼肉中氟含量
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摘要
聚乙烯吡咯烷酮(PVP)具有荧光特性,加入一定量的Fe~(3+)之后,由于Fe~(3+)和PVP发生反应导致体系荧光强度降低。而当在PVP-Fe~(3+)体系中加入少量F~-后,F~-与Fe~(3+)反应生成[FeF_6]~(3-)络合物导致体系荧光强度有所恢复,根据这种特性建立了一种Off/On型荧光光度法检测氟的新方法。在优化的实验条件下,荧光恢复程度与氟离子浓度在1. 5～30. 0μmol/L呈现良好的线性关系,检出限为0. 6μmol/L。应用该方法对鱼肉中氟离子含量测定,回收率在93. 33%～100. 00%。
Polyvinylpyrrolidone( PVP) owned the apparent fluorescence characteristics. In the presence of a small number of Fe~(3+),the interaction between Fe~(3+) and PVP could lead to an obvious decreasing of PVP fluorescence. However,when a small amount of F~- was added in Fe~(3+)-PVP system,the fluorescence intensity could partly been restored,which was ascribe to the formation of [FeF_6]~(3-) complexes. Thus,a new Off/On fluorescence spectrophotometry was established for the detection of fluoride. Under the optimized experimental conditions,the fluorescence recovery degree showed a good linear relationship with the fluorine ion concentration in the range of 1. 5-30. 0μmol/L. A detection limit as low as 0. 6 μmol/L was obtained. This method was applied to the detection of fluoride ion in fish meat with satisfactory results. The recovery rates were between 93. 33% and 100%.
Polyvinylpyrrolidone( PVP) owned the apparent fluorescence characteristics. In the presence of a small number of Fe~(3+),the interaction between Fe~(3+) and PVP could lead to an obvious decreasing of PVP fluorescence. However,when a small amount of F~- was added in Fe~(3+)-PVP system,the fluorescence intensity could partly been restored,which was ascribe to the formation of [FeF_6]~(3-) complexes. Thus,a new Off/On fluorescence spectrophotometry was established for the detection of fluoride. Under the optimized experimental conditions,the fluorescence recovery degree showed a good linear relationship with the fluorine ion concentration in the range of 1. 5-30. 0μmol/L. A detection limit as low as 0. 6 μmol/L was obtained. This method was applied to the detection of fluoride ion in fish meat with satisfactory results. The recovery rates were between 93. 33% and 100%.
引文
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[18] FENG Wen-hui,DONG Hui-xian,NIU Li-bo,et al. A novel Fe3O4@n Si O2@Ni Pd–PVP@m Si O2multi-shell core-shell nanocomposite for cinnamic acid hydrogenation in water[J]. J Mater Chem A,2015,3(39):19 807-19 814.
[19] BALAKUMAR VELLAICHAMY,PRAKASH PERIAKARUPPAN,THARMARAJ PAULMONY. Evaluation of a new biosensor based on in situ synthesized PPy-Ag-PVP nanohybrid for selective detection of dopamine[J]. The Journal of Physical. Chemistry B, 2017, 121(5):1 118-1 127.
[20] ASADPOUR-ZEYNALI,KARIM,MOLLARASOULI,et al. Novel electrochemical biosensor based on PVP capped Co Fe2O4@Cd Se core-shell nanoparticles modified electrode for ultra-trace level determination of rifampicin by square wave adsorptive stripping voltammetry[J]. Biosensors and Bioelectronics,2017,92:509-516.
[21]陈旭东,王新波.聚乙烯吡咯烷酮水溶液荧光光谱特性的研究[J].中山大学学报:自然科学版,2004,43(3):54-57.
[22] YU L,LIU Z,HU X,et al. Fluorescence quenching reaction of polyvinylpyrrolidone-eosin Y system for the etermination of polyvinylpyrrolidone.[J]. Journal of Fluorescence,2010,20(3):733-834.
[2]曹宗泽,向秋芬,冯琳,等.氟离子选择电极直接电位法测定四氟硼酸盐中游离的氟离子[J].理化检验-化学分册,2013,49(7):810-812.
[3]韩张雄,马娅妮,刘琦,等.微波消解-离子选择电极法测定植物样品中氟离子的实验条件优化[J].岩矿测试,2016,35(4):397-401.
[4]李志英,张晓阳.酸性铬兰K催化光度法测定水中的氟含量[J].化学研究与应用,2013,25(1):62-64.
[5] MIJIN YOO,SEOKAN PARKA,HAE-JO KIM. Activatable colorimetric and fluorogenic probe for fluoride detection by oxazoloindole-to-hydroxyethylindolium transformation[J]. RSC Advances,2016,6:19 910-19 915.
[6]任雪峰,安红钢,王小龙,等.响应面分析法优化抑制褪色光度法测定自来水中氟[J].工业水处理,2013,33(5):51-54.
[7]魏益华,张金艳,戴廷灿,等.离子色谱法测定地沟油和食用油中氯离子含量[J].食品科学,2011,32(12):213-215.
[8]姜新华,倪承珠,朱彬和,等.离子色谱-柱切换法测定土壤浸出液中的氟离子[J].色谱,2016,34(4):442-446.
[9]张宏亮,张吉范,梁汉贤,等.离子色谱法测定固体生物质燃料中氟的含量[J].理化检验-化学分册,2017,53(5):581-584.
[10]陈文,李东升,范长利,等.氟的单扫描极谱测定改进体系研究及分析应用[J].分析试验室,2009,28(5):11-15.
[11] BOSCHETTI W,DESSUY M B,PIZZATO A H,et al.New analytical method for total fluorine determination in soil samples using high-resolution continuum source graphite furnace molecular absorption spectrometry[J].Microchemical Journal,2017,130:276-280.
[12] HELOISA R CADORIM,JEFFERSON S DE GOIS,ALINE R BORGES,et al. Determination of fluorine in copper concentrate via high-resolution graphite furnace molecular absorption spectrometry and direct solid sample analysis-comparison of three target molecules[J]. Talanta,2018,176:178-186.
[13]申小爱,郭小峰,王红,等. Al(Ⅲ)-8-羟基喹啉-5-磺酸荧光猝灭法测定痕量氟离子[J].分析科学学报,2013,29(2):215-218.
[14] XIAOCHUN CHEN,SHAOMING YU,LIANG YANG,et al. Fluorescence and visual detection of fluoride ions using a photoluminescent graphene oxide paper sensor[J]. Nanoscale,2016,8:13 669-13 677.
[15] YANG Z R,WANG M M,WANG X S,et al. BoricAcid-Functional Lanthanide Metal-Organic Frameworks for Selective Ratiometric Fluorescence Detection of Fluoride Ions[J]. Analytical Chemistry,2017,89(3):1 930-1 936.
[16]姚宝慧,徐国财,张宏艳,等. PVP催化还原及稳定化纳米银的微波合成[J].无机化学学报,2010,26(9):1 629-1 632.
[17] GILLIAN COLLINS,MICHAEL SCHMIDT,GERARD P.MCGLACKEN,et al. Stability,oxidation,and shape evolution of PVP-Capped Pd nanocrystals[J]. The Journal of Physical. Chemistry C,2014,118(12):6 522-6 530.
[18] FENG Wen-hui,DONG Hui-xian,NIU Li-bo,et al. A novel Fe3O4@n Si O2@Ni Pd–PVP@m Si O2multi-shell core-shell nanocomposite for cinnamic acid hydrogenation in water[J]. J Mater Chem A,2015,3(39):19 807-19 814.
[19] BALAKUMAR VELLAICHAMY,PRAKASH PERIAKARUPPAN,THARMARAJ PAULMONY. Evaluation of a new biosensor based on in situ synthesized PPy-Ag-PVP nanohybrid for selective detection of dopamine[J]. The Journal of Physical. Chemistry B, 2017, 121(5):1 118-1 127.
[20] ASADPOUR-ZEYNALI,KARIM,MOLLARASOULI,et al. Novel electrochemical biosensor based on PVP capped Co Fe2O4@Cd Se core-shell nanoparticles modified electrode for ultra-trace level determination of rifampicin by square wave adsorptive stripping voltammetry[J]. Biosensors and Bioelectronics,2017,92:509-516.
[21]陈旭东,王新波.聚乙烯吡咯烷酮水溶液荧光光谱特性的研究[J].中山大学学报:自然科学版,2004,43(3):54-57.
[22] YU L,LIU Z,HU X,et al. Fluorescence quenching reaction of polyvinylpyrrolidone-eosin Y system for the etermination of polyvinylpyrrolidone.[J]. Journal of Fluorescence,2010,20(3):733-834.