SO_4~(2-)/TiO_2固体酸降解莲房中高聚体原花青素研究
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摘要
高聚体原花青素的抗氧化性能明显不如低聚体,而且不易被人体吸收利用.该研究致力于制备性能优良的SO_4~(2-)/TiO_2固体酸催化剂,采用该固体酸降解莲房中提取的高聚体原花青素.用紫外分光光度计和凝胶色谱仪进行分析,以平均聚合度和降解率作为参考指标,对该反应的降解效果进行评价.研究结果表明,以10 mL高聚体原花青素溶液作为降解原料,固体酸加入量为0.05 g,反应温度为70℃,反应时间为60 min的反应条件下,高聚体的平均聚合度从5.95降为2.31,降解率可以达到61.18%.
The antioxidant performance of the high-polymer procyanidins was not as good as that of oligomers, and it was not easily absorbed and utilized by the human body. This research focused on the preparation of SO_4~(2-)/TiO_2 solid acid catalysts with excellent properties. The solid acid was used to degrade the high-polymer procyanidins in lotus seed pot. The analysis was performed be using an ultraviolet spectrophotometer and a gel chromatograph. The average degree of polymerization and the degradation rate were used as reference indicators to evaluate the degradation effect of the reaction. The reaction condition was 10 mL high-polymer procyanidins solution as degradation raw material, solid acid addition amount of 0.05 g, reaction temperature of 70 ℃, and reaction time of 60 min. The results showed that the average polymerization degree of the high-polymer was decreased from 5.95 to 2.31 and the degradation rate could reach 61.18%.
The antioxidant performance of the high-polymer procyanidins was not as good as that of oligomers, and it was not easily absorbed and utilized by the human body. This research focused on the preparation of SO_4~(2-)/TiO_2 solid acid catalysts with excellent properties. The solid acid was used to degrade the high-polymer procyanidins in lotus seed pot. The analysis was performed be using an ultraviolet spectrophotometer and a gel chromatograph. The average degree of polymerization and the degradation rate were used as reference indicators to evaluate the degradation effect of the reaction. The reaction condition was 10 mL high-polymer procyanidins solution as degradation raw material, solid acid addition amount of 0.05 g, reaction temperature of 70 ℃, and reaction time of 60 min. The results showed that the average polymerization degree of the high-polymer was decreased from 5.95 to 2.31 and the degradation rate could reach 61.18%.
引文
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[5] 赵平,刘俊英,张月萍. 原花青素高聚体水解工艺[J]. 食品科技, 2011, 36(2): 174-178.
[6] 刘超英,杨晓瑞,束艳方,等. 固体酸降解木质纤维素的研究进展[J]. 食品工业科技, 2016, 37(7): 387-391.
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[11] 魏冠红,魏作君,苏宝根,等. 测定原花青素平均聚合度的一种新方法[J]. 中国食品学报, 2006, 6 (6): 112-116.
[2] 陈卫航,谭美亭,张婕. 响应曲面法优化莲房原花青素提取工艺研究[J]. 郑州大学学报(工学版), 2012, 33(2): 31-35.
[3] OU K Q, GU L W. Absorption and metabolism of proanthocyanidins[J]. Journal of functional foods, 2014, 7(1): 43-53.
[4] LUO L X, CUI Y, CHENG J H, et al. An approach for degradation of grape seed and skin proanthoc-yanidin polymers into oligomers by sulphu-rous acid[J]. Food chemistry, 2018, 256: 203-211.
[5] 赵平,刘俊英,张月萍. 原花青素高聚体水解工艺[J]. 食品科技, 2011, 36(2): 174-178.
[6] 刘超英,杨晓瑞,束艳方,等. 固体酸降解木质纤维素的研究进展[J]. 食品工业科技, 2016, 37(7): 387-391.
[7] 梁润娟. 离子液体环境下固体酸催化纤维素降解的研究[D]. 金华: 浙江师范大学物理化学研究所, 2013.
[8] LI B G. Extraction and purification of oligomeric proanthocyanidins from pine bark and its hypolipidemic effects[J]. Medicinal plant, 2011, 2(12): 4-6.
[9] 于荟,于清跃. SO42-/TiO2型固体酸催化剂的催化及稳定性能研究[J]. 硅酸盐通报, 2014, 33(3): 502-510.
[10] 郭慧. SO42-/TiO2固体超强酸催化剂的制备与改性和评价[D]. 哈尔滨: 哈尔滨工业大学应用化学系, 2006.
[11] 魏冠红,魏作君,苏宝根,等. 测定原花青素平均聚合度的一种新方法[J]. 中国食品学报, 2006, 6 (6): 112-116.