基于铜纳米粒子/氧化锌/石墨烯修饰电极的电化学方法测定硫酸卡那霉素
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摘要
在氧化锌/石墨烯(ZnO/GO)修饰ITO电极表面电沉积铜纳米粒子(CuNPs),制备了一种新型的电化学传感器,用于检测硫酸卡那霉素(KANA)。采用扫描电镜对制备的纳米材料以及修饰电极表面进行表征。优化后的测定条件为:在0.15 mol/L PBS缓冲溶液(pH 6.5)中,电沉积扫描圈数为40圈时, KANA在铜纳米粒子/氧化锌/石墨烯(CuNPs/ZnO/GO/ITO)电极上的电化学响应最大。KANA在电极表面的反应机理为单电子转移过程,修饰电极有效表面积为0.482 cm~2,是裸电极的2.42倍。在0.99～30.6μmol/L范围内,响应电流与KANA浓度呈良好的线性关系,线性方程为I_(pc)=-5.183c-4.544×10~(-6),R~2=0.9975,检出限为:0.31μmol/L,加标回收率为97.8%～103.6%。此传感器具有良好的稳定性与重现性,可用于药物中KANA的检测。
A novel electrochemical sensor was prepared by electrodeposition of copper nanoparticles(CuNPs) on a zinc oxide/graphene(ZnO/GO) modified ITO electrode and applied to the determination of kanamycin sulfate(KANA). The morphology of material and modified electrodes was characterized by scanning electron microscope. With optimization of measurement conditions systematically, the result showed that the electrochemical response of KANA on CuNPs/ZnO/GO/ITO electrode was the largest when the number of electrodeposition scanning circles were 40 in 0.15 mol/L PBS(pH=6.5) solution. The mechanism of electrode reaction of KANA involved one electron process, and the effective area(0.482 cm~2) of CuNPs/ZnO/GO/ITO electrode was 2.42 times as large as that of a bare ITO electrode. Moreover, the linear response range for determination of KANA was 0.99-30.6 μmol/L, the linear equation was I_(pc)=-5.183c-4.544×10~(-6) with the detection limit of 0.31 μmol/L, and the recoveries of standard addition experiments were 97.8%-103.6%. The sensor shows good stability and reproducibility, and can be used to detect kanamycin sulfate in the drug samples.
A novel electrochemical sensor was prepared by electrodeposition of copper nanoparticles(CuNPs) on a zinc oxide/graphene(ZnO/GO) modified ITO electrode and applied to the determination of kanamycin sulfate(KANA). The morphology of material and modified electrodes was characterized by scanning electron microscope. With optimization of measurement conditions systematically, the result showed that the electrochemical response of KANA on CuNPs/ZnO/GO/ITO electrode was the largest when the number of electrodeposition scanning circles were 40 in 0.15 mol/L PBS(pH=6.5) solution. The mechanism of electrode reaction of KANA involved one electron process, and the effective area(0.482 cm~2) of CuNPs/ZnO/GO/ITO electrode was 2.42 times as large as that of a bare ITO electrode. Moreover, the linear response range for determination of KANA was 0.99-30.6 μmol/L, the linear equation was I_(pc)=-5.183c-4.544×10~(-6) with the detection limit of 0.31 μmol/L, and the recoveries of standard addition experiments were 97.8%-103.6%. The sensor shows good stability and reproducibility, and can be used to detect kanamycin sulfate in the drug samples.
引文
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33 ZHANG Cui-Zhong,LIAN Huan,HUANG Hai-Feng,ZHANG Zhen-Fa,LIANG Cai-Yun,MENG Mei-Xiang,PENG Jin-Yun.Journal of Instrumental Analysis,2016,35(7):888-892张翠忠,连欢,黄海峰,张贞发,梁彩云,蒙美香,彭金云.分析测试学报,2016,35(7):888-892
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40 ZHANG Feng-Mei,WANG Jian.Chinese Journal of Antibiotics,2015,40(5):354-358张凤妹,王建.中国抗生素杂志,2015,40(5):354-358
41 Bai X J,Hou H,Zhang B L,Tang J L.Biosens.Bioelectron.,2014,56:112-116
2 ZHANG Zhi-Cun,YU Hong-Meng,LIU Quan.Acta Physiologica Sinica,2011,63(2):171-176张志存,余洪猛,刘全.生理学报,2011,63(2):171-176
3 Long Z,Guo Z,Acworth I N,Liu X D,Jin Y,Liu X G,Liu L Y,Liang L N.Talanta,2016,151:239-244
4 Xu Y Y,Han T,Li X Q,Sun L H,Zhang Y J,Zhang Y S.Anal.Chim.Acta,2015,891:298-303
5 Chen J,Li Z H,Ge J,Yang R,Zhang L,Qu L B,Wang H Q,Zhang L.Talanta,2015,139:226-232
6 Zhao M,Zhuo Y,Chai Y Q,Yuan R.Biomaterials,2015,52:476-483
7 Atul S,Georges I,Akhtar H,Ga?lle C,Sunil B,Jean L M.Sens.Actuators B,2017,245:507-515
8 Liu R,Yang Z H,Guo Q,Zhao J C,Ma J,Kang Q,Tang Y F,Xue Y,Lou X H,He M.Electrochim.Acta,2015,182:516-523
9 GB/T 1316-2015,Kanamycin Sulfate.Pharmacopoeia of the People's Repubic of China.硫酸卡那霉素.中国人民共和国药典.GB/T 1316- 2015
10 LI Min,HOU Jin-Feng,LU Yan,LI Wen-Dong,WANG Jun-Qiu,CHE Bao-Quan.Chinese Journal of Antibiotics,2018,43(3):341-346李珉,侯金凤,陆岩,李文东,王俊秋,车宝泉.中国抗生素杂志,2018,43(3):341-346
11 Han S,Li B Q,Song Z,Pan S H,Zhang Z C,Yao H,Zhu S Y,Xu G B.Analyst,2017,142:218-223
12 Song H Y,Kang T F,Li N N,Lu L P,Cheng S Y.Anal.Methods,2016,8:3366-3371
13 Yuan G H,Wang G,Wang H,Bai J T.Ionics,2015,21:365-371
14 Geim A K,Novoselov K S.Nat.Mater.,2007,6:183-191
15 HUO Ran,WU Yu-Xuan,YANG Yu,PIAO Shu-Qing,ZHANG Zhi-Cheng,XIAO Ji-Hai,SHI Ling.Chinese J.Appl.Chem.,2019,36(3):245-258霍冉,吴雨萱,杨煜,朴树清,张治城,肖佶海,史翎.应用化学,2019,36(3):245-258
16 Wu Z S,Zhou G M,Yin L C,Ren W C,Li F,Cheng H M.Nano Energy,2012,1(1):107-131
17 Hsieh C T,Lin C Y,Chen Y F,Lin J S.Electrochim.Acta,2013,111:359-365
18 Kim H,Park J H,Suh M,Ahn J R,Ju S.Appl.Phys.Lett.,2012,100(6):063112-063115
19 FAN Yong-Mei,ZHANG Ji-Mei,MI Chao,CAI Yu-Ling,ZHANG Li-Ping,ZHU Hai-Bin.Chinese J.Anal.Chem.,2018,46(1):33-38范咏梅,张纪梅,米超,蔡宇玲,张丽萍,朱海彬.分析化学,2018,46(1):33-38
20 ZUO Yin-Ze,CHEN Liang,ZHU Bin,GAO Yan-Min.Journal of Materials Engineering,2018,46(5):22-28左银泽,陈亮,朱斌,高延敏.材料工程,2018,46(5):22-28
21 Li Y Z,Cao Y L,Xie J,Jia D Z,Qin H Y,Liang Z T.Catal.Commun.,2015,58:21-25
22 Zhang M M,Lu X,Wang H Y,Liu X L,Qin Y J,Zhang P,Guo Z X.RSC Adv.,2016,6(42):35945-35951
23 Deiminiat B,Razavipanah I,Rounaghi G H,Arbab-Zavar M H.Sens.Actuators B,2017,244:785-795
24 Gusm?o R,Loípez-Puente V,Yate L,Pastoriza-Santos I,Pérez-Juste J,González-Romero E.Mater.Today Commun.,2017,11:11-17
25 Guo X N,Hao C H,Jin G Q,Zhu H Y,Guo X Y.Angew.Chem.Int.Edit.,2014,53(7):1973-1977
26 ZHANG Cui-Zhong,ZHANG Zhen-Fa,LIAN Huan,LIANG Cai-Yun,LI Kai,PENG Jin-Yun.Chinese J.Anal.Chem.,2017,45(1):28-34张翠忠,张贞发,连欢,梁彩云,李凯,彭金云.分析化学,2017,45(1):28-34
27 Marlinda A R,Huang N M,Muhamad M R,Anamt M N,Chang B Y S,Yusoff N,Harrison I,Lim H N,Chia C H,Kumar S V.Mater.Lett.,2012,80(1):9-12
28 ZHANG Li-Feng,ZHANG Jin-Zhen,SONG Qiao-Lan,LIU Yi,GUO Shou-Wu.Journal of Synthetic Crystals,2014,43(12):3235-3240张利锋,张金振 ,宋巧兰,刘毅,郭守武.人工晶体学报,2014,43(12):3235-3240
29 Qiang X L,Xia J F,Wang Z H,Xia Y Z,Zhang F F,Li Y H.Adv.Mater.Res.,2012,600:238-241
30 Saby C,Ortiz B,Champagne G Y,Bélanger D.Langmuir,1997,13(25):6805-6813
31 ZHANG Cui-Zhong,ZHANG Zhen-Fa,LIAN Huan,LIANG Cai-Yun,LI Kai,PENG Jin-Yun.Journal of Instrumental Analysis,2017,36(2):247-251张翠忠,张贞发,连欢,梁彩云,李凯,彭金云.分析测试学报,2017,36(2):247-251
32 Guo X N,Hao C H,Jin G Q,Zhu H Y,Guo X Y.Angew.Chem.Int.Edit.,2014,53(7):1973-1977.
33 ZHANG Cui-Zhong,LIAN Huan,HUANG Hai-Feng,ZHANG Zhen-Fa,LIANG Cai-Yun,MENG Mei-Xiang,PENG Jin-Yun.Journal of Instrumental Analysis,2016,35(7):888-892张翠忠,连欢,黄海峰,张贞发,梁彩云,蒙美香,彭金云.分析测试学报,2016,35(7):888-892
34 Yin H J,Tang H J,Wang D,Gao Y,Tang Z Y.ACS Nano,2012,6(9):8288-8297
35 Anson F C.Anal.Chem.,1964,36(4):932-934
36 Wang S F,Xu Q.Bioelectrochemistry,2007,70:296-300
37 Zhang X,Zhang Y C,Ma L X.Sens.Actuators B,2016,227(11):488-496
38 Laviron E J.Electroanal.Chem.,1979,101(1):19-28
39 ZHANG Xue-Jiao,LIU Chun-Ye,YANG Li-Yan,SONG Jin-Gui,ZHAO Xiang-Xin.Physical Testing and Chemical Analysis Part B:Chemical Analysis,2016,52(5):510-513张雪娇,刘春叶,杨黎燕,宋金贵,赵相欣.理化检验-化学分册,2016,52(5):510-513
40 ZHANG Feng-Mei,WANG Jian.Chinese Journal of Antibiotics,2015,40(5):354-358张凤妹,王建.中国抗生素杂志,2015,40(5):354-358
41 Bai X J,Hou H,Zhang B L,Tang J L.Biosens.Bioelectron.,2014,56:112-116