汉防己甲素对人神经母细胞瘤SH-SY5Y增殖和凋亡的影响
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摘要
目的探讨汉防己甲素对神经母细胞瘤SH-SY5Y细胞株的增殖抑制和诱导凋亡的作用。方法通过CCK-8实验检测汉防己甲素对于细胞生长的抑制率,流式细胞术检测Tet作用于细胞后,对细胞活性氧、线粒体膜电位、凋亡和周期的影响。结果汉防己甲素能显著抑制SH-SY5Y细胞生长,基本呈时间-剂量依赖性。流式细胞术检测显示不同浓度汉防己甲素处理后,活性氧明显升高,线粒体膜电位明显下降,细胞凋亡比例明显升高,G_0/G_1期细胞占比明显增高。结论汉防己甲素能够抑制SH-SY5Y细胞增殖并且诱导其发生凋亡。
Objective To investigate the effects of Tetrandrine on the proliferation and apotosis of human SH-SY5 Y cells.Methods CCK-8 assay was used for the cell viability test. Flow cytometry was used to determine the intracellular reactive oxygen species(ROS), mitochondrial membrane potential(JC-1), apoptotic ratio and cell cycle after Tetrandrine treatment. The cells with sham treatment served as the control. Results Tetrandrine significantly inhibited the proliferation of SH-SY5 Y cells in a time-and dose-dependent manner. The cell apoptosis and the production of intracellular ROS also increased remarkably by Tetrandrine treatment while JC-1 decreased. Cell cycle analysis further revealed that the percentage of cells in G_0/G_1 phase increased, in contrasting a statistical decrease of cell in G_2/M phase. Conclusion Tetrandrine could inhibit the proliferation and induce the apoptosis of SH-SY5 Y cells by blocking cell cycle in G_0/G_1 phase.
Objective To investigate the effects of Tetrandrine on the proliferation and apotosis of human SH-SY5 Y cells.Methods CCK-8 assay was used for the cell viability test. Flow cytometry was used to determine the intracellular reactive oxygen species(ROS), mitochondrial membrane potential(JC-1), apoptotic ratio and cell cycle after Tetrandrine treatment. The cells with sham treatment served as the control. Results Tetrandrine significantly inhibited the proliferation of SH-SY5 Y cells in a time-and dose-dependent manner. The cell apoptosis and the production of intracellular ROS also increased remarkably by Tetrandrine treatment while JC-1 decreased. Cell cycle analysis further revealed that the percentage of cells in G_0/G_1 phase increased, in contrasting a statistical decrease of cell in G_2/M phase. Conclusion Tetrandrine could inhibit the proliferation and induce the apoptosis of SH-SY5 Y cells by blocking cell cycle in G_0/G_1 phase.
引文
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13 Li W, Yang C, Lu J, et al. Tetrandrine protects mouse retinal ganglion cells from ischemic injury[J]. Drug Des Devel Ther, 2014,21(8):327-339
2 Bai CH, Jian LG, Bing QZ,et al. Tetrandrine inhibits Wnt/β-catenin signaling and suppresses tumor growth of human colorectal cancer[J]. Mol Pharmacol, 2011, 79(2):211-219
3 Rong Q, Shen HL, Cao Y, et al. Tetrandrine induces mitochondriamediatedapoptosis in human gastric cancer BGC-823 cell[J]. PLoS One, 2013, 8(10):1-10
4 Li X,Su B, Liu R,et al. Tetrandrine induces apoptosis and triggers caspase cascade in human bladder cancer cells[J]. J Surg Res,2011,166(1):e45-51
5 Wei X, Bisrat GD, Lara L, et al. Tetrandrine, a compound common in Chinese traditional medicine, preferentially kills breast cancer tumor initiating cells(TICs)in vitro[J]. Cancers:Basel, 2011,3(2):2274-2285
6 Bhagya N, Chandrashekar KR. Tetrandrine and cancer-An overview on the molecular approach[J]. Biomed Pharmacother, 2018, 97(116):624-632
7 Yagi Y, Fushida S, Harada S, et al. Effects of valproic acid on the cell cycle and apoptosis through acetylation of histone and tubulin in a scirrhous gastric cancer cell line[J]. J Exp Clin Cancer Res,2010,29(1):149
8 Sun X, Xu R, Deng Y, et al. Effects of tetrandrine on apoptosis and radiosensitivity of nasopharyngeal carcinoma cell line CNE[J]. Acta Biochim Biophys Sin:Shanghai, 2007, 39(11):869-878
9 Lee JH, Kang GH, Kim KC, et al. Tetrandrine induced cell cycle arrest and apoptosis in A 549 human lung carcinoma cells[J]. Int J Oncol, 2002, 21(6):1239-1244
10 Kuo PL, Lin CC. Tetrandrine induced cell cycle arrest and apoptosis in Hep G_2 cells[J]. Life Sci, 2003,73(2):243-252
11 Zhang Z, Liu T, Yu M, et al. The plant alkaloid tetrandrine inhibits metastasis via autophagy-dependent Wnt/β-catenin and metastatic tumor antigen 1 signaling in human liver cancer cells[J]. J Exp Clin Cancer Res,2018,37(1):7
12 Dang Y, Xu Y, Wu W, et al. Tetrandrine suppresses lipopolysaccharide-induced microglial activation by inhibiting NF-κB and ERK signaling pathways in BV2 cells[J]. PLoS One, 2014, 9(8):e102522
13 Li W, Yang C, Lu J, et al. Tetrandrine protects mouse retinal ganglion cells from ischemic injury[J]. Drug Des Devel Ther, 2014,21(8):327-339