新疆地方绵羊TLR9基因多态性分析
|
摘要
通过分析TLR9基因的多态性,探究新疆不同绵羊品种抗病力的分子机制。以塔什库尔干羊、和田羊和多浪羊共107个样本为研究对象,采用PCR、测序法和生物信息学方法分析TLR9基因的SNP及氨基酸差异。发现7个SNP,其中3个位点为同义突变,4个位点为非同义突变,确定为12个等位基因,其中新发现6个等位基因。首次发现氨基酸G437R的突变。6个等位基因对应的TLR9蛋白3-D结构存在差异,可能影响机体对PAMP的识别。绵羊TLR9基因外显子2具有较高的多态性,导致LRR空间结构改变,进而影响机体对疾病的感受性。本研究为新疆南疆地区绵羊抗病育种提供基础数据。
The resistance molecular mechanism of different sheep in Xinjiang was studied by analyzing the polymorphism of TLR9 gene. The SNP and amino acid differences of TLR9 genes were analyzed by PCR, sequencing and bioinformatics in tested 107 samples of Tashkurgan, Hetian and Duolang sheep. 7 SNPS were found that included three of synonymous mutations, and four loci of non-synonymous mutations, which were identified as 12 alleles, and the six alleles were newly found. The mutation of amino acid G437 R was the first discovered. There are differences in the 3-Dimensiona structure of TLR9 protein corresponding to 6 alleles, which might affect the identification of(pathogen associated molecular pattern, PAMP) PAMP. The exon 2 of sheep TLR9 gene has high polymorphism,which results in the change of(leucine rich repeats, LRR) LRR space structure, thus affecting the organism's susceptibility to disease. The study provided basic data for disease breeding of sheep in southern area of Xinjiang.
The resistance molecular mechanism of different sheep in Xinjiang was studied by analyzing the polymorphism of TLR9 gene. The SNP and amino acid differences of TLR9 genes were analyzed by PCR, sequencing and bioinformatics in tested 107 samples of Tashkurgan, Hetian and Duolang sheep. 7 SNPS were found that included three of synonymous mutations, and four loci of non-synonymous mutations, which were identified as 12 alleles, and the six alleles were newly found. The mutation of amino acid G437 R was the first discovered. There are differences in the 3-Dimensiona structure of TLR9 protein corresponding to 6 alleles, which might affect the identification of(pathogen associated molecular pattern, PAMP) PAMP. The exon 2 of sheep TLR9 gene has high polymorphism,which results in the change of(leucine rich repeats, LRR) LRR space structure, thus affecting the organism's susceptibility to disease. The study provided basic data for disease breeding of sheep in southern area of Xinjiang.
引文
Du Y.,Fang X.T.,Chen H.,Zhang C.L.,and Shi X.Y.,2009,The development of TLR9 genetic polymorphism and disease relationship,Keji Xinxi(Science and Technology Information)(9):31-32(杜毓,房兴堂,陈宏,张春雷,石秀英,2009,TLR9基因遗传多态性与疾病关系的研究进展,科技信息,(9):31-32)
Kotze A.,and Muller G.H.,1994,Genetic relationship in South African cattle breeds.In:Proceedings of the 5th world congress on genetics applied to livestock production,Guelph Canada,University of Guelph,Guelph,Ontario,Canada,21413-416
Lee C.Y.,Qin J.,Munyard K.A.,Subramaniam N.S.,Wetherall JD.,Stear M.J.and Groth D.M.,2012,Conserved haplotype blocks within the sheep MHC and low SNP heterozygosity in the Class IIa subregion,Anim.Genet.,43(3):429-437
Li G.L.,Mu S.M.,Li Z.Q.,and Kang X.J.,2006,TLR-specific receptors in natural immunity,Shengming De Huaxue(Chemistry of Life),26(6):495-497(李根亮,穆淑梅,李彦芹,康现江,2006,TLR-天然免疫中的特异性受体,生命的化学,26(6):495-497)
Lu B.S.,and Huang P.T.,1996,The study of the four-fold reduction of base-base conversion and the change in protein coding gene,Yichuan Xuebao(Genetic Journal),(5):403-408(卢柏松,黄培堂,1996,蛋白编码基因中四倍简并位点碱基转换与颠换的规律研究,遗传学报,(5):403-408)
LüW.L.,Zhang X.L.,Ma X.J.,and Zhang G.H.,2014,Polymorphism of TLR9 gene in sheep in different areas of Gansu Shengwujis Tongbao(Biotechnology Notification),(5):107-111(吕伟丽,张小丽,马小军,张国华,2014,甘肃不同地区绵羊TLR9基因多态性分析,生物技术通报,(5):107-111)
LüW.L.,2014,Correlation analysis of TLR4 and TLR9 gene polymorphism and respiratory diseases in Gansu province,Thesis for M.S.,Gansu Agricultural University,Supervisor:M.L.J.,pp.7-30(吕伟丽,2014,甘肃地区小尾寒羊TLR4、TLR9基因多态性与呼吸系统疾病的相关性分析,硕士学位论文,甘肃农业大学,导师:马小军,pp.7-30)
Periasamy K.,Pichler R.,Poli M.,Cristel S.,CetráB.,Medus D.,Basar M.,Ramasamy S.,Ellahi M.B.,Mohammed F.,Teneva A.,Shamsuddin M.,Podesta M.G.,and Diallo A.,2014,Candidate gene approach for parasite resistance in sheep-variation in immune pathway genes and association with fecal egg count,PLoS One,9(2):e88337
Sarafidou T.,Stamatis C.,Kalozoumi G.,Spyrou V.,Fthenakis G.C.,Billinis C.,and Mamuris Z.,2013,Toll like receptor 9(TLR9)polymorphism G520R in sheep is associated with seropositivity for small ruminant Lentivirus,PLoS One,8(5):e63901
Selvam R.,Murali N.,Thiruvenkadan A.K.,Saravanakumar R.,Ponnudurai G.,and Jawahar T.P.,2017,Single-nucleotide polymorphism-based genetic diversity analysis of the Kilakarsal and Vembur sheep breeds,Vet.World,10(5):549-555
Tan R.,Shu R.,and Cong Y.L.,2011,The development and utilization achievements and problems of genetic resources of Xinjiang sheep,Caoshi Jiachu(Plant-eating Livestock),151(2):1-3(谈锐,舒展,丛英利,2011,新疆羊遗传资源开发利用的成就与问题,草食家畜,151(2):1-3)
Tian D.,Zeng Z.,Lu H.Y.,Tian B.G.,and Cui J.J.,2007,The research progress of Toll sample receptor and its gene polymorphism and infectious disease,Shijie Huaren Xiaohua Zazhi(World Chinese Digest Magazine),15(12):1393-1399(田地,曾争,陆海英,田国保,崔建军,2007,Toll样受体及其基因多态性与感染性疾病关系的研究进展,世界华人消化杂志,15(12):1393-1399)
Yuan X.H.,Zhang L.X.,Li M.,and Zhou Z.X.,2004,The disease mechanism and the breeding of disease resistance,Tianjin Nongxueyuan Xuebao(Journal of Tianjin Agricultural College),11(2):42-47(亓小红,张丽香,李明,周锺信,2004,家畜抗病机理和抗病育种,天津农学院学报,11(2):42-47)
Zeng X.C.,2010,The research on Xinjiang sheep genetic diversity and main economic characters candidate gene,Thesis for M.S.,Shihezi University,Supervisor:Jia B.,and Zhao Z.S.,pp.16-17(曾献存,2010,新疆绵羊遗传多样性及主要经济性状候选基因研究,硕士学位论文,石河子大学,导师:贾斌,赵宗胜,pp.16-17)
Zhou H.,and Hickford J.G.,2008,Polymorphism of Toll-like receptor 9(TLR9)gene insheep,Vet.Immunol.Immunopathol.,121(1):140-143
Kotze A.,and Muller G.H.,1994,Genetic relationship in South African cattle breeds.In:Proceedings of the 5th world congress on genetics applied to livestock production,Guelph Canada,University of Guelph,Guelph,Ontario,Canada,21413-416
Lee C.Y.,Qin J.,Munyard K.A.,Subramaniam N.S.,Wetherall JD.,Stear M.J.and Groth D.M.,2012,Conserved haplotype blocks within the sheep MHC and low SNP heterozygosity in the Class IIa subregion,Anim.Genet.,43(3):429-437
Li G.L.,Mu S.M.,Li Z.Q.,and Kang X.J.,2006,TLR-specific receptors in natural immunity,Shengming De Huaxue(Chemistry of Life),26(6):495-497(李根亮,穆淑梅,李彦芹,康现江,2006,TLR-天然免疫中的特异性受体,生命的化学,26(6):495-497)
Lu B.S.,and Huang P.T.,1996,The study of the four-fold reduction of base-base conversion and the change in protein coding gene,Yichuan Xuebao(Genetic Journal),(5):403-408(卢柏松,黄培堂,1996,蛋白编码基因中四倍简并位点碱基转换与颠换的规律研究,遗传学报,(5):403-408)
LüW.L.,Zhang X.L.,Ma X.J.,and Zhang G.H.,2014,Polymorphism of TLR9 gene in sheep in different areas of Gansu Shengwujis Tongbao(Biotechnology Notification),(5):107-111(吕伟丽,张小丽,马小军,张国华,2014,甘肃不同地区绵羊TLR9基因多态性分析,生物技术通报,(5):107-111)
LüW.L.,2014,Correlation analysis of TLR4 and TLR9 gene polymorphism and respiratory diseases in Gansu province,Thesis for M.S.,Gansu Agricultural University,Supervisor:M.L.J.,pp.7-30(吕伟丽,2014,甘肃地区小尾寒羊TLR4、TLR9基因多态性与呼吸系统疾病的相关性分析,硕士学位论文,甘肃农业大学,导师:马小军,pp.7-30)
Periasamy K.,Pichler R.,Poli M.,Cristel S.,CetráB.,Medus D.,Basar M.,Ramasamy S.,Ellahi M.B.,Mohammed F.,Teneva A.,Shamsuddin M.,Podesta M.G.,and Diallo A.,2014,Candidate gene approach for parasite resistance in sheep-variation in immune pathway genes and association with fecal egg count,PLoS One,9(2):e88337
Sarafidou T.,Stamatis C.,Kalozoumi G.,Spyrou V.,Fthenakis G.C.,Billinis C.,and Mamuris Z.,2013,Toll like receptor 9(TLR9)polymorphism G520R in sheep is associated with seropositivity for small ruminant Lentivirus,PLoS One,8(5):e63901
Selvam R.,Murali N.,Thiruvenkadan A.K.,Saravanakumar R.,Ponnudurai G.,and Jawahar T.P.,2017,Single-nucleotide polymorphism-based genetic diversity analysis of the Kilakarsal and Vembur sheep breeds,Vet.World,10(5):549-555
Tan R.,Shu R.,and Cong Y.L.,2011,The development and utilization achievements and problems of genetic resources of Xinjiang sheep,Caoshi Jiachu(Plant-eating Livestock),151(2):1-3(谈锐,舒展,丛英利,2011,新疆羊遗传资源开发利用的成就与问题,草食家畜,151(2):1-3)
Tian D.,Zeng Z.,Lu H.Y.,Tian B.G.,and Cui J.J.,2007,The research progress of Toll sample receptor and its gene polymorphism and infectious disease,Shijie Huaren Xiaohua Zazhi(World Chinese Digest Magazine),15(12):1393-1399(田地,曾争,陆海英,田国保,崔建军,2007,Toll样受体及其基因多态性与感染性疾病关系的研究进展,世界华人消化杂志,15(12):1393-1399)
Yuan X.H.,Zhang L.X.,Li M.,and Zhou Z.X.,2004,The disease mechanism and the breeding of disease resistance,Tianjin Nongxueyuan Xuebao(Journal of Tianjin Agricultural College),11(2):42-47(亓小红,张丽香,李明,周锺信,2004,家畜抗病机理和抗病育种,天津农学院学报,11(2):42-47)
Zeng X.C.,2010,The research on Xinjiang sheep genetic diversity and main economic characters candidate gene,Thesis for M.S.,Shihezi University,Supervisor:Jia B.,and Zhao Z.S.,pp.16-17(曾献存,2010,新疆绵羊遗传多样性及主要经济性状候选基因研究,硕士学位论文,石河子大学,导师:贾斌,赵宗胜,pp.16-17)
Zhou H.,and Hickford J.G.,2008,Polymorphism of Toll-like receptor 9(TLR9)gene insheep,Vet.Immunol.Immunopathol.,121(1):140-143
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |