络合剂PVP-K30对聚维酮碘杀菌效果的影响及效果改进
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摘要
目的研究络合剂PVP-K30对聚维酮碘杀菌效果的影响和效果改进。方法采用两种来源的络合剂,以相同方法与碘粉络合,配制聚维酮碘A、B,并选择效果较好的络合剂配方,加入碘酸钾增效,制备聚维酮碘C。对3种聚维酮碘以金黄色葡萄球菌进行悬液定量杀菌试验,并测定3种配方的pH和稳定性。结果原液对含3%牛血清白蛋白菌悬液中的金黄色葡萄球菌作用3min,聚维酮碘A的杀灭对数值>5.00,聚维酮碘B的杀灭对数值仅为2.31;聚维酮碘C原液作用0.5min对金黄色葡萄球菌的杀灭对数值>5.00。A、B、C配方聚维酮碘的pH值分别为3.4、3.4、4.0;54℃存放14天后,有效碘下降率分别为12.10%、21.43%、7.86%;37℃存放3个月的有效碘下降率分别为10.54%、17.79%、5.29%。结论络合剂对聚维酮碘的金黄色葡萄球菌杀菌效果有影响,碘酸钾对聚维酮碘具有增效作用。
Objective To study the effect of complex agent PVP-K30 on germicidal efficacy of povidone-iodine and its improvement.Methods Two kinds of complexing agents were used to complex with iodine powder by the same method to prepare povidone-iodine solution A and B;the complexing agent formula with better effect was selected,then potassium iodate was added synergistically to prepare povidone-iodine solution C.The suspension quantitative bactericidal test was conducted using above 3 povidone-iodine solutions for sterilization of Staphylococcus aureus;the pH and stability of the 3 formulations were determined.Results Three solutions were applied to Staphylococcus aureus containing 3% bovine serum albumin suspension for 3 min,the killing logarithm of povidone iodine A was> 5.00,and the killing logarithm of povidone iodine B was only2.31.The killing logarithm of the povidone iodine C solution for 0.5 min treatment against Staphylococcus aureus was>5.00.The pH values of 3 povidone-iodine solutions A,B and C were 3.4,3.4 and 4.0 respectively.After 14 dstorage at 54 ℃,the effective iodine reduction rates were 12.10%,21.43%and 7.86%,respectively.After storage at 37℃for 3 months,the effective iodine reduction rates were 10.54%,17.79% and 5.29%,respectively.Conclusion The complexing agent has an effect on the bactericidal efficiency of povidone-iodine for Staphylococcus aureus.Potassium iodate has a synergistic effect on povidone-iodine.
Objective To study the effect of complex agent PVP-K30 on germicidal efficacy of povidone-iodine and its improvement.Methods Two kinds of complexing agents were used to complex with iodine powder by the same method to prepare povidone-iodine solution A and B;the complexing agent formula with better effect was selected,then potassium iodate was added synergistically to prepare povidone-iodine solution C.The suspension quantitative bactericidal test was conducted using above 3 povidone-iodine solutions for sterilization of Staphylococcus aureus;the pH and stability of the 3 formulations were determined.Results Three solutions were applied to Staphylococcus aureus containing 3% bovine serum albumin suspension for 3 min,the killing logarithm of povidone iodine A was> 5.00,and the killing logarithm of povidone iodine B was only2.31.The killing logarithm of the povidone iodine C solution for 0.5 min treatment against Staphylococcus aureus was>5.00.The pH values of 3 povidone-iodine solutions A,B and C were 3.4,3.4 and 4.0 respectively.After 14 dstorage at 54 ℃,the effective iodine reduction rates were 12.10%,21.43%and 7.86%,respectively.After storage at 37℃for 3 months,the effective iodine reduction rates were 10.54%,17.79% and 5.29%,respectively.Conclusion The complexing agent has an effect on the bactericidal efficiency of povidone-iodine for Staphylococcus aureus.Potassium iodate has a synergistic effect on povidone-iodine.
引文
[1]王玲,谈智,张伟,等.邻苯二甲醛消毒液对医疗器械消毒效果的评价[J].江苏预防医学,2016,27(1):105-106.
[2]张亚增,张亚尼,宋晖,等.市场抽检九种含碘消毒剂消毒性能观察[J].中国消毒学杂志,2007,24(1):46-47.
[3]张亚增,宋晖,张亚尼,等.市售四种含碘消毒剂杀菌效果观察[J].中国消毒学杂志,2013,30(11):1022-1023.
[4]魏兰芬,潘协商,倪娜,等.聚维酮碘杀灭金黄色葡萄球菌效果与浓度的关系[J].中国消毒学杂志,2016,33(10):948-950.
[5]李俐,葛洪,王立平,等.戊二醛复方中辅助成分对其性能影响的研究[J].中国消毒学杂志,2004,21(4):305-308.
[6]于溟雪,张文福.一元固体二氧化氯消毒剂配方的研制[J].中国消毒学杂志,2012,29(4):267-268,272.
[7]卫生部卫生法制与监督司.消毒技术规范[M].北京:中华人民共和国卫生部,2002:15-126.
[8]国家药典委员会.中华人民共和国药典2015年版二部[M].北京:中国医药科技出版社,2015:1497.
[9]孙守红,李新武,黄美卿,等.两种方法保存消毒剂化学稳定性的实验研究[J].中国消毒学杂志,2003,20(1):10-14.
[2]张亚增,张亚尼,宋晖,等.市场抽检九种含碘消毒剂消毒性能观察[J].中国消毒学杂志,2007,24(1):46-47.
[3]张亚增,宋晖,张亚尼,等.市售四种含碘消毒剂杀菌效果观察[J].中国消毒学杂志,2013,30(11):1022-1023.
[4]魏兰芬,潘协商,倪娜,等.聚维酮碘杀灭金黄色葡萄球菌效果与浓度的关系[J].中国消毒学杂志,2016,33(10):948-950.
[5]李俐,葛洪,王立平,等.戊二醛复方中辅助成分对其性能影响的研究[J].中国消毒学杂志,2004,21(4):305-308.
[6]于溟雪,张文福.一元固体二氧化氯消毒剂配方的研制[J].中国消毒学杂志,2012,29(4):267-268,272.
[7]卫生部卫生法制与监督司.消毒技术规范[M].北京:中华人民共和国卫生部,2002:15-126.
[8]国家药典委员会.中华人民共和国药典2015年版二部[M].北京:中国医药科技出版社,2015:1497.
[9]孙守红,李新武,黄美卿,等.两种方法保存消毒剂化学稳定性的实验研究[J].中国消毒学杂志,2003,20(1):10-14.