三七茎叶皂苷对异丙肾上腺素诱导大鼠急性心肌缺血的保护作用
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摘要
目的研究三七茎叶皂苷对异丙肾上腺素诱导的大鼠心肌缺血的保护作用,并探讨其作用机制。方法将60只健康SD大鼠随机分为对照组、模型组、地奥心血康70 mg/kg组和三七茎叶皂苷20、40、80 mg/kg组,每组各10只。地奥心血康组和三七茎叶皂苷组大鼠每日ig给药1次,连续给药14 d。对照组和模型组在前11 d ig给予同等体积蒸馏水。模型组、地奥心血康组和三七茎叶皂苷组连续3 d sc盐酸异丙肾上腺素注射液(第1天,8 mg/kg;第2天,8 mg/kg;第3天,2 mg/kg)建立急性心肌缺血模型,对照组sc同体积生理盐水。检测大鼠血清和心肌组织中乳酸脱氢酶(LDH)、肌酸激酶(CK)、天门冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)活性检测和丙二醛(MDA)含量,观察大鼠急性心肌损伤组织的病理学变化。结果与模型组比较,三七茎叶皂苷40、80mg/kg组大鼠血清中LDH、CK、AST活性显著降低,心肌组织中LDH、AST活性显著增高(P<0.05、0.01、0.001)。与模型组比较,三七茎叶皂苷80 mg/kg组大鼠血清中SOD活性显著增加,而40、80 mg/kg组MDA含量均显著降低(P<0.05);三七茎叶皂苷20、40、80 mg/kg组心肌组织中SOD活性显著增加(P<0.05、0.01、0.001),MDA含量显著降低(P<0.05)。三七茎叶皂苷80 mg/kg组可见轻度心肌纤维变性坏死灶,心肌纤维间可见少量炎性细胞浸润。结论三七茎叶皂苷通过降低受损心肌酶的释放,提高大鼠血清和心肌组织中SOD活性,降低MDA含量,从而发挥对异丙肾上腺素诱导的大鼠急性心肌缺血损伤的保护作用,其机制可能与清除氧自由基损伤有关。
Objective To study the protective effects of Panax notoginsegnoside of leaves(PnGL) on acute myocardial ischemia induced by isoprenaline in rats and its mechanism. Methods Healthy SD rats(60) were randomly divided into control group, model group, Di'ao Xinxuekang 70 mg/kg group, and PnGL 20, 40, and 80 mg/kg group, and each group had 10 rats. Rats in Di'ao Xinxuekang group and PnGL group were ig administered with drugs for 14 d, once daily. The control group and model group were ig administered with the same volume of distilled water at the first 11 d. Rats in the model group, Di'ao Xinxuekang group and Pn GL group were sc administered with Isoprenaline Hydrochloride Injection for 3 d(the first day for 8 mg/kg, the second day for 8 mg/kg, and the third day for 2 mg/kg) to establish an acute myocardial ischemia model. Rats in the control group were ig administered with normal saline of the same volume. LDH, CK, AST, SOD activity, and MDA contents in serum and myocardial tissues of rats in each group were detected, and the pathological changes of acute myocardial injury tissues of rats were observed by HE staining. ResultsCompared with the model group, the activities of LDH, CK, and AST in serum of PnGL 40 and 80 mg/kg groups were significantly decreased, while the activities of LDH and AST in myocardial tissue were significantly increased(P < 0.05, 0.01, 0.001). Compared with the model group, the SOD activity in the serum of PnGL 80 mg/kg group was significantly increased, while the MDA content in the 40 and 80 mg/kg groups was significantly decreased(P < 0.05). The activity of SOD in myocardial tissue of PnGL 20, 40, and 80 mg/kg group were significantly increased(P < 0.05, 0.01, 0.001), and the content of MDA were significantly decreased(P < 0.05). In PnGL 80 mg/kg group, mild degeneration and necrosis of myocardial fibers were observed, and a small amount of inflammatory cells infiltrated between myocardial fibers. Conclusion PnGL play a protective role on isoprenaline-induced acute myocardial ischemia injury in rats by reducing the release of damaged myocardial enzymes, increasing SOD activity in serum and myocardial tissue, and reducing MDA content. Its mechanism of anti-myocardial ischemia injury may be related to scavenging oxygen free radicals.
Objective To study the protective effects of Panax notoginsegnoside of leaves(PnGL) on acute myocardial ischemia induced by isoprenaline in rats and its mechanism. Methods Healthy SD rats(60) were randomly divided into control group, model group, Di'ao Xinxuekang 70 mg/kg group, and PnGL 20, 40, and 80 mg/kg group, and each group had 10 rats. Rats in Di'ao Xinxuekang group and PnGL group were ig administered with drugs for 14 d, once daily. The control group and model group were ig administered with the same volume of distilled water at the first 11 d. Rats in the model group, Di'ao Xinxuekang group and Pn GL group were sc administered with Isoprenaline Hydrochloride Injection for 3 d(the first day for 8 mg/kg, the second day for 8 mg/kg, and the third day for 2 mg/kg) to establish an acute myocardial ischemia model. Rats in the control group were ig administered with normal saline of the same volume. LDH, CK, AST, SOD activity, and MDA contents in serum and myocardial tissues of rats in each group were detected, and the pathological changes of acute myocardial injury tissues of rats were observed by HE staining. ResultsCompared with the model group, the activities of LDH, CK, and AST in serum of PnGL 40 and 80 mg/kg groups were significantly decreased, while the activities of LDH and AST in myocardial tissue were significantly increased(P < 0.05, 0.01, 0.001). Compared with the model group, the SOD activity in the serum of PnGL 80 mg/kg group was significantly increased, while the MDA content in the 40 and 80 mg/kg groups was significantly decreased(P < 0.05). The activity of SOD in myocardial tissue of PnGL 20, 40, and 80 mg/kg group were significantly increased(P < 0.05, 0.01, 0.001), and the content of MDA were significantly decreased(P < 0.05). In PnGL 80 mg/kg group, mild degeneration and necrosis of myocardial fibers were observed, and a small amount of inflammatory cells infiltrated between myocardial fibers. Conclusion PnGL play a protective role on isoprenaline-induced acute myocardial ischemia injury in rats by reducing the release of damaged myocardial enzymes, increasing SOD activity in serum and myocardial tissue, and reducing MDA content. Its mechanism of anti-myocardial ischemia injury may be related to scavenging oxygen free radicals.
引文
[1]周家明,崔秀明,曾鸿超,等.三七茎叶的综合开发利用[J].现代中药研究与实践, 2009, 23(3):32-34.
[2]任小宇,孙桂波,张强,等.三七茎叶皂苷对大鼠离体工作心脏血流动力学的影响[J].微循环学杂志,2011, 21(3):14-16.
[3]任小宇,孙桂波,张强,等.三七茎叶皂苷对大鼠离体心脏缺血/再灌注损伤的保护作用[J].中国药理学通报, 2012, 28(1):92-96.
[4]李娜,陈涛,付春,等.苦心滴丸对大鼠异丙肾上腺素性缺血心肌的保护作用[J].吉林中医药, 2011,31(2):169-170.
[5]路培培,刘俊田,刘娜.鹿衔草总黄酮对异丙肾上腺素诱导的大鼠急性心肌缺血的保护作用[J].中药材,2010, 33(1):73-76.
[6]刘瑜,林蓉,张慧,等.补阳还五汤对异丙肾上腺素诱导大鼠心肌缺血的保护作用[J].中药材, 2009,32(3):380-383.
[7]杨勇,容蓉,陈明强,等.异丙肾上腺素诱导小鼠急性心肌缺血模型的方法比较[J].中药药理与临床,2010, 26(5):153-154.
[8]张云,王阶,郭丽丽.异丙肾上腺素诱导心肌缺血损伤模型的研究进展[J].医学综述, 2010, 16(23):3527-3531.
[9]崔杰峰,潘柏申.急性心肌梗死血清酶标志物沿革[J].上海医学检验杂志, 2000, 15(1):13-14.
[10]郭玮,宋斌斌,苏曦,等.心肌标志物在急性心肌梗死诊断时的临界值分析[J].上海医学检验杂志,2003, 18(1):12-14.
[11]车虎森.急性心肌梗死患者3项检测指标的临床价值[J].国际检验医学杂志, 2008, 29(3):282-283.
[12]彭晖,文锟,王明建,等. c TnL联合心肌酶检测在急性心肌梗死诊断中的意义[J].国际检验医学杂志,2010, 31(11):1344-1345.
[13] Wang S B, Tian S, Yang F, et al. Cardioprotective effect of salvianolic acid A on isoproterenol-induced myocardial infarction in rats[J]. Eur J Pharmacol, 2009, 615(1-3):125-132.
[14] Karthikeyan K, Sarala Bai B R, Niranjali Devaraj S. Grape seed proanthocyanidins ameliorates isoproterenol-induced myocardial injury in rats by stabilizing mitochondrial and lysosomal enzymes:an in vivo study[J]. Life Sci, 2007,81(23-24):1615-1621.
[15] Zhou R, Xu Q, Zheng P, et al. Cardioprotective effect of fluvastatin on isoproterenol-induced myocardial infarction in rat[J]. Eur J Pharmacol, 2008, 586(1-3):244-250.
[2]任小宇,孙桂波,张强,等.三七茎叶皂苷对大鼠离体工作心脏血流动力学的影响[J].微循环学杂志,2011, 21(3):14-16.
[3]任小宇,孙桂波,张强,等.三七茎叶皂苷对大鼠离体心脏缺血/再灌注损伤的保护作用[J].中国药理学通报, 2012, 28(1):92-96.
[4]李娜,陈涛,付春,等.苦心滴丸对大鼠异丙肾上腺素性缺血心肌的保护作用[J].吉林中医药, 2011,31(2):169-170.
[5]路培培,刘俊田,刘娜.鹿衔草总黄酮对异丙肾上腺素诱导的大鼠急性心肌缺血的保护作用[J].中药材,2010, 33(1):73-76.
[6]刘瑜,林蓉,张慧,等.补阳还五汤对异丙肾上腺素诱导大鼠心肌缺血的保护作用[J].中药材, 2009,32(3):380-383.
[7]杨勇,容蓉,陈明强,等.异丙肾上腺素诱导小鼠急性心肌缺血模型的方法比较[J].中药药理与临床,2010, 26(5):153-154.
[8]张云,王阶,郭丽丽.异丙肾上腺素诱导心肌缺血损伤模型的研究进展[J].医学综述, 2010, 16(23):3527-3531.
[9]崔杰峰,潘柏申.急性心肌梗死血清酶标志物沿革[J].上海医学检验杂志, 2000, 15(1):13-14.
[10]郭玮,宋斌斌,苏曦,等.心肌标志物在急性心肌梗死诊断时的临界值分析[J].上海医学检验杂志,2003, 18(1):12-14.
[11]车虎森.急性心肌梗死患者3项检测指标的临床价值[J].国际检验医学杂志, 2008, 29(3):282-283.
[12]彭晖,文锟,王明建,等. c TnL联合心肌酶检测在急性心肌梗死诊断中的意义[J].国际检验医学杂志,2010, 31(11):1344-1345.
[13] Wang S B, Tian S, Yang F, et al. Cardioprotective effect of salvianolic acid A on isoproterenol-induced myocardial infarction in rats[J]. Eur J Pharmacol, 2009, 615(1-3):125-132.
[14] Karthikeyan K, Sarala Bai B R, Niranjali Devaraj S. Grape seed proanthocyanidins ameliorates isoproterenol-induced myocardial injury in rats by stabilizing mitochondrial and lysosomal enzymes:an in vivo study[J]. Life Sci, 2007,81(23-24):1615-1621.
[15] Zhou R, Xu Q, Zheng P, et al. Cardioprotective effect of fluvastatin on isoproterenol-induced myocardial infarction in rat[J]. Eur J Pharmacol, 2008, 586(1-3):244-250.