DNA碱基激光拉曼光谱检测方法的研究
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摘要
以激光拉曼光谱仪采集腺嘌呤(A)、鸟嘌呤(G)、胞嘧啶(C)、胸腺嘧啶(T)的拉曼光谱图,分析了各碱基谱峰归属,并依据归属结果检测混合碱基组成。结果显示:4种碱基的特征峰集中在175～1 800cm~(-1)。相同积分时间和激光功率条件下,该波段内嘌呤碱基的激光拉曼光谱峰高于嘧啶碱基的谱峰。腺嘌呤、鸟嘌呤、胞嘧啶、胸腺嘧啶分别在723、648、791、1 368 cm~(-1)处的谱峰最强,利用这些特征峰能在2min内鉴别出混合碱基中的各碱基。
A method of laser Raman spectrometry was developed for the detection of DNA bases. The Raman spectra of adenine( A),guanine( G),cytosine( C) and thymine( T) were collected with laser Raman spectrometer in this work. The characteristic peaks in the spectra for each DNA base were analyzed,which were used to determine the components of DNA base mixtures. Results demonstrated that the characteristic peaks mainly ranged from 175 cm~(-1) to 1 800 cm~(-1). The peaks of A and G were higher than those of C and T when the integration time and laser power were certain. The strongest Raman signals for A,G,C,and T are at 723,648,791 and 1 368 cm~(-1),respectively.Each DNA base could be determined by using the characteristic peaks from the mixed DNA bases within 2 min.
A method of laser Raman spectrometry was developed for the detection of DNA bases. The Raman spectra of adenine( A),guanine( G),cytosine( C) and thymine( T) were collected with laser Raman spectrometer in this work. The characteristic peaks in the spectra for each DNA base were analyzed,which were used to determine the components of DNA base mixtures. Results demonstrated that the characteristic peaks mainly ranged from 175 cm~(-1) to 1 800 cm~(-1). The peaks of A and G were higher than those of C and T when the integration time and laser power were certain. The strongest Raman signals for A,G,C,and T are at 723,648,791 and 1 368 cm~(-1),respectively.Each DNA base could be determined by using the characteristic peaks from the mixed DNA bases within 2 min.
引文
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[3] Jiang L H,Shen J,Yu Z H,Shao Y N,Chen X W,Chen X D. Opt. Instrum.(蒋林华,沈俊,余治昊,邵咏妮,陈小婉,陈晓东.光学仪器),2018,40(2):31-37.
[4] Chen Z Y,Lu Y L,Liang Y,Zeng C,Zhang Z M,Li G K. J. Instrum. Anal.(陈正毅,卢雅琳,梁豫,曾晨,张卓旻,李攻科.分析测试学报),2017,36(5):650-654.
[5] Felipe M F,Alan L V,JoséA G N,George L D,Bradley T J. Microchem. J.,2017,(133):76-80.
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[8] Chen X M,Lei H Y,Hu Y L,Li G K. J. Instrum. Anal.(陈小曼,雷皓宇,胡玉玲,李攻科.分析测试学报),2016,35(10):1343-1346.
[9] Rico S,Jürgen K,Heinar S. Vib. Spectrosc.,2014,(70):12-17.
[10] Peng Y,Zhang Z M,Lu H M,Liang Y Z,Liu C,Chen Q Z,Liu G K. J. Instrum. Anal.(彭颖,张志敏,卢红梅,梁逸曾,刘察,陈启振,刘国坤.分析测试学报),2017,36(5):627-632.
[11] Wan Q E,Liu H P,Zhang H M,Liu S H. Spectrosc. Spectral Anal.(万秋娥,刘汉平,张鹤鸣,刘颂豪.光谱学与光谱分析),2012,32(4):989-992.
[12] Huang X L,Zhan Y L,Li J,Zhang C B,Wang X Q,Tang L N. Sci. Technol. Food Ind.(黄秀丽,詹云丽,李菁,张长斌,王晓琴,唐丽娜.食品工业科技),2016,37(20):59-62,67.
[13] Meng Q G,Liu C L,He X L,Ye Y G,Zhu Y H,Xia N. Geol. Bull. Chin.(孟庆国,刘昌岭,贺行良,业渝光,祝有海,夏宁.地质通报),2011,30(12):1863-1867.
[14] Ding J Y,Ni P,Rao B,Zhou J,Zhu X T. Geol. Rev.(丁俊英,倪培,饶冰,周进,朱筱婷.地质论评),2004,(2):203-209.
[15] Nicola F,Emily D M,Andrew H K,Christian H,Roger E S. Carbon,2016,(108):440-449.
[16] Mao D Z,Weng X X,Yang Y J. Chin. J. Pharm.(毛丹卓,翁欣欣,杨永健.中国医药工业杂志),2012,43(9):776-779.
[17] Dolenko T A,Burikov S A,Laptinskiy K A,Sarmanova O E. Laser Phys.,2016,26(2):025206.
[18] Fu Y,Zhang W H,Gong L,Wen J X,Liang Z,Gong Y T,Xie F Y,Chen J. J. Instrum. Anal.(浮钰,张卫红,龚力,温锦绣,梁正,巩玉婷,谢方艳,陈建.分析测试学报),2015,34(6):652-657.