3D打印钛合金牙种植体的细胞毒性的研究
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摘要
目的:利用MTT比色实验,检测以3D打印技术铸造出的钛合金牙种植体的细胞毒性,评测新型铸造工艺的可行性,为下一步的临床应用提供理论依据。方法:我们以专业软件设计建立计算机模型,选取Ti-6Al-4V、Ti-6Al-7Nb两种不同的钛合金作为铸造原材料,以3D打印技术铸造出同样规格的三种实验标准片。实验主要分为3组,即Ti-6Al-4V组、Ti-6Al-7Nb组、空白对照组。通过MTT比色实验方法,我们分别检测了这三种标准件对于成骨细胞MG63增殖率的影响,来完成对于3D打印技术的评价。结果:在倒置相差显微镜下我们观察到:三组细胞的生长状态均良好,实验组与空白对照组相比,细胞形态无显著区别。MTT比色结果显示,两组钛片的细胞毒性分级均为0级,同时,Ti-6Al-7Nb钛合金片增殖率略高于Ti-6Al-4V钛合金片浸提液组。结论:通过3D打印技术铸造出的的钛合金种植体,其细胞毒性水平满足口腔种植材料临床应用的要求,且Ti-6Al-7Nb的细胞毒性水平略低于Ti-6Al-4V。
Objective: MTT colorimetric assay was used to detect the cytotoxicity of titanium alloy dental implant cast by 3D printing technology, and to evaluate the feasibility of the new casting process, so as to provide theoretical basis for further clinical application. Methods: We used professional software design to build the computer model, selected two different titanium alloys Ti-6 Al-4V and Ti-6Al-7Nb as the casting raw materials, and cast three kinds of experimental standard plates of the same specification by 3D printing technology. The experiment was mainly divided into three groups, namely Ti-6Al-4V group, Ti-6Al-7Nb group and blank control group. By MTT colorimetric assay, we tested the effects of these three standard components on the proliferation rate of osteoblast MG63 to complete the evaluation of 3D printing technology. Results: Under the inverted phase contrast microscope, we observed that the growth status of cells in the three groups were all good, and there was no significant difference in cell morphology between the experimental group and the blank control group. MTT colorimetric results showed that the cytotoxicity level of the two groups of titanium sheets was 0. Meanwhile, the proliferation rate of Ti-6Al-7Nb titanium alloy sheets was slightly higher than that of Ti-6Al-4V titanium alloy sheet extraction solution group. Conclusion: The cytotoxicity level of titanium alloy implants cast by 3D printing technology meets the requirements of clinical application of oral implant materials, and the cytotoxicity level of Ti-6Al-7Nb is slightly lower than that of Ti-6Al-4V
Objective: MTT colorimetric assay was used to detect the cytotoxicity of titanium alloy dental implant cast by 3D printing technology, and to evaluate the feasibility of the new casting process, so as to provide theoretical basis for further clinical application. Methods: We used professional software design to build the computer model, selected two different titanium alloys Ti-6 Al-4V and Ti-6Al-7Nb as the casting raw materials, and cast three kinds of experimental standard plates of the same specification by 3D printing technology. The experiment was mainly divided into three groups, namely Ti-6Al-4V group, Ti-6Al-7Nb group and blank control group. By MTT colorimetric assay, we tested the effects of these three standard components on the proliferation rate of osteoblast MG63 to complete the evaluation of 3D printing technology. Results: Under the inverted phase contrast microscope, we observed that the growth status of cells in the three groups were all good, and there was no significant difference in cell morphology between the experimental group and the blank control group. MTT colorimetric results showed that the cytotoxicity level of the two groups of titanium sheets was 0. Meanwhile, the proliferation rate of Ti-6Al-7Nb titanium alloy sheets was slightly higher than that of Ti-6Al-4V titanium alloy sheet extraction solution group. Conclusion: The cytotoxicity level of titanium alloy implants cast by 3D printing technology meets the requirements of clinical application of oral implant materials, and the cytotoxicity level of Ti-6Al-7Nb is slightly lower than that of Ti-6Al-4V
引文
1周仲炎,庄素国,Ti-6Al-4V合金激光原位合成自润滑复合涂层高温摩擦学性能[J].材料工程,2019-03 vol(47):101-108
2丁子彧,丁泽良.医用植入材料Ti6Al4V的腐蚀与防腐研究进展[J].包装学报,2018,vol10(3):84-90
3马凯,赵宝红,邓春富.医用钛及钛合金牙种植体生物相容性及其相关抗菌性能研究进展[J].中国实用口腔科杂志,2016,7 vol9(7):441-445
4王聪.刘永杰,模拟体液环境下植入用Ti6Al7Nb合金的高周疲劳行为[J].实验力学,2016,vol36(6):723-729
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8 Gottlow J,Dard M,Kjellson F,et al.Evaluation of a new titanium-zirconium dental implant:A biomechanical and histological comparative study in the mini pig[J].Clinical implant dentistry and related research,2012,14(4):538-545
9张一迪.应用于牙科种植体的新型钛合金生物安全性检测,2016,5,吉林大学硕士学位论文
10 Wang X,Liu H,Ren X,et al.Effects of fluorideion-implanted titanium surface on the cytocompatibility in vitro and osseointegatation in vivo for dental implant applications[J].Colloids and Surfaces B:Biointerfaces,2015,136:752-760
11 Niinomi M.Biologically and mechanically biocompatible titanium alloys[J].Materials transactions.2008,49(10):2170-8
12王革.钛及钛合金表面钝化膜的特点及其形成机理.中国口腔种植学杂志,2002,3,5(1):37~39
13 QiuWeiliu(邱蔚六).China Reconstructive Surgery(中国修复重建外科杂志)[J],2006,20(4):339
14 LiuFei.American Society of Biomechanics Newsletter[J],2009,22(1):11
15 Giannatsis J,Dedoussis V.Int J AdvManufTechnol[J],2009,40:116
16 HoratiuRotaru,Horatiu Stan,Ioan Stefan Florian et al.Journal of Oral and Maxillofacial Surgery[J],2012,70(2):169
2丁子彧,丁泽良.医用植入材料Ti6Al4V的腐蚀与防腐研究进展[J].包装学报,2018,vol10(3):84-90
3马凯,赵宝红,邓春富.医用钛及钛合金牙种植体生物相容性及其相关抗菌性能研究进展[J].中国实用口腔科杂志,2016,7 vol9(7):441-445
4王聪.刘永杰,模拟体液环境下植入用Ti6Al7Nb合金的高周疲劳行为[J].实验力学,2016,vol36(6):723-729
5 Wang Y,Yu H,Chen C,et al.Review of the biocompatibility of micro-arc oxidation coated titanium alloys[J].Materials&Design,2015,85:640-652
6 Miura K,Yamada N,Hanada S,et al.The bone tissue compatibility of a new Ti-Nb-Sn alloy with a low Young's modulus[J].Acta Biomaterialia,2011,7(5):2320-2326
7 Fukuda A,Takemoto M,Saito T,et al.Bone bonding bioactivity of Ti metal and Ti-Zr-Nb-Ta alloys with Ca ions incorporated on their surfaces by simple chemical and heat treatments[J].Actabiomaterialia,2011,7(3):1379-1386
8 Gottlow J,Dard M,Kjellson F,et al.Evaluation of a new titanium-zirconium dental implant:A biomechanical and histological comparative study in the mini pig[J].Clinical implant dentistry and related research,2012,14(4):538-545
9张一迪.应用于牙科种植体的新型钛合金生物安全性检测,2016,5,吉林大学硕士学位论文
10 Wang X,Liu H,Ren X,et al.Effects of fluorideion-implanted titanium surface on the cytocompatibility in vitro and osseointegatation in vivo for dental implant applications[J].Colloids and Surfaces B:Biointerfaces,2015,136:752-760
11 Niinomi M.Biologically and mechanically biocompatible titanium alloys[J].Materials transactions.2008,49(10):2170-8
12王革.钛及钛合金表面钝化膜的特点及其形成机理.中国口腔种植学杂志,2002,3,5(1):37~39
13 QiuWeiliu(邱蔚六).China Reconstructive Surgery(中国修复重建外科杂志)[J],2006,20(4):339
14 LiuFei.American Society of Biomechanics Newsletter[J],2009,22(1):11
15 Giannatsis J,Dedoussis V.Int J AdvManufTechnol[J],2009,40:116
16 HoratiuRotaru,Horatiu Stan,Ioan Stefan Florian et al.Journal of Oral and Maxillofacial Surgery[J],2012,70(2):169