基于“入腹知性”理论的黄芩干预大鼠肠道菌群多样性的探讨
|
摘要
目的:探讨黄芩对生理状态下大鼠肠道菌群多样性的变化,分析并判定黄芩药性的归属。方法:利用16S rRNA高通量基因测序技术对黄芩给药(10 g·kg~(-1))干预后的大鼠盲肠内溶物进行检测,将肠道菌群分类单元(OTUs)数量,丰富度与多样性指数及差异菌门与菌属进行综合性分析,并利用网络可视化手段找寻差异菌门与菌属之间的关联性。结果:基于Illumina Miseq平台,与空白组相比,黄芩给药组大鼠肠道菌群的OTUs数量减少,丰富度与多样性指数降低;拟杆菌门(Bacteroidetes)和螺旋体菌门(Spirochaetes)显著性上调(P <0. 01),厚壁菌门(Firmicutes),放线菌门(Actinobacteria),变形菌门(Proteobacteria)及软壁菌门(Tenericutes)显著性下调(P <0. 01),24个差异菌属中有17个显著性下调(P <0. 05,P <0. 01),7种显著性上调(P <0. 01)。联合网络可视化分析确定影响程度最大的菌门为拟杆菌门与厚壁菌门,其中厚壁菌门与其余菌门多呈现出负相关的关系,与螺旋体菌门存在最强负相关性;拟杆菌门与变形菌门、厚壁菌门呈正相关的关系,与放线菌门呈现负相关的关系,与其余菌门无相关性相关性;差异菌属多富集于瘤胃球菌属(Ruminococcus),帕拉普氏菌属(Paraprevotella),普雷沃菌属(Prevotella)及颤螺旋菌属(Oscillospira)。结论:黄芩可降低肠道菌群多样性,抑制机体的菌群代谢功能,因此从菌群多样性角度推断黄芩的药性为寒凉。
Objective: To explore the effect of Scutellariae Radix on the diversity of intestinal flora in rats under physiological conditions,in order to determine the property of Scutellariae Radix property. Method: 16 S rRNA high-throughput gene sequencing technique was used to detect the cecum solutes of rats treated with Scutellariae Radix( 10 g·kg~(-1)). The number,richness and diversity index of the intestinal flora taxon( OTUs)and the differential phylum and genus were comprehensively analyzed. The network visualization was used to find the correlation between differential phylum and genus. Result: Based on the Illumina Miseq platform,compared with the blank group,the number of OTUs and the index of richness and diversity of the intestinal flora of the rats treated with Scutellariae Radix decreased. Bacteroidetes and Spirochaetes were significantly up-regulated( P <0. 01),while Firmicutes,Actinobacteria,Proteobacteria and Tenericutes were significantly down-regulated( P <0. 01). Among the 24 differential bacteria genera,17 were significantly down-regulated( P < 0. 05,P < 0. 01),while 7 species were significantly up-regulated( P < 0. 01). On the basis of the network visualization analysis,the most influential phylua were Bacteroides and Firmicutes,in which Firmicutes showed a negative correlation with the rest of phylum,with the strongest negative correlation with spirochetes. There was a positive correlation between Bacteroides,Proteobacteria and Firmicutes,but a negative correlation with Actinobacteria,and no correlation with other phylum. Most of the differential bacteria were enriched in Ruminococcus,Paraprevotella,Prevotella and Oscillospira. Conclusion: Scutellariae Radix can reduce the diversity of intestinal flora and inhibit the metabolism of the body,so its property is cold.
Objective: To explore the effect of Scutellariae Radix on the diversity of intestinal flora in rats under physiological conditions,in order to determine the property of Scutellariae Radix property. Method: 16 S rRNA high-throughput gene sequencing technique was used to detect the cecum solutes of rats treated with Scutellariae Radix( 10 g·kg~(-1)). The number,richness and diversity index of the intestinal flora taxon( OTUs)and the differential phylum and genus were comprehensively analyzed. The network visualization was used to find the correlation between differential phylum and genus. Result: Based on the Illumina Miseq platform,compared with the blank group,the number of OTUs and the index of richness and diversity of the intestinal flora of the rats treated with Scutellariae Radix decreased. Bacteroidetes and Spirochaetes were significantly up-regulated( P <0. 01),while Firmicutes,Actinobacteria,Proteobacteria and Tenericutes were significantly down-regulated( P <0. 01). Among the 24 differential bacteria genera,17 were significantly down-regulated( P < 0. 05,P < 0. 01),while 7 species were significantly up-regulated( P < 0. 01). On the basis of the network visualization analysis,the most influential phylua were Bacteroides and Firmicutes,in which Firmicutes showed a negative correlation with the rest of phylum,with the strongest negative correlation with spirochetes. There was a positive correlation between Bacteroides,Proteobacteria and Firmicutes,but a negative correlation with Actinobacteria,and no correlation with other phylum. Most of the differential bacteria were enriched in Ruminococcus,Paraprevotella,Prevotella and Oscillospira. Conclusion: Scutellariae Radix can reduce the diversity of intestinal flora and inhibit the metabolism of the body,so its property is cold.
引文
[1]孙鑫,钱会南.《神农本草经》与统编中药学教材中药理论体系框架对比研究[J].环球中医药,2017,12(10):1477-1480.
[2]姜开运,梁茂新.基于象思维的中药五味理论解析[J].中医杂志,2017,58(16):1351-1354.
[3]王厚伟,秦林,田景振,等.基于中药四性的中药新药研究策略[J].世界科学技术—中医药现代化,2015,17(11):2197-2201.
[4]苑艺蕾,汪娜,韩贞爱,等.黄芩“逐水”功效的文献学考证[J].中医药信息,2014,33(6):41-43.
[5]邹国发,熊成成,陈艳芬,等.基于中药药性理论的清热药现代研究进展[J].中国实验方剂学杂志,2017,23(6):229-234.
[6]张亚男,陈平平,王洪玉,等.黄芩拆分组分寒热药性归属[J].辽宁中医药大学学报,2017,19(12):32-36.
[7]高鑫,陈平平,王喆,等.基于大鼠机体能量代谢生物标志物变化的黄芩药性研究[J].中华中医药杂志,2018,33(5):2031-2036.
[8]郭慧,崔扬,王秋红,等.代谢组学技术在中药药性理论研究中心的应用概述[J].中草药,2016,47(3):363-368.
[9]戴逸飞,霍海如,王朋倩,等.基于系统药理模式挖掘中药寒热药性的关键靶标和疾病网络[J].中华中医药杂志,2018,33(2):521-526.
[10]张亚男,陈平平,王洪玉,等.黄芩拆分组分对正常大鼠物质代谢、能量代谢、内分泌系统及植物神经系统的影响[J].天津中医药大学学报,2018,37(2):137-140.
[11]张宁,李自辉,赵洪伟,等.寒凝血瘀证大鼠的肠道菌群变化与粪便代谢特征分析[J].中国实验方剂学杂志,2018,24(2):79-85.
[12] McGovern E,Kenny D A,McCabe M S,et al. 16S rRNA sequencing reveals relationship between potent cellulolytic genera and feed efficiency in the rumen of bulls[J]. Front Microbiol,2018,9(8):1842-1856.
[13]陈秀琴,黄小洁,石达友,等.中药与肠道菌群相互作用的研究进展[J].中草药,2014,45(7):103-1036.
[14]祖先鹏,林璋,谢海胜,等.中药有效成分与肠道菌群相互作用的研究进展[J].中国中药杂志,2016,41(10):1766-1772.
[15]安婉丽,李雪丽,孔冉,等.中医药治疗肠道菌群失调症的方剂用药规律分析[J].中国实验方剂学杂志,2018,24(12):210-215.
[16]赵立平,贾娜.肠道菌群与肥胖症的关系研究进展[J].微生物与感染,2013,8(2):67-71.
[17]朱青,王晓歌,王奇,等.消脂汤对非酒精性脂肪性肝炎小鼠肠道主要菌群的影响[J].中国实验方剂学杂志,2017,23(14):164-170.
[18] Ley R E. Obesity and human microbiome[J]. Curr Opin Gastroenterol,2010,26(1):5-11.
[19]郎敏,郑克鸿,曾松华,等.肥胖2型糖尿病患者胃旁路手术后肠道菌群的变化[J].南方医科大学学报,2017,37(10):1337-1344.
[2]姜开运,梁茂新.基于象思维的中药五味理论解析[J].中医杂志,2017,58(16):1351-1354.
[3]王厚伟,秦林,田景振,等.基于中药四性的中药新药研究策略[J].世界科学技术—中医药现代化,2015,17(11):2197-2201.
[4]苑艺蕾,汪娜,韩贞爱,等.黄芩“逐水”功效的文献学考证[J].中医药信息,2014,33(6):41-43.
[5]邹国发,熊成成,陈艳芬,等.基于中药药性理论的清热药现代研究进展[J].中国实验方剂学杂志,2017,23(6):229-234.
[6]张亚男,陈平平,王洪玉,等.黄芩拆分组分寒热药性归属[J].辽宁中医药大学学报,2017,19(12):32-36.
[7]高鑫,陈平平,王喆,等.基于大鼠机体能量代谢生物标志物变化的黄芩药性研究[J].中华中医药杂志,2018,33(5):2031-2036.
[8]郭慧,崔扬,王秋红,等.代谢组学技术在中药药性理论研究中心的应用概述[J].中草药,2016,47(3):363-368.
[9]戴逸飞,霍海如,王朋倩,等.基于系统药理模式挖掘中药寒热药性的关键靶标和疾病网络[J].中华中医药杂志,2018,33(2):521-526.
[10]张亚男,陈平平,王洪玉,等.黄芩拆分组分对正常大鼠物质代谢、能量代谢、内分泌系统及植物神经系统的影响[J].天津中医药大学学报,2018,37(2):137-140.
[11]张宁,李自辉,赵洪伟,等.寒凝血瘀证大鼠的肠道菌群变化与粪便代谢特征分析[J].中国实验方剂学杂志,2018,24(2):79-85.
[12] McGovern E,Kenny D A,McCabe M S,et al. 16S rRNA sequencing reveals relationship between potent cellulolytic genera and feed efficiency in the rumen of bulls[J]. Front Microbiol,2018,9(8):1842-1856.
[13]陈秀琴,黄小洁,石达友,等.中药与肠道菌群相互作用的研究进展[J].中草药,2014,45(7):103-1036.
[14]祖先鹏,林璋,谢海胜,等.中药有效成分与肠道菌群相互作用的研究进展[J].中国中药杂志,2016,41(10):1766-1772.
[15]安婉丽,李雪丽,孔冉,等.中医药治疗肠道菌群失调症的方剂用药规律分析[J].中国实验方剂学杂志,2018,24(12):210-215.
[16]赵立平,贾娜.肠道菌群与肥胖症的关系研究进展[J].微生物与感染,2013,8(2):67-71.
[17]朱青,王晓歌,王奇,等.消脂汤对非酒精性脂肪性肝炎小鼠肠道主要菌群的影响[J].中国实验方剂学杂志,2017,23(14):164-170.
[18] Ley R E. Obesity and human microbiome[J]. Curr Opin Gastroenterol,2010,26(1):5-11.
[19]郎敏,郑克鸿,曾松华,等.肥胖2型糖尿病患者胃旁路手术后肠道菌群的变化[J].南方医科大学学报,2017,37(10):1337-1344.