利用荧光区域积分法解析鄱阳湖DOM组成及来源
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摘要
文章采用三维荧光光谱技术结合荧光区域积分(FRI)研究了鄱阳湖典型消落带水体、土壤、植物中的溶解性有机质(DOM)光谱特性,分析了DOM组成、腐质化程度、污染来源与其他水质指标之间的相关性。通过荧光区域积分法将荧光光谱图划分为5大类,进行了区域积分计算及分析。通过分析三维荧光光谱特征峰,探讨了鄱阳湖消落带的水体、土壤、植物中DOM的区别与联系。结果表明:鄱阳湖水体的三维荧光峰主要有4类,湖水的DOM主要为陆源输入为主,受人类活动影响较小。土壤的三维荧光峰主要有6类,A、C、B、T、D、E,且A、C峰强度较水体中较强,类蛋白峰相比水体较弱;土壤中DOM腐质化程度高,且分子量较大;植物中含有荧光峰种类更多,且有植物蛋白,植物中的DOM的含量较水体和土壤高。龙口南荻和龙口芦苇的r_(A/E)、r_(C/E)<1,类富里酸物质含量小于类色氨酸物质。其余的植物样品中r_(A/E)、r_(C/E)>1,DOM中类富里酸物质含量大于类色氨酸物质。
The spectral characteristics of dissolved organic matter(DOM) in water, soil and plants in the typical hydro-fluctuation zones of Poyang Lake were studied using a three-dimensional fluorescence excitation-emission matrix(3-DEEM)combined with fluorescence regional integration(FRI). The composition and source of dissolved organic matter(DOM), degree of humification, and the correlation between water quality indicators and other factors were analyzed. The fluorescence spectrum was divided into five categories using the fluorescence regional integration method, and the regional integral calculation and analysis were performed. The differences and associations of DOM in water, soil and plants in the typical hydro-fluctuation zones of Poyang Lake were discussed by analyzing the characteristic peaks of 3-DEEM. The results showed that there are four main types of three-dimensional fluorescence peaks in the water body of Poyang Lake. The DOM in the lake water was mainly input from the land source and was less affected by human activities. There were six types of three-dimensional fluorescence peaks in the soil, ACBTDE, and the intensity of A and C peaks was stronger than that in water bodies. The protein-like peaks were weaker than in water bodies; DOM in soil was highly humified and had a large molecular weight. In plants there were more types of fluorescent peaks and plant proteins, and the contents of DOM was higher than that of water and soil.The r_(A/E) and r_(C/E) of Triarrhena lutarioriparia L and reed in Longkou were less than 1, and the content of fulvic acid was less than that of tryptophan. But in another plant samples, the r_(A/E) and r_(C/E)>1, and the content of fulvic acid in DOM was greater than that of tryptophan in DOM.
The spectral characteristics of dissolved organic matter(DOM) in water, soil and plants in the typical hydro-fluctuation zones of Poyang Lake were studied using a three-dimensional fluorescence excitation-emission matrix(3-DEEM)combined with fluorescence regional integration(FRI). The composition and source of dissolved organic matter(DOM), degree of humification, and the correlation between water quality indicators and other factors were analyzed. The fluorescence spectrum was divided into five categories using the fluorescence regional integration method, and the regional integral calculation and analysis were performed. The differences and associations of DOM in water, soil and plants in the typical hydro-fluctuation zones of Poyang Lake were discussed by analyzing the characteristic peaks of 3-DEEM. The results showed that there are four main types of three-dimensional fluorescence peaks in the water body of Poyang Lake. The DOM in the lake water was mainly input from the land source and was less affected by human activities. There were six types of three-dimensional fluorescence peaks in the soil, ACBTDE, and the intensity of A and C peaks was stronger than that in water bodies. The protein-like peaks were weaker than in water bodies; DOM in soil was highly humified and had a large molecular weight. In plants there were more types of fluorescent peaks and plant proteins, and the contents of DOM was higher than that of water and soil.The r_(A/E) and r_(C/E) of Triarrhena lutarioriparia L and reed in Longkou were less than 1, and the content of fulvic acid was less than that of tryptophan. But in another plant samples, the r_(A/E) and r_(C/E)>1, and the content of fulvic acid in DOM was greater than that of tryptophan in DOM.
引文
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[12]Ylla I,Anna M Romaní,Sergi Sabater.Labile and recalcitrant organic matter utilization by river biofilm under increasing water temperature[J].Microbial Ecology,2012,64(3):593-604.
[13]Yu G H,Wu M J,Luo Y H,et al.Fluorescence excitationemission spectroscopy with regional integration analysis for assessment of compost maturity[J].Waste Management,2011,31(8):1729-1736.
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[15]Hudson N,Baker A,Ward D,et al.Can fluorescence spectrometry be used as a surrogate for the Biochemical Oxygen Demand(BOD)test in water quality assessment-an example from South West England[J].Science of the Total Environment,2008,391(1):149-158.
[16]高洁,江韬,李璐璐,等.三峡库区消落带土壤中溶解性有机质(DOM)吸收及荧光光谱特征[J].环境科学,2015,36(1):151-162.Gao Jie,Jiang Tao,Li Lulu,et al.Ultraviolet-visible(UV-Vis)and fluorescence spectral characteristics of dissolved organic matter(DOM)in soils of water-level fluctuation zones of the Three Gorges Reservoir Region[J].Environmental Science,2015,36(1):151-162.
[17]Ritchelita P Galapate,Aloysius U Baes,Kazuaki Ito,et al.Detection of domestic wastes in Kurose river using synchronous fluorescence spectroscopy[J].Water Research,1998,32(7):2232-2239.
[18]李帅东,姜泉良,黎烨,等.环滇池土壤溶解性有机质(DOM)的光谱特征及来源分析[J].光谱学与光谱分析,2017,37(5):1448-1454.Li Shuaidong,Jiang Quanliang,Li Ye,et al.Spectroscopic characteristics and sources of dissolved organic matter from soils around Dianchi Lake,Kunming[J].Spectroscopy and Spectral Analysis,2017,37(5):1448-1454.
[19]Helms J R,Stubbins A,Ritchie J D,et al.Absorption spectral slopes and slope ratios as indicators of molecular weight,source,and photobleaching of chromophoric dissolved organic matter[J].Limnology&Oceanography,2008,53(3):955-969.
[20]Xiao Y,Sara-Aho T,Hartikainen H,et al.Contribution of ferric iron to light absorption by chromophoric dissolved organic matter[J].Limnology and Oceanography,2013,58(2):653-662.
[2]隋志男,郅二铨,姚杰,等.三维荧光光谱区域积分法解析辽河七星湿地水体DOM组成及来源[J].环境工程技术学报,2015,5(2):114-120.Sui Zhinan,Zhi Erquan,Yao Jie,et al.Characterization of DOM composition and origin using three-dimensional fluorescence spectroscopy coupled with regionintegration method in Qixing wetland[J].Journal of Environmental Engineering Technology,2015,5(2):114-120.
[3]李璐璐,江韬,卢松,等.利用紫外-可见吸收光谱估算三峡库区消落带水体、土壤和沉积物溶解性有机质(DOM)浓度[J].环境科学,2014,35(9):3408-3416.Li Lulu,Jiang Tao,Lu Song,et al.Using ultraviolet-visible(UV-Vis)absorption spectrum to estimate the dissolved organic matter(DOM)concentration in water,soils and sediments of typical water-level fluctuation zones of the Three Gorges Reservoir Areas[J].Environmental Science,2014,35(9):3408-3416.
[4]宋晓娜,于涛,张远,等.利用三维荧光技术分析太湖水体溶解性有机质的分布特征及来源[J].环境科学学报,2010,30(11):2321-2331.Song Xiaona,Yu Tao,Zhang Yuan,et al.Distribution characterization and source analysis of dissolved organic matter in Taihu Lake using a three dimensional fluorescence excitation-emission matrix[J].Acta Scientiae Circumstantiae,2010,30(11):2321-2331.
[5]弓晓峰,陈春丽,周文斌,等.鄱阳湖底泥中重金属污染现状评价[J].环境科学,2006,27(4):732-736.Gong Xiaofeng,Chen Chunli,Zhou Wenbin,et al.Assessment on heavy metal pollution in the sediment of Poyang Lake[J].Environmental Science,2006,27(4):732-736.
[6]葛刚,徐燕花,赵磊,等.鄱阳湖典型湿地土壤有机质及氮素空间分布特征[J].长江流域资源与环境,2010,19(6):619-622.Ge Gang,Xu Yanhua,Zhao Lei,et al.Spatial distribution characteristics of soil organic matter and nitrogen in the Poyang Lake wetland[J].Resource and Environment in the Yangtze Basin,2010,19(6):619-622.
[7]李帅东,张明礼,杨浩,等.昆明松华坝库区表层土壤溶解性有机质(DOM)的光谱特性[J].光谱学与光谱分析,2017,37(4):1183-1188.Li Shuaidong,Zhang Mingli,Yang Hao,et al.Spectroscopic characteristics of dissolved organic matter from top soils on Songhuaba Reservoir in Kunming[J].Spectroscopy and Spectral Analysis,2017,37(4):1183-1188.
[8]谢理,杨浩,渠晓霞,等.滇池典型陆生和水生植物溶解性有机质组分的光谱分析[J].环境科学研究,2013,26(1):72-79.Xie Li,Yang Hao,Qu Xiaoxia,et al.Characterization of water extractable organic matters from the dominant plants in Lake Dianchi by multiple spectroscopic techniques[J].Research of Environmental Science,2013,26(1):72-79.
[9]陈雪霜,江韬,卢松,等.三峡库区消落带水体DOM不同分子量组分三维荧光特征[J].环境科学,2016,37(3):884-892.Chen Xueshuang,Jiang Tao,Lu Song,et al.Three-dimensional fluorescence spectral characteristics of different molecular weight fractionations of dissolved organic matter in the water-level fluctuation zones of Three Gorges Reservoir Areas[J].Environmental Science,2016,37(3):884-892.
[10]张倩,董靖,吉芳英,等.新建人工深水湖泊沉积物上覆水和孔隙水中溶解性有机质的光谱特征[J].湖泊科学,2018,30(1):112-120.Zhang Qian,Dong Jing,Ji Fangying,et al.Spectral characteristics of dissolved organic matter in overlying water and pore water of newly-built artificial lake sediments[J].Lake Science,2018,30(1):112-120.
[11]JafféR,Boyer J N,Lu X,et al.Source characterization of dissolved organic matter in a subtropical mangrove-dominated estuary by fluorescence analysis[J].Marine Chemistry,2004,84(3/4):195-210.
[12]Ylla I,Anna M Romaní,Sergi Sabater.Labile and recalcitrant organic matter utilization by river biofilm under increasing water temperature[J].Microbial Ecology,2012,64(3):593-604.
[13]Yu G H,Wu M J,Luo Y H,et al.Fluorescence excitationemission spectroscopy with regional integration analysis for assessment of compost maturity[J].Waste Management,2011,31(8):1729-1736.
[14]赵萱.我国不同生态型湖泊沉积物有机质赋存形态及其与重金属相互作用研究[D].济南:山东师范大学,2012.Zhao Xuan.Investigation on Distribution of Organic Matter and Its Complexation with Heavy Metals in Sediments of Different Ecological Lakes in China[D].Jinan:Shandong Normal University,2012.
[15]Hudson N,Baker A,Ward D,et al.Can fluorescence spectrometry be used as a surrogate for the Biochemical Oxygen Demand(BOD)test in water quality assessment-an example from South West England[J].Science of the Total Environment,2008,391(1):149-158.
[16]高洁,江韬,李璐璐,等.三峡库区消落带土壤中溶解性有机质(DOM)吸收及荧光光谱特征[J].环境科学,2015,36(1):151-162.Gao Jie,Jiang Tao,Li Lulu,et al.Ultraviolet-visible(UV-Vis)and fluorescence spectral characteristics of dissolved organic matter(DOM)in soils of water-level fluctuation zones of the Three Gorges Reservoir Region[J].Environmental Science,2015,36(1):151-162.
[17]Ritchelita P Galapate,Aloysius U Baes,Kazuaki Ito,et al.Detection of domestic wastes in Kurose river using synchronous fluorescence spectroscopy[J].Water Research,1998,32(7):2232-2239.
[18]李帅东,姜泉良,黎烨,等.环滇池土壤溶解性有机质(DOM)的光谱特征及来源分析[J].光谱学与光谱分析,2017,37(5):1448-1454.Li Shuaidong,Jiang Quanliang,Li Ye,et al.Spectroscopic characteristics and sources of dissolved organic matter from soils around Dianchi Lake,Kunming[J].Spectroscopy and Spectral Analysis,2017,37(5):1448-1454.
[19]Helms J R,Stubbins A,Ritchie J D,et al.Absorption spectral slopes and slope ratios as indicators of molecular weight,source,and photobleaching of chromophoric dissolved organic matter[J].Limnology&Oceanography,2008,53(3):955-969.
[20]Xiao Y,Sara-Aho T,Hartikainen H,et al.Contribution of ferric iron to light absorption by chromophoric dissolved organic matter[J].Limnology and Oceanography,2013,58(2):653-662.
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