紫外-可见光分光光度法测定八月瓜果皮齐墩果酸
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摘要
为了建立齐墩果酸的紫外光谱分析方法,以香草醛-冰醋酸为显色剂,545nm为测定波长,用标准比较法考察了齐墩果酸含量的紫外光谱分析条件.结果表明,用0.3 mL 5%香草醛-冰醋酸做显色剂,70℃下恒温显色15 min,8.00mg/L齐墩果酸标准溶液的实验测定值为8.02mg/L;齐墩果酸在2.00～16.00mg/L范围内线性关系良好,相关系数为0.999 8.以质量比计,用本方法测定的八月瓜果皮中齐墩果酸含量为3.70 mg/g,平均加标回收率为101.06%,RSD为1.22%;精密度、重复性和稳定性实验的RSD分别为0.02%,0.29%,0.29%.
In order to establish a spectrographic method of measuring oleanolic acid,conditions of UVspectrophotometry analysis was investigated with vanillin-glacial acetic acid as chromogenic reagent and545 nm as the determination wavelength.The results showed that the measured concentration value of 8.00 mg/L oleanolic acid standard solution was 8.02 mg/L with 0.3 mL vanillin-glacial acetic acid as chromogenic agent,duration was 15 min under 70 ℃,linear relation of the oleanolic acid was good in 2.00～16.00 mg/L,and the correlation coefficientwas 0.999 8.Content of oleanolic acid in Akebia trifoliata pericarp was 3.70 mg/g by this analysis method.Standard addition recovery rate was 101.06%,and its relative standard deviation(RSD)was 1.22%.RSD of precision,repeatability and stability was 0.02%,0.29%and 1.29%,respectively.
In order to establish a spectrographic method of measuring oleanolic acid,conditions of UVspectrophotometry analysis was investigated with vanillin-glacial acetic acid as chromogenic reagent and545 nm as the determination wavelength.The results showed that the measured concentration value of 8.00 mg/L oleanolic acid standard solution was 8.02 mg/L with 0.3 mL vanillin-glacial acetic acid as chromogenic agent,duration was 15 min under 70 ℃,linear relation of the oleanolic acid was good in 2.00～16.00 mg/L,and the correlation coefficientwas 0.999 8.Content of oleanolic acid in Akebia trifoliata pericarp was 3.70 mg/g by this analysis method.Standard addition recovery rate was 101.06%,and its relative standard deviation(RSD)was 1.22%.RSD of precision,repeatability and stability was 0.02%,0.29%and 1.29%,respectively.
引文
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[13]郭艳莹,吴勃岩,董静,等.齐墩果酸与环磷酰胺联合应用对H-22荷瘤小鼠P53突变及TNF-α的影响[J].中医药学报,2014(3):32-33.
[14]LEE W,YANG E J,KU S K,et al.Anti-Inflammatory Effects of Oleanolic Acid on LPS-Induced Inflammation In Vitro and In Vivo[J].Inflammation,2013,36(1):94-102.
[15]LI Ying,WANG Jianwei,GU Tieguang,et al.Oleanolic Acid Supplement Attenuates Liquid Fructose-Induced Adipose Tissue Insulin Resistance Through the Insulin Receptor Substrate-1/Phosphatidylinositol 3-Kinase/Akt Signaling Pathway in Rats[J].Toxicology and Applied Pharmacology,2014,277(2):155-163.
[16]TANG Shengnan,HAO Jikui,GAO Dawei,et al.Preparation and Characterization of Oleanolic Acid Nanoparticles[J].Current Pharmaceutical Analysis,2013,9(2):177-182.
[17]GONG Ting,ZHENG Li,ZHEN Xin,et al.Microbial Transformation of Oleanolic Acid by Trichothecium Roseum[J].Journal of Asian Natural Products Research,2014,16(4):383-386.
[18]KIM S H,HONG J H,LEE Y C.Oleanolic acid Suppresses Ovalbumin-Induced Airway Inflammation and Th2-Mediated Allergic Asthma by Modulating the Transcription Factors T-Bet,GATA-3,RORγt and Foxp3in Asthmatic Mice[J].International Immunopharmacology,2014,18(2):311-324.
[19]张秋丰,弓晓杰.齐墩果酸定量分析方法综述[J].大连大学学报,2008,29(6):31-34.
[20]董海丽,纵伟.薄层分离-分光光度法测定木瓜中的齐墩果酸[J].安徽农学通报,2007,13(15):26-91.
[21]姚荣成,田丽红,高云飞,等.高效液相色谱法测定女贞子中齐墩果酸的含量[J].中国医院药学杂志,2007,5(27):704-705.
[22]闫花丽,赵陆华,朱丹妮.衍生化气相色谱法测定夏枯草中齐墩果酸和熊果酸的含量[J].中国中药杂志,1999,24(12):744-746.
[23]张锋,朱培仪,宋粉云.毛细管电泳高频电导法测定夏枯草中齐墩果酸和熊果酸的含量[J].广东药学院学报,2008,2(24):108-111.
[24]邸金明,刘彬彬,赵婧,等.齐墩果酸5种含量测定方法的比较研究[J].西北药学杂志,2013,28(5):483-486.
[25]任亚硕,吴德玲,张伟,等.紫外分光光度法测定醉鱼草果实中总三萜含量[J].安徽中医学院学报,2012,31(5):78-81.
[26]马春梅,王娟.紫外-可见分光光度法测定肝速康片中齐墩果酸含量[J].亚太传统医药,2015,11(16):40-41.
[27]邓世星,孙志勇,周卿,等.紫外光谱法测定齐墩果酸含量[J].遵义医学院学报,2003,26(6):556-557.
[2]班小重,万明长,张朝君,等.野生果树八月瓜的资源收集与利用评价[J].贵州农业科学,2008,36(4):17-19.
[3]张燕君,党海山,杨路路,等.药用植物三叶木通野生资源的地理分布与调查[J].中国野生植物资源,2013,32(3):58-62.
[4]白成科.猫儿屎和三叶木通种子油中脂肪酸成分的GC-MS分析[J].西北植物学报,2007,27(5):1 035-1 038.
[5]欧阳玉祝,张晓旭,唐顼婉,等.八月瓜籽油的特性及营养成分分析[J].中国油脂,2015,40(7):85-88.
[6]马传国,董学工,程亚芳.三叶木通籽成分及三叶木通籽油的理化指标分析[J].中国油脂,2009,34(9):77-79.
[7]孙翔宇,高贵田,严勃,等.三叶木通与猫儿屎种子脂肪酸和氨基酸分析[J].中药材,2012,35(9):1 444-1 447.
[8]欧阳玉祝,李雪峰,姚懿桓.离子沉淀法分离八月瓜果皮中的总多酚[J].食品科学,2014,35(16):76-79.
[9]张孟琴,张丽娜,王朝阳,等.三叶木通果皮总黄酮的提取和含量测定的研究[J].食品工业科技,2010(1):247-250.
[10]田丽婷,马龙,堵年生.齐墩果酸的药理作用研究概况[J].中国中药杂志,2002,27(12):884-887.
[11]董静,吴勃岩,刘艳秋,等.齐墩果酸对H-22荷瘤小鼠肿瘤组织中细胞凋亡相关蛋白表达的影响[J].中国实验方剂学杂志,2015(14):129-131.
[12]LI Qin,FAN Yingsai,GAO Zongqin,et al.Effect of Ursolic Acid and Oleanolic Acid on Osteoblastic Like Cell-Line MC3T3-E1[J].Pakistan Veterinary Journal,2015,35(4):414-419.
[13]郭艳莹,吴勃岩,董静,等.齐墩果酸与环磷酰胺联合应用对H-22荷瘤小鼠P53突变及TNF-α的影响[J].中医药学报,2014(3):32-33.
[14]LEE W,YANG E J,KU S K,et al.Anti-Inflammatory Effects of Oleanolic Acid on LPS-Induced Inflammation In Vitro and In Vivo[J].Inflammation,2013,36(1):94-102.
[15]LI Ying,WANG Jianwei,GU Tieguang,et al.Oleanolic Acid Supplement Attenuates Liquid Fructose-Induced Adipose Tissue Insulin Resistance Through the Insulin Receptor Substrate-1/Phosphatidylinositol 3-Kinase/Akt Signaling Pathway in Rats[J].Toxicology and Applied Pharmacology,2014,277(2):155-163.
[16]TANG Shengnan,HAO Jikui,GAO Dawei,et al.Preparation and Characterization of Oleanolic Acid Nanoparticles[J].Current Pharmaceutical Analysis,2013,9(2):177-182.
[17]GONG Ting,ZHENG Li,ZHEN Xin,et al.Microbial Transformation of Oleanolic Acid by Trichothecium Roseum[J].Journal of Asian Natural Products Research,2014,16(4):383-386.
[18]KIM S H,HONG J H,LEE Y C.Oleanolic acid Suppresses Ovalbumin-Induced Airway Inflammation and Th2-Mediated Allergic Asthma by Modulating the Transcription Factors T-Bet,GATA-3,RORγt and Foxp3in Asthmatic Mice[J].International Immunopharmacology,2014,18(2):311-324.
[19]张秋丰,弓晓杰.齐墩果酸定量分析方法综述[J].大连大学学报,2008,29(6):31-34.
[20]董海丽,纵伟.薄层分离-分光光度法测定木瓜中的齐墩果酸[J].安徽农学通报,2007,13(15):26-91.
[21]姚荣成,田丽红,高云飞,等.高效液相色谱法测定女贞子中齐墩果酸的含量[J].中国医院药学杂志,2007,5(27):704-705.
[22]闫花丽,赵陆华,朱丹妮.衍生化气相色谱法测定夏枯草中齐墩果酸和熊果酸的含量[J].中国中药杂志,1999,24(12):744-746.
[23]张锋,朱培仪,宋粉云.毛细管电泳高频电导法测定夏枯草中齐墩果酸和熊果酸的含量[J].广东药学院学报,2008,2(24):108-111.
[24]邸金明,刘彬彬,赵婧,等.齐墩果酸5种含量测定方法的比较研究[J].西北药学杂志,2013,28(5):483-486.
[25]任亚硕,吴德玲,张伟,等.紫外分光光度法测定醉鱼草果实中总三萜含量[J].安徽中医学院学报,2012,31(5):78-81.
[26]马春梅,王娟.紫外-可见分光光度法测定肝速康片中齐墩果酸含量[J].亚太传统医药,2015,11(16):40-41.
[27]邓世星,孙志勇,周卿,等.紫外光谱法测定齐墩果酸含量[J].遵义医学院学报,2003,26(6):556-557.
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