响应面法优化水蒸气蒸馏提取羌活挥发油
|
摘要
目的:通过响应面优化羌活中α-蒎烯水蒸气蒸馏法的提取条件。方法:采用响应面Box-Behnken设计三因素水平的提取条件进行提取。气相色谱条件:检测器为FID,温度为300℃,进样量为1μL。结果:在浸泡时间80 min,提取时间7 h,液固体积比为10的条件下水蒸气蒸馏法的提取效果最好。结论:响应面设计的方法简便快捷,通过其设计的实验得到结果后处理可以得出科学的结论。
Objective:The extraction conditions of α-pinene steam distillation in Radix Notopterygiiwere optimized by response surface method. Methods:Response surface Box-Behnken was used to design three-factor extraction conditions. Gas chromatography conditions:to FID detector,temperature was 300 ℃,sample quantity for 1 μL. Results:Under the conditions of soaking time of 80 min,extraction time of 7 h and liquid-solid volume ratio of 10,steam distillation had the best extraction effect. Conclusion:The method of response surface design is simple and fast,and the scientific conclusion can be obtained by the postprocessing of the experimental results.
Objective:The extraction conditions of α-pinene steam distillation in Radix Notopterygiiwere optimized by response surface method. Methods:Response surface Box-Behnken was used to design three-factor extraction conditions. Gas chromatography conditions:to FID detector,temperature was 300 ℃,sample quantity for 1 μL. Results:Under the conditions of soaking time of 80 min,extraction time of 7 h and liquid-solid volume ratio of 10,steam distillation had the best extraction effect. Conclusion:The method of response surface design is simple and fast,and the scientific conclusion can be obtained by the postprocessing of the experimental results.
引文
[1]李智勇,张兴水,王军练.羌活的研究进展[J].陕西中医学院学报,2003,26(6):56-59.
[2]李国辉,曾笑,张斌,等.气相色谱-质谱法分析羌活挥发油成分[J].现代中药研究与实践,2007(6):19-22.
[3]吉力,徐植灵,潘炯光,等.羌活挥发油成分分析[J].天然产物研究与开发,1997,9(1):4-8.
[4]胡敏敏,蔡宝昌,张志杰,等.两种方法提取羌活挥发油化学成分的比较[J].中医药学刊,2006,24(10):1931-1932.
[5]匡海学.中药化学[M].北京:中国中医药出版社,2003:213.
[6]刘华钢,陆峥琳,赖茂祥,等.中药挥发油类成分提取分离研究概况[J].辽宁中医药大学学报,2009,11(11):5-7.
[7]LI QH. Application of response surface methodology for extraction optimization of germinant pumpkin seeds protein[J].Food Chemistry,2005,92(4):701-706.
[8]魏安池,代红丽,谷文英.响应面分析法优化红花黄色素提取工艺条件[J].食品与机械,2006,22(2):11-13.
[9]国家药典委员会.中华人民共和国药典[M].北京:中国医药出版社,2015.
[10]秦娇,姚永红,侯智霞,等.响应面分析法优化毛竹叶挥发油提取工艺[J].食品科学,2010,31(6):1-5.
[11]孙哲浩,李宝珍,赵谋明,等.响应面分析法优化荔枝核总黄酮提取工艺的研究[J].食品与机械,2006,22(1):30-32.
[12]张薇,魏翔,谢世清,等.响应面法优化草果挥发油提取工艺条件的筛选研究[J].西部林业科学,2013,42(3):28-32.
[13]王立斌,马永纯,班伟.水蒸气蒸馏法提取薄荷中挥发油[J].通化师范学院学报,2005,26(6):56-57.
[14]邱琴,张国英,刘辛欣,等.超临界CO2流体萃取法与水蒸气蒸馏法提取干姜片挥发油化学成分的比较[J].上海中医药杂志,2005,39(3):54-55.
[15]王万,原红果,陈博,等.中药挥发油研究现状探讨[J].时珍国医国药,2006,17(5):848-850.
[2]李国辉,曾笑,张斌,等.气相色谱-质谱法分析羌活挥发油成分[J].现代中药研究与实践,2007(6):19-22.
[3]吉力,徐植灵,潘炯光,等.羌活挥发油成分分析[J].天然产物研究与开发,1997,9(1):4-8.
[4]胡敏敏,蔡宝昌,张志杰,等.两种方法提取羌活挥发油化学成分的比较[J].中医药学刊,2006,24(10):1931-1932.
[5]匡海学.中药化学[M].北京:中国中医药出版社,2003:213.
[6]刘华钢,陆峥琳,赖茂祥,等.中药挥发油类成分提取分离研究概况[J].辽宁中医药大学学报,2009,11(11):5-7.
[7]LI QH. Application of response surface methodology for extraction optimization of germinant pumpkin seeds protein[J].Food Chemistry,2005,92(4):701-706.
[8]魏安池,代红丽,谷文英.响应面分析法优化红花黄色素提取工艺条件[J].食品与机械,2006,22(2):11-13.
[9]国家药典委员会.中华人民共和国药典[M].北京:中国医药出版社,2015.
[10]秦娇,姚永红,侯智霞,等.响应面分析法优化毛竹叶挥发油提取工艺[J].食品科学,2010,31(6):1-5.
[11]孙哲浩,李宝珍,赵谋明,等.响应面分析法优化荔枝核总黄酮提取工艺的研究[J].食品与机械,2006,22(1):30-32.
[12]张薇,魏翔,谢世清,等.响应面法优化草果挥发油提取工艺条件的筛选研究[J].西部林业科学,2013,42(3):28-32.
[13]王立斌,马永纯,班伟.水蒸气蒸馏法提取薄荷中挥发油[J].通化师范学院学报,2005,26(6):56-57.
[14]邱琴,张国英,刘辛欣,等.超临界CO2流体萃取法与水蒸气蒸馏法提取干姜片挥发油化学成分的比较[J].上海中医药杂志,2005,39(3):54-55.
[15]王万,原红果,陈博,等.中药挥发油研究现状探讨[J].时珍国医国药,2006,17(5):848-850.