脱氮副球菌DYTN-1高效去除污水中的总氮
|
摘要
利用脱氮副球菌的异养硝化和好氧反硝化一体的特点,测定其去除污水中总氮(total nitrogen,TN)的效果。污水中TN在24 h内可被游离状态的脱氮副球菌DYTN-1由100 mg/L降低至20 mg/L以下。为了提高对TN的去除效率,对游离细胞进行了固定化,以不同的二价金属阳离子作为交联剂对脱氮副球菌细胞进行细胞固定化。结果表明:固定化的细胞DYTN-1可以在8 h内使得污水中TN的含量由100 mg/L降低至20 mg/L以下,达到了国家排放标准。正交试验优化后,12批次污水中大部分批次达标所用时间在3 h以内。固定化的最佳条件为:海藻酸钠(sodium alginate,SA)含量为3%,SA与菌种比例为1∶1,氯化钡浓度为0. 1 mol/L,固定化时间为5 h,固定化后的脱氮副球菌DYTN-1可以显著提高废水中的总氮的去除效率。
Biological method is an effective way to remove nitrogen pollutants from wastewater. The efficiency of Paracoccus denitrificans DYTN-1 to remove total nitrogen( TN) from sewage was determined using its characteristics of heterotrophic nitrification and aerobic denitrification. The TN in the sewage could be reduced from 100 mg/L to less than 20 mg/L within 24 h by free P. denitrificans DYTN-1 cells. To improve the removal rate of TN,P. denitrificans DYTN-1 was immobilized by different divalent metal ions as cross-linking agents. The results showed that immobilized DYTN-1 could reduce the TN content in wastewater from 100 mg/L to less than 20 mg/L within 8 h,which met the national emission standard. After optimization,most of 12 batches of wastewater met the standard within 3 h. The optimal condition to immobilize P. denitrificans DYTN-1 was as follows: 3% sodium alginate( SA),the ratio of SA to strain was 1∶ 1,0. 1 mol/L barium chloride,and immobilized for 5 h. In conclusion,immobilized P. denitrificans DYTN-1 can significantly improve its efficiency to remove TN from wastewater.
Biological method is an effective way to remove nitrogen pollutants from wastewater. The efficiency of Paracoccus denitrificans DYTN-1 to remove total nitrogen( TN) from sewage was determined using its characteristics of heterotrophic nitrification and aerobic denitrification. The TN in the sewage could be reduced from 100 mg/L to less than 20 mg/L within 24 h by free P. denitrificans DYTN-1 cells. To improve the removal rate of TN,P. denitrificans DYTN-1 was immobilized by different divalent metal ions as cross-linking agents. The results showed that immobilized DYTN-1 could reduce the TN content in wastewater from 100 mg/L to less than 20 mg/L within 8 h,which met the national emission standard. After optimization,most of 12 batches of wastewater met the standard within 3 h. The optimal condition to immobilize P. denitrificans DYTN-1 was as follows: 3% sodium alginate( SA),the ratio of SA to strain was 1∶ 1,0. 1 mol/L barium chloride,and immobilized for 5 h. In conclusion,immobilized P. denitrificans DYTN-1 can significantly improve its efficiency to remove TN from wastewater.
引文
[1]黄廷林,赵金亚,张海涵,等.一株异养硝化-好氧反硝化菌JY78的筛选及其脱氮特性研究[J].安全与环境学报,2016,16(4):325-331.
[2]余润兰,苗雷.异养硝化细菌Alcaligenes sp. S3除氮特性及动力学[J].环境工程学报,2012,6(3):869-872.
[3] SCHMIDT I,SLIEKERS O,SCHMID M,et al. New concepts of microbial treatment processes for the nitrogen removal in wastewater[J]. Fems Microbiol Rev,2003,27(4):481-492.
[4] STEINLE P,THALMANN P,H HENER P,et al. Effect of environmental factors on the degradation of 2,6-dichlorophenol in soil[J]. Environ Sci Technol,2000,34(5):771-775.
[5]佘健,彭聃.污水新型生物脱氮技术研究综述[J].化工管理,2016(35):228-229.
[6]聂玉华.微曝气强化生态浮床对污水中氮元素的去除效果研究[D].成都:西南交通大学,2015.
[7]赵佩文.不同氮污染水体中微生物对环境的生态响应[D].武汉:华中农业大学,2016.
[8] XU Y,HE T,LI Z,et al. Nitrogen removal characteristics of pseudomonas putida Y-9 capable of heterotrophic nitrification and aerobic denitrification at low temperature[J].Bio Med Research International,2017,17(10):142-148.
[9]程芸.外源信号分子对脱氮副球菌反硝化过程调控作用的研究[D].合肥:中国科学技术大学,2016:16.
[10] ROBERTSONL A,NIEL E W J V,TORREMANS R A M,et al. Simultaneous nitrification and denitrification in aerobic chemostat cultures of Thiosphaera pantotropha[J]. Applied&Environmental Microbiology,1988,18(4):305-315.
[11]彭虹.固定化微生物技术在环境工程中的应用分析[J].资源节约与环保,2017,21(11):63-64.
[12] SANJEEVKUMAR S,NAYAK A S,SANTOSHKUMAR M,et al. Paracoccus denitrificans SD1 mediated augmentation with indigenous mixed cultures for enhanced removal of N,N-dimethylformamide from industrial effluents[J]. Biochem Eng J,2013,79(2):1-6.
[13] DZIEWIT L,DMOWSKI M,BAJ J,et al. Plasmid p AMI2 of Paracoccus aminophilus JCM 7686 carries N,Ndimethylformamide degradation-related genes whose expression is activated by a luxR family regulator[J]. Appl Environ Microbiol,2010,76(6):1 861-1 869.
[14] SARMA S J,PAKSHIRAJAN K. Surfactant aided biodegradation of pyrene using immobilized cells of Mycobacterium frederiksbergense[J]. Int Biodeter Biodegr,2011,65(1):73-77.
[15]陈晓国,陈博,胡帆,等.固定化降解菌去除原水中微囊藻毒素[J].武汉理工大学学报,2011(7):112-115.
[16] PATIL N K,VEERANAGOUDA Y,VIJAYKUMAR M H,et al. Enhanced and potential degradation of ophthalate by Bacillus sp. immobilized cells in alginate and polyurethane[J]. Int Biodeter Biodegr,2006,57(2):82-87.
[17] SASAKI H,NONAKA J,SASAKI T,et al. Ammonia removal from livestock wastewater by ammonia-assimilating microorganisms immobilized in polyvinyl alcohol[J]. J Ind Microbiol Biotechnol,2007,34(2):105-110.
[18] JEONG S K,CHO J S,KONG I S,et al. Purification of aquarium water by PVA gel-immobilized photo synthetic bacteria during goldfish rearing[J]. Biotechnology&Bioprocess Engineering,2009,14(2):238-247.
[19] VEJVODA V,KAPLAN O,KLOZOV J,et al. Mild hydrolysis of nitriles by Fusarium solani strain O1[J]. Folia Microbiol,2006,51(4):251-256.
[20] REBROSM,ROSENBERG M,GROSOV Z,et al. Ethanol production from starch hydrolyzates using Zymomonas mobilis and glucoamylase entrapped in polyvinylalcohol hydrogel[J]. Applied Biochemistry&Biotechnology,2009,158(3):561-570.
[21]左昌平.固定化细胞技术在废水处理中的运用分析[J].环境与发展,2017,29(6):120-121.
[22]赵诣.三株异养硝化细菌的分离、特征及其对水产养殖废水脱氮作用研究[D].杭州:浙江大学,2010.
[23] NEIDHARDT F C,BLOCH P L,SMITH D F. Culture medium for enterobacteria[J]. J Bacteriol,1974,119(3):736-747.
[24]水质总氮的测定碱性过硫酸钾消解紫外分光光度法颁布新标准[J].给水排水,2012(5):139-142.
[25]城镇污水处理厂污染物排放标准[C].稀土环保、放射防护及劳动卫生学术交流会暨稀土环保与新技术论坛,2011.
[26]酒卫敬,汪苹,李奥搏,等.海藻酸钠作为固定化细胞包埋剂的研究[J].科技创新导报,2011(2):12-13.
[2]余润兰,苗雷.异养硝化细菌Alcaligenes sp. S3除氮特性及动力学[J].环境工程学报,2012,6(3):869-872.
[3] SCHMIDT I,SLIEKERS O,SCHMID M,et al. New concepts of microbial treatment processes for the nitrogen removal in wastewater[J]. Fems Microbiol Rev,2003,27(4):481-492.
[4] STEINLE P,THALMANN P,H HENER P,et al. Effect of environmental factors on the degradation of 2,6-dichlorophenol in soil[J]. Environ Sci Technol,2000,34(5):771-775.
[5]佘健,彭聃.污水新型生物脱氮技术研究综述[J].化工管理,2016(35):228-229.
[6]聂玉华.微曝气强化生态浮床对污水中氮元素的去除效果研究[D].成都:西南交通大学,2015.
[7]赵佩文.不同氮污染水体中微生物对环境的生态响应[D].武汉:华中农业大学,2016.
[8] XU Y,HE T,LI Z,et al. Nitrogen removal characteristics of pseudomonas putida Y-9 capable of heterotrophic nitrification and aerobic denitrification at low temperature[J].Bio Med Research International,2017,17(10):142-148.
[9]程芸.外源信号分子对脱氮副球菌反硝化过程调控作用的研究[D].合肥:中国科学技术大学,2016:16.
[10] ROBERTSONL A,NIEL E W J V,TORREMANS R A M,et al. Simultaneous nitrification and denitrification in aerobic chemostat cultures of Thiosphaera pantotropha[J]. Applied&Environmental Microbiology,1988,18(4):305-315.
[11]彭虹.固定化微生物技术在环境工程中的应用分析[J].资源节约与环保,2017,21(11):63-64.
[12] SANJEEVKUMAR S,NAYAK A S,SANTOSHKUMAR M,et al. Paracoccus denitrificans SD1 mediated augmentation with indigenous mixed cultures for enhanced removal of N,N-dimethylformamide from industrial effluents[J]. Biochem Eng J,2013,79(2):1-6.
[13] DZIEWIT L,DMOWSKI M,BAJ J,et al. Plasmid p AMI2 of Paracoccus aminophilus JCM 7686 carries N,Ndimethylformamide degradation-related genes whose expression is activated by a luxR family regulator[J]. Appl Environ Microbiol,2010,76(6):1 861-1 869.
[14] SARMA S J,PAKSHIRAJAN K. Surfactant aided biodegradation of pyrene using immobilized cells of Mycobacterium frederiksbergense[J]. Int Biodeter Biodegr,2011,65(1):73-77.
[15]陈晓国,陈博,胡帆,等.固定化降解菌去除原水中微囊藻毒素[J].武汉理工大学学报,2011(7):112-115.
[16] PATIL N K,VEERANAGOUDA Y,VIJAYKUMAR M H,et al. Enhanced and potential degradation of ophthalate by Bacillus sp. immobilized cells in alginate and polyurethane[J]. Int Biodeter Biodegr,2006,57(2):82-87.
[17] SASAKI H,NONAKA J,SASAKI T,et al. Ammonia removal from livestock wastewater by ammonia-assimilating microorganisms immobilized in polyvinyl alcohol[J]. J Ind Microbiol Biotechnol,2007,34(2):105-110.
[18] JEONG S K,CHO J S,KONG I S,et al. Purification of aquarium water by PVA gel-immobilized photo synthetic bacteria during goldfish rearing[J]. Biotechnology&Bioprocess Engineering,2009,14(2):238-247.
[19] VEJVODA V,KAPLAN O,KLOZOV J,et al. Mild hydrolysis of nitriles by Fusarium solani strain O1[J]. Folia Microbiol,2006,51(4):251-256.
[20] REBROSM,ROSENBERG M,GROSOV Z,et al. Ethanol production from starch hydrolyzates using Zymomonas mobilis and glucoamylase entrapped in polyvinylalcohol hydrogel[J]. Applied Biochemistry&Biotechnology,2009,158(3):561-570.
[21]左昌平.固定化细胞技术在废水处理中的运用分析[J].环境与发展,2017,29(6):120-121.
[22]赵诣.三株异养硝化细菌的分离、特征及其对水产养殖废水脱氮作用研究[D].杭州:浙江大学,2010.
[23] NEIDHARDT F C,BLOCH P L,SMITH D F. Culture medium for enterobacteria[J]. J Bacteriol,1974,119(3):736-747.
[24]水质总氮的测定碱性过硫酸钾消解紫外分光光度法颁布新标准[J].给水排水,2012(5):139-142.
[25]城镇污水处理厂污染物排放标准[C].稀土环保、放射防护及劳动卫生学术交流会暨稀土环保与新技术论坛,2011.
[26]酒卫敬,汪苹,李奥搏,等.海藻酸钠作为固定化细胞包埋剂的研究[J].科技创新导报,2011(2):12-13.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |