基于超声压痕技术的兔眼角膜生物力学特性
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摘要
目的利用超声压痕技术探索兔眼角膜的生物力学特性。方法选取7月龄新西兰白兔眼球7只,制作完整角膜试样并固定于人工前房上。用微量注射泵给人工前房注水改变前房内的压力,并用压力传感器测量;在不同前房压力状态下,利用超声压痕设备在角膜顶点位置进行压痕实验,获得力-位移曲线。研究角膜力学参数(弹性模量、滞回量等)与前房压力的关系。结果由角膜压痕实验所得的力-位移曲线呈现非线性特点。前房内压力越大,角膜的力-位移曲线越陡峭。兔眼角膜的弹性模量随前房压力升高而增大,在前房压力为7～45 mm Hg(1 mm Hg=0. 133 k Pa)范围内,其数值范围为0. 30～1. 55 MPa。随前房压力增大,角膜滞回量呈现线性上升趋势。结论基于超声压痕技术获得的兔眼角膜弹性模量随前房压力呈线性增大,滞回量等角膜黏性特性参数与前房压力也相关。
Objective The biomechanical properties of rabbit corneas in vitro were explored by ultrasonic indentation experiment. Metheds Seven eye balls of New Zealand white rabbit with 7-month-old were used and the corneas were fixed on an artificial anterior chamber. The pressure in the anterior chamber were changed by injecting or ejecting normal saline in the artificial anterior chamber by a microinjection pump,and the intraocular pressure in the chamber was measured in real time by a pressure sensor. Indentation experiment was performed on the corneal apex by the ultrasonic indentation device at different pressure states,and the relationships between corneal biomechanical parameters and intraocular pressure were studied. Results The force-displacement curves of cornea in loading and unloading process were nonlinear. The higher the intraocular pressure,the steeper the load-displacement curves. At the intraocular pressure range of 7 mm Hg to 45 mm Hg,corneal tangent modulus increased with pressure with the value ranging between 0. 30 to 1. 55 MPa. The amount of hysteresis indicated the energy consumption of the cornea during loading and unloading process,was related to the viscous of the cornea. The result showed that the greater the pressure,the greater the amount of hysteresis would be. Conclusions Ultrasonic indentation experiments show that the corneal tangent modulus increases linearly with intraocular pressure and the viscous parameters are related with intraocular pressure.
Objective The biomechanical properties of rabbit corneas in vitro were explored by ultrasonic indentation experiment. Metheds Seven eye balls of New Zealand white rabbit with 7-month-old were used and the corneas were fixed on an artificial anterior chamber. The pressure in the anterior chamber were changed by injecting or ejecting normal saline in the artificial anterior chamber by a microinjection pump,and the intraocular pressure in the chamber was measured in real time by a pressure sensor. Indentation experiment was performed on the corneal apex by the ultrasonic indentation device at different pressure states,and the relationships between corneal biomechanical parameters and intraocular pressure were studied. Results The force-displacement curves of cornea in loading and unloading process were nonlinear. The higher the intraocular pressure,the steeper the load-displacement curves. At the intraocular pressure range of 7 mm Hg to 45 mm Hg,corneal tangent modulus increased with pressure with the value ranging between 0. 30 to 1. 55 MPa. The amount of hysteresis indicated the energy consumption of the cornea during loading and unloading process,was related to the viscous of the cornea. The result showed that the greater the pressure,the greater the amount of hysteresis would be. Conclusions Ultrasonic indentation experiments show that the corneal tangent modulus increases linearly with intraocular pressure and the viscous parameters are related with intraocular pressure.
引文
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[12] Deffieux T,Gennisson JL,Bousquet L,et al. Investigating liver stiffness and viscosity for fibrosis,steatosis and activity staging using shear wave elastography[J]. Journal of Hepatology,2015,62(2):317-324.
[13] Zheng YP,Mak AFT,Lue B. Objective assessment of limb tissue elasticity:development of a manual indentation procedure[J].Journal of Rehabilitation Research&Development,1999,36(2):71-85.
[14] Zheng YP,Choi YKC,Wong K,et al. Biomechanical assessment of plantar foot tissue in diabetic patients using an ultrasound indentation system[J]. Ultrasound in Medicine&Biology,2000,26(3):451-456.
[15] Zheng YP,Leung SF,Mak AFT. Assessment of neck tissue fibrosis using an ultrasound palpation system:a feasibility study[J]. Medical and Biological Engineering and Computing,2000,38(5):497-502.
[16] Wang LK,Huang YP,Tian L,et al. Measurement of corneal tangent modulus using ultrasound indentation[J]. Ultrasonics,2016,71(9):20-28.
[17]张海霞,李林,张昆亚,等.兔眼角膜生物力学特性的年龄相关性[J].医用生物力学,2014,29(3):271-275.Zhang HX,Li L,Zhang KY,et al,Age-related changes in biomechanical properties of rabbit corneas[J]. Journal of Medical Biomechanics,2014,29(3):271-275.
[18]孙太凤,王慧枝,穆晶,等.基于整体角膜膨胀实验对兔眼角膜参数的确定[J].北京生物医学工程,2016,35(2):191-197.Sun TF,Wang HZ,Mu J,et al. Determining the biomechanical properties of rabbit cornea with inflation tests[J]. Beijing Biomedical Engineering,2016,35(2):191-197.
[19]郁继国,包芳军,俞阿勇,等.膨胀法加压兔角膜前表面与后表面所测生物力学性能的比较[J].中华眼视光学与视觉科学杂志,2012,14(5):294-299.Yu JG,Bao FJ,Yu AY,et al. Comparison of biomechanical properties obtained in an inflation test of pressurizing the corneal anterior and posterior surfaces in rabbit[J]. Chinese Journal of Optometry Ophthalmology and Visual Science,2012,14(5):294-299.
[20] Young WC,Budynas RG. Roark’s formulas for stress and strain[M]. New York:Mc Graw-Hill Companies,2002.
[21]连玲艳,宋秀君,张晓融.正常兔角膜生物力学特性和参数的测定[J].中华实验眼科杂志,2012,30(4):346-348.Lian LY,Song XJ,Zhang XR. Biomechanical parameter of normal rabbit cornea[J]. Chinese Journal of Experimental Ophthalmology,2012,30(4):346-348.
[2]姜黎,王勤美,曾衍钧.角膜生物力学性能测量方法的研究进展[J].眼科研究,2009,27(9):824-828.Jiang L,Wang QM,Zen YJ. Current advance on corneal biomechanical parameters measurement[J]. Chinese Ophthalmic Research,2009,27(9):824-828.
[3] Elsheikh A,Anderson K. Comparative study of corneal strip extensometry and inflation test[J]. Journal of the Royal Society Interface,2005,2(3):177-185.
[4]刘志成,张昆亚,王玉慧.兔眼角膜生物力学特性的实验研究[J].中国医学物理学杂志,2003,20(3):189-192.Liu ZC,Zhang KY,Wang YH. The experimental research on biomechanical properties of the rabbit’s corneas[J]. Chinese Journal of Medical Physics,2003,20(3):189-192.
[5]姜黎,包芳军,张东升,等.膨胀试验测定猪眼角膜生物力学参数的研究[J].医用生物力学,2009,24(2):123-126.Jiang L,Bao FJ,Zhang DS,et al. Determining the ex vivo biomechanical properties of porcine cornea with inflation test[J].Journal of Medical Biomechanics,2009,24(2):123-126.
[6] Boschetti F, Triacca V, Spinelli L, et al. Mechanical characterization of porcine corneas[J]. Journal of Biomechanical Engineering,2012,134(3):031003.
[7] Labate C,Lombardo M,De Santo MP,et al. Multiscale investigation of the depth-dependent mechanical anisotropy of the human corneal stroma[J]. Investigative Ophthalmology&Visual Science,2015,56(6):4053-4060.
[8] Khan MA,郝蕴,李贝,等.眼反应分析仪在角膜屈光手术中的应用[J].中国医疗设备,2017,31(3):76-79.Khan MA,Hao Y,Li B,et al. Application of ocular response analyzer in corneal refractive surgery[J]. Chinese Medical Devices,2017,31(3):76-79.
[9]陈昕妍,秦晓,张海霞,等.可视化角膜生物力学分析仪在眼科临床中的应用[J].中国医疗设备,2018,33(7):101-106.Chen XY,Qin X,Zhang HX,et al. Application of visual corneal biomechanical analyzer in ophthalmic clinic[J]. Chinese Medical Devices,2018,33(7):101-106.
[10] Piero DP,Natividad A. Corneal biomechanics:a review[J].Clinical and Experimental Optometry,2015,98(2):107-116.
[11] Masetti O,Hug F,Bouillard K,et al. Characterization of passive elastic properties of the human medial gastrocnemius muscle belly using supersonic shear imaging[J]. Journal of Biomechanics,2012,45(6):978-984.
[12] Deffieux T,Gennisson JL,Bousquet L,et al. Investigating liver stiffness and viscosity for fibrosis,steatosis and activity staging using shear wave elastography[J]. Journal of Hepatology,2015,62(2):317-324.
[13] Zheng YP,Mak AFT,Lue B. Objective assessment of limb tissue elasticity:development of a manual indentation procedure[J].Journal of Rehabilitation Research&Development,1999,36(2):71-85.
[14] Zheng YP,Choi YKC,Wong K,et al. Biomechanical assessment of plantar foot tissue in diabetic patients using an ultrasound indentation system[J]. Ultrasound in Medicine&Biology,2000,26(3):451-456.
[15] Zheng YP,Leung SF,Mak AFT. Assessment of neck tissue fibrosis using an ultrasound palpation system:a feasibility study[J]. Medical and Biological Engineering and Computing,2000,38(5):497-502.
[16] Wang LK,Huang YP,Tian L,et al. Measurement of corneal tangent modulus using ultrasound indentation[J]. Ultrasonics,2016,71(9):20-28.
[17]张海霞,李林,张昆亚,等.兔眼角膜生物力学特性的年龄相关性[J].医用生物力学,2014,29(3):271-275.Zhang HX,Li L,Zhang KY,et al,Age-related changes in biomechanical properties of rabbit corneas[J]. Journal of Medical Biomechanics,2014,29(3):271-275.
[18]孙太凤,王慧枝,穆晶,等.基于整体角膜膨胀实验对兔眼角膜参数的确定[J].北京生物医学工程,2016,35(2):191-197.Sun TF,Wang HZ,Mu J,et al. Determining the biomechanical properties of rabbit cornea with inflation tests[J]. Beijing Biomedical Engineering,2016,35(2):191-197.
[19]郁继国,包芳军,俞阿勇,等.膨胀法加压兔角膜前表面与后表面所测生物力学性能的比较[J].中华眼视光学与视觉科学杂志,2012,14(5):294-299.Yu JG,Bao FJ,Yu AY,et al. Comparison of biomechanical properties obtained in an inflation test of pressurizing the corneal anterior and posterior surfaces in rabbit[J]. Chinese Journal of Optometry Ophthalmology and Visual Science,2012,14(5):294-299.
[20] Young WC,Budynas RG. Roark’s formulas for stress and strain[M]. New York:Mc Graw-Hill Companies,2002.
[21]连玲艳,宋秀君,张晓融.正常兔角膜生物力学特性和参数的测定[J].中华实验眼科杂志,2012,30(4):346-348.Lian LY,Song XJ,Zhang XR. Biomechanical parameter of normal rabbit cornea[J]. Chinese Journal of Experimental Ophthalmology,2012,30(4):346-348.
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