甜叶菊重要农艺性状关联分析
|
摘要
为挖掘与甜叶菊重要农艺性状显著相关标记,利用相关标记辅助甜叶菊育种。本研究对93份甜叶菊种质材料11个重要农艺性状描述性统计,并利用58对EST-SSR引物对其扩增,群体遗传结构分析,基于以上分析采用Tassel 5.0的GLM (generalized linear model)模型进行EST-SSR标记与甜叶菊11个重要农艺性状的关联分析。分析检测到10个标记与6个农艺性状极显著相关,各标记对表型变异的解释率范围为0.071 6～0.189 3,部分标记与2～3个农艺性状极显著关联。
In order to ide ntify the significant agronomic traits related to Stevia rebaudiana Bertoni, the related marker was used to assist stevia breeding. In this study, descriptive statistics of 11 important agronomic traits of 93 Stevia germplasm materials were performed and amplified by 58 pairs of EST-SSR primers. Population genetic structure analysis was performed. Based on the above analysis, Tassel 5.0 GLM(generalized linear model) was used to correlate the 11 important agronomic traits of Stevia rebaudiana with EST-SSR markers. Ten markers were found to be significantly associated with six agronomic traits. The interpretation rate of each marker for phenotypic variation ranged from 0.071 6 to 0.189 3, and some markers might be significantly associated with 2 to 3 agronomic traits.
In order to ide ntify the significant agronomic traits related to Stevia rebaudiana Bertoni, the related marker was used to assist stevia breeding. In this study, descriptive statistics of 11 important agronomic traits of 93 Stevia germplasm materials were performed and amplified by 58 pairs of EST-SSR primers. Population genetic structure analysis was performed. Based on the above analysis, Tassel 5.0 GLM(generalized linear model) was used to correlate the 11 important agronomic traits of Stevia rebaudiana with EST-SSR markers. Ten markers were found to be significantly associated with six agronomic traits. The interpretation rate of each marker for phenotypic variation ranged from 0.071 6 to 0.189 3, and some markers might be significantly associated with 2 to 3 agronomic traits.
引文
He K.Q.,Li Y.Y.,Hu N.B.,Cui G.R.,and Zhou Y.L.,2016,Optimization of DNA extraction method for Stevia rebaudianum berton tissue culture seedlings based on stability of molecular markers,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),14(2):396-402(何克勤,李艳阳,胡能兵,崔广荣,周玉丽,2016,基于分子标记稳定性的甜叶菊组培苗DNA提取方法优化,分子植物育种,14(2):396-402)
Li F.F.,Peng Y.Z.,Xu X.B.,Zi W.,Liu R.,and Liu S.,2016,Rapid determination of Pinus diglucoside and genipinic acid in Eucommia ulmoides with near infrared spectroscopy combined with chemometrics methods,Guangpuxue Yu Guangpu Fenxi(Spectroscopy and Spectral Analysis),36(12):3945-3950(李方飞,彭应枝,许雄博,资文,刘蕊,刘韶,2016,近红外光谱技术结合化学计量学快速测定杜仲中松脂素二葡萄糖苷和京尼平苷酸的含量,光谱学与光谱分析,36(12):3945-3950)
Li G.,2012,NIR spectroscopy model construction and resource screening for nutrition quality characteristics in Stevia rebaudiana,Thesis for M.S.,Zhejiang University,Supervisor Wu J.G.,pp.22-24(李冠,2012,甜叶菊营养品质性状的近红外定标模型的构建及资源筛选,硕士学位论文,浙江大学,导师:吴建国,pp.22-24)
Li Y.T.,2015,Biological characteristics and genetic variation study on diploid and autoteraploid Stevia rebaudianum bertoni,Thesis for M.S.,Nanjing Agricultural University Supervisor:Xiang Z.X.,pp.1-3(李雅婷,2015,甜叶菊二倍体与同源四倍体生物学特性及遗传差异研究,硕士学位论文,南京农业大学,导师:向增旭,pp.1-3)
Liu Z.P.,2015,Genome-wide association studies for 12 agriculture traits in maize and the identification of maize nitrogen response genes,Dissertation for Ph.D.,China Agricultura University,Supervisor:Lai J.S.,pp.4-8(刘志鹏,2015,玉米12个农艺性状的全基因组关联分析及玉米氮响应相关基因的鉴定,博士学位论文,中国农业大学,导师:赖锦盛,pp.4-8)
Luo Q.Y.,Tian M.,Xie Y.S.,Lin Y.Y.,and Wang K.C.,2014Optimization of ultrasonic-assisted ethanol extraction of RAand STV for analysis of steviosides content in Stevia,Zhongguo Tangliao(China Sugar),(2):10-13(罗庆云,田敏,谢越盛,林英英,王康才,2014,甜叶菊甜菊糖苷含量分析用RA和STV乙醇超声提取体系优化,中国糖料,(2):10-13)
Lv L.,Cheng X.W.,Hu N.B.,He K.Q.,and Cui G.R.,2017,Comparative study on photosynthetic characteristics and yield of different strain of Stevia rebaudiana,Anhui Keji Xueyuan Xuebao(Journal of Anhui Science and Technology University),31(6):21-26(吕磊,程晓紊,胡能兵,何克勤,崔广荣2017,不同品系甜叶菊光合特性及产量研究,安徽科技学院学报,31(6):21-26)
Qin H.F.,Long N.,Wu J.G.,and Shi C.H.,2014,Construction of a microsatellite-enriched library and isolation of icrosatellite markers in Stevia rebaudiana,Zuowu Xuebao(Acta Agronomica Sinica),40(3):447-456(秦海峰,龙宁,吴建国,石春海,2014,甜叶菊微卫星富集文库的构建与多态性标记的筛选,作物学报,40(3):447-456)
Shu S.Z.,and Wang W.Z.,1988,The variation of Stevia rebaudiana bertani quantity character and relations to the yield Zuowu Xuebao(Acta Agronomica Sinica),14(2):167-173(舒世珍,王万忠,1988,甜菊数量性状变异及与产量关系,作物学报,14(2):167-173)
Su H.,2016,Association analysis of traits related to tea ouality,Thesis for M.S.,Nanjing Agricultural University,Supervisor:Feng J.C.,pp.5-9(苏会,2016,豫南茶区茶品质相关性状与EST-SSR标记的关联分析,硕士学位论文,河南农业大学,导师:冯建灿,pp.5-9)
Su Y.,2011,Exploitation of EST-SSR markers and construction of genetic maps in flax(Linum usitatissimum L.),Thesis for M.S.,Northeast Agricultural University,Supervisor:Li M.,pp.6-10(苏钰,2011,亚麻(Linum usitatissimum L.)EST-SSR标记开发及遗传图谱构建,硕士学位论文,东北农业大学,导师:李明,pp.6-10)
Wang J.,2013,Large-scale development and application of EST-SSR markers of Liriodendron,Thesis for M.S.,Nanjing Forestry University,Supervisor:Li H.G.,pp.7-9(汪洁,2013,鹅掌楸EST-SSR引物大规模开发及应用,硕士学位论文,南京林业大学,导师:李火根,pp.7-9)
Wang R.G.,2013,The association analysis in F1population of stevia and genentic diversity research on different lines,Thesis for M.S.,College of Agriculture and Biotechnology,Zhejiang University,Supervisor:Wu J.G.,pp.3-43(王瑞果,2013,甜叶菊F1群体关联分析及其不同品系遗传多样性的研究,硕士学位论文,浙江大学,导师:吴建国,pp.3-43)
Yang X.H.,Yan J.B.,Zheng Y.P.,Yu J.M.,and Li J.S.,2007,Reviews of association analtsis for quantitative traits in plants,Zuowu Xuebao(Acta Agronomica Sinica),33(4):523-530(杨小红,严建兵,郑艳萍,余建明,李建生,2007,植物数量性状关联分析研究进展,作物学报,33(4):523-530)
Zhan H.,Wu H.W.,Zhang D.,Liu M.T.,Tang L.Y.,Li H.,Wang Z.J.,Yang B.,Yang L.,Fang J.,and Fu M.H.,2017,Determination of calycosin-7-glucoside and astragaloside in astragali radix with near infrared spectroscopy,Guangpuxue Yu Guangpu Fenxi(Spectroscopy and Spectral Analysis),37(5):1391-1396(战皓,吴宏伟,张东,柳梦婷,唐力英,李化,王祝举,杨滨,杨岚,方婧,付梅红,2017,近红外光谱法测定不同产地黄芪中毛蕊异黄酮葡萄糖苷和黄芪甲苷含量,光谱学与光谱分析,37(5):1391-1396)
Zhang Z.Y.,Huang M.P.,Xu J.M.,Xiang D.Y.,Chen F.F.,and Chang M.S.,2017,Correlation analysis of Leptocybe invasa based on SSR markers,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(4):1660-1666(张照远,黄妹平,徐建民,项东云,陈风帆,常明山,2017,基于SSR标记的桉树枝瘿姬小蜂的关联分析,基因组学与应用生物学,36(4):1660-1666)
Zhu Q.Q.,2011,Construction of molecular genetic linkage map of Stevia rebaudiana,Thesis for M.S.,Zhejiang University,Supervisor:Shi C.H.,pp.3-22(朱琼琼,2011,甜叶菊分子标记遗传连锁图谱的构建,硕士学位论文,浙江大学,导师:石春海,pp.3-22)
Li F.F.,Peng Y.Z.,Xu X.B.,Zi W.,Liu R.,and Liu S.,2016,Rapid determination of Pinus diglucoside and genipinic acid in Eucommia ulmoides with near infrared spectroscopy combined with chemometrics methods,Guangpuxue Yu Guangpu Fenxi(Spectroscopy and Spectral Analysis),36(12):3945-3950(李方飞,彭应枝,许雄博,资文,刘蕊,刘韶,2016,近红外光谱技术结合化学计量学快速测定杜仲中松脂素二葡萄糖苷和京尼平苷酸的含量,光谱学与光谱分析,36(12):3945-3950)
Li G.,2012,NIR spectroscopy model construction and resource screening for nutrition quality characteristics in Stevia rebaudiana,Thesis for M.S.,Zhejiang University,Supervisor Wu J.G.,pp.22-24(李冠,2012,甜叶菊营养品质性状的近红外定标模型的构建及资源筛选,硕士学位论文,浙江大学,导师:吴建国,pp.22-24)
Li Y.T.,2015,Biological characteristics and genetic variation study on diploid and autoteraploid Stevia rebaudianum bertoni,Thesis for M.S.,Nanjing Agricultural University Supervisor:Xiang Z.X.,pp.1-3(李雅婷,2015,甜叶菊二倍体与同源四倍体生物学特性及遗传差异研究,硕士学位论文,南京农业大学,导师:向增旭,pp.1-3)
Liu Z.P.,2015,Genome-wide association studies for 12 agriculture traits in maize and the identification of maize nitrogen response genes,Dissertation for Ph.D.,China Agricultura University,Supervisor:Lai J.S.,pp.4-8(刘志鹏,2015,玉米12个农艺性状的全基因组关联分析及玉米氮响应相关基因的鉴定,博士学位论文,中国农业大学,导师:赖锦盛,pp.4-8)
Luo Q.Y.,Tian M.,Xie Y.S.,Lin Y.Y.,and Wang K.C.,2014Optimization of ultrasonic-assisted ethanol extraction of RAand STV for analysis of steviosides content in Stevia,Zhongguo Tangliao(China Sugar),(2):10-13(罗庆云,田敏,谢越盛,林英英,王康才,2014,甜叶菊甜菊糖苷含量分析用RA和STV乙醇超声提取体系优化,中国糖料,(2):10-13)
Lv L.,Cheng X.W.,Hu N.B.,He K.Q.,and Cui G.R.,2017,Comparative study on photosynthetic characteristics and yield of different strain of Stevia rebaudiana,Anhui Keji Xueyuan Xuebao(Journal of Anhui Science and Technology University),31(6):21-26(吕磊,程晓紊,胡能兵,何克勤,崔广荣2017,不同品系甜叶菊光合特性及产量研究,安徽科技学院学报,31(6):21-26)
Qin H.F.,Long N.,Wu J.G.,and Shi C.H.,2014,Construction of a microsatellite-enriched library and isolation of icrosatellite markers in Stevia rebaudiana,Zuowu Xuebao(Acta Agronomica Sinica),40(3):447-456(秦海峰,龙宁,吴建国,石春海,2014,甜叶菊微卫星富集文库的构建与多态性标记的筛选,作物学报,40(3):447-456)
Shu S.Z.,and Wang W.Z.,1988,The variation of Stevia rebaudiana bertani quantity character and relations to the yield Zuowu Xuebao(Acta Agronomica Sinica),14(2):167-173(舒世珍,王万忠,1988,甜菊数量性状变异及与产量关系,作物学报,14(2):167-173)
Su H.,2016,Association analysis of traits related to tea ouality,Thesis for M.S.,Nanjing Agricultural University,Supervisor:Feng J.C.,pp.5-9(苏会,2016,豫南茶区茶品质相关性状与EST-SSR标记的关联分析,硕士学位论文,河南农业大学,导师:冯建灿,pp.5-9)
Su Y.,2011,Exploitation of EST-SSR markers and construction of genetic maps in flax(Linum usitatissimum L.),Thesis for M.S.,Northeast Agricultural University,Supervisor:Li M.,pp.6-10(苏钰,2011,亚麻(Linum usitatissimum L.)EST-SSR标记开发及遗传图谱构建,硕士学位论文,东北农业大学,导师:李明,pp.6-10)
Wang J.,2013,Large-scale development and application of EST-SSR markers of Liriodendron,Thesis for M.S.,Nanjing Forestry University,Supervisor:Li H.G.,pp.7-9(汪洁,2013,鹅掌楸EST-SSR引物大规模开发及应用,硕士学位论文,南京林业大学,导师:李火根,pp.7-9)
Wang R.G.,2013,The association analysis in F1population of stevia and genentic diversity research on different lines,Thesis for M.S.,College of Agriculture and Biotechnology,Zhejiang University,Supervisor:Wu J.G.,pp.3-43(王瑞果,2013,甜叶菊F1群体关联分析及其不同品系遗传多样性的研究,硕士学位论文,浙江大学,导师:吴建国,pp.3-43)
Yang X.H.,Yan J.B.,Zheng Y.P.,Yu J.M.,and Li J.S.,2007,Reviews of association analtsis for quantitative traits in plants,Zuowu Xuebao(Acta Agronomica Sinica),33(4):523-530(杨小红,严建兵,郑艳萍,余建明,李建生,2007,植物数量性状关联分析研究进展,作物学报,33(4):523-530)
Zhan H.,Wu H.W.,Zhang D.,Liu M.T.,Tang L.Y.,Li H.,Wang Z.J.,Yang B.,Yang L.,Fang J.,and Fu M.H.,2017,Determination of calycosin-7-glucoside and astragaloside in astragali radix with near infrared spectroscopy,Guangpuxue Yu Guangpu Fenxi(Spectroscopy and Spectral Analysis),37(5):1391-1396(战皓,吴宏伟,张东,柳梦婷,唐力英,李化,王祝举,杨滨,杨岚,方婧,付梅红,2017,近红外光谱法测定不同产地黄芪中毛蕊异黄酮葡萄糖苷和黄芪甲苷含量,光谱学与光谱分析,37(5):1391-1396)
Zhang Z.Y.,Huang M.P.,Xu J.M.,Xiang D.Y.,Chen F.F.,and Chang M.S.,2017,Correlation analysis of Leptocybe invasa based on SSR markers,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(4):1660-1666(张照远,黄妹平,徐建民,项东云,陈风帆,常明山,2017,基于SSR标记的桉树枝瘿姬小蜂的关联分析,基因组学与应用生物学,36(4):1660-1666)
Zhu Q.Q.,2011,Construction of molecular genetic linkage map of Stevia rebaudiana,Thesis for M.S.,Zhejiang University,Supervisor:Shi C.H.,pp.3-22(朱琼琼,2011,甜叶菊分子标记遗传连锁图谱的构建,硕士学位论文,浙江大学,导师:石春海,pp.3-22)