猪流行性腹泻毒株S基因分离鉴定及序列分析
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摘要
根据NCBI上传的猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)[GenBank:JN547228(CH/S)],设计引物对阳性病料进行鉴定并回收,与pMD18-T构建重组载体并测序。对猪流行性腹泻病毒的基因多样性及序列保守性进行分析,一是以经典毒株为主,包括CV777、KPEDV-9和CH/S株等为代表的G1基因型,另一种是以近几年分离的毒株为主,包括HB3-CH2012、CXHZ-CH2013、HNCZ-CH2013等为代表的G2基因型。基因同源性分析比较结果表明,这4株PEDV之间的序列同源性为97.6%～100.0%,与经典株CV777的同源性为92.7%～92.8%。同源分析显示,PEDV不同毒株间的基因同源性仍高度相似,但PEDV流行毒株已呈现进化分支的趋势。
引文
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[15]Du L,Zhao G,Kou Z,et al.Identification of a receptor-binding domain in the S protein of the novel human coronavirus Middle East respiratory syndrome coronavirus as an essential target for vaccine development[J].Journal of Virology,2013,87(17):9939-9942.
[16]Gao Y Y,Kou Q W,Ge X N,et al.Phylogenetic analysis of porcine epidemic diarrhea virus field strains prevailing recently in China[J].Archives of Virology,2013,158(3):711-715.
[17]Belouzard S,Millet J K,Licitra B N,et al.Mechanisms of coronavirus cell entry mediated by the viral spike protein[J].Viruses,2012,4(6):1011-1013.吴晓军,胡喜贵,陈向东,等.小麦成熟胚再生体系优化效果评价及高再生率基因型筛选[J].江苏农业科学,2019,47(11):74-77.
[2]Pan Y,Tian X,Li W,et al.Isolation and characterization of a variant porcine epidemic diarrhea virus in China[J].Virology Journal,2012:195.
[3]Song D,Park B.Porcine epidemic diarrhoea virus:a comprehensive review of molecular epidemiology,diagnosis,and vaccines[J].Virus Genes,2012,44(2):167-175.
[4]Chen J,Wang C,Shi H,et al.Molecular epidemiology of porcine epidemic diarrhea virus in China[J].Archives of Virology,2010,155:1471-1476.
[5]Park J E,Cruz D J,Shin H J.Receptor-bound porcine epidemic diarrhea virus spike protein cleaved by trypsin induces membrane fusion[J].Archives of Virology,2011,156:1749-1756.
[6]Chen J F,Liu X Z,Shi D,et al.Detection and molecular diversity of spike gene of porcine epidemic diarrhea virus in China[J].Viruses,2013,5(10):2601-2613.
[7]Hao J,Xue C,He L,et al.Bioinformatics insight into the spike glycoprotein gene of field porcine epidemic diarrhea strains during 2011—2013 in Guangdong,China[J].Virus Genes,2014,49(1):58-67.
[8]Tian P F,Jin Y L,Xing G,et al.Evidence of recombinant strains of porcine epidemic diarrhea virus,United States,2013[J].Emerging Infectious Diseases,2014,20(10):1735-1738.
[9]Li Z L,Zhu L,Ma J Y,et al.Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field strains in South China[J].Virus Genes,2012,45(1):181-185.
[10]田野,苏丹萍,蒋伟,等.华南地区猪流行性腹泻病毒的分离鉴定及其S基因抗原表位片段的遗传变异分析[J].中国兽医杂志,2013,49(5):3-5,8.
[11]孙东波,陈建飞,时洪艳,等.猪流行性腹泻病毒S蛋白受体结合域的分析[J].畜牧兽医学报,2009,40(4):528-532.
[12]Sun D B,Feng L,Shi H Y,et al.Spike protein region (aa 636-789) of Porcine epidemic diarrhea virus is essential for induction of neutralizing antibodies[J].Acta Virologica,2007,51(3):149-156.
[13]Sun D B,Feng L,Shi H Y,et al.Identification of two novel B cell epitopes on porcine epidemic diarrhea virus spike protein[J].Veterinary Microbiology,2008,131(1/2):73-81.
[14]郑逢梅,霍金耀,赵军,等.2010—2012年华中地区猪流行性腹泻病毒分子特征和遗传进化分析[J].病毒学报,2013,29(2):197-205.
[15]Du L,Zhao G,Kou Z,et al.Identification of a receptor-binding domain in the S protein of the novel human coronavirus Middle East respiratory syndrome coronavirus as an essential target for vaccine development[J].Journal of Virology,2013,87(17):9939-9942.
[16]Gao Y Y,Kou Q W,Ge X N,et al.Phylogenetic analysis of porcine epidemic diarrhea virus field strains prevailing recently in China[J].Archives of Virology,2013,158(3):711-715.
[17]Belouzard S,Millet J K,Licitra B N,et al.Mechanisms of coronavirus cell entry mediated by the viral spike protein[J].Viruses,2012,4(6):1011-1013.吴晓军,胡喜贵,陈向东,等.小麦成熟胚再生体系优化效果评价及高再生率基因型筛选[J].江苏农业科学,2019,47(11):74-77.