花斑皮蠹线粒体基因组序列测定与分析
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摘要
花斑皮蠹(Trogoderma variabile)隶属于鞘翅目(Coleoptera)皮蠹科(Dermestidae),是一种世界性分布的具有重要经济意义的有害生物,我国口岸常有截获。本研究用二代测序方法测定了花斑皮蠹的线粒体基因组序列。结果表明花斑皮蠹线粒体基因组全长15 594 bp(GenBank登录号:MH922966),包含13个蛋白质编码基因、22个转运RNA基因、2个核糖体RNA基因和1个控制区,基因排列顺序与已知的白腹皮蠹(Dermestes maculatus)、小圆皮蠹(Dermestes verbasci)线粒体基因组序列一致;线粒体基因组的A+T含量为71.0%;13个蛋白质编码基因的起始密码子均为ATN,终止密码子除nad4以不完全的T作为终止密码子外,其余的基因均以典型的TAA或TAG作为终止密码子;22个tRNA中,除tRNA~(Ser(AGN))的DHU臂缺失外,其他tRNAs均能折叠成典型的三叶草结构。本文首次测定了花斑皮蠹的线粒体基因组序列,由于皮蠹科线粒体基因组数据有限,未开展系统发育分析。
Trogoderma variabile(Coleoptera:Dermestidae) is a world-wide pest with important economic significance and intercepted frequently at China ports. In this study,the next-generation sequencing was used to get the complete mitochondrial genome of T. variabile. The results showed that the mitochondrial genome of T. variabile was 15 594 bp(GenBank accession no.:MH922966) in length,which contained 13 Protein-coding genes,22 translocation RNA genes,2 ribosomal RNA genes and one control region. The structure and organization of the molecule were similar with the published sequences of Dermestes maculatus and Dermeste verbasci. The A+T content of the whole mitochondrial genome of T. variabile was71.0%. The start codons of 13 protein-coding genes were ATN,except that nad4 took incomplete T as the stop codon,all the other genes used the typical TAA or TAG as the stop codon. In 22 tRNAs,only tRNASer(AGN)could not folded into a typical clover structure for lack of DHU arm. It is the first report of complete mitochondrial genome of T. variabile. However,restricted by the number of mitochondrial genome sequences of Dermestidae,the phylogenetic analysis is not proceeded.
Trogoderma variabile(Coleoptera:Dermestidae) is a world-wide pest with important economic significance and intercepted frequently at China ports. In this study,the next-generation sequencing was used to get the complete mitochondrial genome of T. variabile. The results showed that the mitochondrial genome of T. variabile was 15 594 bp(GenBank accession no.:MH922966) in length,which contained 13 Protein-coding genes,22 translocation RNA genes,2 ribosomal RNA genes and one control region. The structure and organization of the molecule were similar with the published sequences of Dermestes maculatus and Dermeste verbasci. The A+T content of the whole mitochondrial genome of T. variabile was71.0%. The start codons of 13 protein-coding genes were ATN,except that nad4 took incomplete T as the stop codon,all the other genes used the typical TAA or TAG as the stop codon. In 22 tRNAs,only tRNASer(AGN)could not folded into a typical clover structure for lack of DHU arm. It is the first report of complete mitochondrial genome of T. variabile. However,restricted by the number of mitochondrial genome sequences of Dermestidae,the phylogenetic analysis is not proceeded.
引文
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[16]Peng Y,Leung H C M,Yiu S M,et al.IDBA-UD:a de novo assembler for single-cell and metagenomic sequencing data with highly uneven depth.Bioinformatics,2012,28(11):1420-1428.
[17]Kearse M,Moir R,Wilson A,et al.Geneious Basic:An integrated and extendable desktop software platform for the organization and analysis of sequence data.Bioinformatics,2012,28(12):1647-1649.
[18]Kumar S,Stecher G,Tamura K.MEGA7:Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets.Molecular Biology&Evolution,2016,33(7):1870.
[19]Sheffield N C,Song H,Cameron S L,et al.Nonstationary evolution and compositional heterogeneity in beetle mitochondrial phylogenomic.Systematic Biology,2009,58(4):381-394.
[20]林爱丽,李欣欣,赵新成.黑毛皮蠹线粒体基因组分析及皮蠹科系统发育分析.昆虫学报,2018,61(4):477-487.
[21]聂瑞娥,杨星科.鞘翅目昆虫线粒体基因组研究进展.昆虫学报,2014,57(7):860-868.
[22]Clary D O,Wolstenholme D R.The mitochondrial DNAmolecular of Drosophila yakuba:nucleotide sequence,gene organization,and genetic code.Journal of Molecular Evolution,1985,22(3):252-271.
[2]Kiselyova T,Mchugh J V.A phylogenetic study of Dermestidae(Coleoptera)based on larval morphology.Systematic Entomology,2010,31(3):469-507.
[3]Mroczkowski M.Distribution of the Dermestidae(Coleoptera)of the world with a catalogue of all known species.Annales Zoologici,1968,26(3):15-191.
[4]赵养昌,李鸿兴,高锦亚.中国仓库害虫.北京:农业出版社,1982:57.
[5]张生芳,施宗伟,薛光华,等.储藏物甲虫鉴定.北京:中国农业科学技术出版,2004:80-82.
[6]凤舞剑,戴优强,胡长效.花斑皮蠹的生物学特性及防治技术.安徽农业科学,2004,32(3):472-473.
[7]Burges H D.Studies on the Dermestid beetle Trogoderma granarium Everts.IV.Feeding,growth,and respiration with particular reference to diapause larvae.Journal of Insect Physiology,1960,5(3):317-334.
[8]张生芳,樊新华,高渊,等.储藏物甲虫.北京:科学出版社,2016:134.
[9]Wei C Y,Ren B Z,Chen X,et al.Scanning Electron Microscope Observations on the Antennal Sensilla of Two Stored Grain Pests Trogoderma granarium and Trogoderma variabile(Coleoptera:Dermestidae).Florida Entomologist,2015,98(1):140-148.
[10]Zhao Y C,Li H X.A study of Chinese Trogoderma Berthold(Coleoptera,Dermestidae).Acta Zootaxonomica Sinica,1966,3:245-254.
[11]Brown W M,George M Jr,Wilson A C.Rapid evolution of animal mitochondrial DNA.Proceedings of the National Academy of Sciences of the United States of America,1979,76(4):1967-1971.
[12]Ballard J W,Whitlock M C.The incomplete natural history of mitochondria.Molecular Ecology,2004,13(4):729-744.
[13]Boore J L.Animal mitochondrial genomes.Nucleic Acids Res,1999,27(8):1767-1780.
[14]Bae J S,Kim I,Sohn H D,et al.The mitochondrial genome of the firefly,Pyrocoelia rufa:complete DNA sequence,genome organization,and phylogenetic analysis with other insects.Molecular Phylogenetics&Evolution,2004,32(3):978-985.
[15]Cameron S L.Insect mitochondrial genomics:implications for evolution and phylogeny.Annual Review of Entomology,2014,59(1):95-117.
[16]Peng Y,Leung H C M,Yiu S M,et al.IDBA-UD:a de novo assembler for single-cell and metagenomic sequencing data with highly uneven depth.Bioinformatics,2012,28(11):1420-1428.
[17]Kearse M,Moir R,Wilson A,et al.Geneious Basic:An integrated and extendable desktop software platform for the organization and analysis of sequence data.Bioinformatics,2012,28(12):1647-1649.
[18]Kumar S,Stecher G,Tamura K.MEGA7:Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets.Molecular Biology&Evolution,2016,33(7):1870.
[19]Sheffield N C,Song H,Cameron S L,et al.Nonstationary evolution and compositional heterogeneity in beetle mitochondrial phylogenomic.Systematic Biology,2009,58(4):381-394.
[20]林爱丽,李欣欣,赵新成.黑毛皮蠹线粒体基因组分析及皮蠹科系统发育分析.昆虫学报,2018,61(4):477-487.
[21]聂瑞娥,杨星科.鞘翅目昆虫线粒体基因组研究进展.昆虫学报,2014,57(7):860-868.
[22]Clary D O,Wolstenholme D R.The mitochondrial DNAmolecular of Drosophila yakuba:nucleotide sequence,gene organization,and genetic code.Journal of Molecular Evolution,1985,22(3):252-271.
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