结扎大鼠经脉对脏器形态与系数影响的初步观察
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摘要
目的探索结扎法堵塞循经低流阻通道,制造经脉不通大鼠模型的方法,对大鼠的脏器形态与系数的影响。方法将31只雄性SD大鼠随机分为空白对照组10只,胃经结扎组10只和肾经结扎组11只。使用线结扎法阻断大鼠的胃经和肾经低流阻通道,制造经脉不通模型,观察造模后第7周大鼠脏器系数及脏器剖检情况。结果与对照组比较,胃经结扎组心脏系数、肾脏系数显著升高(P<0.05);肾经结扎组肾上腺系数显著升高(P<0.01),附睾系数显著降低(P<0.01)和前列腺系数显著降低(P<0.05)。解剖肉眼观察发现,胃经结扎组心脏、肾脏、睾丸明显形态大小异常,腹部异常血肿物;肾经结扎组精囊、附睾和盲肠出现异常,1例胸腹腔脏器结构异常。结论结扎法制备经脉不通病理模型可对大鼠相关脏器产生影响,结扎不同经脉出现不同的脏器异常,部分验证了经脉与脏腑之间的关系。
Objective To explore the method of blocking the low flow hydraulic resistance channel along meridians with ligation in rats and to make blocked-menidian model observe the effects on the morphology and organ coefficients in rats.Methods 31 male SD rats were randomly divided into 3 groups(Control group[CG] =10,Ligation on The Stomach Meridian group[LSTMG] =10,Ligation on The Kidney Meridian group[LKIMG] =11).A pathological situation of blocked meridian was made by ligation of the low hydraulic resistance channels along stomach meridian and kidney meridian.The viscera coefficient and the situation of dissection were observed in the seventh week after channel blocked.Results Compared with CG,the cardiac and renal coefficient of the STMG were increased significantly(P<0.05) and the adrenal coefficient of the KIMG was also increased significantly(P<0.01).The epididymis coefficient of the KIMG was decreased significantly(P<0.01) and prostate coefficient showed the same changes(P<0.05).In the gross anatomy observation,the shape and size of heart,kidney and testicles presented abnormal,abdominal hematoma was discovered in the STMG.A rat in the KIMG presented abnormal structure of the main viscera while other rats mainly changed in the seminal vesicle,epididymis and caecum.Conclusion The pathological model of blocked meridian established by ligating has an effect on the main organs of rats.This finding further validates the relationship between meridians and viscera.
Objective To explore the method of blocking the low flow hydraulic resistance channel along meridians with ligation in rats and to make blocked-menidian model observe the effects on the morphology and organ coefficients in rats.Methods 31 male SD rats were randomly divided into 3 groups(Control group[CG] =10,Ligation on The Stomach Meridian group[LSTMG] =10,Ligation on The Kidney Meridian group[LKIMG] =11).A pathological situation of blocked meridian was made by ligation of the low hydraulic resistance channels along stomach meridian and kidney meridian.The viscera coefficient and the situation of dissection were observed in the seventh week after channel blocked.Results Compared with CG,the cardiac and renal coefficient of the STMG were increased significantly(P<0.05) and the adrenal coefficient of the KIMG was also increased significantly(P<0.01).The epididymis coefficient of the KIMG was decreased significantly(P<0.01) and prostate coefficient showed the same changes(P<0.05).In the gross anatomy observation,the shape and size of heart,kidney and testicles presented abnormal,abdominal hematoma was discovered in the STMG.A rat in the KIMG presented abnormal structure of the main viscera while other rats mainly changed in the seminal vesicle,epididymis and caecum.Conclusion The pathological model of blocked meridian established by ligating has an effect on the main organs of rats.This finding further validates the relationship between meridians and viscera.
引文
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[9] 周文婷. 堵塞胃经低流阻通道对小型猪肠胃与行为学影响的研究[D]. 北京:中国中医科学院, 2014.Zhou WT. The study on the influences of gastrointestine and behavioristics after blocking low hydraulic resistance channel along the stomach meridian in mini-pigs[D]. Beijing: China Academy of Chinese Medical Sciences, 2014.
[10] 李宏彦,王燕平,张维波,等.小型猪经脉不通病理模型的宏观定量观察法[J].中国中医基础医学杂志, 2016,22(4):525-528. Li HY, Wang YP, Zhang WB, et al. Macroscopical and quantitative observation method of pathological changes in minipigs after blocking channels[J]. Journal of Basic Chinese Medicine, 2016,22(4):525-528.
[11] 黄琰,王燕平,张维波,等.使用结扎法建立大鼠经脉不通病理模型的初步研究[J].中国中医基础医学杂志, 2018,24(5):592-595. Huang Y, Wang YP, Zhang WB, et al. Primary research of the establishment of a pathological model of rat meridians obstruction by ligation[J]. Journal of Basic Chinese Medicine, 2018,24(5):592-595.
[12] 熊鹰,蔡文琴,李希成,等.大鼠不同类型学习记忆的发育变化研究[J].中国行为医学科学, 1998,7(4):243-245.Xiong Y, Cai WQ, Li XC, et al. Developmental changes of learning and memory in rats[J]. Chinese Journal of Behavioral Medicine Science, 1998,7(4):243-245.
[13] 黄龙祥. 中国针灸学术史大纲[M].北京:华夏出版社,2001:392-394. Huang LX. The historical development of acupuncture in China[M]. Beijing: Huaxia Publishing House,2001:392-394.
[14] 马晓彤,刘燕池,吕英华.经络脏腑相关联系的定位、定性与定量研究[J].复杂系统与复杂性科学, 2004,1(1):58-67. Ma XT, Liu YC, Lyu YH. Research on relationship between meridians and organs with positive methods of fixed position, determining the nature and quantitative analysis[J]. Complex Systems and Complexity Science, 2004,1(1):58-67.
[15] 严鸿丽,焦琳,熊俊,等.针刺治疗功能性消化不良随机对照试验腧穴谱研究[J].江西中医药大学学报, 2017,29(1):64-66.Yan HL, Jiao L, Xiong J, et al. Acupuncture treatment of functional dyspepsia randomized controlled trials of acupoint spectrum[J]. Journal of Jiangxi University of TCM, 2017,29(1):64-66.
[2] Zhang WB, Jia DX, Li HY, et al. Understanding qi running in the meridians as interstitial fluid flowing via interstitial space of low hydraulic resistance[J]. Chinese Journal of Integrative Medicine, 2018,24(4):304-307.
[3] 张维波,田宇瑛,李宏,等. 一种连续测量流阻的方法与皮下低流阻点的研究[J]. 生物物理学报, 1998,14(2):373-379.Zhang WB, Tian YY, Li H, et al. A method to measure hydraulic resistence of subcutis continuously and the study of low hydraulic resistance points [J]. Acta Biophysica Sinica, 1998,14(2):373-379.
[4] Zhang WB, Tian YY, Li H, et al. A discovery of low hydraulic resistance channel along meridians[J]. Journal of Acupuncture and Meridian Studies, 2008, 1(1):20-28.
[5] Zhang WB, Wang GJ, Fuxe K. Classic and modern meridian studies: a review of low hydraulic resistance channels along meridians and their relevance for therapeutic effects in traditional Chinese medicine[J]. Evidence-Based Complementray and Alternative Medicine, 2015(3):410979.
[6] 马迎民,徐德成,范吉平.“脏腑之精化生脏腑之气”的现代医学机制[J].中医杂志, 2017,58(12):996-1001. Ma YM,Xu DC,Fan JP. Modern medicine mechanism of “essence of zang-fu organs turning into qi of zang-fu organs”[J]. Journal of Traditional Chinese Medicine, 2017,58(12):996-1001.
[7] 张栋,王淑友,马惠敏,等.内脏病变(心包炎)体表循经温度反应的红外热像图显示[J].中国中医基础医学杂志, 2004,10(7):52-54. Zhang D, Wang SY, Ma HM, et al. Infrared thermography of temperature response along meridians on the surface of visceral lesions (pericarditis)[J]. Journal of Basic Chinese Medicine, 2004,10(7):52-54.
[8] 张宇沁. 通过行为学观察研究小型猪经脉不通病理模型的方法学探索[D].北京:中国中医科学院,2011.Zhang YQ. Methodological study on behavioral observation in pathological model of meridian blockage in Mini-pigs[D]. Beijing: China Academy of Chinese Medical Sciences, 2011.
[9] 周文婷. 堵塞胃经低流阻通道对小型猪肠胃与行为学影响的研究[D]. 北京:中国中医科学院, 2014.Zhou WT. The study on the influences of gastrointestine and behavioristics after blocking low hydraulic resistance channel along the stomach meridian in mini-pigs[D]. Beijing: China Academy of Chinese Medical Sciences, 2014.
[10] 李宏彦,王燕平,张维波,等.小型猪经脉不通病理模型的宏观定量观察法[J].中国中医基础医学杂志, 2016,22(4):525-528. Li HY, Wang YP, Zhang WB, et al. Macroscopical and quantitative observation method of pathological changes in minipigs after blocking channels[J]. Journal of Basic Chinese Medicine, 2016,22(4):525-528.
[11] 黄琰,王燕平,张维波,等.使用结扎法建立大鼠经脉不通病理模型的初步研究[J].中国中医基础医学杂志, 2018,24(5):592-595. Huang Y, Wang YP, Zhang WB, et al. Primary research of the establishment of a pathological model of rat meridians obstruction by ligation[J]. Journal of Basic Chinese Medicine, 2018,24(5):592-595.
[12] 熊鹰,蔡文琴,李希成,等.大鼠不同类型学习记忆的发育变化研究[J].中国行为医学科学, 1998,7(4):243-245.Xiong Y, Cai WQ, Li XC, et al. Developmental changes of learning and memory in rats[J]. Chinese Journal of Behavioral Medicine Science, 1998,7(4):243-245.
[13] 黄龙祥. 中国针灸学术史大纲[M].北京:华夏出版社,2001:392-394. Huang LX. The historical development of acupuncture in China[M]. Beijing: Huaxia Publishing House,2001:392-394.
[14] 马晓彤,刘燕池,吕英华.经络脏腑相关联系的定位、定性与定量研究[J].复杂系统与复杂性科学, 2004,1(1):58-67. Ma XT, Liu YC, Lyu YH. Research on relationship between meridians and organs with positive methods of fixed position, determining the nature and quantitative analysis[J]. Complex Systems and Complexity Science, 2004,1(1):58-67.
[15] 严鸿丽,焦琳,熊俊,等.针刺治疗功能性消化不良随机对照试验腧穴谱研究[J].江西中医药大学学报, 2017,29(1):64-66.Yan HL, Jiao L, Xiong J, et al. Acupuncture treatment of functional dyspepsia randomized controlled trials of acupoint spectrum[J]. Journal of Jiangxi University of TCM, 2017,29(1):64-66.
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