结缕草新品种DUS测试数量性状特异性判别
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摘要
DUS测试是植物新品种保护的技术基础和授权依据。本研究按照农业部发布的结缕草属(Zoysia)DUS测试指南,对包括两个申请品种及其近似品种和标准品种在内的10份结缕草材料进行了连续两个独立生长周期的DUS测试,并使用代码法和数理统计分析法两种方法对8个数量性状的特异性进行判定,旨在为结缕草申请品种DUS数量性状的特异性判定提供依据。两种方法判定结果比较发现,使用代码法判定有特异性的数量性状不一定在统计学上有显著差异,而判定为没有特异性的数量性状在统计学上却可能有显著差异。为了避免特异性误判,对代码法判定有特异性的数量性状进一步做了数理统计分析。结果表明,广绿结缕草在匍匐茎节间长、叶长和种子千粒重3个数量性状上具备特异性;Z0413-1结缕草在匍匐茎节间长、叶宽和花序小穗数3个数量性状上具备特异性。
DUS testing provides a technical and authoritative basis for the protection of new plant varieties.Ten zoysiagrass varieties,including two candidate varieties,two similar varieties,and standard varieties,were selectedin the present study for DUS testing over two consecutive growing seasons.The test was strictly guided by DUS testing guidelines,published by the Ministry of Agriculture of China.The distinctness of eight quantitative characteristics were assessed by two methods(note and statistical analysis)to provide practical evidence for the assessment of quantitative characteristics of zoysiagrass DUS testing.The assessment of results from the two methods showed that quantitative characteristics with distinctness that were assessed by the notemethod showed non-significant differences by the statistical analysis method,whereas characteristics without distinctness showed significant differences by the statistical analysis method.To avoid such misjudgment of distinctness,the quantitative characteristics with distinctness assessed by the notemethod were further analyzed with the statistical analysis method.The results showed that the candidate variety of zoysiagrass Guanglühad distinctness in quantitative characteristics of stolon length of internode,leaf length,and seed kilo grain weight,whereas the candidate variety of zoysiagrass Z0413-1 had distinctness in quantitative characteristics of stolon length of internode,leaf width,and inflorescence numbers of spikelet.
DUS testing provides a technical and authoritative basis for the protection of new plant varieties.Ten zoysiagrass varieties,including two candidate varieties,two similar varieties,and standard varieties,were selectedin the present study for DUS testing over two consecutive growing seasons.The test was strictly guided by DUS testing guidelines,published by the Ministry of Agriculture of China.The distinctness of eight quantitative characteristics were assessed by two methods(note and statistical analysis)to provide practical evidence for the assessment of quantitative characteristics of zoysiagrass DUS testing.The assessment of results from the two methods showed that quantitative characteristics with distinctness that were assessed by the notemethod showed non-significant differences by the statistical analysis method,whereas characteristics without distinctness showed significant differences by the statistical analysis method.To avoid such misjudgment of distinctness,the quantitative characteristics with distinctness assessed by the notemethod were further analyzed with the statistical analysis method.The results showed that the candidate variety of zoysiagrass Guanglühad distinctness in quantitative characteristics of stolon length of internode,leaf length,and seed kilo grain weight,whereas the candidate variety of zoysiagrass Z0413-1 had distinctness in quantitative characteristics of stolon length of internode,leaf width,and inflorescence numbers of spikelet.
引文
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[9]徐振江,刘洪,李春兰,李之林,任永浩.水稻新品种DUS测试数量性状特异性统计分析判别研究.华南农业大学学报,2008,29(1):6-9.Xu Z J,Liu H,Li C L,Li Z L,Ren Y H.Study on the statistical assessment of distinctness of quantitative characteristics in DUS testing of new rice varieties.Journal of South China Agriculture University,2008,29(1):6-9.(in Chinese)
[10]陈海荣,顾晓君.水稻新品种测试中性状差异显著性判定的分析.上海农业学报,2003,19(4):18-20.Chen H R,Gu X J.Analysis on the significance of the difference of traits in new rice varieties.Acta Agriculturae Shanghai,2003,19(4):18-20.(in Chinese)
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[12]UPOV.Document TGP/8/1(draft1):Use of statistical procedures indistinctness,uniformity and stability testing,[2016-10-28].http://www.upov.int/edocs/tgpdocs/en/tgp_8.pdf
[13]李汝玉,宋国安,杨平平.利用SSR标记进行品种权有争议品种的鉴定.种子,2000(1):66-67.Li R Y,Song G A,Yang P P.Identification of varieties with disputed rights using SSR Markers.Seed,2000(1):66-67.(in Chinese)
[14]张清华.利用多重荧光SSR标记构建甘蓝型油菜品种的DNA指纹图谱.武汉:华中农业大学硕士学位论文,2011.Zhang Q H,Construction of DNA fingerprinting in rapeseed varieties(B.napus L.)using multiplexed fluorescent SSR markers.Master Thesis.Wuhan:Huazhong Agricultural University,2011.(in Chinese)
[15]王立新,常利芳,李宏博,季伟,刘丽华,赵昌平.小麦区试品系DUS测试的分子标记.作物学报,2010,36(7):1114-1125.Wang L X,Chang L F,Li H B,Ji W,Liu L H,Zhao C P.Molecularmarkers for estimating distinctness,uniformity and stability of wheat lines in regional trials.Acta Agrononica Sinica,2010,36(7):1114-1125.(in Chinese)
[16]王艳平,沈奇,张继红,李华勇,吴燕.大麦DUS测试标准品种的遗传多样性分析及指纹图谱的构建.麦类作物学报,2013,33(2):273-278.Wang Y P,Shen Q,Zhang J H,Li H Y,Wu Y.Genetic diversity analysis and building of DNA fingerprints of barley standard varieties in DUS testing based on SSR markers.Journal of Triticeae Crops,2013,33(2):273-278.(in Chinese)
[17]范建光,张海英,宫国义,肖菁,郭绍贵,任毅,张洁,王秀峰,马艳明,许勇.西瓜DUS测试标准品种SSR指纹图谱构建及应用.植物遗传资源学报,2013,14(5):892-899.Fang J G,Zhang H Y,Gong G Y,Xiao J,Guo S G,Ren Y,Zhang J,Wang X F,Ma Y M,Xu Y.Construction and application of SSR fingerprint database of the example varieties in watermelon DUS testing.Journal of Plant Genetic Resources,2013,14(5):892-899.(in Chinese)
[18]丁西朋,张龙,罗小燕,严琳玲,刘国道,白昌军.柱花草DUS测试标准品种DNA指纹图谱构建.草业科学,2015,32(12):2047-2056.Ding X P,Zhang L,Luo X Y,Yan L L,Liu G D,Bai C J.Construction of DNA fingerprinting of Stylosanthes standard cultivars for DUS testing.Pratacultural Science,2015,32(12):2047-2056.(in Chinese)
[19]杨盛婷,武炳超,冯芹,黄秀,张新全,凌瑶,黄婷,黄琳凯.牛鞭草属DUS测试材料的SSR指纹图谱构建.分子植物育种,2017,15(5):1790-1799.Yang S T,Wu B C,Feng Q,Huang X,Zhang X Q,Ling Y,Huang T,Huang L K.Constructing SSR fingerprinting of DUS testing materials in Hemarthria.Molecular Plant Breeding,2017,15(5):1790-1799.(in Chinese)
[20]闵学阳,刘文献,张正社,韦兴燚,齐晓,张义,刘志鹏,王彦荣.苜蓿DUS测试标准品种SSR分子标记指纹图谱的构建.草业学报,2017,26(11):47-56.Min X Y,Liu W X,Zhang Z S,Wei X Y,Qi X,Zhang Y,Liu Z P.Wang Y R.Construction of SSR marker fingerprint database of standard alfalfa varieties utilizing DUS tests.Acta Prataculturae Sinica,2017,26(11):47-56.(in Chinese)
[2]李新一.加快我国新草品种保护工作.中国饲料,2016(15):28-31,43.Li X Y.Accelerating the protection of the new grass varieties in China.China Feed,2016(15):28-31,43.(in Chinese)
[3]Baum B R.DNA fingerprinting of cereal cultivars for intellectual property rights protection.UK:In proceedings of the third Symposium on the Taxonomy of Cultivated Plants Edinburgh,1998.
[4]蒋林峰,黄琳凯,张新全,李宁,王鹏喜.鸭茅重要种质DUS测试及农艺性状评价筛选.草地学报,2017,25(2):351-360.Jiang L F,Huang L K,Zhang X Q,Li L,Wang P X.DUS testing and agronomic character evaluation-screening of important Cocksfoot Germplasm.Acta Agrestia Sinica,2017,25(2):351-360.(in Chinese)
[5]卓文飞.我国植物新品种保护及农作物新品种权研究.种业导刊,2015(12):14-19.Zhuo W F.Study on the new plant variety protection and property of the national authorized crop varieties in China.Journal of Seed Industry Guide,2015(12):14-19.(in Chinese)
[6]李亚,刘建秀,向其伯.结缕草属种质资源研究进展.草业学报,2002,11(6):7-14.Li Y,Liu J X,Xiang Q B.Research progress on germplasm resources of Zoysia.Acta Prataculture Sinica,2002,11(6):7-14.(in Chinese)
[7]张新全.草坪草育种学.北京:中国农业出版社,2004.Zhang X Q.Turfgrass Breeding.Beijing:China Agriculture Press,2004.(in Chinese)
[8]中华人民共和国农业部科技发展中心.NY/T 2489-2013,植物新品种特异性,一致性和稳定性测试指南:结缕草属.北京:中国标准出版社,2014.Science and Technology Development Center of the Ministry of Agriculture.NY/T 2489-2013,Guidelines for the Conduct of Tests for Distinctness,Uniformity and Stability:Zoysiagrass.Beijing:Standards Press of China,2014.(in Chinese)
[9]徐振江,刘洪,李春兰,李之林,任永浩.水稻新品种DUS测试数量性状特异性统计分析判别研究.华南农业大学学报,2008,29(1):6-9.Xu Z J,Liu H,Li C L,Li Z L,Ren Y H.Study on the statistical assessment of distinctness of quantitative characteristics in DUS testing of new rice varieties.Journal of South China Agriculture University,2008,29(1):6-9.(in Chinese)
[10]陈海荣,顾晓君.水稻新品种测试中性状差异显著性判定的分析.上海农业学报,2003,19(4):18-20.Chen H R,Gu X J.Analysis on the significance of the difference of traits in new rice varieties.Acta Agriculturae Shanghai,2003,19(4):18-20.(in Chinese)
[11]国家质量技术监督局.BG/T 19557.1-2004,中华人民共和国国家标准:植物新品种特异性、一致性和稳定性测试指南:总则.北京:中国标准出版社,2004.The state bureau of quality and technical supervision.BG/T 19557.1-2004,General Directives for the Conduct of Tests of Distinctness,Uniformity and Stability for New Varieties of Plants.Beijing:Standards Press of China,2004.(in Chinese)
[12]UPOV.Document TGP/8/1(draft1):Use of statistical procedures indistinctness,uniformity and stability testing,[2016-10-28].http://www.upov.int/edocs/tgpdocs/en/tgp_8.pdf
[13]李汝玉,宋国安,杨平平.利用SSR标记进行品种权有争议品种的鉴定.种子,2000(1):66-67.Li R Y,Song G A,Yang P P.Identification of varieties with disputed rights using SSR Markers.Seed,2000(1):66-67.(in Chinese)
[14]张清华.利用多重荧光SSR标记构建甘蓝型油菜品种的DNA指纹图谱.武汉:华中农业大学硕士学位论文,2011.Zhang Q H,Construction of DNA fingerprinting in rapeseed varieties(B.napus L.)using multiplexed fluorescent SSR markers.Master Thesis.Wuhan:Huazhong Agricultural University,2011.(in Chinese)
[15]王立新,常利芳,李宏博,季伟,刘丽华,赵昌平.小麦区试品系DUS测试的分子标记.作物学报,2010,36(7):1114-1125.Wang L X,Chang L F,Li H B,Ji W,Liu L H,Zhao C P.Molecularmarkers for estimating distinctness,uniformity and stability of wheat lines in regional trials.Acta Agrononica Sinica,2010,36(7):1114-1125.(in Chinese)
[16]王艳平,沈奇,张继红,李华勇,吴燕.大麦DUS测试标准品种的遗传多样性分析及指纹图谱的构建.麦类作物学报,2013,33(2):273-278.Wang Y P,Shen Q,Zhang J H,Li H Y,Wu Y.Genetic diversity analysis and building of DNA fingerprints of barley standard varieties in DUS testing based on SSR markers.Journal of Triticeae Crops,2013,33(2):273-278.(in Chinese)
[17]范建光,张海英,宫国义,肖菁,郭绍贵,任毅,张洁,王秀峰,马艳明,许勇.西瓜DUS测试标准品种SSR指纹图谱构建及应用.植物遗传资源学报,2013,14(5):892-899.Fang J G,Zhang H Y,Gong G Y,Xiao J,Guo S G,Ren Y,Zhang J,Wang X F,Ma Y M,Xu Y.Construction and application of SSR fingerprint database of the example varieties in watermelon DUS testing.Journal of Plant Genetic Resources,2013,14(5):892-899.(in Chinese)
[18]丁西朋,张龙,罗小燕,严琳玲,刘国道,白昌军.柱花草DUS测试标准品种DNA指纹图谱构建.草业科学,2015,32(12):2047-2056.Ding X P,Zhang L,Luo X Y,Yan L L,Liu G D,Bai C J.Construction of DNA fingerprinting of Stylosanthes standard cultivars for DUS testing.Pratacultural Science,2015,32(12):2047-2056.(in Chinese)
[19]杨盛婷,武炳超,冯芹,黄秀,张新全,凌瑶,黄婷,黄琳凯.牛鞭草属DUS测试材料的SSR指纹图谱构建.分子植物育种,2017,15(5):1790-1799.Yang S T,Wu B C,Feng Q,Huang X,Zhang X Q,Ling Y,Huang T,Huang L K.Constructing SSR fingerprinting of DUS testing materials in Hemarthria.Molecular Plant Breeding,2017,15(5):1790-1799.(in Chinese)
[20]闵学阳,刘文献,张正社,韦兴燚,齐晓,张义,刘志鹏,王彦荣.苜蓿DUS测试标准品种SSR分子标记指纹图谱的构建.草业学报,2017,26(11):47-56.Min X Y,Liu W X,Zhang Z S,Wei X Y,Qi X,Zhang Y,Liu Z P.Wang Y R.Construction of SSR marker fingerprint database of standard alfalfa varieties utilizing DUS tests.Acta Prataculturae Sinica,2017,26(11):47-56.(in Chinese)