TILLING技术在剑麻中的应用前景
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摘要
定向诱导基因组局部突变(Targeting Induced Local Lesions In Genomes,TILLING)技术自诞生以来在多种模式和非模式植物功能基因组学研究中得到了广泛应用,并获得了大量可用于基础研究的突变体材料和可应用于农作物遗传改良的新种质。这种高通量、低成本的反向遗传学研究方法为剑麻研究带来了新的机遇。目前国内外尚无TILLING 技术应用于剑麻研究的报道,而麻类作物亚麻中则已出现 TILLING 技术成功应用的报道。本文将概述 TILLING 技术的特点,对比其在亚麻中的应用,并结合剑麻 EMS 诱变群体构建策略和剑麻基因组学研究进展,综合探讨该技术在剑麻中的应用前景。
Targeting Induced Local Lesions In Genomes(TILLING) technology has been widely used in functional genomics of various model and non-model plants since its birth, and a large number of mutant materials and new germplasms which could be used in basic research and crop genetic improvement have been obtained. This high-throughput and low-cost reverse genetics research method brings new opportunities for sisal research. Till now,there is no report on the application of TILLING in sisal research around the world, while the successful application of TILLING in flax has been reported. This paper will summarize the characteristics of TILLING, and discuss the potential application of TILLING in sisal according to its application in flax, the strategy of EMS population construction and the research progress of functional genomics in sisal.
Targeting Induced Local Lesions In Genomes(TILLING) technology has been widely used in functional genomics of various model and non-model plants since its birth, and a large number of mutant materials and new germplasms which could be used in basic research and crop genetic improvement have been obtained. This high-throughput and low-cost reverse genetics research method brings new opportunities for sisal research. Till now,there is no report on the application of TILLING in sisal research around the world, while the successful application of TILLING in flax has been reported. This paper will summarize the characteristics of TILLING, and discuss the potential application of TILLING in sisal according to its application in flax, the strategy of EMS population construction and the research progress of functional genomics in sisal.
引文
[1]McCallum CM,Comai L,Greene EA,et al.Targeting induced local lesions in genomes(TILLING)for plant functional genomics[J].Plant Physiology,2000,123(2):439-442
[2]Colbert T,Till BJ,Tompa R,et al.High-throughput screening for induced point mutations[J].Plant Physiology,2001,126(2):480-484
[3]Till BJ,Reynolds SH,Greene EA,et al.Large-scale discovery of induced point mutations with high-throughput TILLING[J].Genome Research,2003,13(3):524-530
[4]McCallum CM,Comai L,Greene EA,et al.Targeted screening for induced mutations[J].Nature Biotechnology,2000,18(4):455-457
[5]Till BJ,Colbert T,Tompa R,et al.High-throughput TILLING for functional genomics[J].Methods in Molecular Biology,2003,236:205-220
[6]Zhou L,Vandersteen J,Wang L,et al.High-resolution DNAmelting curve analysis to establish HLA genotypic identity[J].Tissue Antigens,2004,64(2):156-164
[7]Le Signor C,Savois V,Aubert G,et al.Optimizing TILLINGpopulations for reverse genetics in Medicago truncatula[J].Plant Biotechnology Journal,2009,7(5):430-441
[8]Missirian V,Comai L,Filkov V.Statistical mutation calling from sequenced overlapping DNA pools in TILLING experiments[J].BMC Bioinformatics,2011,12:287
[9]Tsai H,Howell T,Nitcher R,et al.Discovery of rare mutations in populations:TILLING by sequencing[J].Plant Physiology,2011,156(3):1257-1268
[10]Gonzalez M,Xu M,Esteras C,et al.Towards a TILLINGplatform for functional genomics in Piel de Sapo melons[J].BMC Research Notes,2011,4:289
[11]Knoll JE,Ramos ML,Zeng YJ,et al.TILLING for allergen reduction and improvement of quality traits in peanut(Arachis hypogaea L.)[J].BMC Plant Biology,2011,11:81
[12]Sabetta W,Alba V,Blanco A,et al.sunTILL:a TILLINGresource for gene function analysis in sunflower[J].Plant Methods,2011,7(1):20
[13]Blomstedt CK,Gleadow RM,O'Donnell N,et al.A combined biochemical screen and TILLING approach identifies mutations in Sorghum bicolor L.Moench resulting in acyanogenic forage production[J].Plant Biotechnology Journal,2012,10(1):54-66
[14]Chantreau M,Grec S,Gutierrez L,et al.PT-Flax(phenotyping and TILLinG of flax):development of a flax(Linum usitatissimum L.)mutant population and TILLING platform for forward and reverse genetics[J].BMC Plant Biology,2013,13:159
[15]Vicente-Dolera N,Troadec C,Moya M,et al.First TILLINGplatform in Cucurbita pepo:a new mutant resource for gene function and crop improvement[J].Plos One,2014,9(11):e112743
[16]龚友才,粟建光.麻类作物诱变育种的现状与进展[J].中国麻业,2002,24(4):14-17
[17]Kurowska M,Daszkowska-Golec A,Gruszka D,et al.TILLING:a shortcut in functional genomics[J].Journal of Applied Genetics,2011,52(4):371-390
[18]Till BJ,Jankowicz-Cieslak J,Sagi L,et al.Discovery of nucleotide polymorphisms in the Musa gene pool by Ecotilling[J].Theoretical and Applied Genetics,2010,121(7):1381-1389
[19]Jain S,Till B,Suprasanna P,Roux N.Mutations and cultivar development of banana.In Banana Breeding,Progress and Challenges[B].Pillay M,Tenkouano A.Eds.CRC Press:Boca Raton,FL,USA,2011:pp.201-215
[20]Zhou W,Zhang Y,Lu J,et al.Construction and evaluation of normalized cDNA libraries enriched with full-length sequences for rapid discovery of new genes from Sisal(Agave sisalana Perr.)different developmental stages[J].International Journal of Molecular Science,2012,13(10):13150-13168
[21]Huang X,Xiao M,Xi J,et al.De novo transcriptome assembly of Agave H11648 by illumina sequencing and identification of cellulose synthase genes in Agave species[J].Genes,2019,10(2):103
[22]Sarwar MB,Ahmad Z,Rashid B,et al.De novo assembly of Agave sisalana transcriptome in response to drought stress provides insight into the tolerance mechanisms[J].Scientific Reports,2019,9(1):396
[23]Cortes-Romero C,Martinez-Hernandez A,MelladoMojica E,et al.Molecular and functional characterization of novel fructosyltransferases and invertases from Agave tequilana[J].Plos One,2012,7(4):e35878
[24]Suarez-Gonzalez EM,Lopez MG,Delano-Frier JP,et al.Expression of the 1-SST and 1-FFT genes and consequent fructan accumulation in Agave tequilana and A.inaequidens is differentially induced by diverse(a)biotic-stress related elicitors[J].Journal of Plant Physiology,2014,171(3-4):359-372
[25]Corbin KR,Byrt CS,Bauer S,et al.Prospecting for energyrich renewable raw materials:Agave leaf case study[J].Plos One,2015,10(8):e0135382
[26]Cervantes-Pérez SA,Espinal-Centeno A,Oropeza-Aburto A,et al.Transcriptional profiling of the CAM plant Agave salmiana reveals conservation of a genetic program for regeneration[J].Developmental Biology,2018,442(1):28-39
[27]Abraham PE,Yin H,Borland AM,et al.Transcript,protein and metabolite temporal dynamics in the CAM plant Agave[J].Nature Plants,2016,2:16178
[28]Yin H,Guo HB,Weston DJ,et al.Diel rewiring and positive selection of ancient plant proteins enabled evolution of CAMphotosynthesis in Agave[J].BMC Genomics,2018,19:588
[29]Huang X,Wang B,Xi J,et al.Transcriptome comparison reveals distinct selection patterns in domesticated and wild Agave species,the important CAM plants[J].International Journal of Genomics,2018:5716518
[2]Colbert T,Till BJ,Tompa R,et al.High-throughput screening for induced point mutations[J].Plant Physiology,2001,126(2):480-484
[3]Till BJ,Reynolds SH,Greene EA,et al.Large-scale discovery of induced point mutations with high-throughput TILLING[J].Genome Research,2003,13(3):524-530
[4]McCallum CM,Comai L,Greene EA,et al.Targeted screening for induced mutations[J].Nature Biotechnology,2000,18(4):455-457
[5]Till BJ,Colbert T,Tompa R,et al.High-throughput TILLING for functional genomics[J].Methods in Molecular Biology,2003,236:205-220
[6]Zhou L,Vandersteen J,Wang L,et al.High-resolution DNAmelting curve analysis to establish HLA genotypic identity[J].Tissue Antigens,2004,64(2):156-164
[7]Le Signor C,Savois V,Aubert G,et al.Optimizing TILLINGpopulations for reverse genetics in Medicago truncatula[J].Plant Biotechnology Journal,2009,7(5):430-441
[8]Missirian V,Comai L,Filkov V.Statistical mutation calling from sequenced overlapping DNA pools in TILLING experiments[J].BMC Bioinformatics,2011,12:287
[9]Tsai H,Howell T,Nitcher R,et al.Discovery of rare mutations in populations:TILLING by sequencing[J].Plant Physiology,2011,156(3):1257-1268
[10]Gonzalez M,Xu M,Esteras C,et al.Towards a TILLINGplatform for functional genomics in Piel de Sapo melons[J].BMC Research Notes,2011,4:289
[11]Knoll JE,Ramos ML,Zeng YJ,et al.TILLING for allergen reduction and improvement of quality traits in peanut(Arachis hypogaea L.)[J].BMC Plant Biology,2011,11:81
[12]Sabetta W,Alba V,Blanco A,et al.sunTILL:a TILLINGresource for gene function analysis in sunflower[J].Plant Methods,2011,7(1):20
[13]Blomstedt CK,Gleadow RM,O'Donnell N,et al.A combined biochemical screen and TILLING approach identifies mutations in Sorghum bicolor L.Moench resulting in acyanogenic forage production[J].Plant Biotechnology Journal,2012,10(1):54-66
[14]Chantreau M,Grec S,Gutierrez L,et al.PT-Flax(phenotyping and TILLinG of flax):development of a flax(Linum usitatissimum L.)mutant population and TILLING platform for forward and reverse genetics[J].BMC Plant Biology,2013,13:159
[15]Vicente-Dolera N,Troadec C,Moya M,et al.First TILLINGplatform in Cucurbita pepo:a new mutant resource for gene function and crop improvement[J].Plos One,2014,9(11):e112743
[16]龚友才,粟建光.麻类作物诱变育种的现状与进展[J].中国麻业,2002,24(4):14-17
[17]Kurowska M,Daszkowska-Golec A,Gruszka D,et al.TILLING:a shortcut in functional genomics[J].Journal of Applied Genetics,2011,52(4):371-390
[18]Till BJ,Jankowicz-Cieslak J,Sagi L,et al.Discovery of nucleotide polymorphisms in the Musa gene pool by Ecotilling[J].Theoretical and Applied Genetics,2010,121(7):1381-1389
[19]Jain S,Till B,Suprasanna P,Roux N.Mutations and cultivar development of banana.In Banana Breeding,Progress and Challenges[B].Pillay M,Tenkouano A.Eds.CRC Press:Boca Raton,FL,USA,2011:pp.201-215
[20]Zhou W,Zhang Y,Lu J,et al.Construction and evaluation of normalized cDNA libraries enriched with full-length sequences for rapid discovery of new genes from Sisal(Agave sisalana Perr.)different developmental stages[J].International Journal of Molecular Science,2012,13(10):13150-13168
[21]Huang X,Xiao M,Xi J,et al.De novo transcriptome assembly of Agave H11648 by illumina sequencing and identification of cellulose synthase genes in Agave species[J].Genes,2019,10(2):103
[22]Sarwar MB,Ahmad Z,Rashid B,et al.De novo assembly of Agave sisalana transcriptome in response to drought stress provides insight into the tolerance mechanisms[J].Scientific Reports,2019,9(1):396
[23]Cortes-Romero C,Martinez-Hernandez A,MelladoMojica E,et al.Molecular and functional characterization of novel fructosyltransferases and invertases from Agave tequilana[J].Plos One,2012,7(4):e35878
[24]Suarez-Gonzalez EM,Lopez MG,Delano-Frier JP,et al.Expression of the 1-SST and 1-FFT genes and consequent fructan accumulation in Agave tequilana and A.inaequidens is differentially induced by diverse(a)biotic-stress related elicitors[J].Journal of Plant Physiology,2014,171(3-4):359-372
[25]Corbin KR,Byrt CS,Bauer S,et al.Prospecting for energyrich renewable raw materials:Agave leaf case study[J].Plos One,2015,10(8):e0135382
[26]Cervantes-Pérez SA,Espinal-Centeno A,Oropeza-Aburto A,et al.Transcriptional profiling of the CAM plant Agave salmiana reveals conservation of a genetic program for regeneration[J].Developmental Biology,2018,442(1):28-39
[27]Abraham PE,Yin H,Borland AM,et al.Transcript,protein and metabolite temporal dynamics in the CAM plant Agave[J].Nature Plants,2016,2:16178
[28]Yin H,Guo HB,Weston DJ,et al.Diel rewiring and positive selection of ancient plant proteins enabled evolution of CAMphotosynthesis in Agave[J].BMC Genomics,2018,19:588
[29]Huang X,Wang B,Xi J,et al.Transcriptome comparison reveals distinct selection patterns in domesticated and wild Agave species,the important CAM plants[J].International Journal of Genomics,2018:5716518