紫外杀菌在洁净工作台中应用效果研究
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摘要
紫外灯是用于实验室洁净工作台中的重要杀菌装置,为了研究该设备操作空间环境紫外杀菌效果及维持无菌化时间,本文以细菌分布密度为4.08×102CFU·cm-2的含菌培养基作为试验材料,通过研究发现,当杀菌时间达到30 min后可保证细菌全部杀灭,而且采用75%乙醇辅助紫外杀菌比单纯紫外杀菌的效果更加显著,经杀菌后的洁净工作台空间可维持无菌环境达12 h.表明紫外杀菌是保障操作空间无菌的有效方式,为正确把握紫外灭菌时间及合理使用洁净工作台提供了重要参考.
The ultraviolet lamp is a critical sterilizer used in the laboratory clean bench. It's sterilizing effect in operation space and maintenance of sterilization were studied. The bacterial culture media with a distribution density of 4.08×102 CFU·cm-2 were used. The bacteria were all sterilized using ultraviolet sterilization for 30 min,and sterilization effect was more significant by using ultraviolet supplemented with 75% ethanol than simple ultraviolet light. Also,space in clean bench could be maintained sterile for 12 h after UV treatment. Therefore,we demonstrate that ultraviolet sterilization is an effective way to ensure sterile space,and provide an important reference on correctly grasping the sterilization time.
The ultraviolet lamp is a critical sterilizer used in the laboratory clean bench. It's sterilizing effect in operation space and maintenance of sterilization were studied. The bacterial culture media with a distribution density of 4.08×102 CFU·cm-2 were used. The bacteria were all sterilized using ultraviolet sterilization for 30 min,and sterilization effect was more significant by using ultraviolet supplemented with 75% ethanol than simple ultraviolet light. Also,space in clean bench could be maintained sterile for 12 h after UV treatment. Therefore,we demonstrate that ultraviolet sterilization is an effective way to ensure sterile space,and provide an important reference on correctly grasping the sterilization time.
引文
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[11]刘钟栋,陈肇锬,欧军辉,等.微波紫外线协同生物学作用及紫外线微波炉杀菌研究[J].食品科技,2007(12):140-142.
[12]龚媛,刘敦华,曲云卿,等.响应面法优化臭氧与紫外复合处理枸杞干果杀菌工艺[J].食品工业科技,2015,36(12):254-258.
[13]张福云,况凡.紫外线灯杀菌效果影响因素试验观察[J].中国消毒学杂志,2005,22(3):318-319.
[14]杨明扬,王飞,孟庆阳.紫外线降低片猪肉表面微生物研究[J].肉类工业,2018(4):27-29.
[2]Barstow C K,Dotson A D,Linden K G.Assessing point-of-use ultraviolet disinfection for safe water in urban developing communities[J].Journal of Water&Health,2014,12(4):663-669.
[3]Wedum A G,Jr H E,Phillips G B.Ultraviolet sterilization in microbiological laboratories[J].Public Health Reports,1956,71(4):331-336.
[4]孔繁贞,冯建萍,魏绍振,等.紫外线对微生物消毒效果的影响因素及质控措施[J].青海畜牧兽医杂志,2017,47(2):64-66.
[5]Knox R W,Demons S T,Cunningham C W.A Novel Method to Decontaminate Surgical Instruments for Operational and Austere Environments[J].Wilderness&Environmental Medicine,2015,26(4):509-513.
[6]薛冰.生物安全柜紫外辐射防护措施探析[J].安全,2017,38(12):33-35.
[7]宋晓雪,胡文忠,毕阳,等.鲜切果蔬中致腐微生物污染及其非热杀菌的研究进展[J].食品工业科技,2014,35(10):351-354.
[8]李影球,周光宏,徐幸莲.四种非热杀菌技术在肉类中的应用[J].食品工业科技,2013,34(17):354-359.
[9]荣芳,窦锐,毛月琴,等.20W紫外线消毒灯临床使用情况监测[J].中国消毒学杂志,2015,32(4):408-409.
[10]Cantwell R E,Hofmann R,Templeton M R. Interactions between humic matter and bacteria when disinfecting water with UV light[J].Journal of applied microbiology,2008,105(1):25-35.
[11]刘钟栋,陈肇锬,欧军辉,等.微波紫外线协同生物学作用及紫外线微波炉杀菌研究[J].食品科技,2007(12):140-142.
[12]龚媛,刘敦华,曲云卿,等.响应面法优化臭氧与紫外复合处理枸杞干果杀菌工艺[J].食品工业科技,2015,36(12):254-258.
[13]张福云,况凡.紫外线灯杀菌效果影响因素试验观察[J].中国消毒学杂志,2005,22(3):318-319.
[14]杨明扬,王飞,孟庆阳.紫外线降低片猪肉表面微生物研究[J].肉类工业,2018(4):27-29.