乳腺癌组织中Livin和Smac的表达及其临床意义
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摘要
目的:探讨Livin和Smac蛋白在乳腺癌组织和癌旁组织中的表达及其临床意义。方法:收集乳腺癌患者43例,采用免疫组织化学S-P法检测Livin及Smac在乳腺癌组织、癌旁组织中的表达情况,应用χ~2检验、K-M法分析临床特征及生存情况。结果:Livin在乳腺癌组织中的阳性表达率为79.1%,癌旁组织中表达率为48.8%,差异有统计学意义(P<0.05);Smac在乳腺癌组织中的阳性表达率为74.4%,癌旁组织中表达率为93.0%,差异也有统计学意义(P<0.05)。Livin和Smac蛋白的表达均与乳腺癌的TNM分期及淋巴结转移情况相关,其差异有统计学意义(两组P<0.05),并且乳腺癌组织中Livin与Smac的表达情况呈负相关(P<0.05)。此外,Livin阴性组与阳性组中5年无病生存率分别为66.7%和23.5%,Livin阴性组比阳性组无病生存期有所延长(P<0.05),而Smac阴性组与阳性组的无病生存期差异无统计学意义(P>0.05)。结论:Livin、Smac在乳腺癌中的表达水平呈负相关,提示可能有某种机制在调节这两者的表达。另外,Livin阴性组患者的5年无病生存期有所延长,提示该蛋白或可作为乳腺癌患者的预后指标,值得进一步研究。
Objective: To explore the expression and clinic-pathological characteristics of Livin and Smac in breast carcinoma tissue. Methods: S-P immunohistochemical staining was adopted to examine the expression of Livin and Smac; breast cancer samples(n=43) were assembled from West China Hospital, Sichuan University. Results: The positive expression of Livin in breast cancer tissue was 79.1%, and it was 48.8% in paracarcinoma tissue. The difference between the two groups was statistically significant(P<0.05). Meanwhile, the positive expression of Smac in breast cancer tissue was 74.4%, and it was 93.0% in paracarcinoma tissue. The difference between the two groups was statistically significant(P<0.05). Livin protein expression in breast cancer was closely related to clinical stage and lymph node metastasis(P<0.05). A negative correlation was found between the expression of Livin and that of Samc in breast cancer(P<0.05). The 5-year disease free survival(DFS) of the Livin-negative group(66.7%) was significantly longer than that of the Livin-positive group(23.5%, P<0.05), while the 5-year DFS of the Smac-negative group and that of the Smac-positive group were not significantly different(P>0.05). Conclusion: The expression level of Livin in breast cancer tissue is negatively related to the expression level of Smac in breast cancer tissue, indicating a negative correlation between these two proteins. Meanwhile, the 5 year DFS of Livin-negative patients is significantly longer than that of the Livin-positive patients. Therefore, Livin may be employed as a prognosis indicator for breast cancer patients.
Objective: To explore the expression and clinic-pathological characteristics of Livin and Smac in breast carcinoma tissue. Methods: S-P immunohistochemical staining was adopted to examine the expression of Livin and Smac; breast cancer samples(n=43) were assembled from West China Hospital, Sichuan University. Results: The positive expression of Livin in breast cancer tissue was 79.1%, and it was 48.8% in paracarcinoma tissue. The difference between the two groups was statistically significant(P<0.05). Meanwhile, the positive expression of Smac in breast cancer tissue was 74.4%, and it was 93.0% in paracarcinoma tissue. The difference between the two groups was statistically significant(P<0.05). Livin protein expression in breast cancer was closely related to clinical stage and lymph node metastasis(P<0.05). A negative correlation was found between the expression of Livin and that of Samc in breast cancer(P<0.05). The 5-year disease free survival(DFS) of the Livin-negative group(66.7%) was significantly longer than that of the Livin-positive group(23.5%, P<0.05), while the 5-year DFS of the Smac-negative group and that of the Smac-positive group were not significantly different(P>0.05). Conclusion: The expression level of Livin in breast cancer tissue is negatively related to the expression level of Smac in breast cancer tissue, indicating a negative correlation between these two proteins. Meanwhile, the 5 year DFS of Livin-negative patients is significantly longer than that of the Livin-positive patients. Therefore, Livin may be employed as a prognosis indicator for breast cancer patients.
引文
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[17] Du C,Fang M,Li Y,et al.Smac,a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition[J].Cell,2000,102(1)∶33- 42.
[18] Kong DQ,Liu Y,Li L,et al.Downregulation of Smac attenuates H2O2-induced apoptosis via endoplasmic reticulum stress in human lens epithelial cells [J].Medicine (Baltimore),2017,96(27)∶e7419.
[19] Bai L,Wang S.Targeting apoptosis pathways for new cancer therapeutics[J].Annu Rev Med,2014,65 ∶139-155.
[2] 魏洪亮,肖晶晶,凌瑞.男性乳腺癌发病特征及内分泌治疗[J].肿瘤预防与治疗,2018,31(3)∶227-231.
[3] LaCasse EC,Baird S,Korneluk RG,et al.The inhibitors of apoptosis(IAPs) and their emerging role in cancer[J].Oncogene,1998,17(25)∶3247-3259.
[4] Kashkar H,Haefs C,Shin H,et al.XIAP-mediated Caspase inhibition in Hodgkin’s lymphomaderived B cells[J].J Exp Med,2003,198(2)∶341-347.
[5] 许良中,杨文涛.免疫组织化学反应结果的判定标准[J].中国癌症杂志.1996,6(4)∶229-231.
[6] Jaattela M.Escaping cell death:survival proteins in cancer[J].Exp Cell Res,1999,248(1)∶30- 43.
[7] Wang J,Zhang X,Wei P,et al.Livin,Survivin and Caspase 3 as early recurrence markers in non-muscle-invasive bladder cancer[J].World J Urol,2014,32(6)∶1477-1484.
[8] Su Q,Wang L,Wei G,et al.Livin serves as a prognostic marker for mid-distal rectal cancer and a target of mid-distal rectal cancer treatment [J].Oncol Lett,2017,14(6)∶7759-7766.
[9] Dubrez-Daloz L,Dupoux A,Cartier J,et al.IAPs:more than just inhibitors of apoptosis proteins[J].Cell Cycle,2008,7(8)∶1036-1046.
[10] Chen L,Ren GS,Li F,et al.Expression of livin and vascular endothelial growth factor in different clinical stages of human esophageal carcinoma [J].World J Gastroenterol,2008,14(37)∶5749-5754.
[11] Augello C,Caruso L,Maggioni M,et al.Inhibitors of apoptosis proteins (IAPs) expression and their prognostic signifcance in hepatocellular carcinoma[J].BMC Cancer,2009,9(125)∶1186
[12] Yang YL,Lin SR,Chen JS,et al.Expression and prognostic signifcance of the apoptotic genes BCL2L13,Livin,and CASP8AP2 in childhood acute lymphoblastic leukemia[J].Leuk Res,2010,34(1)∶18-23.
[13] Ye L,Song X,Li S,et al.Livin-a promotes cell proliferation by regulating G1-S cell cycle transition in prostate cancer[J].Prostate,2011,71(1)∶42-51.
[14] Cheng T,Zhang JG,Cheng YH,et al.Relationship between PTEN and Livin expression and malignancy of renal cell carcinomas[J].Asian Pac J Cancer Prev,2012,13(6)∶2681-2685.
[15] Ibrahim L,Aladle D,Mansour A,et al.Expression and prognostic signifcance of livin/BIRC7 in childhood acute lymphoblastic leukemia[J].Med Oncol,2014,31(5)∶941.
[16] 赵秋民.食管鳞癌组织中Livin和Smac表达的意义[J].肿瘤防治研究,2009,36(2)∶110-114.
[17] Du C,Fang M,Li Y,et al.Smac,a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition[J].Cell,2000,102(1)∶33- 42.
[18] Kong DQ,Liu Y,Li L,et al.Downregulation of Smac attenuates H2O2-induced apoptosis via endoplasmic reticulum stress in human lens epithelial cells [J].Medicine (Baltimore),2017,96(27)∶e7419.
[19] Bai L,Wang S.Targeting apoptosis pathways for new cancer therapeutics[J].Annu Rev Med,2014,65 ∶139-155.