佛手标准汤剂中两种成分的测定方法研究
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摘要
为提高佛手配方颗粒的质量评价标准,利用佛手标准汤剂控制佛手配方颗粒的内在质量。以15批不同产地的佛手饮片为原料,制备佛手标准汤剂,建立含量测定方法,以柚皮苷、橙皮苷为指标成分,测定了15批佛手标准汤剂的相关含量。结果表明:柚皮苷、橙皮苷分别在0.025 1～0.125 5,0.160 5～0.802 3μg范围内线性关系良好(r=0.999 9),平均加样回收率分别为102.27%和101.48%,重复性考察RSD值分别为0.78%和0.89%,12 h稳定性考察RSD值分别为0.59%和1.46%。所提出的方法能同时测定柚皮苷和橙波苷两种成分,节约了分析时间,提高了佛手产品的质量标准。
In order to improve the quality evaluation standard of Citri sarcodactylis fuctus formula granules, the internal quality of Citri sarcodactylis fuctus granules is controlled by standard decoction of Citri sarcodactylis fuctus. Sandard decoctions of Citri sarcodactylis fuctus is prepared with 15 batches of Citri sarcodactylis fuctus pieces from different producing areas as raw material, the content measuring method is established, and with the contents of naringin and hesperidin as indicators, the contents of the 15 batches of standard decoction of Citri sarcodactylis fuctus are determined. The results show that the naringin and hesperidin are in linear in the range of 0.025 1～0.125 5 μg and 0.160 5～0.802 3 μg, respectively(r=0.999 9), and the average recovery rates are 102.27% and 101.48%, respectively. The reproducibility of RSD is 0.78%, 0.89%, and 12-hour stability RSD values are 0.59% and 1.46%. The method can simultaneously determine naringin and hesperidin, thus saving analysis time and improving the quality standard of Citri sarcodactylis fuctus products.
In order to improve the quality evaluation standard of Citri sarcodactylis fuctus formula granules, the internal quality of Citri sarcodactylis fuctus granules is controlled by standard decoction of Citri sarcodactylis fuctus. Sandard decoctions of Citri sarcodactylis fuctus is prepared with 15 batches of Citri sarcodactylis fuctus pieces from different producing areas as raw material, the content measuring method is established, and with the contents of naringin and hesperidin as indicators, the contents of the 15 batches of standard decoction of Citri sarcodactylis fuctus are determined. The results show that the naringin and hesperidin are in linear in the range of 0.025 1～0.125 5 μg and 0.160 5～0.802 3 μg, respectively(r=0.999 9), and the average recovery rates are 102.27% and 101.48%, respectively. The reproducibility of RSD is 0.78%, 0.89%, and 12-hour stability RSD values are 0.59% and 1.46%. The method can simultaneously determine naringin and hesperidin, thus saving analysis time and improving the quality standard of Citri sarcodactylis fuctus products.
引文
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[11] 曹斯琼,陈向东,黄丹丹,等.佛手配方颗粒UPLC指纹图谱研究[J].海峡药学,2018,30(6):48-52.CAO Siqiong,CHEN Xiangdong,HUANG Dandan,et al.UPLC fingerprint analysis of Citrus medica L.var.sarcodactylis Swingle formula granules[J].Strait Pharmaceutical Journal,2018,30(6):48-52.
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[13] 林乐维,蒋林,郝大庆,等.不同产地广佛手药材HPLC指纹图谱的研究[J].中成药,2009,31(12):1805-1808.LIN Lewei,JIANG Lin,HAO Daqing,et al.HPLC fingerprint of fructus Citri sarcodactylis from Guangdong province[J].Chinses Traditional Patent Medicine,2009,31(12):1805-1808.
[14] 孙福仁,李军山,马浩,等.基于UPLC特征图谱与主成分分析评价白芷配方颗粒质量研究[J].河北工业科技,2018,35(6):454-458.SUN Furen,LI Junshan,MA Hao,et al.Study on evaluation of the quality of formula granule of Angelicae dahuricae based on UPLC and PCA[J].Hebei Journal of Industrial Science and Technology,2018,35(6):454-458.
[15] 田方,李军山,常云凤,等.一测多评法同时测定车前子配方颗粒中3种成分含量[J].河北工业科技,2018,35(6):459-464.TIAN Fang,LI Junshan,CHANG Yunfeng,et al.Simultaneous determination of three constituents in Plantago seed formula granules by QAMS[J].Hebei Journal of Industrial Science and Technology,2018,35(6):459-464.
[2] 张颖,孔令义.佛手化学成分的研究[J].中国现代中药,2008,8(6):16-17.ZHANG Ying,KONG Lingyi.Studies on the constituents of Citrus medica L.var.sarcodactylis Swingle[J].Modern Chinese Medicine,2008,8(6):16-17.
[3] 高幼衡,黄海波,徐鸿华,等.佛手化学成分研究[J].中药材,2002,25(9):639-640.GAO Youheng,HUANG Haibo,XU Honghua,et al.Studies on the chemical consitutents of Citrus medica L.var.sarcodactylis[J].Journal of Chinese Medicinal Materials,2002,25(9):639-640.
[4] 史晶晶,时博.橙皮苷对高脂血症模型大鼠代谢紊乱的影响[J].中医学报,2016,31(4):554-557.SHI Jingjing,SHI Bo.Effect of hesperidin on lipid metabolism disorder in hyperlipemia model rats[J].Acta Chinese Medicine,2016,31(4):554-557.
[5] PARAFATI M,LUSCIA A,MORITTA V M,et al.Bergamed polyphenol fraction prevents nonalcoholic farty liver disease via stimulation of lipophagy in cafeteria dietinduced rat model of metabolic syndrome[J].Journal of Nutritional Biochemistry,2015,26(9):938-948.
[6] 吴昊,橘波,何俏军,等.新橙皮苷在制备预防糖尿病药物中的应用[P].中国专利:CN102772424A,2012-06-13.
[7] 金晓玲,徐丽珊,何新霞.佛手醇提取液的药理作用研究[J].中国中药杂志,2002,27(8):604-606.
[8] 宋剑锋,富同义.RP-HPLC法测定佛手饮片中橙皮苷含量[J].中华中医药学刊,2007,25(5):1036-1037.SONG Jianfeng,FU Tongyi.Content determination of hesperidin in fructus Citris sarcodactylis by RP-HPLC[J].Chinese Archives of Traditional Chinese Medicine,2007,25(5):1036-1037.
[9] 曾建伟,吴锦忠,林忠宁,等.HPLC测定不同产地佛手中橙皮苷的含量[J].中国实验方剂学杂志,2012,18(19):97-99.ZENG Jianwei,WU Jinzhong,LIN Zhongning,et al.Determination of hesperidin in fructus Citris sarcodactylis from different provenances by HPLC[J].Chinese Journal of Experimental Traditional Medical Formalae,2012,18(19):97-99.
[10] 金晓玲,曹诣斌,徐丽珊,等.佛手氨基酸及多糖含量的测定[J].浙江师范大学学报(自然科学版),2002,25(1):66-68.JIN Xiaoling,CAO Yibin,XU Lishan,et al.Contents of amino acids and polysaccharides from Bergamot[J].Journal of Zhejiang Normal University(Natural Sciences),2002,25(1):66-68.
[11] 曹斯琼,陈向东,黄丹丹,等.佛手配方颗粒UPLC指纹图谱研究[J].海峡药学,2018,30(6):48-52.CAO Siqiong,CHEN Xiangdong,HUANG Dandan,et al.UPLC fingerprint analysis of Citrus medica L.var.sarcodactylis Swingle formula granules[J].Strait Pharmaceutical Journal,2018,30(6):48-52.
[12] 李金玉,贺文婷,王喜英.不同采收期广佛手指纹图谱研究[J].安徽农业科学,2015,43(14):99-101.LI Jinyu,HE Wenting,WANG Xiying.HPLC fingerprint of fructus Citri sarcodactylis in different picking times[J].Journal of Anhui Agricultural Sciences,2015,43(14):99-101.
[13] 林乐维,蒋林,郝大庆,等.不同产地广佛手药材HPLC指纹图谱的研究[J].中成药,2009,31(12):1805-1808.LIN Lewei,JIANG Lin,HAO Daqing,et al.HPLC fingerprint of fructus Citri sarcodactylis from Guangdong province[J].Chinses Traditional Patent Medicine,2009,31(12):1805-1808.
[14] 孙福仁,李军山,马浩,等.基于UPLC特征图谱与主成分分析评价白芷配方颗粒质量研究[J].河北工业科技,2018,35(6):454-458.SUN Furen,LI Junshan,MA Hao,et al.Study on evaluation of the quality of formula granule of Angelicae dahuricae based on UPLC and PCA[J].Hebei Journal of Industrial Science and Technology,2018,35(6):454-458.
[15] 田方,李军山,常云凤,等.一测多评法同时测定车前子配方颗粒中3种成分含量[J].河北工业科技,2018,35(6):459-464.TIAN Fang,LI Junshan,CHANG Yunfeng,et al.Simultaneous determination of three constituents in Plantago seed formula granules by QAMS[J].Hebei Journal of Industrial Science and Technology,2018,35(6):459-464.
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