新麦草种质的SSR遗传多样性及群体结构分析
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摘要
利用38对SSR引物对来源于不同国家与地区的171份野生和栽培新麦草单株种质进行遗传多样性和群体结构分析。研究结果显示,有效引物在新麦草单株材料间共获得308个位点,多态性位点275个,多态性百分率为89.29%,供试新麦草材料的遗传相似性系数介于0.537～0.899之间,平均为0.742。群体结构分析、主成分分析与NJ聚类法均将供试材料分为3个类群,且类群间的材料相互渗透形成混合类群,分类结果与遗传相似性系数分析表明171份新麦草单株种质分布较集中;3种分类方法对新麦草材料3个不同类群的划分结果基本一致,但混合类群的划分存在差异,每个类群的新麦草单株材料来源背景不同,未表现出明确的地域性规律。
Genetic diversity and population structure analysis of 171 wild and cultivated PsathyrostachysNevski individual germplasms from different countries and regions were conducted by 38 pairs of SSR primers.The result by using the primers revealed a total of 308 loci among Psathyrostachys Nevski individuals,with 275 polymorphic loci and a polymorphism percentage of 89.29%.The genetic similarity coefficient of the tested materials ranged from 0.537 to 0.899 with an average of 0.742.These accessions were divided into three groups by population structure analysis,principal component analysis and NJ clustering.The genetic similarity coefficient analysis showed that the distribution of Psathyrostachys Nevski individuals was more concentrated.The classification using three methods generally revealed three different groups of Psathyrostachys Nevski materials,despite that there were slightly different in the divisions of mixed groups.Since these Psathyrostachys Nevski individuals in each group showed different on genetic background,no clear correlation between geographic distribution and genetic diversity was observed.
Genetic diversity and population structure analysis of 171 wild and cultivated PsathyrostachysNevski individual germplasms from different countries and regions were conducted by 38 pairs of SSR primers.The result by using the primers revealed a total of 308 loci among Psathyrostachys Nevski individuals,with 275 polymorphic loci and a polymorphism percentage of 89.29%.The genetic similarity coefficient of the tested materials ranged from 0.537 to 0.899 with an average of 0.742.These accessions were divided into three groups by population structure analysis,principal component analysis and NJ clustering.The genetic similarity coefficient analysis showed that the distribution of Psathyrostachys Nevski individuals was more concentrated.The classification using three methods generally revealed three different groups of Psathyrostachys Nevski materials,despite that there were slightly different in the divisions of mixed groups.Since these Psathyrostachys Nevski individuals in each group showed different on genetic background,no clear correlation between geographic distribution and genetic diversity was observed.
引文
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[20]宿俊吉,柴守诚,刘伟华,杨欣明,李立会.普通小麦SSR和EST-SSR引物对冰草通用性的比较分析.西北植物学报,2007,27(7):1311-1316SuJJ,ChaiSC,Liu WH,YangXM,LiLH.Comparison oftransferabilityofcommonwheatSSRsandEST-SSRsto AgropyroncristatumL.ActaBotanicaBoreali-Occidentalia Sinica,2007,27(7):1311-1316
[21]邹玉婷.小麦EST-SSR标记对小麦及其近缘种属的遗传多样性研究.成都:四川农业大学,2010Zou Y T.Genetic diversity among triticeaes species as revealed bywheatEST-SSRmarker.Chengdu:Sichuan Agriculture University,2010
[22]张成林,郭志慧,张新全,白史且,张昌兵,李平,马啸.利用SSR标记对垂穗披碱草和老芒麦进行物种鉴定和遗传变异分析.植物遗传资源学报,2016,17(3):416-422ZhangCL,GuoZH,ZhangXQ,BaiSQ,ZhangCB,LiP,MaX.Ananalysisofspeciesidentificationandgenetic divergence of drooping wildrye(Elymus nutans)and siberian wildrye(E.sibiricus)by SSR markers. Journal of Plant Genetic Resources,2016,17(3):416-422
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[24]章秋平,刘威生,刘硕,魏潇,刘宁.杏遗传多样性评价所需SSR等位变异数目估计.果树学报,2015,32(2):186-191ZhangQP,Liu WS,LiuS,WeiX,LiuN.Anestimationof SSR alleles numbers for analyzing genetic diversity of apricot.Journal of Fruit Science,2015,32(2):186-191
[25]张晨,云岚,白亚利,程鹏飞,赵彦.新麦草种质资源的SSR遗传多样性分析.中国草地学报,2017,39(5):24-30Zhang C,Yun L,Bai Y L,Cheng P F,Zhao Y.Genetic diversity analysisof28Psathyrostachysjunceagermplasmresources based on SSR molecular markers.Chinese Journal of Grassland,2017,39(5):24-30
[26]杭焱,金燕,卢宝荣.濒危植物华山新麦草(Psathyrostachys huashanica)的遗传多样性及其保护.复旦学报:自然科学版,2004,43(2):260-266Hang Y,Jin Y,LuBR.Geneticdiversityoftheendangered species PsathyrostachyshuashanicainChinaanditsstrategic conservation.JournalofFudanUniversity:NaturalScience Edition,2004,43(2):260-266
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[2]白可喻,王培,韩建国.放牧强度对新麦草人工草地氮素在牧草与土壤中的分配和动态的影响.草地学报,1999,7(1):46-53Bai K Y,Wang P,Han J G.Nitrogen distribution at grass—soil system and growing dynamic on Russian Wildryegrass pasture as influenced by grazing. Acta Agrestia Sinica,1999,7(1):46-53
[3]王勇,徐春波,石凤翎,云锦凤.蒙农4号新麦草种子产量影响因子研究.种子,2014,33(9):87-89Wang Y,XuCB,ShiFL,YunJF.Studiesonseedyields influence factors of Psathyrostachys Juncea cv.Mengnong No.4.Seed,2014,33(9):87-89
[4]谷安琳,Larry Holzworth,云锦凤.新麦草在内蒙古干旱草原的建植试验.中国草地,1994(5):12-14Gu A L,Larry Holzworth,Yun J F.Establishment of two entries ofPsathyosrtahcysjunceathearidandsemi—arid Areasin Inner Mongolia.Grassland of China,1994(5):12-14
[5]于晓丹,张蕴薇.新麦草研究进展.草业与畜牧,2010(1):5-8,13YuXD,Zhang Y W.ResearchprogressofPsathyrostachys juncea.Prataculture&Animal Husbandry,2010(1):5-8,13
[6]赖勇,王晋民,任龙,朱慧琴,马辉,王金贵,巨霞,李宗仁.大麦SSR标记遗传多样性及连锁不平衡分析.核农学报,2016,30(10):1889-1897Lai Y,Wang J M,Ren L,Zhu H Q,Ma H,Wang J G,Ju X,LiZR.Geneticdiversityandlinkagedisequilibriumanalysis ofbarleyusingSSRmarkers.JournalofNuclear Agricultural Sciences,2016,30(10):1889-1897
[7]刘永财,孟林,张国芳,毛培春,张德罡.新麦草种质遗传多样性的ISSR分析.华北农学报,2009,24(5):107-112Liu Y C,Meng L,Zhang G F,Mao P C,Zhang D G.Genetic diversityanalysisof15Psathyrostachysjunceagermplasm resources by ISSR molecular marker.Acta Agriculturae BorealiSinica,2009,24(5):107-112
[8]Wei J Z,Campbell W F,Wang R R C.Genetic variability in Russian wildrye(Psathyrostachys juncea)assessed by RAPD.Genetic Resources and Crop Evolution,1997,44(2):117-125
[9]罗兵,孙海燕,徐港明,扬志刚,沈宗根,顾雯雯,高阳,郑佳亮.SSR分子标记研究进展.安徽农业科学,2013,41(12):5210-5212,5246LuoB,SunH Y,XuGM,YangZG,ShenZG,Gu W W,Gao Y,Zheng J L.Research progress of SSR molecular marker.Journal of Anhui Agricultural Sciences,2013,41(12):5210-5212,5246
[10]王娟,杜建材,王照兰,刘佳月,李青丰.36份苜蓿种质材料遗传多样性的SSR分子标记.中国草地学报,2016,38(2):20-25Wang J,Du J C,Wang Z L,Liu J Y,Li Q F.Genetic diversity analysis of 36 Alfalfa accessions based on SSR markers.Chinese Journal of Grassland,2016,38(2):20-25
[11]史晓红,郭平仲.分子标记与小麦群体遗传结构分析.首都师范大学学报:自然科学版,2000,21(1):59-65Shi X H,Guo P Z.Molecular marker and analysis of population genetic structure in wheat.Journal of Capital Normal University:Natural Science Edition,2000,21(1):59-65
[12]Liu Z W,Biyashev R M,Saghai Maroof M A.Development of simple sequence repeat DNA markers and their integration into a barley linkage map.Theoretical and Applied Genetics,1996,93:869-876
[13]Strussá,Plieske D J.The use of microsatellite markers for detection of genetic diversity in barley populations.Theoretical and Applied Genetics,1998,97:30
[14]Lakew B R,Henry J,Eglinton J,Baum M,Ceccarelli S,GrandoS.SSRanalysisofintrogressionofdroughttolerance from the genome of Hordeum spontaneum into cultivated barley(Hordeum vulgare subsp. vulgare).Euphytica,2013,191:231-243
[15]Ramsay L,Macaulay M.A simple sequence repeat-based linkage map of barley.Genetics,2000,156:1997-2005
[16]Varshney R K,Marcel T C,Ramsay L,Russell J,Roder M S,SteinN,WaughR,LangridgeP,NiksRE,Graner A.Ahigh density barley microsatellite consensus map with 775 SSR loci.Theoretical and Applied Genetics,2007,114:1091-1103
[17]郭培国,刘文杰,李海洋,王直亮,夏岩石,李荣华.一种快速有效检测SSR标记的非变性聚丙烯酰胺凝胶的银染方法.广州大学学报:自然科学版,2016,15(4):8-12,49Guo P G,Liu W J,Li H Y,Wang Z L,Xia Y S,Li R H.A rapid and effective method of silver staining for detecting SSR markers innondenaturingpolyacrylamidegels.JournalofGuangzhou University:Natural Science Edition,2016,15(4):8-12,49
[18]Evanno G,Regnaut S,Goudet J.Detecting the number of clustersofindividualsusingthesoftwareSTRUCTURE:a simulation study.Molecular Ecology,2005,14(8):2611-2620
[19]程雪妮,王颖,庞玉辉,陈新宏,武军,赵继新.小麦SSR和EST-SSR引物对无芒雀麦的通用性分析.植物科学学报,2014,32(1):27-33ChengXN,Wang Y,Pang YH,ChenXH,WuJ,ZhaoJ X.Primer transferability analysis on SSR and EST-SSR markers fromTriticumaestivumtoBromusinermis.PlantScience Journal,2014,32(1):27-33
[20]宿俊吉,柴守诚,刘伟华,杨欣明,李立会.普通小麦SSR和EST-SSR引物对冰草通用性的比较分析.西北植物学报,2007,27(7):1311-1316SuJJ,ChaiSC,Liu WH,YangXM,LiLH.Comparison oftransferabilityofcommonwheatSSRsandEST-SSRsto AgropyroncristatumL.ActaBotanicaBoreali-Occidentalia Sinica,2007,27(7):1311-1316
[21]邹玉婷.小麦EST-SSR标记对小麦及其近缘种属的遗传多样性研究.成都:四川农业大学,2010Zou Y T.Genetic diversity among triticeaes species as revealed bywheatEST-SSRmarker.Chengdu:Sichuan Agriculture University,2010
[22]张成林,郭志慧,张新全,白史且,张昌兵,李平,马啸.利用SSR标记对垂穗披碱草和老芒麦进行物种鉴定和遗传变异分析.植物遗传资源学报,2016,17(3):416-422ZhangCL,GuoZH,ZhangXQ,BaiSQ,ZhangCB,LiP,MaX.Ananalysisofspeciesidentificationandgenetic divergence of drooping wildrye(Elymus nutans)and siberian wildrye(E.sibiricus)by SSR markers. Journal of Plant Genetic Resources,2016,17(3):416-422
[23]苏龙,徐志健,乔卫华,郑晓明,梁云涛,丁膺宾,刘莎,齐兰,公婷婷,梁新霞,王君瑞,张丽芳,程云连,兰进好,杨庆文.广西药用野生稻遗传多样性分析及SSR引物数量对遗传多样性结果的影响研究.植物遗传资源学报,2017,18(4):603-610Su L,Xu Z J,Qiao W H,Zheng X M,Liang Y T,Ding Y B,LiuS,QiL,Gong T T,LiangXX,WangJR,ZhangLF,Cheng YL,LanJH,YangQ W.Studyongeneticdiversity of Oryza officinalis in Guangxi province and the effect of the number of SSR primers on genetic diversity analysis.Journal of Plant Genetic Resources,2017,18(4):603-610
[24]章秋平,刘威生,刘硕,魏潇,刘宁.杏遗传多样性评价所需SSR等位变异数目估计.果树学报,2015,32(2):186-191ZhangQP,Liu WS,LiuS,WeiX,LiuN.Anestimationof SSR alleles numbers for analyzing genetic diversity of apricot.Journal of Fruit Science,2015,32(2):186-191
[25]张晨,云岚,白亚利,程鹏飞,赵彦.新麦草种质资源的SSR遗传多样性分析.中国草地学报,2017,39(5):24-30Zhang C,Yun L,Bai Y L,Cheng P F,Zhao Y.Genetic diversity analysisof28Psathyrostachysjunceagermplasmresources based on SSR molecular markers.Chinese Journal of Grassland,2017,39(5):24-30
[26]杭焱,金燕,卢宝荣.濒危植物华山新麦草(Psathyrostachys huashanica)的遗传多样性及其保护.复旦学报:自然科学版,2004,43(2):260-266Hang Y,Jin Y,LuBR.Geneticdiversityoftheendangered species PsathyrostachyshuashanicainChinaanditsstrategic conservation.JournalofFudanUniversity:NaturalScience Edition,2004,43(2):260-266
[27]张云,龙云星,奎丽梅,徐高峰,涂建,辜琼瑶,李华慧,黄平,卢义宣.我国水稻两用核不育系SSR遗传多样性分析.分子植物育种,2017,15(7):2836-2846Zhang Y,Long Y X,Kui L M,Xu G F,Tu J,Gu Q Y,Li H H,Huang P,Lu Y X.SSR analysis on genetic diversity of rice dual-usenuclearmalesterilelinesinChina.MolecularPlant Breeding,2017,15(7):2836-2846
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