摘要
家蚕品种秋丰×白玉是一对耐氟性较强的夏秋用蚕品种,其中以亲本白玉A的耐氟性最强。以白玉A与耐氟性较弱的皓月B为亲本组配各种杂交世代进行耐氟性的遗传分析,结果表明在一定浓度的氟化物添食范围内,其耐氟性遗传呈显性遗传,受主效基因控制,并且具有部分伴性遗传效应和明显的杂种优势。利用SSR分子标记对耐氟性状进行连锁分析,初步确定耐氟主效基因与第12连锁群中的2个标记(chr12-2990-1和chr12-2990-2)紧密连锁。
Silkworm variety Qiufeng×Baiyu for summer and autumn using has strong fluoride tolerance, of which the parent Baiyu A has the strongest fluoride tolerance. According to genetic analysis on fluoride tolerance of various hybrid generations of Baiyu A and Haoyue B with low fluoride tolerance, fluoride tolerance inheritance is a dominant inheritance controlled by major genes and has partial sex-linked inheritance effect and obvious heterosis within certain concentration of fluoride. It can be preliminarily determined that the fluorine-tolerant major gene is closely linked to two markers(chr12-2990-1 and chr12-2990-2) on the 12 th linkage group based on the linkage analysis on fluoride tolerance with the help of the SSR molecular markers.
引文
[1] 林健荣,钟生泉.家蚕抗氟性遗传研究:Ⅱ.抗氟性遗传成分分析[J].蚕业科学,1990,16(1):25-28
[2] 魏兆军,王章娥,张志芳.家蚕耐氟性状基因效应分析[J].蚕业科学,2001,27(2):158-161
[3] 刘庆信,牟志美,刘训理,等.家蚕抗氟性双列杂交遗传分析[J].山东农业大学学报,1995,26(3):323-326
[4] 刘庆信,刘训理,张万英,等.家蚕抗氟性选择效果研究[J].蚕业科学,1998,24(1):58-59
[5] 林昌麒,糜懿殿,姚勤,等.家蚕耐氟遗传初探[J].江苏蚕业,1997(2):7-10
[6] 林昌麒,糜懿殿,姚勤,等.家蚕耐氟显性主效基因的发现[J].蚕业科学,1997,23(4):237-239
[7] 白会钗,徐安英,李木旺,等.利用SSR标记对家蚕耐氟基因进行连锁定位分析[J].蚕业科学,2008,34(2):191-196
[8] 章佩祯,季平,沈雪华,等.夏秋用蚕品种秋丰×白玉的育成[J].蚕业科学,1994,20(1):17-25
[9] 潘新平,林芳华,戴建一,等.新系秋丰×白玉的选育与推广[J].中国蚕业,2004,25(2):80-81
[10] 王夏英.薪杭×白云和秋丰×白玉推广应用效果评价[J].中国蚕业,2007,28(1):69-70
[11] 祝新荣,何克荣,柳新菊,等.雄蚕新品种“秋丰×平28”的育成[J].蚕业科学,2008,34(1):45-49
[12] 徐安英,林昌麒,钱荷英,等.耐家蚕核型多角体病毒病蚕品种“华康2号”的育成[J].蚕业科学,2013,39(2):275-282
[13] 中国农业科学院蚕业研究所.家蚕遗传育种学[M].北京:科学出版社,1981:311-312
[14] 林健荣.家蚕抗氟性遗传的研究:Ⅰ.杂种优势和配合力分析[J].蚕业科学,1989,15(1):31-36
[15] MIAO X X,XU S J,LI M H,et al.Simple sequence repeat based consensus linkage map of Bombyx mori[J].Proc Natl Acad Sci,2005,102(45):16303-16308
[16] 王亚馥,戴灼华.遗传学[M].北京:高等教育出版社,1999:1-18