化生平合剂对幽门结扎型大鼠胃黏膜损伤的保护作用
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摘要
目的研究化生平合剂对幽门结扎型胃溃疡大鼠胃液分泌、胃泌素(GAS)和白介素-8(IL-8)的影响及对胃黏膜损伤的修复作用。方法将50只雄性SD大鼠随机分为模型组、化生平合剂高、中、低剂量组和阳性对照组,各组大鼠给药1周后用幽门结扎法进行造模,5h后观察大鼠胃液分泌及胃组织溃疡情况,分离血清,用放射免疫法测定GAS及IL-8的含量。结果化生平合剂高、中、低剂量组均能减轻幽门结扎型大鼠的胃黏膜损伤程度。与模型组相比,大鼠胃液量、胃酸排出量、血清GAS及IL-8的水平均有所降低。结论化生平合剂对幽门结扎型大鼠胃黏膜损伤具有保护修复作用,其机制与其降低胃酸排出量及抑制胃黏膜损伤因子有关。
Objective To study the effects of Huashengping Mixture on gastric secretion,gastrin(GAS),interleukin-8(IL-8)and repair of gastric mucosal damage in rats with gastric ulcer induced by pylorus ligation.Methods 50 male SD rats were randomly divided into model group,high,middle and low doses of Huashengping Mixture group and positive control group.The rats in each group were given pylorus ligation for 1week.The gastric juice secretion and gastric ulcer were observed after 5h.Serum levels of GAS and IL-8were measured by radioimmunoassay.Results Huashengping Mixture high,middle and low dose groups can reduce gastric mucosal damage in pylorus ligation rats,and comparing with the model group,the gastric juice volume,gastric acid secretion,serum GAS and IL-8levels were also reduced.Conclusion Huashengping Mixture has a significant therapeutic effect on gastric ulcer in rats with pylorus ligation,and its mechanism is related to reducing gastric acid secretion and inhibiting gastric mucosal injury factors.
Objective To study the effects of Huashengping Mixture on gastric secretion,gastrin(GAS),interleukin-8(IL-8)and repair of gastric mucosal damage in rats with gastric ulcer induced by pylorus ligation.Methods 50 male SD rats were randomly divided into model group,high,middle and low doses of Huashengping Mixture group and positive control group.The rats in each group were given pylorus ligation for 1week.The gastric juice secretion and gastric ulcer were observed after 5h.Serum levels of GAS and IL-8were measured by radioimmunoassay.Results Huashengping Mixture high,middle and low dose groups can reduce gastric mucosal damage in pylorus ligation rats,and comparing with the model group,the gastric juice volume,gastric acid secretion,serum GAS and IL-8levels were also reduced.Conclusion Huashengping Mixture has a significant therapeutic effect on gastric ulcer in rats with pylorus ligation,and its mechanism is related to reducing gastric acid secretion and inhibiting gastric mucosal injury factors.
引文
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[24]Barrow A D,Palarasah Y,Bugatti M,et al.OSCAR is a receptor for surfactant protein D that activates TNF-αrelease from human CCR2+inflammatory monocytes[J].J Immunol,2015,194:3317-3326.
[25]张敏,李强,孔古娅.三九胃泰颗粒治疗实验性大鼠急性胃炎的药理研究[J].现代消化及介入诊疗杂志,2000,5(4):28-32.
[26]刘密,常小荣,严洁,等.艾灸预处理对应激性胃黏膜损伤大鼠血清IL-1β、TNF-α和IL-10的影响[J].中国中医急症,2011,20(6):906-908.
[27]Xue X,Chen Q,Zhao G,et al.The overexpression of TGF-βand CCN2in intrauterine adhesions involves the NF-κB signaling pathway[J].PLoS One,2015,10(12):e0146159.
[2]蔡艳,车晓侠,赵宇,等.化生平胶囊对大鼠胃溃疡的疗效研究[J].西北药学杂志,2015,30(4):373-376.
[3]王木成,弥希峰,许婷婷,等.左旋泮托拉唑钠对幽门结扎大鼠胃溃疡模型的治疗作用探讨[J].中国实用医药,2017,12(29):195-196.
[4]安秀峰,索菲娅,卢帅.孜然总黄酮抗实验性胃溃疡作用及机制研究[J].中国病理生理杂志,2017,33(7):1313-1316.
[5]杨永杰,王化河,王强.丹参对大鼠急性胃粘膜损伤保护作用的研究[J].江苏中医药,2004,25(7):54-55.
[6]陈军,鲁磊,高圣华.丹参注射液对胃溃疡患者氧化应激状态的影响[J].2014,12(17):45-46.
[7]程正位,熊建光,吴娟.复方丹参注射液对大鼠应激性胃黏膜损伤的影响[J].咸宁学院学报:医学版,2007,21(5):382-384.
[8]张会存,史瑞,李健,等.黄芪、三七对萎缩性胃炎大鼠胃黏膜HSP70及其转录因子GAF表达的影响[J].世界华人消化杂志,2013,21(7):559-566.
[9]李汉荣,王志成,杨琪,等.黄芪与三七协同治疗胃溃疡的临床观察[J].中国医药指南,2013,11(4):603-605.
[10]鲁占彪,王美梅,陈丽梅.黄连素结合三联疗法根除幽门螺杆菌感染的临床研究[J].中国现代药物应用,2013,7(4):84-85.
[11]梅刚,袁常珍.黄连素治疗胃溃疡的临床应用分析[J].中西医结合心血管病杂志,2016,4(12):58-59.
[12]张亚妮,雷剑虹.加味黄连解毒汤治疗幽门螺杆菌阳性胃溃疡疗效及对血清炎性因子水平影响[J].现代中西医结合杂志,2017,26(29):3270-3271,3280.
[13]林福林,杨昌云,杨薇薇,等.中药白芨的现代研究概况[J].中国医院药学杂志,2013,33(7):571-573.
[14]付瑞娟.白芨多糖对小鼠乙酸型胃溃疡治疗作用的探究[D].西安:陕西师范大学,2016.
[15]赵云生,毛福英,赵启鹏,等.甘草多糖抗炎与抗胃溃疡作用研究[J].亚太传统医药,2015,11(9):12-14.
[16]田莉,高晓黎.甘草的抗肿瘤作用[J].西北药学杂志,2004,19(3):133-135.
[17]王桂英,李振彬,石建喜,等.虎杖对消炎痛致大鼠胃溃疡的保护作用[J].河北中医药学报,2001,16(1):35-36.
[18]傅志泉,雍定国,周智林,等.虎杖口服液抗胃出血及抗胃溃疡作用的实验研究[J].浙江中医学院学报,2004,28(4):60-61.
[19]蔡永敏,徐彦飞,陈丽平,等.治疗胃溃疡相关中药应用频次研究[J].中国中西医结合杂志,2017,37(8):917-921.
[20]Correa P.A human model of gastric carcinogenesis[J].Cancer Res,1988,48:3554-3560.
[21]Silberg D G,Sullivan J,Kang E,et al.Cdx2ectopic expression induces gastric intestinal metaplasia in transgenic mice[J].Gastroenterology,2002,122(3):689-696.
[22]吴大威,王子娟,曹伟琪.药物治疗消化性溃疡进展[J].齐齐哈尔医学院学报,2011,32(2):258-259.
[23]阮灯甲.胃炎I号对慢性萎缩性胃炎大鼠血清胃泌素和血浆胃动素的调控作用[D].广州:广州中医药大学,2010.
[24]Barrow A D,Palarasah Y,Bugatti M,et al.OSCAR is a receptor for surfactant protein D that activates TNF-αrelease from human CCR2+inflammatory monocytes[J].J Immunol,2015,194:3317-3326.
[25]张敏,李强,孔古娅.三九胃泰颗粒治疗实验性大鼠急性胃炎的药理研究[J].现代消化及介入诊疗杂志,2000,5(4):28-32.
[26]刘密,常小荣,严洁,等.艾灸预处理对应激性胃黏膜损伤大鼠血清IL-1β、TNF-α和IL-10的影响[J].中国中医急症,2011,20(6):906-908.
[27]Xue X,Chen Q,Zhao G,et al.The overexpression of TGF-βand CCN2in intrauterine adhesions involves the NF-κB signaling pathway[J].PLoS One,2015,10(12):e0146159.
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