低温胁迫下高粱幼苗对外源脯氨酸的响应
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摘要
以冷敏高粱晋303为试验材料,幼苗三叶期用不同浓度的脯氨酸处理后, 2～4℃低温胁迫,测定相关生理指标,探讨外源脯氨酸处理对高粱幼苗耐冷性的影响。结果表明,外源脯氨酸处理可以提高低温胁迫下高粱叶片SOD、POD的活性,分别在3 mmol·L~(-1)和5 mmol·L~(-1)浓度脯氨酸的处理提高幅度最大。外源脯氨酸处理能降低低温胁迫下高粱叶片MDA的含量,以5 mmol·L~(-1)脯氨酸的处理效果最好。外源脯氨酸处理对叶片可溶性糖含量的增加也有作用,在本试验脯氨酸浓度设置范围内,可溶性糖含量随处理浓度的增加而增加。外源脯氨酸处理可以维持高粱幼苗中较高的叶绿素含量,喷施5 mmol·L~(-1)浓度脯氨酸时幼叶叶绿素含量最高。说明外源脯氨酸处理可有效缓解低温胁迫对高粱幼苗的伤害,提高幼苗的耐冷能力。
Using cold-sensitive sorghum Jin 303 as experimental material, different concentrations of proline(Pro) were used to treat sorghum seedlings at three-leaf stage, and then low temperature stress was carried out at 2-4 ℃. The effects of exogenous Pro treatment on cold tolerance of sorghum seedlings were studied by measuring physiological indexes. The results showed that exogenous Pro treatment could increase the activity of SOD and POD in sorghum leaves under low temperature stress, the highest increase was achieved in proline treatment at 3 mmol·L~(-1) and 5 mmol·L~(-1) concentrations respectively. It also reduced MDA content in Sorghum leaves under low temperature stress, and the best performance was obtained at 5 mmol·L~(-1). Besides, exogenous Pro treatment also had an effect on the increase of soluble sugar content in leaves. Within the concentration setting range of Pro in this experiment, the soluble sugar content increased with the increase of treatment concentration. Exogenous proline treatment could maintain high chlorophyll content in Sorghum seedlings, and the chlorophyll content in young leaves was the highest when proline was sprayed at 5 mol·L~(-1) concentration. This indicated that exogenous proline treatment could effectively alleviate the damage to Sorghum Seedlings under low temperature stress and improve the cold tolerance of seedlings.
Using cold-sensitive sorghum Jin 303 as experimental material, different concentrations of proline(Pro) were used to treat sorghum seedlings at three-leaf stage, and then low temperature stress was carried out at 2-4 ℃. The effects of exogenous Pro treatment on cold tolerance of sorghum seedlings were studied by measuring physiological indexes. The results showed that exogenous Pro treatment could increase the activity of SOD and POD in sorghum leaves under low temperature stress, the highest increase was achieved in proline treatment at 3 mmol·L~(-1) and 5 mmol·L~(-1) concentrations respectively. It also reduced MDA content in Sorghum leaves under low temperature stress, and the best performance was obtained at 5 mmol·L~(-1). Besides, exogenous Pro treatment also had an effect on the increase of soluble sugar content in leaves. Within the concentration setting range of Pro in this experiment, the soluble sugar content increased with the increase of treatment concentration. Exogenous proline treatment could maintain high chlorophyll content in Sorghum seedlings, and the chlorophyll content in young leaves was the highest when proline was sprayed at 5 mol·L~(-1) concentration. This indicated that exogenous proline treatment could effectively alleviate the damage to Sorghum Seedlings under low temperature stress and improve the cold tolerance of seedlings.
引文
[1]张桂香,史红梅,张海燕.高粱耐冷种质的鉴定及利用评价[J].中国农学通报,2012,28(36):87-91.
[2]史红梅,张海燕,杨彬.低温胁迫对高粱幼苗脯氨酸积累的影响[J].山西农业科学,2014,42(9):945-947.
[3]史红梅,张海燕,杨彬,等.低温胁迫对高粱幼苗MDA含量、SOD和POD活性的影响[J].中国农学通报,2015,31(18):74-79.
[4]汤章城,吴亚华,王育启,等.游离脯氨酸与高粱苗的抗旱性[J].植物生理学通讯,1986(3):29-35.
[5]汤章城.逆境条件下植物脯氨酸的累积及其可能的意义[J].植物生理学通讯,1984(1):15-21.
[6]陈晓云,杨洪兵.外源脯氨酸对荞麦幼苗耐盐性的效应[J].中国农学通报,2012,28(36):92-95.
[7]巴青松,张根生,张典典.外源脯氨酸对镍胁迫下小麦幼苗生长的影响[J].麦类作物学报,2017,37(5):700-704.
[8]Ding Y C,Chang C R,Luo W,et al.High Potassium Aggravates the Oxidative Stress Inducedy by Magnesium Deflciency in Rice Leaves[J].Pedosphere,2008,18(3):316-327.
[9]上海植物生理学会.植物生理学试验手册[M].上海:上海科技出版社,1985.
[10]刘德立,禹邦超,余世明,等.超氧物歧化酶(SOD)与植物抗逆性的关系[J].华中师范大学学报(自然科学版),1993,27(1):83-85.
[11]王荣富.植物抗寒指标的种类及其应用[J].植物生理学通讯,1987(3):49-55.
[12]李荣富,梁莉,胡晓红.低温对杏花丙二醛含量及过氧化物酶与超氧化物歧化酶活性的影响[J].内蒙古农业科技,2005(6):29-30.
[13]蒋安,郭彦军,范彦,等.低温胁迫对墨西哥玉米幼苗抗寒性的影响[J].草业科学,2010,27(3):89-92.
[14]FRANCISCO J.SANCHEZ,MARIA MANZANARES,EUSEBIO F.DE ANDRES,et al.Turgor maintenance,osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress[J].Field Crops Research,1998,59(3):225-235.
[15]张桂莲,陈立云,张顺堂,等.抽穗开花期高温对水稻剑叶理化特性的影响[J].中国农业科学,2007,40(7):1345-1352.
[16]陈晓云,杨洪兵.外源脯氨酸对荞麦幼苗耐盐性的效应[J].中国农学通报,2012,28(36):92-95.
[17]郭启芳,马千全,孙灿,等.外源甜菜碱提高小麦幼苗抗盐性的研究[J].西北植物学报,2004,24(9):1680-1686.
[18]吉增宝,王进鑫,李继文,等.不同季节干旱及复水对刺槐幼苗可溶性糖含量的影响[J].西北植物学报,2009,29(7):1358-1363.
[2]史红梅,张海燕,杨彬.低温胁迫对高粱幼苗脯氨酸积累的影响[J].山西农业科学,2014,42(9):945-947.
[3]史红梅,张海燕,杨彬,等.低温胁迫对高粱幼苗MDA含量、SOD和POD活性的影响[J].中国农学通报,2015,31(18):74-79.
[4]汤章城,吴亚华,王育启,等.游离脯氨酸与高粱苗的抗旱性[J].植物生理学通讯,1986(3):29-35.
[5]汤章城.逆境条件下植物脯氨酸的累积及其可能的意义[J].植物生理学通讯,1984(1):15-21.
[6]陈晓云,杨洪兵.外源脯氨酸对荞麦幼苗耐盐性的效应[J].中国农学通报,2012,28(36):92-95.
[7]巴青松,张根生,张典典.外源脯氨酸对镍胁迫下小麦幼苗生长的影响[J].麦类作物学报,2017,37(5):700-704.
[8]Ding Y C,Chang C R,Luo W,et al.High Potassium Aggravates the Oxidative Stress Inducedy by Magnesium Deflciency in Rice Leaves[J].Pedosphere,2008,18(3):316-327.
[9]上海植物生理学会.植物生理学试验手册[M].上海:上海科技出版社,1985.
[10]刘德立,禹邦超,余世明,等.超氧物歧化酶(SOD)与植物抗逆性的关系[J].华中师范大学学报(自然科学版),1993,27(1):83-85.
[11]王荣富.植物抗寒指标的种类及其应用[J].植物生理学通讯,1987(3):49-55.
[12]李荣富,梁莉,胡晓红.低温对杏花丙二醛含量及过氧化物酶与超氧化物歧化酶活性的影响[J].内蒙古农业科技,2005(6):29-30.
[13]蒋安,郭彦军,范彦,等.低温胁迫对墨西哥玉米幼苗抗寒性的影响[J].草业科学,2010,27(3):89-92.
[14]FRANCISCO J.SANCHEZ,MARIA MANZANARES,EUSEBIO F.DE ANDRES,et al.Turgor maintenance,osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress[J].Field Crops Research,1998,59(3):225-235.
[15]张桂莲,陈立云,张顺堂,等.抽穗开花期高温对水稻剑叶理化特性的影响[J].中国农业科学,2007,40(7):1345-1352.
[16]陈晓云,杨洪兵.外源脯氨酸对荞麦幼苗耐盐性的效应[J].中国农学通报,2012,28(36):92-95.
[17]郭启芳,马千全,孙灿,等.外源甜菜碱提高小麦幼苗抗盐性的研究[J].西北植物学报,2004,24(9):1680-1686.
[18]吉增宝,王进鑫,李继文,等.不同季节干旱及复水对刺槐幼苗可溶性糖含量的影响[J].西北植物学报,2009,29(7):1358-1363.