Cu~(2+)、Zn~(2+)、Cd~(2+)对黑水虻幼虫生长的影响及在虫体和虫粪的积累
|
摘要
为研究不同金属离子对黑水虻幼虫生长的影响及在虫体和虫粪中的积累分布规律,通过在黑水虻幼虫饲料中加入不同浓度的Cu~(2+)、Zn~(2+)、Cd~(2+),分析观测了幼虫增重、虫体和虫粪中金属含量等情况。结果表明,适量的Cu~(2+)、Zn~(2+)、Cd~(2+)可以促进黑水虻幼虫的生长,Zn~(2+)浓度在400 mg/kg时黑水虻幼虫的体重最高。Cu~(2+)、Zn~(2+)、Cd~(2+)在黑水虻幼虫体内和虫粪中的残留随着饲料中Cu~(2+)、Zn~(2+)、Cd~(2+)浓度的升高而增加。Cu~(2+)、Zn~(2+)在虫体中的残留比例都小于50%,大部分残留在虫粪中,Cd~(2+)在10、20、40 mg/kg处理中虫体的残留比例大于50%,最高达到了91.3%,而在80、160 mg/kg处理中虫体残留量比例小于50%。各重金属在虫粪中的残留主要以残渣态存在,且各形态的含量顺序在高浓度处理中都为残渣态>酸溶态>水溶态>碱溶态。说明饲料中适量金属离子的添加有利于黑水虻幼虫生长,但过量的添加会抑制黑水虻幼虫生长,并造成金属离子的积累。
In order to study the effects of metal ions on the growth of black soldier fly larvae and the accumulation distribution in larvae and feces, the larvae breeding experiments with different concentrations of Cu~(2+), Zn~(2+), Cd~(2+) in the feed were done by analysising the indexes of larval weight, metal content in larvae and feces. The results showed that the appropriate amount of Cu~(2+), Zn~(2+), Cd~(2+) could promote the growth of the larvae. The larvae weight was the highest under concentration of Zn~( 2+)400 mg/kg. Residues of Cu~(2+), Zn~(2+) and Cd~(2+) in the larvae and feces were increased with increasing of the concentration of Cu~(2+), Zn~(2+), Cd~(2+) in the feed. The residues of Cu~(2+) and Zn~(2+) in larvae were less than 50%, but most of them remained in feces. The Cd~(2+) residue ratio of larvae was more than 50%, the highest ratio was 91.3% under 10, 20 and 40 mg/kg treatments, while the Cd~(2+) residue ratio of larvae was less than 50% under 80 and 160 mg/kg treatments. The residues of heavy metals in feces mainly existed in residual state, and the order of contents in each form was residual state > acid soluble state > water soluble state > alkali soluble state under high concentration. These indicated that the addition of appropriate metal ions in feed was beneficial for the growth of the larvae, but excessive addition would inhibit the larvae growth and cause the accumulation of metal ions.
In order to study the effects of metal ions on the growth of black soldier fly larvae and the accumulation distribution in larvae and feces, the larvae breeding experiments with different concentrations of Cu~(2+), Zn~(2+), Cd~(2+) in the feed were done by analysising the indexes of larval weight, metal content in larvae and feces. The results showed that the appropriate amount of Cu~(2+), Zn~(2+), Cd~(2+) could promote the growth of the larvae. The larvae weight was the highest under concentration of Zn~( 2+)400 mg/kg. Residues of Cu~(2+), Zn~(2+) and Cd~(2+) in the larvae and feces were increased with increasing of the concentration of Cu~(2+), Zn~(2+), Cd~(2+) in the feed. The residues of Cu~(2+) and Zn~(2+) in larvae were less than 50%, but most of them remained in feces. The Cd~(2+) residue ratio of larvae was more than 50%, the highest ratio was 91.3% under 10, 20 and 40 mg/kg treatments, while the Cd~(2+) residue ratio of larvae was less than 50% under 80 and 160 mg/kg treatments. The residues of heavy metals in feces mainly existed in residual state, and the order of contents in each form was residual state > acid soluble state > water soluble state > alkali soluble state under high concentration. These indicated that the addition of appropriate metal ions in feed was beneficial for the growth of the larvae, but excessive addition would inhibit the larvae growth and cause the accumulation of metal ions.
引文
Cao JH. Effects of Trace Nutrient Injection on the Growth of Grazing Livestock in Qinghai Tibet Plateau [D]. Xining: Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 2006. [曹俊虎. 微量元素营养针剂对青藏高原放牧家畜生长的影响 [D]. 西宁: 中国科学院西北高原生物研究所, 2006]
Chen HM. The Preliminary Study on Existing Forms of Cadmium in Organic Fertilizers and Their Bioavailability [D]. Chongqing: Southwest University, 2007. [陈洪敏. 有机肥中镉的赋存状态与生物活性初探 [D]. 重庆: 西南大学, 2007]
Chen X, Wang XR, Ji R. Bioaccumulation and oxidative stresses of cadmium in earthworm Eisenia foetida in a paddy soil [J]. Journal of Agro-Environment Science, 2015, 34 (8): 1464-1469. [陈娴, 王晓蓉, 季荣. 蚯蚓对水稻土中Cd的富集及其氧化应激 [J]. 农业环境科学学报, 2015, 34 (8): 1464-1469]
Gao Q, Liu XH, Li K, et al. Research progress in the development of high value-added products of Hermetia illucens [J]. Journal of Anhui Agricultural Sciences, 2016, 44 (34): 102-104. [高俏, 刘馨桧, 李逵, 等. 亮斑扁角水虻高附加值产品开发的研究进展 [J]. 安徽农业科学, 2016, 44 (34): 102-104]
General Administration of Quality Supervision, Inspection and Quarantine of PRC. GB 13078-2001 Hygienic Standard for Feeds [S]. Beijing: China Standards Press, 2001. [中华人民共和国国家质量监督检验检疫总局. GB 13078-2001饲料卫生标准 [S]. 北京: 中国标准出版社, 2001]
General Administration of Quality Supervision, Inspection and Quarantine of PRC. GB 26419-2010 Limited Contents of Copper in Feeds [S]. Beijing: China Standards Press, 2011. [中华人民共和国国家质量监督检验检疫总局. GB 26419-2010饲料中铜的允许量 [S]. 北京: 中国标准出版社, 2011]
Geng W, Hu L, Cui JY, et al. Biogas energy potential for livestock manure and gross control of animal feeding in region level of China [J]. Transactions of the Chinese Society of Agricultural Engineering, 2013, 29 (1): 171-179. [耿维, 胡林, 崔建宇, 等. 中国区域畜禽粪便能源潜力及总量控制研究 [J]. 农业工程学报, 2013, 29 (1): 171-179]
Hendriks AJ, Ma WC. Modelling and monitoring organochlorine and heavy metal accumulation in soils, earthworms and shrews in Rhinedelta Floodplains [J]. Arch Environ. Contam. Toxicol, 1999, 37 (1): 70-77.
Huang YY, Chen GF, Liu B, et al. Content of Cu and Zn in pig feeds and their residual character in pig manures from intensive piggery in Guangxi [J]. Journal of Anhui Agricultural Sciences, 2012, 40 (17): 9280-9281, 9284. [黄玉溢, 陈桂芬, 刘斌, 等.广西集约化养殖猪饲料Cu和Zn含量及粪便Cu和Zn残留特性研究 [J]. 安徽农业科学, 2012, 40 (17): 9280-9281, 9284]
Hu MM, Wang Q, Yang YZ. Accumulation, distribution and excretion of heavy metals in insects [J]. Chinese Agricultural Science Bulletin, 2012, 28 (18): 213-217. [胡蒙蒙, 王青, 杨益众. 重金属在昆虫体内的累积、分布与排泄 [J]. 中国农学通报, 2012, 28 (18): 213-217]
Hu MM. Accumulation and Excretion Activities of Land and Cadmium in Spodoptera exigua and the Impact on the Population Increase [D]. Yangzhou: Yangzhou University, 2013. [胡蒙蒙. Pb2+和Cd2+在甜菜夜蛾体内的积累与排泄及对种群增长的影响 [D]. 扬州: 扬州大学, 2013]
Jiang P, Jin SY, Hao XZ, et al. Distribution characteristics of heavy metals in feeds, pig manures, soils and vegetables [J]. Journal of Agro-Environment Science, 2010, 29 (5): 942-947. [姜萍, 金盛杨, 郝秀珍, 等. 重金属在猪饲料一粪便一土壤一蔬菜中的分布特征研究 [J]. 农业环境科学学报, 2010, 29 (5): 942-947]
Jia WX, Wen J, Xu WL, et al. Content and fractionation of heavy metals in livestock manures in some urban areas of China [J]. Journal of Agro-Environment Science, 2016, 35 (4): 764-773. [贾武霞, 文炯, 许望龙, 等. 我国部分城市畜禽粪便中重金属含量及形态分布 [J]. 农业环境科学学报, 2016, 35 (4): 764-773]
Li GL, Wei SQ, Qing CL. Kinetic and thermodynamic of cadmium adsorption on humicacid [J]. Acta Pedologica Sinica, 2004, 41 (1): 74-80. [李光林, 魏世强, 青长乐. 镉在胡敏酸上的吸附动力学和热力学研究 [J]. 土壤学报, 2004, 41 (1): 74-80]
Li GL, Wei SQ, Qing CL, et al. Characteristics of absorption and desorption of cadmium on humicacids [J]. Journal of Agro-Environment Science, 2003, 22 (1): 34-37. [李光林, 魏世强, 青长乐, 等. 镉在腐植酸上的吸附与解吸特征研究 [J]. 农业环境科学学报, 2003, 22 (1): 34-37]
Lin Y, Ma J, Qin F. The structure distribution and prospect of China manure resource [J]. Chinese Agricultural Science Bulletin, 2012, 28 (32): 1-5. [林源, 马骥, 秦富. 中国畜禽粪便资源结构分布及发展展望 [J]. 中国农学通报, 2012, 28 (32): 1-5]
Liu HY, Yu GH, Xia Q. The research progress of Hermetia illucens [J]. Culture and Feed, 2015, 12: 4-7. [刘宏宇, 喻国辉, 夏嫱. 黑水虻研究进展 [J]. 养殖与饲料, 2015, 12: 4-7]
Li W, Li Q, Zheng LY, et al. Potential biodiesel and biogas production from corncob by anaerobic fermentation and black soldier fly [J]. Biore-source Technology, 2015, 194: 276-282.
Ministry of Agriculture of PRC. NY929-2005 Limited Contents of Zinc in Feeds [S]. Beijing: China Standards Press, 2005. [中华人民共和国农业部. NY 929-2005饲料中锌的允许量 [S]. 北京: 中国标准出版社, 2005]
Newton GL, BooramCV, Barker RW, et al. Dried Hermetia illucens larvae meal as a supplement for swine [J]. Journal of Animal Science, 1977, 44: 395-399.
Pan X, Han Z, Ben WW. Heavy metal contents in pig manure and pig feeds from intensive pig farms in Shandong Province, China [J]. Journal of Agro-Environment Science, 2013, 32 (1): 160-165. [潘寻, 韩哲, 贲伟伟. 山东省规模化猪场猪粪及配合饲料中重金属质量分数研究 [J]. 农业环境科学学报, 2013, 32 (1): 160-165]
Wang F, Qiu L, Shen YJ, et al. Investigation and analysis of heavy metal contents from livestock feed and manure in North China [J]. Transactions of the Chinese Society of Agricultural Engineering, 2015, 31 (5): 261-267. [王飞, 邱凌, 沈玉君, 等. 华北地区饲料和畜禽粪便中重金属质量分数调查分析 [J]. 农业工程学报, 2015, 31 (5): 261-267]
Wang YJ, Dou S, Li YD, et al. Speciation change of heavy metals during composting process of chicken manure [J]. Environmental Science, 2009, 30 (3): 913-917. [王玉军, 窦森, 李业东, 等. 鸡粪堆肥处理对重金属形态的影响 [J]. 环境科学, 2009, 30 (3): 913-917]
Xia Q, Dan JL, Zhu W, et al. Effects of zinc on the growth and development of black soldier fly [J]. Journal of Environmental Entomology, 2013, 35 (2): 294-299. [夏嫱, 但家立, 朱伟, 等. Zn2+胁迫对黑水虻生长发育的影响 [J]. 环境昆虫学报, 2013, 35 (2): 294-299]
Xia Q, Zhu W, Liao Y, et al. Migration of Cu2+ in black soldier flies and its effects on the development [J]. Journal of Zunyi Medical University, 2014, 37 (3): 300-303. [夏嫱, 朱伟, 廖业, 等. Cu2+在黑水虻体内迁移及对其发育影响 [J]. 遵义医学院学报, 2014, 37 (3): 300-303]
Yao LX, Huang LX, Jiang ZY, et al. Investigation of As, Cu and Zn species and concentrations in animal feeds [J]. Environmental Sciences, 2013, 34 (2): 732 -739. [姚丽贤, 黄连喜, 蒋宗勇, 等. 动物饲料中砷、铜和锌调查及分析 [J]. 环境科学, 2013, 34 (2): 732-739]
Zhang L. Research on the Method for Characterizing Heavy Metal Speciation in Manure and its Application [D]. Hangzhou: Zhejiang University, 2016. [张莉. 有机肥中重金属形态分析方法的研究与应用 [D]. 杭州: 浙江大学, 2016]
Zheng GD, Chen TB, Gao D, et al. Influence of high temperature aerobic composting treatment on the form of heavy metals in pig manure [J]. China Environmental Science, 2005, 25 (1): 6-9. [郑国砥, 陈同斌, 高定, 等. 好氧高温堆肥处理对猪粪中重金属形态的影响 [J]. 中国环境科学, 2005, 25 (1): 6-9]
Zhu JC, Li RH, Zhang ZQ, et al. Heavy metal contents in pig manure and feeds under intensive farming and potential hazard on farmlands in Shanxi Province, China [J]. Transactions of the Chinese Society for Agricultural Machinery, 2013, 44 (11): 98-105. [朱建春, 李荣华, 张增强, 等. 陕西规模化猪场猪粪与饲料重金属质量分数研究 [J]. 农业机械学报, 2013, 44 (11): 98-105]
Chen HM. The Preliminary Study on Existing Forms of Cadmium in Organic Fertilizers and Their Bioavailability [D]. Chongqing: Southwest University, 2007. [陈洪敏. 有机肥中镉的赋存状态与生物活性初探 [D]. 重庆: 西南大学, 2007]
Chen X, Wang XR, Ji R. Bioaccumulation and oxidative stresses of cadmium in earthworm Eisenia foetida in a paddy soil [J]. Journal of Agro-Environment Science, 2015, 34 (8): 1464-1469. [陈娴, 王晓蓉, 季荣. 蚯蚓对水稻土中Cd的富集及其氧化应激 [J]. 农业环境科学学报, 2015, 34 (8): 1464-1469]
Gao Q, Liu XH, Li K, et al. Research progress in the development of high value-added products of Hermetia illucens [J]. Journal of Anhui Agricultural Sciences, 2016, 44 (34): 102-104. [高俏, 刘馨桧, 李逵, 等. 亮斑扁角水虻高附加值产品开发的研究进展 [J]. 安徽农业科学, 2016, 44 (34): 102-104]
General Administration of Quality Supervision, Inspection and Quarantine of PRC. GB 13078-2001 Hygienic Standard for Feeds [S]. Beijing: China Standards Press, 2001. [中华人民共和国国家质量监督检验检疫总局. GB 13078-2001饲料卫生标准 [S]. 北京: 中国标准出版社, 2001]
General Administration of Quality Supervision, Inspection and Quarantine of PRC. GB 26419-2010 Limited Contents of Copper in Feeds [S]. Beijing: China Standards Press, 2011. [中华人民共和国国家质量监督检验检疫总局. GB 26419-2010饲料中铜的允许量 [S]. 北京: 中国标准出版社, 2011]
Geng W, Hu L, Cui JY, et al. Biogas energy potential for livestock manure and gross control of animal feeding in region level of China [J]. Transactions of the Chinese Society of Agricultural Engineering, 2013, 29 (1): 171-179. [耿维, 胡林, 崔建宇, 等. 中国区域畜禽粪便能源潜力及总量控制研究 [J]. 农业工程学报, 2013, 29 (1): 171-179]
Hendriks AJ, Ma WC. Modelling and monitoring organochlorine and heavy metal accumulation in soils, earthworms and shrews in Rhinedelta Floodplains [J]. Arch Environ. Contam. Toxicol, 1999, 37 (1): 70-77.
Huang YY, Chen GF, Liu B, et al. Content of Cu and Zn in pig feeds and their residual character in pig manures from intensive piggery in Guangxi [J]. Journal of Anhui Agricultural Sciences, 2012, 40 (17): 9280-9281, 9284. [黄玉溢, 陈桂芬, 刘斌, 等.广西集约化养殖猪饲料Cu和Zn含量及粪便Cu和Zn残留特性研究 [J]. 安徽农业科学, 2012, 40 (17): 9280-9281, 9284]
Hu MM, Wang Q, Yang YZ. Accumulation, distribution and excretion of heavy metals in insects [J]. Chinese Agricultural Science Bulletin, 2012, 28 (18): 213-217. [胡蒙蒙, 王青, 杨益众. 重金属在昆虫体内的累积、分布与排泄 [J]. 中国农学通报, 2012, 28 (18): 213-217]
Hu MM. Accumulation and Excretion Activities of Land and Cadmium in Spodoptera exigua and the Impact on the Population Increase [D]. Yangzhou: Yangzhou University, 2013. [胡蒙蒙. Pb2+和Cd2+在甜菜夜蛾体内的积累与排泄及对种群增长的影响 [D]. 扬州: 扬州大学, 2013]
Jiang P, Jin SY, Hao XZ, et al. Distribution characteristics of heavy metals in feeds, pig manures, soils and vegetables [J]. Journal of Agro-Environment Science, 2010, 29 (5): 942-947. [姜萍, 金盛杨, 郝秀珍, 等. 重金属在猪饲料一粪便一土壤一蔬菜中的分布特征研究 [J]. 农业环境科学学报, 2010, 29 (5): 942-947]
Jia WX, Wen J, Xu WL, et al. Content and fractionation of heavy metals in livestock manures in some urban areas of China [J]. Journal of Agro-Environment Science, 2016, 35 (4): 764-773. [贾武霞, 文炯, 许望龙, 等. 我国部分城市畜禽粪便中重金属含量及形态分布 [J]. 农业环境科学学报, 2016, 35 (4): 764-773]
Li GL, Wei SQ, Qing CL. Kinetic and thermodynamic of cadmium adsorption on humicacid [J]. Acta Pedologica Sinica, 2004, 41 (1): 74-80. [李光林, 魏世强, 青长乐. 镉在胡敏酸上的吸附动力学和热力学研究 [J]. 土壤学报, 2004, 41 (1): 74-80]
Li GL, Wei SQ, Qing CL, et al. Characteristics of absorption and desorption of cadmium on humicacids [J]. Journal of Agro-Environment Science, 2003, 22 (1): 34-37. [李光林, 魏世强, 青长乐, 等. 镉在腐植酸上的吸附与解吸特征研究 [J]. 农业环境科学学报, 2003, 22 (1): 34-37]
Lin Y, Ma J, Qin F. The structure distribution and prospect of China manure resource [J]. Chinese Agricultural Science Bulletin, 2012, 28 (32): 1-5. [林源, 马骥, 秦富. 中国畜禽粪便资源结构分布及发展展望 [J]. 中国农学通报, 2012, 28 (32): 1-5]
Liu HY, Yu GH, Xia Q. The research progress of Hermetia illucens [J]. Culture and Feed, 2015, 12: 4-7. [刘宏宇, 喻国辉, 夏嫱. 黑水虻研究进展 [J]. 养殖与饲料, 2015, 12: 4-7]
Li W, Li Q, Zheng LY, et al. Potential biodiesel and biogas production from corncob by anaerobic fermentation and black soldier fly [J]. Biore-source Technology, 2015, 194: 276-282.
Ministry of Agriculture of PRC. NY929-2005 Limited Contents of Zinc in Feeds [S]. Beijing: China Standards Press, 2005. [中华人民共和国农业部. NY 929-2005饲料中锌的允许量 [S]. 北京: 中国标准出版社, 2005]
Newton GL, BooramCV, Barker RW, et al. Dried Hermetia illucens larvae meal as a supplement for swine [J]. Journal of Animal Science, 1977, 44: 395-399.
Pan X, Han Z, Ben WW. Heavy metal contents in pig manure and pig feeds from intensive pig farms in Shandong Province, China [J]. Journal of Agro-Environment Science, 2013, 32 (1): 160-165. [潘寻, 韩哲, 贲伟伟. 山东省规模化猪场猪粪及配合饲料中重金属质量分数研究 [J]. 农业环境科学学报, 2013, 32 (1): 160-165]
Wang F, Qiu L, Shen YJ, et al. Investigation and analysis of heavy metal contents from livestock feed and manure in North China [J]. Transactions of the Chinese Society of Agricultural Engineering, 2015, 31 (5): 261-267. [王飞, 邱凌, 沈玉君, 等. 华北地区饲料和畜禽粪便中重金属质量分数调查分析 [J]. 农业工程学报, 2015, 31 (5): 261-267]
Wang YJ, Dou S, Li YD, et al. Speciation change of heavy metals during composting process of chicken manure [J]. Environmental Science, 2009, 30 (3): 913-917. [王玉军, 窦森, 李业东, 等. 鸡粪堆肥处理对重金属形态的影响 [J]. 环境科学, 2009, 30 (3): 913-917]
Xia Q, Dan JL, Zhu W, et al. Effects of zinc on the growth and development of black soldier fly [J]. Journal of Environmental Entomology, 2013, 35 (2): 294-299. [夏嫱, 但家立, 朱伟, 等. Zn2+胁迫对黑水虻生长发育的影响 [J]. 环境昆虫学报, 2013, 35 (2): 294-299]
Xia Q, Zhu W, Liao Y, et al. Migration of Cu2+ in black soldier flies and its effects on the development [J]. Journal of Zunyi Medical University, 2014, 37 (3): 300-303. [夏嫱, 朱伟, 廖业, 等. Cu2+在黑水虻体内迁移及对其发育影响 [J]. 遵义医学院学报, 2014, 37 (3): 300-303]
Yao LX, Huang LX, Jiang ZY, et al. Investigation of As, Cu and Zn species and concentrations in animal feeds [J]. Environmental Sciences, 2013, 34 (2): 732 -739. [姚丽贤, 黄连喜, 蒋宗勇, 等. 动物饲料中砷、铜和锌调查及分析 [J]. 环境科学, 2013, 34 (2): 732-739]
Zhang L. Research on the Method for Characterizing Heavy Metal Speciation in Manure and its Application [D]. Hangzhou: Zhejiang University, 2016. [张莉. 有机肥中重金属形态分析方法的研究与应用 [D]. 杭州: 浙江大学, 2016]
Zheng GD, Chen TB, Gao D, et al. Influence of high temperature aerobic composting treatment on the form of heavy metals in pig manure [J]. China Environmental Science, 2005, 25 (1): 6-9. [郑国砥, 陈同斌, 高定, 等. 好氧高温堆肥处理对猪粪中重金属形态的影响 [J]. 中国环境科学, 2005, 25 (1): 6-9]
Zhu JC, Li RH, Zhang ZQ, et al. Heavy metal contents in pig manure and feeds under intensive farming and potential hazard on farmlands in Shanxi Province, China [J]. Transactions of the Chinese Society for Agricultural Machinery, 2013, 44 (11): 98-105. [朱建春, 李荣华, 张增强, 等. 陕西规模化猪场猪粪与饲料重金属质量分数研究 [J]. 农业机械学报, 2013, 44 (11): 98-105]
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |