地格达-4味汤煎煮工艺考察
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摘要
目的:优化地格达-4味汤(DGD-4D)的煎煮工艺,为蒙药汤剂煎煮规范化研究提供参考。方法:以DGD-4D为模型药,比较了不同蒙药煎煮方法;采用HPLC测定汤剂中指标成分秦皮乙素、栀子苷、胡黄连苷Ⅰ和胡黄连苷Ⅱ的含量,以指标成分转移率和浸膏得率为指标,在单因素试验基础上,采用星点设计-效应面法优选DGD-4D的煎煮工艺,使用Design-Expert8. 0. 6软件进行回归模型拟合,建立工艺参数预测模型,并对最优工艺进行验证。结果:确定DGD-4D最佳煎煮条件为加40倍量水,煎煮17 min,煎煮1次;秦皮乙素、栀子苷、胡黄连苷Ⅰ和胡黄连苷Ⅱ转移率以及浸膏得率分别为70. 01%,94. 11%,61. 23%,92. 32%,32. 89%。结论:建立了DGD-4D的最佳煎煮方法,对于提高蒙药制剂水平以及保证其临床疗效的一致性具有重要参考意义。
Objective: To optimize the decoction process of Digda-4 decoction( DGD-4 D),and provide reference for the standardization study of decoction of Mongolian medicine decoction. Method: Taking DGD-4 D as model drug,different decoction methods of Mongolian medicine were compared,HPLC was used to determine contents of aesculetin,geniposide,picroside Ⅰ and picroside Ⅱ. On the basis of single factor tests,central composite design-response surface methodology was adopted to optimize the decoction process of DGD-4 D with transfer rates of 4 components and dry extract rate as indexes,regression model fitting was carried out by DesignExpert 8. 0. 6 software, prediction model of process parameters was established, and the optimal process was verified. Result: The optimal decoction condition of DGD-4 D was determined to be adding 40 times the amount of water and decocting for 17 min, decocting once. Transfer rates of aesculetin, geniposide, picroside Ⅰ,picroside Ⅱ and dry extract rate were 70. 01%, 94. 11%, 61. 23%, 92. 32%, 32. 89%, respectively.Conclusion: The optimum decoction process of DGD-4 D is established,it has important reference significance for excavating,sorting,improving the level of Mongolian medicine preparations and ensuring the consistency of their clinical efficacy.
Objective: To optimize the decoction process of Digda-4 decoction( DGD-4 D),and provide reference for the standardization study of decoction of Mongolian medicine decoction. Method: Taking DGD-4 D as model drug,different decoction methods of Mongolian medicine were compared,HPLC was used to determine contents of aesculetin,geniposide,picroside Ⅰ and picroside Ⅱ. On the basis of single factor tests,central composite design-response surface methodology was adopted to optimize the decoction process of DGD-4 D with transfer rates of 4 components and dry extract rate as indexes,regression model fitting was carried out by DesignExpert 8. 0. 6 software, prediction model of process parameters was established, and the optimal process was verified. Result: The optimal decoction condition of DGD-4 D was determined to be adding 40 times the amount of water and decocting for 17 min, decocting once. Transfer rates of aesculetin, geniposide, picroside Ⅰ,picroside Ⅱ and dry extract rate were 70. 01%, 94. 11%, 61. 23%, 92. 32%, 32. 89%, respectively.Conclusion: The optimum decoction process of DGD-4 D is established,it has important reference significance for excavating,sorting,improving the level of Mongolian medicine preparations and ensuring the consistency of their clinical efficacy.
引文
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[10]曹捷,秦艳,尹成乐,等.紫花地丁化学成分及抗氧化活性[J].中国实验方剂学杂志,2013,19(21):77-81.
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[12] Kitano A,Saika S,Yamanaka O,et al. Genipin suppression of fibrogenic behaviors of theα-TN4 lens epithelial cell line[J]. J Cataract Refract Surg,2006,32(10):1727-1735.
[13]刘臻,张铭倩,朱虹.栝楼瞿麦汤对糖尿病肾病大鼠脂肪因子和足细胞nephrin的影响[J].中国实验方剂学杂志,2017,23(23):134-139.
[14]段晓颖,高卫芳,吴彩丽,等.均匀设计法优选胡黄连中胡黄连苷的提取工艺[J].中国药房,2011,22(39):3675-3676.
[15] Kumar V,Sood H,Chauhan R S. Optimization of a preparative RP-HPLC method for isolation and purification of picrosides in Picrorhiza kurroa[J]. J Plant Biochem Biot,2016,25(2):208-214.
[2]内蒙古自治区食品药品监督管理局.内蒙古蒙药制剂规范.第二册[M].呼和浩特:内蒙古人民出版社,2014:附录7-8.
[3]内蒙古自治区卫生厅.内蒙古蒙成药标准[M].呼和浩特:内蒙古科学技术出版社,1984:389-391.
[4]那生桑.蒙药制剂学[M].呼和浩特:内蒙古人民出版社,2012:203-208.
[5]巴根那.蒙医方剂学[M].呼和浩特:内蒙古人民出版社,2007:21-23.
[6]蒙医学编辑委员会.中国医学百科全书·蒙医学[M].上海:上海科学技术出版社,1992:238.
[7]白清云.中国医学百科全书·蒙医学(上)[M].呼和浩特:内蒙古科学技术出版社,1980:687.
[8]乌云.蒙药方剂大全[M].呼和浩特:内蒙古人民出版社,2006:1-2.
[9]田香,丁瑞雪,罗晓健,等.蒙药地格达-4味汤指纹图谱及多指标成分定量分析研究[J].中国中药杂志,2018,43(19):240-247.
[10]曹捷,秦艳,尹成乐,等.紫花地丁化学成分及抗氧化活性[J].中国实验方剂学杂志,2013,19(21):77-81.
[11]李鹏,郑璐,汪斌,等.胡黄连苷-Ⅱ原料药的定性与定量分析[J].中国实验方剂学杂志,2015,21(14):56-58.
[12] Kitano A,Saika S,Yamanaka O,et al. Genipin suppression of fibrogenic behaviors of theα-TN4 lens epithelial cell line[J]. J Cataract Refract Surg,2006,32(10):1727-1735.
[13]刘臻,张铭倩,朱虹.栝楼瞿麦汤对糖尿病肾病大鼠脂肪因子和足细胞nephrin的影响[J].中国实验方剂学杂志,2017,23(23):134-139.
[14]段晓颖,高卫芳,吴彩丽,等.均匀设计法优选胡黄连中胡黄连苷的提取工艺[J].中国药房,2011,22(39):3675-3676.
[15] Kumar V,Sood H,Chauhan R S. Optimization of a preparative RP-HPLC method for isolation and purification of picrosides in Picrorhiza kurroa[J]. J Plant Biochem Biot,2016,25(2):208-214.