摘要
为在刺槐育种研究中合理选用不同来源的SSR分子标记,选取12个刺槐个体,试剂盒提取DNA后分别利用9对Genomic-SSR引物和9对EST-SSR引物进行扩增,并采取毛细管电泳检测扩增产物。利用所得条带信息及相关软件对2种SSR分子标记进行多态性、遗传相似系数相关性以及聚类等方面的比较分析。结果显示,刺槐Genomic-SSR平均检测到的条带数为6、Shannon多样性指数为1.3833、观测杂合度为0.5749、期望杂合度为0.6832;EST-SSR平均检测到的条带数为5.1、Shannon多样性指数为1.2711、观测杂合度为0.5648、期望杂合度为0.6526。由Genomic-SSR计算得到的个体间的遗传相似系数以及聚类结果与2种SSR标记综合计算得到的遗传相似系数和聚类结果更为相似。结果表明,刺槐Genomic-SSR与EST-SSR存在一定的遗传差异性,但差异并不显著;刺槐Genomic-SSR能更加准确地揭示基因型之间的遗传关系;刺槐EST-SSR具有相对较强的保守性。
The study aims at selecting the SSR molecular markers reasonably in the breeding of black locust.12 individuals of black locust were used as the materials, the total genomic DNA was extracted by kit. 9 Genomic-SSR and 9 EST-SSR primers were used for PCR amplification and the products were separated by capillary electrophoresis. Comparative analysis of polymorphism, correlation of genetic similarity coefficients and cluster analysis between Genomic-SSR and EST-SSR were conduct by related software according to the information of the electrophoretic bands. The average number of bands detected by Genomic-SSR was 6.0,Shannon index was 1.3833, observed heterozygosity was 0.5749 and expected heterozygosity was 0.6832. The average number of bands detected by EST-SSR was 5.1, Shannon index was 1.2711, observed heterozygosity was 0.5648 and expected heterozygosity was 0.6526. Genetic similarity coefficients and results of cluster analysis between individuals calculated from Genomic-SSR were more similar to the comprehensive results calculated from the two kinds of SSR markers. There are genetic differences between Genomic-SSR and ESTSSR of black locust, but the differences are not significant. Genomic-SSR of black locust can more accurately reflect genetic relationship of different genotypes. EST-SSR of black locust is relatively more conservative.
引文
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