5个南方型杨树无性系苗木耐盐性盆栽试验
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摘要
[目的]研究盐胁迫对杨树生长及生理指标的影响,开展不同杨树无性系耐盐性比较及评价。[方法]以895杨(Populus×euramericana‘Nanlin 895’)、1388杨(P.×euramericana‘Nanlin 1388’)、3804杨(P.deltoides‘Nanlin 3804’)、110杨(P.deltoides×P.cathayana cl.‘110’)和328杨(P.×euramericana cl.‘328’)5个杨树无性系为材料,采用室内盆栽方法,以无盐处理为对照,对其在不同盐(NaCl)浓度处理(1‰、2‰、3‰、4‰、5‰)土壤下的成活率、生长量、叶片细胞质膜透性、丙二醛含量、光合速率等13项指标进行测定和分析,采用隶属函数法对其耐盐性进行了综合评价。[结果]在土壤盐胁迫下5个杨树无性系的苗高、地径和生物量生长均受到了抑制;苗木成活率、叶片光合速率和叶绿素含量均随盐处理浓度的提高而降低;叶片细胞质膜透性和丙二醛含量随着盐处理浓度的提高而提高。[结论]5个杨树无性系耐盐性存在差异,其顺序为:110杨>328杨>3804杨>895杨>1388杨。110杨能耐受3‰左右的土壤含盐量,其它无性系只能耐受2‰左右。
[Objective] To study the effects of salt stress on growth and physiological indicators of poplar and to compare the salt tolerance among poplar clones. [Method] The seedlings of five poplar clones, i.e. Nanlin-895(Populus × euramericana ‘Nanlin 895'), Nanlin-1388(P. × euramericana ‘Nanlin 1388'), Nanlin-3804(P. deltoides ‘Nanlin 3804'), 110(P. deltoides ×P. cathayana cl. ‘110') and 328(P. × euramericana cl. ‘328') were selected to analyze the survival rate, growth traits, cell membrane permeability, malondialdehyde content and photosynthetic rate treated with 1‰, 2‰, 3‰, 4‰, 5‰ of salt(NaCl) and the CK in a greenhouse. The salt tolerance of clones was comprehensively evaluated by subordinate function method. [Result] The height, ground diameter and biomass of all the poplar clones seedling were inhibited under the salt treatments. The survival rate, net photosynthetic rate and chlorophyll content of seedlings decreased with increasing salt concentration, while the cell membrane permeability and malondialdehyde content in leave increased with increasing salt concentration. [Conclusion] The salt tolerance of the five clones is in the order of clone 110 > clone 328 > Nanlin-3804 > Nanlin-895 > Nanlin-1388. Clone 110 can tolerate the soil with a salt concentration of 3‰ while the other clones can tolerate the soil with a salt concentration of 2‰.
[Objective] To study the effects of salt stress on growth and physiological indicators of poplar and to compare the salt tolerance among poplar clones. [Method] The seedlings of five poplar clones, i.e. Nanlin-895(Populus × euramericana ‘Nanlin 895'), Nanlin-1388(P. × euramericana ‘Nanlin 1388'), Nanlin-3804(P. deltoides ‘Nanlin 3804'), 110(P. deltoides ×P. cathayana cl. ‘110') and 328(P. × euramericana cl. ‘328') were selected to analyze the survival rate, growth traits, cell membrane permeability, malondialdehyde content and photosynthetic rate treated with 1‰, 2‰, 3‰, 4‰, 5‰ of salt(NaCl) and the CK in a greenhouse. The salt tolerance of clones was comprehensively evaluated by subordinate function method. [Result] The height, ground diameter and biomass of all the poplar clones seedling were inhibited under the salt treatments. The survival rate, net photosynthetic rate and chlorophyll content of seedlings decreased with increasing salt concentration, while the cell membrane permeability and malondialdehyde content in leave increased with increasing salt concentration. [Conclusion] The salt tolerance of the five clones is in the order of clone 110 > clone 328 > Nanlin-3804 > Nanlin-895 > Nanlin-1388. Clone 110 can tolerate the soil with a salt concentration of 3‰ while the other clones can tolerate the soil with a salt concentration of 2‰.
引文
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[16] 孙海菁,王树凤,陈益泰.盐胁迫对6个树种的生长及生理指标的影响[J].林业科学研究,2009,22(3):315-324.
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[18] 邱凤英,廖宝文,肖复明.半红树植物杨叶肖槿幼苗耐盐性研究[J].林业科学研究,2011,24(1):51-55.
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[21] 杨传平,焦喜才,刘文祥,等.树木的细胞膜透性与抗盐性[J].东北林业大学学报,1997,25(1):1-3.
[22] 杨发荣,刘文瑜,黄杰,等.不同藜麦品种对盐胁迫的生理响应及耐盐性评价[J].草业学报,2017,26(12):77-88.
[23] 吴永波,薛建辉.盐胁迫对3种白蜡树幼苗生长与光合作用的影响[J].南京林业大学学报:自然科学版,2002,26(3):19-22.
[24] 王臣,虞木奎,张翠,等.盐胁迫下3个楸树无性系光合特征研究[J].林业科学研究,2010,23(4):537-543
[25] 赵可夫.植物耐盐生理[M].北京:中国科学技术出版社,1993.
[26] 汪贵斌,曹福亮.盐分和水分胁迫对落羽杉幼苗的生长量及营养元素含量的影响[J].林业科学,2004,40(6):56-62.
[2] 张建锋,张旭东,周金星,等.盐分胁迫对杨树苗期生长和土壤酶活性的影响[J].应用生态学报,2005,16(3):426-430.
[3] 徐彩平.南林895杨组培苗耐盐性及耐盐机制的研究[D].南京:南京林业大学,2012.
[4] Yu L,Ma J C,Niu Z M,et al.Tissue-specific transcriptome analysis reveals multiple responses to salt stress in Populus euphratica seedlings[J].Genes,2017,8:372.
[5] Chen J F,Zhang J,Hu J J,et al.Integrated regulatory network reveals the early salt tolerance mechanism of Populus euphratica[J].Scientific Reports,2017,7:6769.
[6] 綦艳林,朱雅娟,贾志清,等.荒漠河岸林2种典型植物的耐盐性比较[J].林业科学研究,2011,24(3):327-333.
[7] 罗子敬,孙宇涵,卢楠,等.杨树耐盐机制及转基因研究进展[J].核农学报,2017,31(3):482-492.
[8] 陈盼飞,左力辉,王桂英,等.盐胁迫下转复合多基因欧美杨107杨幼苗生长及生理响应[J].林业科学,2017,53(7):45-53.
[9] 孙伟博,邓大霞,杨立恒,等.南林895杨树GmNHX1基因的转入及其耐盐性分析[J].福建农林大学学报:自然科学版,2014,43(1):34-38.
[10] 张志良.植物生理学实验指导[M].北京:高等教育出版社,1990.
[11] 郑炳松.现代植物生理生化研究技术[M].北京:气象出版社,2006.
[12] 杨敏生,裴保华,朱之悌.白杨双交杂种无性系抗旱性鉴定指标分析[J].林业科学,2002,38(6):36-42.
[13] 李二龙.四种木本植物对铅胁迫的生理响应[D].成都:四川农业大学,2009.
[14] 支晓蓉,杨秀艳,任坚毅,等.我国园林植物耐盐性评价及鉴定研究进展[J].世界林业研究,2018,31(5):51-57.
[15] 杨少辉,季静,王罡,等.盐胁迫对植物影响的研究进展[J].分子植物育种,2006,4(3):139-142.
[16] 孙海菁,王树凤,陈益泰.盐胁迫对6个树种的生长及生理指标的影响[J].林业科学研究,2009,22(3):315-324.
[17] 张华新,宋丹,刘正祥.盐胁迫下11个树种生理特性及其耐盐性研究[J].林业科学研究,2008,21(2):168-175.
[18] 邱凤英,廖宝文,肖复明.半红树植物杨叶肖槿幼苗耐盐性研究[J].林业科学研究,2011,24(1):51-55.
[19] Mansour M M F,Salama K H A.Cellular basis of salinity tolerance in plants[J].Environmental & Experimental Botany,2004,52(2):113-122.
[20] 李倩,刘景辉,武俊英,等.盐胁迫对燕麦质膜透性及Na+、K+吸收的影响[J].华北农学报,2009,24(6):88-92.
[21] 杨传平,焦喜才,刘文祥,等.树木的细胞膜透性与抗盐性[J].东北林业大学学报,1997,25(1):1-3.
[22] 杨发荣,刘文瑜,黄杰,等.不同藜麦品种对盐胁迫的生理响应及耐盐性评价[J].草业学报,2017,26(12):77-88.
[23] 吴永波,薛建辉.盐胁迫对3种白蜡树幼苗生长与光合作用的影响[J].南京林业大学学报:自然科学版,2002,26(3):19-22.
[24] 王臣,虞木奎,张翠,等.盐胁迫下3个楸树无性系光合特征研究[J].林业科学研究,2010,23(4):537-543
[25] 赵可夫.植物耐盐生理[M].北京:中国科学技术出版社,1993.
[26] 汪贵斌,曹福亮.盐分和水分胁迫对落羽杉幼苗的生长量及营养元素含量的影响[J].林业科学,2004,40(6):56-62.