心痛宁加味方对蒽环类药物表柔比星诱导小鼠急性心肌损害的保护作用
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摘要
目的探讨心痛宁加味方治疗表柔比星诱导小鼠急性心肌损害的保护作用。方法将60只实验小鼠随机分为6组,10只/组。空白对照组小鼠经腹腔注射生理盐水,模型组小鼠经腹腔内注射表柔比星与生理盐水,丹参组小鼠经腹腔内注射表柔比星与丹参,其他组分别为表柔比星联合低、中、高剂量的心痛宁加味方,处死小鼠后检测脑钠肽(BNP)、心型脂肪酸结合蛋白(h-FABP)、一氧化氮(NO)、超氧化物歧化酶(SOD)活性、肌钙蛋白I(cTnI)水平,同时观察各组小鼠心肌组织的病理形态学改变以及心肌细胞凋亡情况。结果各组BNP、h-FABP、NO、SOD活性、cTnI指标比较,存在显著差异(P <0.05);模型组心肌细胞病例评分明显高于丹参组、低、中、高剂量心痛宁加味方组,并且各组心肌细胞凋亡指数比较存在显著差异(P <0.05);以模型组心肌损害、凋亡效果最为严重,除对照组外以丹参组与高剂量组心肌损害、凋亡最轻。结论心痛宁加味方及丹参对表柔比星诱导的小鼠心肌损害具有良好的保护作用,其可有效降低氧自由基的产生,并通过降低脂质过氧化物含量达到改善心肌损害及调节凋亡的目的。
Objective To investigate the protective effect of Xintongning Jiawei Formula on acute myocardial damage induced by epirubicin in mice. Methods Sixty experimental mice were randomly divided into 6 groups with 10 mice in each group. The mice in the blank control group received intraperitoneal injection of saline, the mice in the model group received intraperitoneal injection of epirubicin and saline, and the mice in the Danshen(Radix Salviae Miltiorrhizae) group received intraperitoneal injection of epirubicin and Danshen. Other groups were treated with epirubicin combined with low, medium and high dose Xintongning Jiawei Formula. The brain natriuretic peptide(BNP), cardiac fatty acid-binding protein(h-FABP), nitric oxide(NO), superoxide dismutase(SOD) activity and troponin I(cTnI) levels were detected after the mice were killed. At the same time, the histopathological changes and myocardial cell apoptosis were observed in all groups. Results There were significant differences in the levels of BNP, h-FABP, NO, SOD activity and cTnI among the groups(P < 0.05); the case score of myocardial cells in the model group was significantly higher than that in the Danshen group, low, medium, and high dose Xintongning Jiawei Formula groups. There were significant differences in apoptotic index of myocardial cells among groups(P < 0.05). The effect of myocardial damage and apoptosis was the most serious in the model group. Except for the control group, the effect of myocardial damage and apoptosis was the least in the Danshen group and the high dose Xintongning Jiawei Formula group. Conclusion Xintongning Jiawei Formula combined with Danshen has a good protective effect on myocardial damage induced by epirubicin in mice. It can effectively reduce the production of oxygen free radicals, and improve myocardial damage and regulate apoptosis by reducing lipid peroxide content.
Objective To investigate the protective effect of Xintongning Jiawei Formula on acute myocardial damage induced by epirubicin in mice. Methods Sixty experimental mice were randomly divided into 6 groups with 10 mice in each group. The mice in the blank control group received intraperitoneal injection of saline, the mice in the model group received intraperitoneal injection of epirubicin and saline, and the mice in the Danshen(Radix Salviae Miltiorrhizae) group received intraperitoneal injection of epirubicin and Danshen. Other groups were treated with epirubicin combined with low, medium and high dose Xintongning Jiawei Formula. The brain natriuretic peptide(BNP), cardiac fatty acid-binding protein(h-FABP), nitric oxide(NO), superoxide dismutase(SOD) activity and troponin I(cTnI) levels were detected after the mice were killed. At the same time, the histopathological changes and myocardial cell apoptosis were observed in all groups. Results There were significant differences in the levels of BNP, h-FABP, NO, SOD activity and cTnI among the groups(P < 0.05); the case score of myocardial cells in the model group was significantly higher than that in the Danshen group, low, medium, and high dose Xintongning Jiawei Formula groups. There were significant differences in apoptotic index of myocardial cells among groups(P < 0.05). The effect of myocardial damage and apoptosis was the most serious in the model group. Except for the control group, the effect of myocardial damage and apoptosis was the least in the Danshen group and the high dose Xintongning Jiawei Formula group. Conclusion Xintongning Jiawei Formula combined with Danshen has a good protective effect on myocardial damage induced by epirubicin in mice. It can effectively reduce the production of oxygen free radicals, and improve myocardial damage and regulate apoptosis by reducing lipid peroxide content.
引文
[1]谢文卫,吴雪琴,周益萍,等.表柔比星相关心肌损伤患者血清NT-proBNP改变及临床意义[J].医学综述, 2015,21(22):4197-4199.
[2]张淑娟,王振涛,韩丽华,等.丹参注射液对心肌梗死后大鼠缺血心肌血管新生的影响[J].中医杂志, 2011, 35(18):1590-1592.
[3]吴晖,欧阳取长,李晶,等.右丙亚胺对表柔比星诱导大鼠心肌损伤干预机制研究[J].中华肿瘤防治杂志, 2013,20(19):1473-1477.
[4]周捷,黄海潮,聂阳,等.甘木通总黄酮对表柔比星心肌毒性的抑制作用及机制研究[J].中药材, 2017, 40(2):436-440.
[5]张蕴慧.心痛宁胶囊治疗冠状动脉痉挛阳虚血瘀证患者高敏C反应蛋白水平的观察[J].中西医结合心脑血管病杂志,2012, 10(3):269-271.
[6]王琼,蒿业红,李艳霞.复方丹参滴丸对老年晚期乳腺癌放化疗后患者心功能下降的保护作用[J].中国生化药物杂志, 2017, 22(4):109-111.
[7]张淑玲,孟哲颖,陈翠,等.速度向量成像技术评估蒽环类药物所致心肌损害的临床价值[J].中国医学前沿杂志(电子版), 2017, 9(5):67-74.
[8]齐宇新,刘姣,何颖娜,等.乳腺癌术后蒽环类化疗药物致心脏损害的早期监测分析[J].中国药房, 2017, 28(17):2356.
[9]刘剑刚,张大武,李婕,等.丹参、红花水溶性组分及配伍对大鼠心肌缺血/再灌注损伤作用的实验研究[J].中国中药杂志, 2011, 36(2):189-194.
[10]李增才,刘现忠,王兵济.丹参酮IIA磺酸钠注射液联合阿霉素对化疗致心肌损伤的疗效观察[J].实用临床医药杂志, 2016, 20(7):170-171.
[11]黄邹琴,柳子川,李涛,等.蒽环类药物化疗对乳腺癌患者心肌损伤相关因素的影响[J].广东药学院学报, 2016,32(3):383-386.
[12]刘颖,姜毅,徐岳.益气活血方改善蒽环类化疗药物致慢性心肌损害症状的临床研究[J].云南中医中药杂志,2016, 37(12):51-52.
[13]姚铁柱,徐志宏,马景涛,等.参麦注射液对乳腺癌患者术后化疗导致心肌损害的保护作用[J].中国实验方剂学杂志, 2016, 40(7):208-212.
[14]杨邵瑜,陈雪琴,潘月龙,等.参附注射液防治表柔比星心肌毒性疗效观察[J].中国中医急症, 2010, 19(8):1317-1318.
[15]李永胜,严丽,雍永权,等.丹参酮ⅡA对高血压大鼠肥厚心肌TGF-β1/Smads信号通路的影响[J].中国中西医结合杂志, 2010, 30(5):499-503.
[2]张淑娟,王振涛,韩丽华,等.丹参注射液对心肌梗死后大鼠缺血心肌血管新生的影响[J].中医杂志, 2011, 35(18):1590-1592.
[3]吴晖,欧阳取长,李晶,等.右丙亚胺对表柔比星诱导大鼠心肌损伤干预机制研究[J].中华肿瘤防治杂志, 2013,20(19):1473-1477.
[4]周捷,黄海潮,聂阳,等.甘木通总黄酮对表柔比星心肌毒性的抑制作用及机制研究[J].中药材, 2017, 40(2):436-440.
[5]张蕴慧.心痛宁胶囊治疗冠状动脉痉挛阳虚血瘀证患者高敏C反应蛋白水平的观察[J].中西医结合心脑血管病杂志,2012, 10(3):269-271.
[6]王琼,蒿业红,李艳霞.复方丹参滴丸对老年晚期乳腺癌放化疗后患者心功能下降的保护作用[J].中国生化药物杂志, 2017, 22(4):109-111.
[7]张淑玲,孟哲颖,陈翠,等.速度向量成像技术评估蒽环类药物所致心肌损害的临床价值[J].中国医学前沿杂志(电子版), 2017, 9(5):67-74.
[8]齐宇新,刘姣,何颖娜,等.乳腺癌术后蒽环类化疗药物致心脏损害的早期监测分析[J].中国药房, 2017, 28(17):2356.
[9]刘剑刚,张大武,李婕,等.丹参、红花水溶性组分及配伍对大鼠心肌缺血/再灌注损伤作用的实验研究[J].中国中药杂志, 2011, 36(2):189-194.
[10]李增才,刘现忠,王兵济.丹参酮IIA磺酸钠注射液联合阿霉素对化疗致心肌损伤的疗效观察[J].实用临床医药杂志, 2016, 20(7):170-171.
[11]黄邹琴,柳子川,李涛,等.蒽环类药物化疗对乳腺癌患者心肌损伤相关因素的影响[J].广东药学院学报, 2016,32(3):383-386.
[12]刘颖,姜毅,徐岳.益气活血方改善蒽环类化疗药物致慢性心肌损害症状的临床研究[J].云南中医中药杂志,2016, 37(12):51-52.
[13]姚铁柱,徐志宏,马景涛,等.参麦注射液对乳腺癌患者术后化疗导致心肌损害的保护作用[J].中国实验方剂学杂志, 2016, 40(7):208-212.
[14]杨邵瑜,陈雪琴,潘月龙,等.参附注射液防治表柔比星心肌毒性疗效观察[J].中国中医急症, 2010, 19(8):1317-1318.
[15]李永胜,严丽,雍永权,等.丹参酮ⅡA对高血压大鼠肥厚心肌TGF-β1/Smads信号通路的影响[J].中国中西医结合杂志, 2010, 30(5):499-503.