环氧合酶-2和磷酸化P38丝裂原活化蛋白激酶在扁平苔藓皮损中的表达
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摘要
目的:检测环氧合酶-2(COX-2)及其上游调控蛋白磷酸化P38丝裂原活化蛋白激酶(p-P38MAPK)在扁平苔藓皮损中的表达,探讨其在发病中的意义。方法:采用免疫组化技术检测20例扁平苔藓皮损标本中COX-2和p-P38MAPK的表达情况,20例正常皮肤组织作为对照。结果:20例扁平苔藓皮损标本中COX-2和p-P38MAPK的积分光密度值(IOD)分别为(8.46±1.79)×10~3和(11.54±2.85)×10~3,均明显高于对照组(3.14±2.35)×10~3和(6.26±3.12)×10~3,差别具有统计学意义(均P<0.01),同时COX-2与p-P38MAPK的表达强度呈正相关(r=0.62,P<0.01)。结论:扁平苔藓皮损中COX-2表达上调,可能与上游P38MAPK的活化有关,可能是引起扁平苔藓发病的环节之一。
Objective: To detect the expression level of cyclooxygenase-2(COX-2) and phosphorylated P38 mitogen-activated protein kinase(p-P38 MAPK) in the lesions of lichen planus(LP) and to explore its role in the pathogenesis of LP. Methods: The expression level of COX-2 and p-P38 MAPK in 20 lesions with LP and normal skin of 20 human controls were detected by immunohistochemical technology. Results: The IOD(integrated optical density) of COX-2 and p-P38 MAPK in LP lesions were(8.46±1.79)×10~3 and(11.54±2.85)×10~3, which were both higher than those in the controls,(3.14±2.35)×10~3 and(6.26±3.12)×10~3, with significant differences(P_s<0.01). There was a positive correlation between the expression level of COX-2 and p-P38 MAPK in LP lesions(r=0.62,P<0.01). Conclusion: The up-regulation of COX-2 expression may be correlated with the activation of upstream P38 MAPK, which may be involved in the pathogenesis of lichen planus.
Objective: To detect the expression level of cyclooxygenase-2(COX-2) and phosphorylated P38 mitogen-activated protein kinase(p-P38 MAPK) in the lesions of lichen planus(LP) and to explore its role in the pathogenesis of LP. Methods: The expression level of COX-2 and p-P38 MAPK in 20 lesions with LP and normal skin of 20 human controls were detected by immunohistochemical technology. Results: The IOD(integrated optical density) of COX-2 and p-P38 MAPK in LP lesions were(8.46±1.79)×10~3 and(11.54±2.85)×10~3, which were both higher than those in the controls,(3.14±2.35)×10~3 and(6.26±3.12)×10~3, with significant differences(P_s<0.01). There was a positive correlation between the expression level of COX-2 and p-P38 MAPK in LP lesions(r=0.62,P<0.01). Conclusion: The up-regulation of COX-2 expression may be correlated with the activation of upstream P38 MAPK, which may be involved in the pathogenesis of lichen planus.
引文
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[2] 王娟,白莉,鲍海平,等.扁平苔藓中Ki-67和VEGF的表达[J].实用医学杂志,2012,28(6):916-918.
[3] 冯珺,白莉,张学良.扁平苔藓皮损中缺氧诱导因子1α、血管内皮生长因子和蛋白激酶B的表达[J].中华皮肤科杂志,2017,50(1):18-21.
[4] El-Rifaie AA,Rashed LA,Doss RW.The role of cyclooxygenase-2 and prostaglandin E2 in the pathogenesis of cutaneous lichen planus[J].Clin Exper Dermatol,2015,40(8):903-907.
[5] Roseweir AK,Powell AG,Bennett L,et al.Relationship between tumour PTEN/Akt/COX-2 expression,inflammatory response and survival in patients with colorectal cancer[J].Oncotarget,2016,7(43):70601-70612.
[6] Gesser B,Rasmussen MK,Raaby L,et al.Dimethylfumarate inhibits MIF-induced proliferation of keratinocytes by inhibiting MSK1 and RSK1 activation and by inducing nuclear p-c-Jun(S63) and p-p53(S15) expression[J].Inflamm Res,2011,60(7):643-653.
[7] 臧家兵,王洪远,王伟,等.COX-2和MMP-9在皮肤鳞状细胞癌中的表达[J].中国麻风皮肤病杂志,2012,28(7):482-484.
[8] 黄艳丽,梁虹.COX-2在银屑病皮损中的表达及其与血管新生的关系[J].中国麻风皮肤病杂志,2010,26(4):249-251.
[9] 王永华,柳志文.COX-2、VEGF在口腔扁平苔藓、口腔白斑及口腔鳞状细胞癌中的表达[J].口腔医学,2009,29(8):419-422.
[10] Moon YJ,Lee JY,Oh MS,et al.Inhibition of inflammation and oxidative stress by Angelica dahuricae radix extract decreases apoptotic cell death and improves functional recovery after spinal cord injury[J].J Neurosci Res,2012,90(1):243-256.
[11] Kumar RP,Abraham A.Inhibition of LPS induced pro-inflammatory responses in RAW 264.7 macrophage cells by PCP-coated naringenin nanoparticle via down regulation of NF-κB/P38MAPK mediated stress signaling[J].Pharmacol Rep,2017,69(5):908-915.
[12] Limtrakul P,Yodkeeree S,Pitchakarn P,et al.Suppression of inflammatory responses by black rice extract in RAW264.7 macrophage cells via downregulation of NF-κB and AP-1 signaling pathways[J].Asian Pacific J Cancer Prevention,2015,16(10):4277-4283.
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